Epitopes from allergen proteins and methods and uses for immune response modulation

ABSTRACT

The invention relates to common allergen proteins and peptides, subsequences, portions, homologues, variants and derivatives thereof, and methods and uses of common allergen proteins and peptides. Methods include, for example, modulating an immune response; protecting a subject against or treating a subject for an allergic response, allergic disorder or allergic disease; and inducing immunological tolerance to the allergen in a subject.

RELATED APPLICATION INFORMATION

This application claims priority to application Ser. No. 61/596,166, filed Feb. 7, 2012, and application Ser. No. 61/642,372, filed May 3, 2012, both of which applications are expressly incorporated herein by reference in their entirety.

GOVERNMENT SUPPORT

This invention received government support from the National Institutes Health contract NIAIDHHSN272200700048C and grant U19AI100275. The government has certain rights in the invention.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Feb. 6, 2013, is named 051501-0420333_SL.txt and is 405,771 bytes in size.

INTRODUCTION

Allergic reactions to common environmental allergens are associated with serious clinical manifestations, such as rhinitis and asthma, translating into high morbidity and societal costs. Furthermore, the incidence and prevalence of allergic disease is constantly rising. Current treatments for allergic disease are not fully satisfactory and, coincidentally, understanding of the fundamental aspects of allergic disease remains incomplete (1, 2).

Initiation and maintenance of allergic disease is due to a complex series of molecular events, including both innate and adaptive immunity. In terms of adaptive immunity, IgE is of particular importance. Its cross-linking by antigen is a main cause of the release of histamine and other mediators, and subsequent clinical manifestations of an allergic response. In fact, IgE reactivity is utilized to define the particular allergens contained in a given allergen source. However, in addition to IgE responses, T cells also vitally contribute to allergic disease. This contribution may be indirect, by promoting the production of IgE and the differentiation of eosinophils, or direct, through the release of various pro-inflammatory cytokines, such as IL-5, which promotes eosinophilic inflammation. A possible beneficial role of regulatory T cells (Tregs), resulting in suppression of allergic reactions, has also been indicated by several studies. Knowledge of the role of antigen specific T cells in allergic reactions in humans is limited, and detailed studies are hampered by a relative paucity of knowledge relating to the epitopes recognized by allergen-specific T cells.

Although there are reports of epitopes recognized by human T cells, for many allergen systems the information is either fragmentary or lacking altogether. Responses to complex allergens in humans are very heterogeneous and involve recognition of a large number of epitopes (3-18). At the same time, the most dominant and prevalent responses encompass a significant fraction of the response, and these dominant epitopes can be predicted on the basis of their capacity to bind, and be recognized in the context of, multiple HLA DR, DP and DQ allelic variants (3).

As disclosed herein, a large panel of allergen proteins derived from 28 common allergen sources, which included fungi (Alternaria, Aspergillus, Cladosporuim and Penicillium), trees (Alder, Ash, Birch, Black Walnut, Cypress, Juniper, Oak and Palm), grasses (Bermuda, Canary, Kentucky Blue, Orchard, Rye and Sweet Vernal), weeds (English Plantain, Giant Ragweed, Mugwort, Russian Thistle, and Western Ragweed) and various indoor allergens (American Cockroach, Cat dander, Dog dander and Dust Mites) was probed. Over 250 different antigenic regions were identified and provide the first actual epitope data for several allergen sources. As also disclosed herein, T cell responses to the allergens previously defined on the basis of IgE reactivity account for a variable, and in many cases a surprisingly small, fraction of T cell responses, suggesting that several antigens involved in T cell recognition are yet to be described.

SUMMARY

A panel of 133 allergens derived from 28 different sources, including fungi, trees, grasses, weeds and indoor allergens, was surveyed utilizing prediction of HLA class II binding peptides and ELISPOT assays with PBMC from allergic donors, resulting in the identification of 257 T cell epitopes. More than 90% of the epitopes were novel, and for 14 allergen sources were the first ever identified. The epitopes identified in the different allergen sources summed up to a variable fraction of the total extract response. In cases of allergens where the identified T cell epitopes accounted for a minor fraction of the extract response, fewer known protein sequences were available, suggesting that for “low epitope coverage” allergen sources, additional allergen proteins remain to be identified. IL-5 and IFN-γ responses were measured as prototype Th2 and Th1 responses, respectively. While in some cases (e.g., Orchard Grass, Alternaria, Cypress, and Russian Thistle) IL-5 production greatly exceeded IFN-γ, in others (e.g., Aspergillus, Penicillum, and Alder) the production of IFN-γ exceeded IL-5. Thus, different allergen sources are differentially polarized in terms of their capacity to recall production of Th1 versus Th2 associated lymphokines, and are associated with variable polarization of the responding T cells.

Disclosed herein is the most comprehensive survey of human allergen derived T cell epitopes. These epitopes can be used to characterize T cell responsiveness, T cell phenotype/T cell PLASTICITY as a function of seasonality, or as a result of SIT treatment or varying disease severity (asthma or rhinitis). In addition, these epitopes of allergens can be used in various clinical applications, for example, to modulate a T cell response or activity, to elicit, stimulate, induce, promote, increase or enhance an anti-allergen immune response, and to decrease, inhibit, suppress or reduce an anti-allergen immune response.

In accordance with the invention, provided are proteins and peptides that include, consist of or consist essentially of an amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance), or a variant thereof or derivative thereof. In accordance with the invention, also provided are proteins and peptides that include, consist of or consist essentially of an amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), or a variant or derivative thereof.

Proteins and peptides include subsequences, portions, homologues, variants, and derivatives of an amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In particular aspects, a subsequence, portion, homologue, variant or derivative is: a peptide of up to 30 amino acids in length and which comprises said amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance).). In other particular aspects, a subsequence, portion, homologue, variant or derivative is: a peptide from 7 to 30 amino acids in length, wherein at least 7 contiguous amino acids have at least 75% identity to at least 7 contiguous amino acids of said amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance).

In further embodiments, a protein or peptide does not consist of the sequence of Der f 1, Der f 2, Der p 1 and Der p 2 set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance). In other embodiments, a protein or peptide is derived from a house dust mite allergen (e.g., an amino acid sequence of Der f 10 (SEQ ID NOs:568-570), Der f 11 (SEQ ID NO:1571), Der f 13 (SEQ ID NOs:1572-1575), Der f 14 (SEQ ID NO:1576), Der f 16 (SEQ ID NOs:1577-1584), Der f 18 (SEQ ID NOs:1585-1586), Der p 3 (SEQ ID NOs:1609-1614), Der p 4 (SEQ ID NOs:1615-1646), Der p 5 (SEQ ID NO:647), Der p 9 (SEQ ID NOs:1648-1650) or Der p 14 (SEQ ID NOs:1604-1607) as specified in Table 7.

Proteins and peptides include allergen or antigen, and subsequences, portions, homologues, variants, and derivatives thereof. Proteins and peptides also include an amino acid sequence, variant or derivative thereof that elicits, stimulates, induces, promotes, increases or enhances an anti-allergen immune response. Proteins and peptides further include an amino acid sequence, variant or derivative thereof decreases, inhibits, suppresses or reduces an anti-allergen immune response (e.g., a T cell response or production of IgE antibody, stimulated, induced, increased or enhanced production of a lymphokine). Proteins and peptides moreover include an amino acid sequence, variant or derivative thereof that modulates a Th2 immune response. Proteins and peptides additionally include an amino acid sequence, variant or derivative thereof modulates production of a lymphokine or cytokine by a cell. Proteins and peptides still further include an amino acid sequence, variant or derivative thereof desensitizes, or improves, increases, or induces immunological tolerance of a subject to the allergen (e.g., to which the subject has been sensitized or is hypersensitive).

Proteins and peptides still moreover include an amino acid sequence, variant or derivative thereof modulates production of IL-5 (interleukin-5), IL-4 (interleukin-4), IL-10 (interleukin-10), IL-13 (interleukin-13), IL-17 (interleukin-17), or IFN-γ (interferon-gamma). Proteins and peptides yet additionally include an amino acid sequence, variant or derivative thereof increases, induces, elicits, or stimulates a Th2 immune response; increases, induce, elicits or stimulates production of a lymphokine by a cell; or increases, induces, elicits or stimulates production of IL-5 (interleukin-5), IL-4 (interleukin-4), IL-10 (interleukin-10), IL-13 (interleukin-13), IL-17 (interleukin-17), or IFN-γ (interferon-gamma). Such production can optionally be detected by an immunoassay, or is determined by contacting peripheral blood mononuclear cells (PBMC), such as PBMC's obtained from a subject allergic to an allergen or an organism from which the protein or peptide derives, with the protein or peptide followed by an immunoassay.

As disclosed herein, certain proteins and peptides include, consist of or consist essentially of an allergen or antigen. In particular aspects, an allergen or antigen is derived from or produced by Alder, Alternaria rot fungus, American cockroach, Dust mite, Ash, Aspergillus fumigatus, Bermuda grass, Birch, Black Walnut, Canary grass, Cat, Cladosporium herbarum, Common cypress, Cypress, Palm, Dog, English plantain, Giant ragweed, Japanese cypress, Kentucky blue grass, Lolium perenne, Mugwort weed, Orchard grass, Penicillium chrysogenum, Prickly juniper, Russian thistle, Rye grass, Sweet vernal grass, Western Ragweed or White oak.

As disclosed herein, in certain embodiments proteins and peptides have a length in a range of about 5-10, 10-15, 15-20, 20-25, 25-30, 30-35, 35-40, 45-50, 50-60, 60-70, 70-80, 90-100, 100-125, 125-150, 150-175, 175-200, 200-250, 250-300, or more amino acid residues. In other embodiments, proteins and peptides have a length in a range of up to 25 amino acids in length, or from about 7 to 20; 8 to 30; 8 to 25; 8 to 20; 9 to 30; 9 to 25; 9 to 20; 10 to 30; 10 to 25; 10 to 30 amino acid residues.

Proteins and peptides include isolated and purified forms. Proteins and peptides also include those immobilized on a substrate, as well as amino acid sequences, subsequences, portions, homologues, variants, and derivatives immobilized on a substrate.

Proteins and peptides can be included in compositions, for example, a pharmaceutical composition. In particular embodiments, a pharmaceutical composition is suitable for specific or non-specific immunotherapy, or is a vaccine composition

Isolated nucleic acid (including isolated nucleic acid) encoding the proteins and peptides are also provided. Cells expressing a protein or peptide are further provided. Such cells include eukaryotic and prokaryotic cells, such as mammalian, insect, fungal and bacterial cells.

Methods and uses and medicaments of proteins and peptides of the invention are included. Such methods, uses and medicaments include modulating immune activity of a cell against an allergen; and desensitizing, inducing, eliciting, increasing or improving in the cell immunological tolerance to an allergen. In particular embodiments, a method or use includes contacting a cell with an amount of the protein or peptide of any one of the above-mentioned embodiments, sufficient to modulate the immune activity of the cell against the allergen (e.g., against an allergen from which the peptide or protein derives), or administering to a subject an allergen from which the peptide or protein derives in order to desensitize, induce, elicit, increase or improve immunological tolerance to the allergen or to modulate an immune response against an allergen in a subject (e.g., an allergen from which the peptide or protein derives).

Such methods, uses and medicaments also include reducing risk or providing a subject protection against an allergic reaction, allergic response, allergic disorder or allergic disease. In one embodiment, a method or use includes administering to the subject an amount of the protein or peptide sufficient to reduce risk or provide the subject with protection against the allergic reaction, allergic response, allergic disorder or allergic disease. Non-limiting examples of an allergic reaction or allergic response include allergic alveolitis, allergic bronchopulmonary aspergillosis, allergic conjunctivitis, allergic coryza, allergic dermatitis, allergic vasculitis, and allergic rhinitis.

Such methods, uses and medicaments further include treating an allergic reaction, allergic response, allergic disorder or allergic disease. In one embodiment, a method or use includes administering to the subject an amount of the protein or peptide, sufficient to treat the subject for the allergic response, allergic disorder or allergic disease.

In such methods, uses and medicaments, a peptide or protein can be derived from or based upon the allergen or can be derived from or based upon an allergen originating from the same organism as the allergen. More particularly, for example, a protein or peptide can be derived from or based upon an allergen causing the allergic reaction, allergic response, allergic disorder or allergic disease or said peptide derives from an allergen belonging to the same organism as the allergen causing said allergic reaction, allergic response, allergic disorder or allergic disease. Additionally, for example, a protein or peptide can be based upon or derived from an amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), or a variant or derivative thereof.

In various embodiments, a method or use desensitizes or induces, elicits, increases or improves immunological tolerance of a subject to an allergen of an organism or product of an organism in Table 1, Table 2, Table 3 or Table 4. In various other embodiments, a method or use desensitizes or induces, elicits, increases or improves immunological tolerance of a subject to an amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), or a variant or derivative thereof.

As set forth herein a protein, peptide, method, use or medicament can include administration or delivery by any means, systemically, regionally or locally. In particular aspects, a protein or peptide is administered cutaneously, subcutaneously, epicutaneously, intracutaneously, intramuscularly, intravenously, orally, mucosally, by inhalation or nasally. As also set forth herein a protein, peptide, method, use or medicament can include repeatedly contacting a cell with, or administering to a subject, the protein or peptide, multiple times.

Proteins and peptides can be used in diagnostic and detection methods and uses. In one embodiment, detecting an allergic response, or diagnosing an allergy in a subject, a method or use includes contacting a cell from the subject (which may be an ex vivo or in vivo cell) with a protein or peptide as set forth herein; and determining if the protein or peptide modulates an immune response or activity from the contacted cell. If the protein or peptide modulates an immune response or activity from the contacted cell (which may be an ex vivo or in vivo cell) detects an allergic response or indicates that the subject has an allergic response or an allergy. In particular aspects, modulation of immune response or activity is determined by assaying for a hypersensitive reaction or response, such as a cutaneous immunological hypersensitive reaction.

Subjects in accordance with invention include mammals, such as humans. In particular embodiments, a subject has exhibited a symptom of, or suffers from, an allergic reaction, allergic response, allergic disorder or allergic disease. In more particular embodiments, a subject has had an allergic reaction or allergic response to an allergen derived from or produced by Alder, Alternaria rot fungus, American cockroach, Dust mite, Ash, Aspergillus fumigatus, Bermuda grass, Birch, Black Walnut, Canary grass, Cat, Cladosporium herbarum, Common cypress, Cypress, Palm, Dog, English plantain, Giant ragweed, Japanese cypress, Kentucky blue grass, Lolium perenne, Mugwort weed, Orchard grass, Penicillium chrysogenum, Prickly juniper, Russian thistle, Rye grass, Sweet vernal grass, Western Ragweed or White oak. In further particular embodiments, a subject has had an allergic reaction or allergic response to an amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), or a variant or derivative thereof; or has had an allergic reaction or allergic response to a protein or peptide as set forth herein.

DESCRIPTION OF THE DRAWINGS

FIG. 1 shows that IFN-γ and IL-5 responses are optimally detected on day 14. IFN-γ and IL-5 responses to Rye Grass or Alternaria extract, in three correspondingly allergic donors each, were measured in ELISPOT assays on days 0, 3, 6, 14 and 17. The doses of individual extracts used for in vitro stimulation were chosen on the basis of initial dose titration studies, and were as shown in Table 6.

FIG. 2 shows a comparison of Alternaria epitopes identified on days 6 and 14 in two representative donors (D00095 and D00107). IL-5 or IFN-γ ELISPOT assays were performed, and positive responses defined, as described in Material and Methods. In terms of IL-5 responses in donor D00095, only peptide 14 was recognized on day 6; by day 14, peptides 11, 13, 14, 15, 16 and 17 were all positive. Similarly, in donor D00107 peptides 14 and 66 gave positive responses on days 6 and 14, while peptides 11, 12, 13, 15, 16, and 17 only gave responses on day 14. For IFN-γ, in both donors positive responses were only seen on day 14.

FIG. 3 shows a comparison of allergen epitope induced IL-4, -5 and -13 responses from cells in representative donors in response to Alternaria and Rye Grass peptides. Responses to IL-4, -5 and -13 were measured on day 14, as described herein. In donor U00064 Alternaria peptides 11, 13, 14, 15, 16, and 17 induced IL-5, IL-4 and IL-13 responses. Additionally, IL-5 responses were induced by peptides 12, 66, 67, 68 and 70. The data show that all epitopes inducing IL-4 and IL-13 responses also induced IL-5 responses. A similar pattern was noted in donor U00099 in response to Rye Grass peptides, where peptides 1168, 1175, 1181 and 1184 all induced IL-5 responses, and the same peptides encompassed all IL-4 and IL-13 responses.

FIG. 4 shows promiscuous HLA binding capacity of dominant epitopes. Individual 15-mer epitopes (n=96) from the 74 epitope regions derived from the 16 allergen sources where peptide specific responses could account for 15% or more of the responses detected with the extract, and that were recognized in two or more donors, were tested for their capacity to bind a panel of 35 different DR, DP and DQ molecules. The cumulative percentage of epitopes (black line) binding various fractions of the class II panel is shown. 73% of the epitopes bound 50% or more of the molecules tested with an affinity of 1000 nM, or better. Also shown is the cumulative percentage of peptides in a control panel of 425 unbiased, non-redundant, peptides binding various fractions of the same class II panel; only 17% of the control peptides bound 50% or more of the molecules tested.

FIG. 5 shows T cell responses to different allergen sources are differentially polarized. Percentage of the total response to the various allergen sources attributable to IL-5 or IFN-γ, representative of Th2 and Th1 responses, respectively, are shown as a bar graph. Median whealsize (mm in diameter) measured in correspondingly allergic donors are plotted on the secondary axis.

FIG. 6 shows polarized T cell responses to different allergens within an individual donor. IFNγ (black bars) and IL-5 (gray bars) responses, associated with Th1 and Th2 polarization, respectively, in donor D00069. As described, the T cell response, in terms of total SFC, to Aln g was associated only with only IFN-γ production, while the response to Alt a was only mediated by IL-5, highlighting the polarization of the responses to epitopes derived from Alder (Aln g) and Alternaria Rot Fungus (Alt a).

DETAILED DESCRIPTION

Thus, in accordance with the invention, there are provided proteins and peptides, or a subsequence, portion, homologue, variant or derivative thereof. In particular embodiments, proteins and peptides, subsequences, portions, homologues, variants or derivatives thereof are derived from or are based upon the amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). Invention proteins and peptides, subsequences, portions, homologues, variants or derivatives thereof are useful for among other things in the methods and uses described herein.

In particular embodiments, the peptide consist of or consist essentially of an amino acid sequence of Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance), Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), or a variant thereof or derivative thereof. The variant or derivative may be a peptide of up to 30 amino acids in length which comprises said amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance), Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In other particular aspects, a variant is a peptide from 7 to 30 amino acids in length, wherein at least 7 contiguous amino acids have at least 75% identity, such as at least 80% or 85% identity, to at least 7 contiguous amino acids of said amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance), Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance).

In still other embodiments, said variant peptide is up to 25 amino acids in length, such as up to 24, 23, 22, 21, 20, 19 or 19 amino acids in length. In particularly, said variant peptide is a peptide of 7 to 25 amino acids in length, such as 7 to 20; 8 to 30; 8 to 25; 8 to 20; 9 to 30; 9 to 25; 9 to 20; 10 to 30; 10 to 25; 10 to 20 amino acids in length and, wherein at least 7 contiguous amino acids have at least 75% identity, such as at least 80% or 85% identity, to at least 7 contiguous amino acids of said amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance), Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In other embodiments, the variant peptide has at least 8, 9 or 10 amino acids having at least 75% (such as at least 80% or 85%) identity or homology to at least 8, 9, or 10 contiguous amino acids, respectively of said corresponding amino acid sequence.

In particular embodiments, a protein or peptide comprises, consists of or consists essentially of an amino acid sequence of Der f 10 (SEQ ID NOs:568-570), Der f 11 (SEQ ID NO:1571), Der f 13 (SEQ ID NOs:1572-1575), Der f 14 (SEQ ID NO:1576), Der f 16 (SEQ ID NOs:1577-1584), Der f 18 (SEQ ID NOs:1585-1586), Der p 3 (SEQ ID NOs:1609-1614), Der p 4 (SEQ ID NOs:1615-1646), Der p 5 (SEQ ID NO:647), Der p 9 (SEQ ID NOs:1648-1650) or Der p 14 (SEQ ID NOs:1604-1607) as specified in Table 7.

In still other particular embodiments, a protein or peptide does not consist of the sequence of the peptide does not consist of the sequence of Alt a 6 (SEQ ID NO:1455, Asp f 1 (SEQ ID NO:1466), Fel d 1 1 (SEQ ID NOs:1531, 1532, 1533, 1534, 1535 1536, 1537, 1538), Fel d 12 (SEQ ID NOs:1539, 1540, 1541, 1542, 1543, 1544, 1545, 1546, 1547, 1548, 1549, 1550, 1551), Can f 3 (SEQ ID NOs:1557), Der f 1 (SEQ ID NOs:1562, 1563, 1564, 1565), Der f 2 (SEQ ID NO:1587), Der p1 (SEQ ID NOs:1591, 1593, 1594, 1595, 1596, 1597, 1598, 1599, 1600. 1601, 1602, 1603), Der p 2 (SEQ ID NO:1608), Aln g 1 (SEQ ID NOs:1653, 1654, 1655), Bet v 1 (SEQ ID NOs:1656, 1657, 1658, 1659, 1660, 1661, 1662, 1663, 1664), Bet v 2 (SEQ ID NO:1665), Cha o 1 (SEQ ID NOs:1678, 1679, 1680, 1681, 1682, 1683, 1684), Cup a 1 (SEQ ID NOs.: 1689, 1691, 1692), Cup s 1 (SEQ ID NOs.: 1700, 1701), Jun o 4 (SEQ ID NO:1710), Ant o 1 (SEQ ID NO:1743), Lol p 1 (SEQ ID NOs:1765, 1766, 1767, 1768), Lol p 11 (SEQ ID NO:1770), Lol p 5 1 (SEQ ID NOs:1793, 1794, 1795, 1796, 1797, 1798, 1802, 1803, 1804, 1805, 1807, 1808, 1809, 1811, 1812), Lol p 5 2 (SEQ ID NOs:1815, 1816, 1817, 1818, 1819), Pha a 1 (SEQ ID NO:1824), Pha a 5 (SEQ ID NOs:1831, 1833, 1836), Poa p 1 (SEQ ID NOs:1842, 1844), Poa p 5 (SEQ ID NOs:1845, 1846, 1847, 1848, 1849, 1850, 1851, 1852, 1853, 1854, 1855, 1856, 1859, 1860, 1861, 1862, 1863, 1864, 1865, 1866, 1867, 1868, 1869, 1870) or Amb t 5 (SEQ ID NO:1881) as set forth in Table 7.

As used herein, an “antigen” refers to a substance, including but not limited to a protein or peptide that elicits, induces, stimulates, promotes or enhances an immune response when administered to a subject. An immune response elicited by an antigen may include, but is not limited to, a B cell or a T cell response. An immune response can include a cellular response with a particular pattern of lymphokine/cytokine production (e.g., Th1, Th2), a humoral response (e.g., antibody production), or a combination thereof, to a particular antigen. For example, if a subject previously exposed to an allergen (i.e., is sensitized or is hypersensitive) comes into contact with the allergen again, allergic asthma may develop due to a Th2 response characterized by an increased production of type 2 cytokines (e.g., IL-4, IL-5, IL-9, and/or IL-13) secreted by CD4+ T lymphocytes.

As used herein an “epitope” refers to a region or part of an antigen that elicits an immune response when administered to a subject. In particular embodiments, an epitope may be comprised of a region or part of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In particular aspects, an epitope is a T cell epitope, i.e., an epitope that elicits, stimulates, induces, promotes, increases or enhances a T cell activity, function or response.

An antigen, epitope, allergen, or composition thereof can modulate an undesired or abnormal inflammatory response. An antigen, epitope, allergen, or composition thereof as described herein may alter the Th2 response by, for example, shifting the immune response toward a Th1 phenotype that is less damaging. That is, an altered (or modulated) immune response can decrease, inhibit, suppress, or reduce sensitivity (desensitize) to an antigen, epitope, or allergen, or against inflammatory responses (e.g., allergy, asthma, rash, wheezing, coughing, eye irritation, etc.) caused by an antigen, epitope, or allergen.

Accordingly, non-limiting examples of antigens, allergens are peptides and proteins having defined amino acid sequences and which comprise T cell epitopes, i.e., elicit, stimulate, induce, promote, increase or enhance a T cell response or activity. Antigens and allergens can be analyzed to determine whether they include at least one T cell epitope using any number of assays (e.g. T cell proliferation assays, lymphokine secretion assays, T cell non-responsiveness studies, etc.).

The term “allergen” refers to an antigen which elicits, induces, stimulates, or enhances an immune response by a cell or the immune system of an exposed animal (e.g., human). An antigen is an allergen when the specific immune response is the development of enhanced sensitivity or a hypersensitivity to the antigen, but the antigen itself is not typically innately harmful. An allergen is therefore a particular type of antigen that can cause development of enhanced or increased sensitivity or hypersensitivity in a subject. For example, an allergen can elicit production of IgE antibodies in predisposed subjects. Other examples of responses elicited by allergens include T cell responses or activity, such as production of a lymphokine, cytokine, or effector function on other cells. Responses caused by allergens are also described, for example, in Mol. Biol. of Allergy and Immunology, ed. R. Bush, Immunology and Allergy Clinics of North American Series (August 1996). Although the terms “allergen” and “antigen” have a different meaning, reference to “allergen” herein includes reference to “antigen” and reference to “antigen” herein includes reference to “allergen.”

Typically, allergens are organic substances, such as proteins, peptides, nucleotides, carbohydrates, lipids, fats, nucleic acid, and combinations or mixtures thereof. Allergen(s) as used herein include, but are not limited to a specific allergen protein, mixture of allergen proteins, an extract of an allergen, chemically or genetically manufactured allergen, or any combination thereof. Therefore, an “allergen” refers to allergen from plants (trees, weeds, crops, flowers, turf, grasses, etc., or parts thereof such as seeds, nuts, bark, pollen, leaves, etc.), or living or dead organisms (animals, bacterium, insects, mold, fungi, etc.) or products of organisms.

In certain embodiments, the proteins, peptides, subsequences, portions, homologues, variants and derivatives thereof, described herein stimulate, induce, promote, increase or enhance an immune response (for example, all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). In particular embodiments, a protein or peptide is a T cell antigen, allergen or epitope. In further particular embodiments, a protein or peptide, or a subsequence, portion, homologue, variant or derivative thereof, elicits, stimulates, promotes, induces or enhances a T cell response, which may include but is not limited to a Th2 cell response.

As used herein, the term “immune response” includes T cell (cellular) mediated and/or B cell (humoral) mediated immune responses. Exemplary immune responses include T cell responses, e.g., lymphokine production, cytokine production and cellular cytotoxicity. T-cell responses include Th1 and/or Th2 responses. In addition, the term immune response includes responses that are indirectly effected by T cell activation, e.g., antibody production (humoral responses) and activation of cytokine responsive cells, e.g., eosinophils, macrophages. Immune cells involved in the immune response include lymphocytes, such as T cells (CD4+, CD8+, Th1 and Th2 cells) and B cells; antigen presenting cells (e.g., professional antigen presenting cells such as dendritic cells, macrophages, B lymphocytes, Langerhans cells, and non-professional antigen presenting cells such as keratinocytes, endothelial cells, astrocytes, fibroblasts, oligodendrocytes); natural killer (NK) cells; myeloid cells, such as macrophages, eosinophils, mast cells, basophils, and granulocytes.

As set forth herein, a particular immunoglobulin (Ig) isotype may be produced in response to an antigen (allergen). For example, an “IgG antigen” refers to an antigen that induces an IgG antibody response. Likewise, an “IgE antigen” refers to an antigen that induces an IgE antibody response; an “IgA antigen” refers to an antigen that induces an IgA antibody response, and so forth. In certain embodiments, such an immunoglobulin (Ig) isotype produced in response to an antigen may also elicit production of other isotypes. For example, an IgG antigen may induce an IgG antibody response in combination with one more of an IgE, IgA, IgM or IgD antibody response. Accordingly, in certain embodiments, an IgG antigen may induce an IgG antibody response without inducing an IgE, IgA, IgM or IgD antibody response.

The invention encompasses methods and uses for reducing, decreasing, preventing the development of sensitization or hypersensitization to an antigen(s) or allergen(s). Accordingly, in other embodiments, a protein or peptide, subsequence, portion, homologue, variant or derivative thereof, decreases, inhibits, suppresses or reduces a T cell response, which may include but is not limited to a Th2 cell response. In certain embodiments, the T cell response is an anti-allergen immune response.

In accordance with another aspect of the invention there are provided a protein or peptide, a subsequence, portion, homologue, variant or derivative thereof, wherein the protein or peptide elicits, stimulates, induces, promotes, increases or enhances an anti-allergen immune response (for example, all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). In another aspect, there are provided a protein or peptide, subsequence, portion, homologue, variant or derivative thereof, wherein the protein or peptide decreases, reduces, inhibits, suppresses or disrupts an anti-allergen immune response.

As will be understood by a person of skill in the art, a protein or a subsequence, portion, homologue, variant or derivative thereof as described herein, may elicit, stimulate, induce, promote, increase or enhance certain elements of an anti-allergen immune response while decreasing, reducing, inhibiting, suppressing or reducing other elements of the anti-allergen response, either contemporaneously or sequentially. In one non-limiting example, a protein or a subsequence, portion, homologue, variant or derivative thereof may elicit, stimulate, induce, promote, increase or enhance proliferation of regulatory T cells while decreasing, reducing, inhibiting, suppressing or reducing production of proinflammatory lymphokines/cytokines.

An “anti-allergen,” “anti-protein,” or “anti-peptide immune response” refers to an immune response that is particular or specific for the protein or peptide, e.g., allergen. In such instances, the response is specifically triggered (elicited, stimulated, increased, induced, or promoted) by the protein or peptide, e.g., allergen. Although “anti-allergen” immune response is specifically triggered by a given allergen, the immune response itself can be characterized by general features of immune responses, such as T cell (cellular) and/or B cell (humoral) immune responses, as set forth herein.

As disclosed herein, a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, may elicit, stimulate, induce, promote, increase or enhance immunological tolerance to an antigen, including an allergen. In certain embodiments, a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, described herein may elicit, stimulate, induce, promote, increase or enhance immunological tolerance to an allergen. Thus in certain embodiments a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, described herein may be effective in use or treatment (e.g., therapeutic) of an allergic reaction or allergic immune response, including but not limited to an allergic response following a secondary or subsequent exposure of a subject to an antigen or allergen. In particular embodiments, immunological tolerance elicited, stimulated, induced, promoted, increased or enhanced from use or administration of the a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, may involve modulation of T cell activity, including but not limited to CD4+ T cells, CD8+ T cells, Th1 cells, Th2 cells and regulatory T cells (Tregs). For example, immunological tolerance elicited, stimulated, induced, promoted, increased or enhanced from use or administration of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, may involve modulation of the production or activity of pro-inflammatory or anti-inflammatory lymphokines/cytokines produced by T cells. Thus, in accordance with certain aspects of the invention, there are provided proteins, peptides, subsequences, portions, homologues, variants and derivatives thereof, that elicits, stimulates, induces, promotes, increases or enhances immunological tolerance to an antigen or allergen.

An allergic reaction refers to a local or general reaction in a subject following contact with a specific antigen (e.g., allergen) to which the subject had been previously exposed and had become sensitized. The immunologic interaction of antigen (e.g., allergen) with sensitized lymphocytes (T cells) and/or antibody can give rise to inflammation and tissue damage. An allergy is an undesirable immune response or reaction that can therefore produce damage to self-tissues and cells, usually through inflammatory reactions.

One non-limiting example of an allergy is asthma. Asthma, which can be extrinsic or allergic asthma (also referred to as reactive airway disease), is an inflammatory disease of the lungs characterized by a generally reversible airway obstruction. Non-limiting features of allergic asthma include elevated concentrations of serum IgE, pulmonary eosinophilia, airway hyper-responsiveness, excessive airway mucus production, and airway remodeling marked by peribronchiolar collagen deposition and increases in airway smooth muscle mass. Other exemplary allergic reactions or inflammatory conditions include allergic alveolitis, allergic bronchopulmonary aspergillosis, allergic dermatitis, eczema, allergic conjunctivitis, allergic coryza, allergic vasculitis, rhinosinusitis, and allergic rhinitis.

Hypersensitivity or hyper-responsiveness used in reference to an immune response means an abnormal response or condition in which an antigen or allergen elicits an exaggerated immune response. For example, allergic asthma can result from repeated exposure to airborne allergens that trigger detrimental immunological responses, such as persistent inflammation in the bronchial wall, which can in turn cause structural and functional changes in the respiratory system. After allergen contact by sensitized subjects (i.e., those subjects that have already been exposed to the allergen), the immune response is dependent on CD4+ T lymphocytes that are skewed to a T helper (Th) 2 phenotype. Th2 cytokines, for example, IL-4, IL-5, IL-9, and IL-13 are produced and are believed to contribute to asthma pathogenesis. For example, IL-4 drives the T helper response in favor of Th2, resulting in enhanced production of IgE; IL-5, which with granulocyte macrophage colony stimulating factor (GM-CSF) and IL-3, is important for the production of eosinophils; and IL-13, which is required for airway hyper-responsiveness and mucous metaplasia, which are downstream pathophysiological features that are closely linked with clinical asthma. All of these cytokines, together with TGF-beta have been implicated in airway remodeling. Increased numbers of airway eosinophils is also associated with disease severity, although the role of eosinophils in the pathology of asthma is not entirely understood, (see, e.g., Lee et al., Science 305:1773 (2004); Humbles et al., Science 305:1776 (2004)). The resulting structural and morphometric changes (remodeling) include subepithelial fibrosis, goblet cell hyperplasia and metaplasia, which result in functional consequences such as loss of distensibility of asthmatic airways, bronchial hyper-reactivity (even in the absence of the allergen), and an accelerated progressive decrease in forced expiratory volume at 1 second time intervals. Th2 cytokines may also prime and activate eosinophils to release proinflammatory agents, lipid mediators, and other cytokines thought to contribute to the observed tissue damage, remodeling, and hyper-responsiveness.

As used herein, the term “tolerance,” “anergy,” or “antigen (allergen)-specific tolerance” refers to a reduction, loss, inhibition, suppression or decrease, of T cells to T cell receptor-mediated stimulation by an allergen or antigen. The reduction can lead to reduced or non-responsiveness (insensitivity) of T cells to an allergen or antigen. Such insensitivity is generally antigen-specific and persists after exposure to the antigenic peptide has ceased. For example, tolerance in T cells is characterized by lack of lymphokine/cytokine production, e.g., IL-2, IFN-γ, or TNF-β. T-cell anergy occurs when T cells are exposed to antigen or allergen and receive a first signal (a T cell receptor or CD-3 mediated signal) in the absence of a second signal (a costimulatory signal). Under these conditions, re-exposure of the cells to the same antigen or allergen (even if re-exposure occurs in the presence of a costimulatory molecule) results in failure to produce cytokines and subsequently failure of T cells to proliferate. Thus, a failure to produce lymphokines/cytokines prevents proliferation. Tolerized T cells can, however, proliferate if cultured with cytokines (e.g., IL-2). For example, T cell anergy can also be observed by the lack of IL-2 production by T lymphocytes as measured by ELISA or by a proliferation assay using an indicator cell line.

As used herein, the term “immunological tolerance” refers to a) a decreased or reduced level of a specific immunological response (thought to be mediated at least in part by antigen-specific effector T lymphocytes, B lymphocytes, antibody, a combination); b) a delay in the onset or progression of a specific immunological response; or c) a reduced risk of the onset or progression of a specific immunological response to an antigen or allergen. “Specific” immunological tolerance occurs when tolerance is preferentially invoked against certain antigens (allergens) in comparison with other antigens (allergens). Tolerance is an active antigen dependent process and differs from non-specific immunosuppression and immunodeficiency.

An increase, improvement, enhancement or induction of “tolerance” refers to a decrease, reduction, inhibition, suppression, or limiting or controlling or clearing of specific immunological reactivity to an antigen as compared to reactivity to the antigen in a previous exposure to the same antigen. Thus in certain embodiments, a method or use of inducing immunological tolerance in a subject to an allergen includes elimination of an allergic response of the subject to the allergen. Immunological tolerance in a subject to an allergen can also be reflected by reducing the occurrence, frequency, severity, progression, or duration of an allergic response of the subject to the antigen or allergen.

While desirably tolerance can refer to non-reactivity to an antigen or allergen, tolerance need not be complete non-reactivity and can only be partial, and in any event is reflected by a decrease, inhibition, suppression or reduction in specific immunological reactivity to an antigen or allergen as compared to reactivity to the antigen or allergen in a previous exposure to the same antigen or allergen (or epitope thereof). Thus, in another embodiment, a method or use of inducing immunological tolerance in a subject to an allergen includes stabilizing or maintaining the level of an allergic response in the subject to the allergen.

Induction of immune tolerance (also referred to as desensitization), and the relative amount of immune tolerance, can be measured by methods disclosed herein or known to the skilled artisan. For example, induction of immune tolerance can be measured by modulation of lymphokine and/or cytokine level in said animal. As such, modulation can be an increase of a cytokine level, for instance an increase of a cytokine level at least 1.5, 2, 3 times or more relative to before said induction. Alternatively, modulation can be a decrease of the level of a particular cytokine level, for instance a decrease of the cytokine level is at least 1.5, 2, 3 times or more relative to before said induction. The lymphokines/cytokines chosen to measure can be from any relevant lymphokines/cytokines, such as IL-2, IL-5, IL-4, IL-6, IL-10, IL-12, IL-13, TNF-α, IFN-γ, IFN-α, TGF-β, MCP-1, RANK-L and Flt3L.

As disclosed herein, peptides and proteins of the invention are useful in methods and uses, for example, of “specific” immunotherapy. The term “specific” immunotherapy refers to a therapy particular or specific for the protein or peptide, e.g., allergen. To achieve “specific immunotherapy” an antigen is administered to a subject in order to achieve immunological tolerance of the subject to an antigen, including for example, an allergen. More particularly, specific immunotherapy may be conducted by administering an antigen derived from the antigen (e.g. allergen) against which immunological tolerance is sought. Alternatively, immunotherapy can be conducted by “non”-specific immunotherapy using a different antigen or protein than the antigen against which immunological tolerance is sought. Thus, in different embodiments, the antigen administered and antigen (e.g. allergen) against which immunological tolerance is sought may be the same protein, may be proteins of or produce by the same organism, or may be proteins of different organisms. In various embodiments, a method or use of specific immunotherapy reduces, inhibits, suppresses or decreases sensitivity or (hyper)sensitivity to the protein or peptide, e.g., allergen, or elicits, stimulates, increases, induces, promotes or improves tolerance of the protein or peptide, e.g., allergen. Typically a subject is administered a protein or peptide, e.g., allergen, for example, via a subcutaneous injection.

Methods and uses include multi-does regimens. For example, a method or use can begin with small doses of allergen, and the doses are increased for repeated contact or administration.

A variant or derivative of an antigen, including an allergen as described herein, or a subsequence or portion of an antigen or allergen, include molecules that are structurally similar and functionally similar (e.g., for example to any sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). A variant, derivative or subsequence of antigen or allergen is functionally similar to the antigen or allergen sequence if the variant, derivative or subsequence is capable of eliciting a detectable or measurable immune response, even if it is a reduced immune response compared to the nonvariant/non-derived or native sequence, which may be determined using methods, including animal models and in vitro assays, described herein and know to one of skill in the art. For example, an immune response may be determined by quantitative and/or qualitative determination of lymphokine/cytokine production (e.g., by T cells), antibody production (including class and/or isotype), cellular mobilization, migration or motility, and optionally in vivo, such as an animal model of antigen/allergen immune responsiveness. An immune response of variant, derivative or subsequence of antigen or allergen compared to the non-variant/non-derivatized/native full length antigen or allergen may be ascertained by analysis of a particular measure (such as lymphokine/cytokine production, immunoglobulin production, cell mobilization, migration, motility, etc.) and may be greater, less than or comparable, e.g., within 5%, 10%, 15%, or 20% or 25% of the immune response of non-variant/non-derivatized/native full length antigen or allergen. For example, levels of Th1 lymphokines/cytokines, such as IFN-γ, IL-2, and TNF-β and Th2 cytokines, such as IL-4, IL-5, IL-9, IL-10, and IL-13, may be determined according to methods described herein or known to one of skill in the art.

As disclosed herein, proteins and peptides, or a subsequence, portion, homologue, variant or derivative thereof include those having all or at least partial sequence identity to one or more exemplary proteins and peptides, or a subsequence, portion, homologue, variant or derivative thereof (e.g., sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). The term “identity” and “identical” and grammatical variations thereof, mean that two or more referenced entities are the same (e.g., peptides or polynucleotide molecules). Thus, where two proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof are identical, they have the same amino acid sequence. The identity can be over a defined area (region or domain) of the sequence. “Areas, regions or domains” of homology or identity mean that a portion of two or more referenced entities share homology or are the same.

Invention proteins and peptides exclude certain proteins and peptides set forth in Table 7 as they are identical to proteins and peptides known in the art. In particular embodiments, at least proteins and peptides with sequences identical to the sequences of Alt a 6 (SEQ ID NO:1455, Asp f 1 (SEQ ID NO:1466), Fel d 1 1 (SEQ ID NOs:1531, 1532, 1533, 1534, 1535 1536, 1537, 1538), Fel d 12 (SEQ ID NOs:1539, 1540, 1541, 1542, 1543, 1544, 1545, 1546, 1547, 1548, 1549, 1550, 1551), Can f 3 (SEQ ID NOs:1557), Der f 1 (SEQ ID NOs:1562, 1563, 1564, 1565), Der f 2 (SEQ ID NO:1587), Der p1 (SEQ ID NOs:1591, 1593, 1594, 1595, 1596, 1597, 1598, 1599, 1600. 1601, 1602, 1603), Der p 2 (SEQ ID NO:1608), Aln g 1 (SEQ ID NOs:1653, 1654, 1655), Bet v 1 (SEQ ID NOs:1656, 1657, 1658, 1659, 1660, 1661, 1662, 1663, 1664), Bet v 2 (SEQ ID NO:1665), Cha o 1 (SEQ ID NOs:1678, 1679, 1680, 1681, 1682, 1683, 1684), Cup a 1 (SEQ ID NOs:1689, 1691, 1692), Cup s 1 (SEQ ID NOs:1700, 1701), Jun o 4 (SEQ ID NO:1710), Ant o 1 (SEQ ID NO:1743), Lol p 1 (SEQ ID NOs:1765, 1766, 1767, 1768), Lol p 11 (SEQ ID NO:1770), Lol p 5 1 (SEQ ID NOs:1793, 1794, 1795, 1796, 1797, 1798, 1802, 1803, 1804, 1805, 1807, 1808, 1809, 1811, 1812), Lol p 5 2 (SEQ ID NOs:1815, 1816, 1817, 1818, 1819), Pha a 1 (SEQ ID NO:1824), Pha a 5 (SEQ ID NOs:1831, 1833, 1836), Poa p 1 (SEQ ID NOs:1842, 1844), Poa p 5 (SEQ ID NOs:1845, 1846, 1847, 1848, 1849, 1850, 1851, 1852, 1853, 1854, 1855, 1856, 1859, 1860, 1861, 1862, 1863, 1864, 1865, 1866, 1867, 1868, 1869, 1870) or Amb t 5 (SEQ ID NO:1881) as set forth in Table 7. In the event that proteins and peptides are excluded on the basis of identity to sequences known in the art, such as at least sequences of at least proteins and peptides with sequences identical to the sequences of Alt a 6 (SEQ ID NO:1455, Asp f 1 (SEQ ID NO:1466), Fel d 1 1 (SEQ ID NOs:1531, 1532, 1533, 1534, 1535 1536, 1537, 1538), Fel d 12 (SEQ ID NOs:1539, 1540, 1541, 1542, 1543, 1544, 1545, 1546, 1547, 1548, 1549, 1550, 1551), Can f 3 (SEQ ID NOs:1557), Der f 1 (SEQ ID NOs:1562, 1563, 1564, 1565), Der f 2 (SEQ ID NO:1587), Der p1 (SEQ ID NOs:1591, 1593, 1594, 1595, 1596, 1597, 1598, 1599, 1600. 1601, 1602, 1603), Der p 2 (SEQ ID NO:1608), Aln g 1 (SEQ ID NOs:1653, 1654, 1655), Bet v 1 (SEQ ID NOs:1656, 1657, 1658, 1659, 1660, 1661, 1662, 1663, 1664), Bet v 2 (SEQ ID NO:1665), Cha o 1 (SEQ ID NOs:1678, 1679, 1680, 1681, 1682, 1683, 1684), Cup a 1 (SEQ ID NOs:1689, 1691, 1692), Cup s 1 (SEQ ID NOs:1700, 1701), Jun o 4 (SEQ ID NO:1710), Ant o 1 (SEQ ID NO:1743), Lol p 1 (SEQ ID NOs:1765, 1766, 1767, 1768), Lol p 11 (SEQ ID NO:1770), Lol p 5 1 (SEQ ID NOs:1793, 1794, 1795, 1796, 1797, 1798, 1802, 1803, 1804, 1805, 1807, 1808, 1809, 1811, 1812), Lol p 5 2 (SEQ ID NOs:1815, 1816, 1817, 1818, 1819), Pha a 1 (SEQ ID NO:1824), Pha a 5 (SEQ ID NOs:1831, 1833, 1836), Poa p 1 (SEQ ID NOs:1842, 1844), Poa p 5 (SEQ ID NOs:1845, 1846, 1847, 1848, 1849, 1850, 1851, 1852, 1853, 1854, 1855, 1856, 1859, 1860, 1861, 1862, 1863, 1864, 1865, 1866, 1867, 1868, 1869, 1870) or Amb t 5 (SEQ ID NO:1881) as set forth in Table 7, subsequences, portions, variants and derivatives thereof as set forth herein are not excluded. Furthermore, such proteins and peptides such as sequences of Alt a 6 (SEQ ID NO:1455, Asp f 1 (SEQ ID NO:1466), Fel d 1 1 (SEQ ID NOs:1531, 1532, 1533, 1534, 1535 1536, 1537, 1538), Fel d 12 (SEQ ID NOs:1539, 1540, 1541, 1542, 1543, 1544, 1545, 1546, 1547, 1548, 1549, 1550, 1551), Can f 3 (SEQ ID NOs:1557), Der f 1 (SEQ ID NOs:1562, 1563, 1564, 1565), Der f 2 (SEQ ID NO:1587), Der p1 (SEQ ID NOs:1591, 1593, 1594, 1595, 1596, 1597, 1598, 1599, 1600. 1601, 1602, 1603), Der p 2 (SEQ ID NO:1608), Aln g 1 (SEQ ID NOs:1653, 1654, 1655), Bet v 1 (SEQ ID NOs:1656, 1657, 1658, 1659, 1660, 1661, 1662, 1663, 1664), Bet v 2 (SEQ ID NO:1665), Cha o 1 (SEQ ID NOs:1678, 1679, 1680, 1681, 1682, 1683, 1684), Cup a 1 (SEQ ID NOs:1689, 1691, 1692), Cup s 1 (SEQ ID NOs:1700, 1701), Jun o 4 (SEQ ID NO:1710), Ant o 1 (SEQ ID NO:1743), Lol p 1 (SEQ ID NOs:1765, 1766, 1767, 1768), Lol p 11 (SEQ ID NO:1770), Lol p 5 1 (SEQ ID NOs:1793, 1794, 1795, 1796, 1797, 1798, 1802, 1803, 1804, 1805, 1807, 1808, 1809, 1811, 1812), Lol p 5 2 (SEQ ID NOs:1815, 1816, 1817, 1818, 1819), Pha a 1 (SEQ ID NO:1824), Pha a 5 (SEQ ID NOs:1831, 1833, 1836), Poa p 1 (SEQ ID NOs:1842, 1844), Poa p 5 (SEQ ID NOs:1845, 1846, 1847, 1848, 1849, 1850, 1851, 1852, 1853, 1854, 1855, 1856, 1859, 1860, 1861, 1862, 1863, 1864, 1865, 1866, 1867, 1868, 1869, 1870) or Amb t 5 (SEQ ID NO:1881) as set forth in Table 7 may also be optionally included in the methods and uses set forth herein.

Identity can be determined by comparing each position in aligned sequences. A degree of identity between amino acid sequences is a function of the number of identical or matching amino acids at positions shared by the sequences, i.e. over a specified region. Optimal alignment of sequences for comparisons of identity may be conducted using a variety of algorithms, as are known in the art, including the ClustalW program, available at http://clustalw.genome.ad.jp, the local homology algorithm of Smith and Waterman, 1981, Adv. Appl. Math 2: 482, the homology alignment algorithm of Needleman and Wunsch, 1970, J. Mol. Biol. 48:443, the search for similarity method of Pearson and Lipman, 1988, Proc. Natl. Acad. Sci. USA 85: 2444, and the computerized implementations of these algorithms (such as GAP, BESTFIT, FASTA and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, Madison, Wis., U.S.A.). Sequence identity may also be determined using the BLAST algorithm, described in Altschul et al., 1990, J. Mol. Biol. 215:403-10 (using the published default settings). Software for performing BLAST analysis may be available through the National Center for Biotechnology Information (through the internet at http://www.ncbi.nlm.nih.gov/). Such algorithms that calculate percent sequence identity (homology) generally account for sequence gaps and mismatches over the comparison region or area. For example, a BLAST (e.g., BLAST 2.0) search algorithm (see, e.g., Altschul et al., J. Mol. Biol. 215:403 (1990), publicly available through NCBI) has exemplary search parameters as follows: Mismatch −2; gap open 5; gap extension 2. For polypeptide sequence comparisons, a BLASTP algorithm is typically used in combination with a scoring matrix, such as PAM100, PAM 250, BLOSUM 62 or BLOSUM 50. FASTA (e.g., FASTA2 and FASTA3) and SSEARCH sequence comparison programs are also used to quantitate the extent of identity (Pearson et al., Proc. Natl. Acad. Sci. USA 85:2444 (1988); Pearson, Methods Mol Biol. 132:185 (2000); and Smith et al., J. Mol. Biol. 147:195 (1981)). Programs for quantitating protein structural similarity using Delaunay-based topological mapping have also been developed (Bostick et al., Biochem Biophys Res Commun. 304:320 (2003)).

As described herein, proteins and peptides include homologues (e.g., of any sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). A polypeptide sequence or polynucleotide sequence is a “homologue” of, or is “homologous” to, another sequence if the two sequences have substantial identity over a specified region and a functional activity of the sequences is preserved or conserved, at least in part (as used herein, the term ‘homologous’ does not infer evolutionary relatedness). Two polypeptide sequences or polynucleotide sequences are considered to have substantial identity if, when optimally aligned (with gaps permitted), they share at least about 40% sequence identity or greater (e.g. 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, etc.) identify over a specific region, for example, over all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008), or if the sequences share defined functional motifs (e.g., epitopes). The percent identity can extend over the entire sequence length or a portion of the sequence (for example, over all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). In particular aspects, the length of the sequence sharing the percent identity is 2, 3, 4, 5 or more contiguous amino acids, e.g., 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, etc. contiguous amino acids (for example, over all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). In additional particular aspects, the length of the sequence sharing the percent identity is 20 or more contiguous amino acids, e.g., 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, etc. contiguous amino acids (for example, over all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). In further particular aspects, the length of the sequence sharing the percent identity is 35 or more contiguous amino acids, e.g., 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 45, 47, 48, 49, 50, etc., contiguous amino acids (for example, over all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). In yet further particular aspects, the length of the sequence sharing the percent identity is 50 or more contiguous amino acids, e.g., 50-55, 55-60, 60-65, 65-70, 70-75, 75-80, 80-85, 85-90, 90-95, 95-100, etc. contiguous amino acids (for example, over all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008).

An “unrelated” or “non-homologous” sequence shares less than 30% identity. More particularly, shares less than about 25% identity, with a protein, peptide or polynucleotide of the invention over a specified region of homology.

A variant or derivative of a protein or peptide refers to a modified or variant form of the protein or peptide, or subsequence, portion or homologue thereof (for example, of all or a part of a sequence in any of Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). Such modified forms, such as amino acid deletions, additions and substitutions, of the proteins and peptides can also be used in the invention uses, methods and compositions, including methods for modulating an immune response, eliciting, stimulating, inducing, promoting, increasing, or enhancing immunological tolerance and protecting and treating subjects against an allergic reaction or response, as set forth herein.

Thus, in accordance with the invention, modified, variant and derivative forms of proteins, peptides, subsequences, portions, and homologues thereof are provided that have one or more functions or activities of unmodified, non-variant and non-derivatized forms of proteins and peptides. Such forms, referred to as “modifications”, “variants” or “derivatives” and grammatical variations thereof deviate from a reference sequence. For example, as described herein, a protein, peptide, subsequence, portion, or homologue thereof may comprise, consist or consist essentially of an amino acid sequence that is a modification, variant, or derivative of a protein or an amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). Such modifications, variants, or derivatives may have greater or less activity or function than a reference protein or peptide, such as ability to elicit, stimulate, induce, promote, increase, enhance, activate, modulate, inhibit, decreases, suppress, or reduce an immune response (e.g. a T cell response) or elicit, stimulate, induce, promote, increase or enhance immunological tolerance (desensitize) to an antigen or allergen. Thus, proteins, peptides, or subsequences, portions or homologues thereof include sequences having substantially the same, greater or less relative activity or function as a reference antigen or allergen (e.g., any of the proteins or peptides set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance) for example, an ability to elicit, stimulate, induce, promote, increase, enhance, activate, modulate, inhibit, suppress, decrease or reduce an immune response (e.g. a T cell response) or elicit, stimulate, induce, promote, increase or enhance immunological tolerance to an antigen or allergen in vitro or in vivo.

A variant or derivative therefore includes deletions, including truncations and fragments; insertions and additions, including tagged polypeptides and fusion proteins; substitutions, for example conservative substitutions, site-directed mutants and allelic variants; and modifications, including peptoids having one or more non-amino acyl groups (q.v., sugar, lipid, etc.) covalently linked to the peptide and post-translational modifications.

Non-limiting examples of modifications include one or more amino acid substitutions (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 20-25, 25-30, 30-50, 50-100, or more residues), additions and insertions (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 20-25, 25-30, 30-50, 50-100, or more residues) and deletions (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 20-25, 25-30, 30-50, 50-100) of a reference protein, peptide, or subsequence or portion thereof (e.g., of any of the proteins or peptides set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In particular embodiments, a modified or variant sequence retains at least part of a function or an activity of unmodified sequence, and can have less than, comparable, or greater, but at least a part of, a function or activity of a reference sequence, for example, the ability elicit, stimulate, induce, promote, increase, enhance, activate, modulate, inhibit, suppress, decrease, or reduce an immune response (e.g. a T cell response) or elicit, stimulate, induce, promote, increase or enhance immunological tolerance to an allergen. Such immune responses include, for example, among others, induced, increased, enhanced, stimulated, activated, modulated, inhibited, suppressed, decreased or reduced expression, production or activity of a cytokine (e.g., IL-5), an antibody (e.g. increase production of IgG antibodies, decrease production of IgE) or an immune cell (e.g. CD4+ T cell, CD8+ T cell, Th1 cell, Th2 cell or regulatory T cell).

Variants and derivatives of proteins and peptides include naturally-occurring polymorphisms or allelic variants, strain variants, as well as synthetic proteins and peptides that contain a limited number of conservative amino acid substitutions of the amino acid sequence. A variety of criteria can be used to indicate whether amino acids at a particular position in a protein or peptide are similar. In making such changes, substitutions of like amino acid residues can be made on the basis of relative similarity of side-chain substituents, for example, their size, charge, hydrophobicity, hydrophilicity, and the like, and such substitutions may be assayed for their effect on the function of the peptide by routine testing.

Specific non-limiting examples of substitutions include conservative and non-conservative amino acid substitutions. A “conservative substitution” is the replacement of one amino acid by a biologically, chemically or structurally similar residue. Biologically similar means that the substitution does not destroy a biological activity. Structurally similar means that the amino acids have side chains with similar length, such as alanine, glycine and serine, or a similar size. Chemical similarity means that the residues have the same charge, or are both hydrophilic or hydrophobic. For example, a conservative amino acid substitution is one in which an amino acid residue is replaced with an amino acid residue having a similar side chain, which include amino acids with basic side chains (e.g., lysine, arginine, histidine); acidic side chains (e.g., aspartic acid, glutamic acid); uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, histidine); nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan); beta-branched side chains (e.g., threonine, valine, isoleucine), and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan). Particular examples include the substitution of one hydrophobic residue, such as isoleucine, valine, leucine or methionine for another, or the substitution of one polar residue for another, such as the substitution of arginine for lysine, glutamic for aspartic acids, or glutamine for asparagine, serine for threonine, and the like. Proline, which is considered more difficult to classify, shares properties with amino acids that have aliphatic side chains (e.g., Leu, Val, Ile, and Ala). In certain circumstances, substitution of glutamine for glutamic acid or asparagine for aspartic acid may be considered a similar substitution in that glutamine and asparagine are amide derivatives of glutamic acid and aspartic acid, respectively. Conservative changes can also include the substitution of a chemically derivatized moiety for a non-derivatized residue, for example, by reaction of a functional side group of an amino acid. Variants and derivatives of proteins and peptides include forms having a limited number of one or more substituted residues.

An addition can be a covalent or non-covalent attachment of any type of molecule. Specific examples of additions include glycosylation, acetylation, phosphorylation, amidation, formylation, ubiquitination, and derivatization by protecting/blocking groups and any of numerous chemical modifications. Additional specific non-limiting examples of an addition are one or more additional amino acid residues. Accordingly, proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, can be a part of or contained within a larger molecule, such as another protein or peptide sequence, such as a fusion or chimera with a different (distinct) sequence.

In particular embodiments, an addition is a fusion (chimeric) sequence, an amino acid sequence having one or more molecules not normally present in a reference native (wild type) sequence covalently attached to the sequence. The term “chimeric” and grammatical variations thereof, when used in reference to a sequence, means that the sequence contains one or more portions that are derived from, obtained or isolated from, or based upon other physical or chemical entities. For example, a chimera of two or more different proteins may have one part a protein, peptide, subsequence, portion, homologue or variant thereof, and a second part of the chimera may be from a different sequence, or unrelated protein sequence.

Another particular example of a sequence having an amino acid addition is one in which a second heterologous sequence, i.e., heterologous functional domain is attached (covalent or non-covalent binding) that confers a distinct or complementary function. Heterologous functional domains are not restricted to amino acid residues. Thus, a heterologous functional domain can consist of any of a variety of different types of small or large functional moieties. Such moieties include nucleic acid, peptide, carbohydrate, lipid or small organic compounds, such as a drug (e.g., an antiviral), a metal (gold, silver), and radioisotope. For example, a tag such as T7 or polyhistidine can be attached in order to facilitate purification or detection of a protein, peptide, etc. Accordingly, there are provided proteins, peptides, subsequences, portions and homologues thereof, and a heterologous domain, wherein the heterologous functional domain confers a distinct function on the protein, peptide, subsequence, portion or homologue thereof.

Linkers, such as amino acid or peptidomimetic sequences may be inserted between the sequence and the addition (e.g., heterologous functional domain) so that the two entities maintain, at least in part, a distinct function or activity. Linkers may have one or more properties that include a flexible conformation, an inability to form an ordered secondary structure or a hydrophobic or charged character, which could promote or interact with either domain. Amino acids typically found in flexible protein regions include Gly, Asn and Ser. Other near neutral amino acids, such as Thr and Ala, may also be used in the linker sequence. The length of the linker sequence may vary without significantly affecting a function or activity of the fusion protein (see, e.g., U.S. Pat. No. 6,087,329). Linkers further include chemical moieties and conjugating agents, such as sulfo-succinimidyl derivatives (sulfo-SMCC, sulfo-SMPB), disuccinimidyl suberate (DSS), disuccinimidyl glutarate (DSG) and disuccinimidyl tartrate (DST).

Further non-limiting examples of additions are detectable labels. Thus, in another embodiment, the invention provides proteins, peptides, subsequences, portions and homologues thereof, that are detectably labeled. Specific examples of detectable labels include fluorophores, chromophores, radioactive isotopes (e.g., S³⁵, P³², I¹²⁵), electron-dense reagents, enzymes, ligands and receptors. Enzymes are typically detected by their activity. For example, horseradish peroxidase is usually detected by its ability to convert a substrate such as 3,3-′,5,5-′-tetramethylbenzidine (TMB) to a blue pigment, which can be quantified.

Another non-limiting example of an addition is an insertion of an amino acid within any protein, peptide, subsequence, portion or homologue thereof (e.g., any protein or sequence set forth herein, such as in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In particular embodiments, an insertion is of one or more amino acid residues inserted into the amino acid sequence of a protein or peptide, or subsequence, portion or homologue thereof, such as any protein or sequence set forth herein, such as in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance).

Modified and variant proteins, peptides, subsequences, portions or homologues thereof also include one or more D-amino acids substituted for L-amino acids (and mixtures thereof), structural and functional analogues, for example, peptidomimetics having synthetic or non-natural amino acids or amino acid analogues and derivatized forms. Modifications include cyclic structures such as an end-to-end amide bond between the amino and carboxy-terminus of the molecule or intra- or inter-molecular disulfide bond. Proteins, peptides, subsequences, portions and homologues thereof may be modified in vitro or in vivo, e.g., post-translationally modified to include, for example, sugar residues, phosphate groups, ubiquitin, fatty acids, lipids, etc.

Specific non-limiting examples of modified and variant proteins, peptides, subsequences, portions and homologues thereof include proteins or peptides comprising, consisting or consisting essentially of an amino acid sequence comprising at least one amino acid deletion from a full length protein or amino acid sequence set forth in any of Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In particular embodiments, a protein, peptide, or subsequence, portion or homologue thereof is from about 2 to up to one amino acid less than the full length protein sequence. In additional particular embodiments, a protein subsequence or portion is from about 2 to 5, 5 to 10, 10 to 15, 15 to 20, 20 to 25, 25 to 50, 50 to 100 amino acids in length, provided that said subsequence or portion is at least one amino acid less in length than the full-length protein sequence.

The term “subsequence” or “portion” means a fragment or part of the full length molecule. A subsequence or portion therefore consists of one or more amino acids less than the full length protein or peptide. A subsequence or portion of can have one or more amino acids less than the full length protein or peptide internally or terminal amino acid deletions from either amino or carboxy-termini. Subsequences and portions can vary in size. For example, a subsequence or portion of a protein or peptide can be as small as an epitope capable of binding an antibody (i.e., about five amino acids) up to a polypeptide that is one amino acid less than the entire length of a reference protein or peptide.

As used herein, subsequences and portions may also include or consist of one or more amino acid additions or deletions, wherein the subsequence or portion does not comprise the full length native/wild type protein or peptide sequence. Accordingly, total subsequence or portion lengths can be greater than the length of the full length native/wild type protein or peptide, for example, where a protein or peptide subsequence is fused or forms a chimera with another polypeptide.

The invention provides isolated and/or purified proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof. In particular embodiments, the isolated and/or purified proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, comprise, consist of or consist essentially of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In particular embodiments, the isolated and/or purified proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof include a T cell epitope.

The term “isolated,” when used as a modifier of a composition, means that the compositions are made by the hand of man or are separated, completely or at least in part, from their naturally occurring in vivo environment. Generally, isolated compositions are substantially free of one or more materials with which they normally associate with in nature, for example, one or more protein, nucleic acid, lipid, carbohydrate, cell membrane. The term “isolated” does not exclude alternative physical forms of the composition, such as fusions/chimeras, multimers/oligomers, modifications (e.g., phosphorylation, glycosylation, lipidation) or derivatized forms, or forms expressed in host cells produced by the hand of man.

An “isolated” composition (e.g., proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of any of Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) can also be “substantially pure” or “purified” when free of most or all of the materials with which it typically associates with in nature. Thus, an isolated protein, peptide, subsequence, portion, homologue, variant or derivative thereof, that also is substantially pure or purified does not include polypeptides or polynucleotides present among millions of other sequences, such as peptides of an peptide library or nucleic acids in a genomic or cDNA library, for example.

A “substantially pure” or “purified” composition can be combined with one or more other molecules. Thus, “substantially pure” or “purified” does not exclude combinations of compositions, such as combinations of proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof (e.g., multiple proteins, peptides, subsequences, etc.), and other antigens, agents, drugs or therapies.

Proteins and peptide (e.g., antigens and allergens) can be prepared recombinantly, chemically synthesized, isolated from a biological material or source, and optionally modified, or any combination thereof. A biological material or source would include any organism of any proteins or peptide (e.g., antigen or allergen) set forth herein (e.g., as listed in Tables 1 to 8), or any part or product of any organism set forth herein (e.g., as listed in Tables 1 to 8). A biological material or source may further refer to a preparation in which the morphological integrity or physical state has been altered, modified or disrupted, for example, by dissection, dissociation, solubilization, fractionation, homogenization, biochemical or chemical extraction, pulverization, lyophilization, sonication or any other means of manipulating or processing a biological source or material. Subsequences, variants, homologues and derivatives can be prepared, for example, by substituting, deleting or adding one or more amino acid residues in the amino acid sequence of a protein, peptide, subsequence, portion or homologue thereof, and screening for biological activity, for example eliciting an immune response. A skilled person will understand how to make such derivatives or variants, using standard molecular biology techniques and methods, described for example in Sambrook et al. (2001) Molecular Cloning: a Laboratory Manual, 3^(rd) ed., Cold Spring Harbour Laboratory Press).

The invention also provides protein or peptide (e.g., proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of any of Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)), immobilized on or attached to a substrate. The protein or peptide (e.g., proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of any of Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) can optionally have a unique or distinct position or address on the substrate.

Substrates to which protein or peptide (e.g., proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of any of Table 5, Table 6, Table 7 or Table 8), can be immobilized or attached include essentially any physical entity such as a two dimensional surface that is permeable, semi-permeable or impermeable, either rigid or pliable and capable of either storing, binding to or having attached thereto or impregnated.

Substrates include dry solid medium (e.g., cellulose, polyester, nylon, or mixtures thereof etc.), such as glass, silica, plastic, polyethylene, polystyrene, polypropylene, polyacetate, polycarbonate, polyamide, polyester, polyurethane, or polyvinylchloride. Substrates include structures having sections, compartments, wells, containers, vessels or tubes, separated from each other to avoid or prevent cross-contamination or mixing with each other or with other reagents. Multi-well plates, which typically contain 6, 12, 26, 48, 96, to 1000 wells, are one particular non-limiting example of such a structure.

Substrates also include supports used for two- or three-dimensional arrays of sequences. The sequences are typically attached to the surface of the substrate (e.g., via a covalent bond) at defined positions (locations or addresses). Substrates can include a number of sequences, for example, 1, 2, 3, 4, 5, 5 to 10, 10 to 15, 15 to 20, 20 to 25, 25 to 30, 30 to 35, 35 to 40, 40 to 45, 45 to 50, 50 to 75, 75 to 100, 100 to 150, 150 to 200, 200 to 250, 250 to 300, up to all proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, listed in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). Such substrates, also referred to as “arrays,” can have any protein density; the greater the density the greater the number of sequences that can be screened on a given chip. Substrates that include a two- or three-dimensional array of sequences, and individual protein sequences therein, may be coded in accordance with the invention.

The invention also provides nucleic acids encoding proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of amino acid sequences set forth in Table 5, Table 6, Table 7 or Table 8. Such nucleic acid sequences encode a sequence at least 40% or more (e.g., 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%) identical to an exemplary protein, peptide, subsequence, portion, homologue, variant or derivative thereof, for example, of any amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In an additional embodiment, a nucleic acid encodes a sequence having a modification, such as one or more amino acid additions (insertions), deletions or substitutions of protein, peptide, subsequence, portion, homologue, variant or derivative thereof, for example, of an amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance) 8.

The terms “nucleic acid,” “polynucleotide” and “polynucleoside” and the like refer to at least two or more ribo- or deoxy-ribonucleic acid base pairs (nucleotides/nucleosides) that are linked through a phosphoester bond or equivalent. Nucleic acids include polynucleotides and polynucleosides. Nucleic acids include single, double or triplex, circular or linear, molecules. Exemplary nucleic acids include but are not limited to: RNA, DNA, cDNA, genomic nucleic acid, naturally occurring and non-naturally occurring nucleic acid, e.g., synthetic nucleic acid.

Nucleic acids can be of various lengths. Nucleic acid lengths typically range from about 20 bases to 20 Kilobases (Kb), or any numerical value or range within or encompassing such lengths, 10 bases to 10 Kb, 1 to 5 Kb or less, 1000 to about 500 bases or less in length. Nucleic acids can also be shorter, for example, 100 to about 500 bases, or from about 12 to 24, 24 to 45, 45 to 90, 90 to 250, or about 250 to 500 bases in length, or any numerical value or range or value within or encompassing such lengths. In particular aspects, a nucleic acid sequence has a length from about 10-20, 20-30, 30-50, 50-100, 100-150, 150-200, 200-250, 250-300, 300-400, 400-500, 500-1000, 1000-2000 bases, or any numerical value or range within or encompassing such lengths. Shorter nucleic acids are commonly referred to as “oligonucleotides” or “probes” of single- or double-stranded DNA. However, there is no upper limit to the length of such oligonucleotides.

Nucleic acid sequences further include nucleotide and nucleoside substitutions, additions and deletions, as well as derivatized forms and fusion/chimeric sequences (e.g., encoding recombinant polypeptide). For example, due to the degeneracy of the genetic code, nucleic acids include sequences and subsequences degenerate with respect to nucleic acids that encode proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, (e.g., substitutions, additions, insertions and deletions), for example, of amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance).

Nucleic acids can be produced using various standard cloning and chemical synthesis techniques. Techniques include, but are not limited to nucleic acid amplification, e.g., polymerase chain reaction (PCR), with genomic DNA or cDNA targets using primers (e.g., a degenerate primer mixture) capable of annealing to the encoding sequence. Nucleic acids can also be produced by chemical synthesis (e.g., solid phase phosphoramidite synthesis) or transcription from a gene. The sequences produced can then be translated in vitro, or cloned into a plasmid and propagated and then expressed in a cell (e.g., a host cell such as eukaryote or mammalian cell, yeast or bacteria, in an animal or in a plant).

Nucleic acid may be inserted into a nucleic acid construct in which expression of the nucleic acid is influenced or regulated by an “expression control element.” An “expression control element” refers to a nucleic acid sequence element that regulates or influences expression of a nucleic acid sequence to which it is operatively linked. Expression control elements include, as appropriate, promoters, enhancers, transcription terminators, gene silencers, a start codon (e.g., ATG) in front of a protein-encoding gene, etc.

An expression control element operatively linked to a nucleic acid sequence controls transcription and, as appropriate, translation of the nucleic acid sequence. Expression control elements include elements that activate transcription constitutively, that are inducible (i.e., require an external signal for activation), or derepressible (i.e., require a signal to turn transcription off; when the signal is no longer present, transcription is activated or “derepressed”), or specific for cell-types or tissues (i.e., tissue-specific control elements).

Nucleic acid can also be inserted into a plasmid for propagation into a host cell and for subsequent genetic manipulation. A plasmid is a nucleic acid that can be propagated in a host cell, plasmids may optionally contain expression control elements in order to drive expression of the nucleic acid encoding proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof in the host cell. A vector is used herein synonymously with a plasmid and may also include an expression control element for expression in a host cell (e.g., expression vector). Plasmids and vectors generally contain at least an origin of replication for propagation in a cell and a promoter. Plasmids and vectors are therefore useful for genetic manipulation and expression of proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of amino acid sequences set forth in Table 5, Table 6, Table 7 or Table 8. Accordingly, vectors that include nucleic acids encoding or complementary to proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), are provided.

In accordance with the invention, there are provided particles (e.g., viral particles) and transformed host cells that express and/or are transformed with a nucleic acid that encodes and/or express proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). Particles and transformed host cells include but are not limited to virions, and prokaryotic and eukaryotic cells such as bacteria, fungi (yeast), plant, insect, and animal (e.g., mammalian, including primate and human, CHO cells and hybridomas) cells. For example, bacteria transformed with recombinant bacteriophage nucleic acid, plasmid nucleic acid or cosmid nucleic acid expression vectors; yeast transformed with recombinant yeast expression vectors; plant cell systems infected with recombinant virus expression vectors (e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or transformed with recombinant plasmid expression vectors (e.g., Ti plasmid); insect cell systems infected with recombinant virus expression vectors (e.g., baculovirus); and animal cell systems infected with recombinant virus expression vectors (e.g., retroviruses, adenovirus, vaccinia virus), or transformed animal cell systems engineered for stable expression. The cells may be a primary cell isolate, cell culture (e.g., passaged, established or immortalized cell line), or part of a plurality of cells, or a tissue or organ ex vivo or in a subject (in vivo).

The term “transformed” or “transfected” when used in reference to a cell (e.g., a host cell) or organism, means a genetic change in a cell following incorporation of an exogenous molecule, for example, a protein or nucleic acid (e.g., a transgene) into the cell. Thus, a “transfected” or “transformed” cell is a cell into which, or a progeny thereof in which an exogenous molecule has been introduced by the hand of man, for example, by recombinant DNA techniques.

The nucleic acid or protein can be stably or transiently transfected or transformed (expressed) in the host cell and progeny thereof. The cell(s) can be propagated and the introduced protein expressed, or nucleic acid transcribed. A progeny of a transfected or transformed cell may not be identical to the parent cell, since there may be mutations that occur during replication.

Expression of proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof and nucleic acid in particles or introduction into target cells (e.g., host cells) can also be carried out by methods known in the art. Non-limiting examples include osmotic shock (e.g., calcium phosphate), electroporation, microinjection, cell fusion, etc. Introduction of nucleic acid and polypeptide in vitro, ex vivo and in vivo can also be accomplished using other techniques. For example, a polymeric substance, such as polyesters, polyamine acids, hydrogel, polyvinyl pyrrolidone, ethylene-vinylacetate, methylcellulose, carboxymethylcellulose, protamine sulfate, or lactide/glycolide copolymers, polylactide/glycolide copolymers, or ethylenevinylacetate copolymers. A nucleic acid can be entrapped in microcapsules prepared by coacervation techniques or by interfacial polymerization, for example, by the use of hydroxymethylcellulose or gelatin-microcapsules, or poly(methylmethacrolate) microcapsules, respectively, or in a colloid system. Colloidal dispersion systems include macromolecule complexes, nano-capsules, microspheres, beads, and lipid-based systems, including oil-in-water emulsions, micelles, mixed micelles, and liposomes.

Liposomes for introducing various compositions into cells are known in the art and include, for example, phosphatidylcholine, phosphatidylserine, lipofectin and DOTAP (e.g., U.S. Pat. Nos. 4,844,904, 5,000,959, 4,863,740, and 4,975,282; and GIBCO-BRL, Gaithersburg, Md.). piperazine based amphilic cationic lipids useful for gene therapy also are known (see, e.g., U.S. Pat. No. 5,861,397). Cationic lipid systems also are known (see, e.g., U.S. Pat. No. 5,459,127). Polymeric substances, microcapsules and colloidal dispersion systems such as liposomes are collectively referred to herein as “vesicles.” Accordingly, viral and non-viral vector means delivery into cells are included.

Proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, for example, of amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), are provided, can be employed in various methods and uses. Such methods and uses include, for example, administration in vitro and in vivo of one or more proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, such as the amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance), or subsequences, portions, homologues, variants or derivatives thereof. The methods and uses provided include methods and uses of modulating an immune response, including, among others, methods and uses of protecting and treating subjects against a disorder, disease; and methods and uses of providing specific immunotherapy; and methods and uses of diagnosis.

In particular embodiments, methods and uses include administration or delivery of a protein, peptide, subsequence, portion, homologue, variants or derivative thereof described herein (e.g., of any amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) to modulate an immune response in a subject, including, for example, modulating an immune response to an allergen or antigen.

As used herein, the term “modulate,” means an alteration or effect on the term modified. For example, the term modulate can be used in various contexts to refer to an alteration or effect of an activity, a function, or expression of a polypeptide, gene or signaling pathway, or a physiological condition or response of an organism. In certain embodiments, modulating involves decreasing, reducing, inhibiting, suppressing or disrupting an immune response of a subject to an antigen or allergen. In other embodiments, modulating involves eliciting, stimulating, inducing, promoting, increasing or enhancing an immune response of a subject to an antigen or allergen. Thus, where the term “modulate” is used to modify the term “immune response against an allergen in a subject” this means that the immune response in the subject to the allergen is altered or affected (e.g., decreased, reduced, inhibited, suppressed, limited, controlled, prevented, elicited, promoted, stimulated, increased, induced, enhanced, etc.).

Methods and uses of modulating an immune response against an antigen or allergen as described herein may be used to provide a subject with protection against an allergic response or reaction to the allergen, or allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the allergen. Accordingly, in other embodiments, methods and uses include administering a protein, peptide, subsequence, portion, homologue, variant or derivative thereof described herein (e.g., of any amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) to protect or treat a subject against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. In still other embodiments, methods and uses include administering or delivering a protein, peptide, subsequence, portion, homologue, variant or derivative thereof described herein (e.g., of any amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) to elicit, stimulate, induce, promote, increase or enhance immunological tolerance of a subject to an antigen or allergen.

In various embodiments, there are provided methods and uses of providing a subject with protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. In various aspects, a method or use includes administering to the subject an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof described herein (e.g., of any amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) sufficient to provide the subject with protection against the allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the allergen.

Methods and uses of the invention include providing a subject with protection against an antigen or allergen, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the exposure to the antigen or allergen, such as vaccinating the subject to protect against an allergic response to the allergen, for example with any amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance). In certain embodiments, methods and uses include protecting the subject against an allergic response or reaction by inducing tolerance of the subject (desensitizing) to the allergen.

As used herein, the terms “protection”, “protect” and grammatical variations thereof, when used in reference to an allergic response or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the exposure to allergen, means preventing an allergic response, reaction, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the exposure to the allergen, or reducing or decreasing susceptibility to an allergic response, reaction, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the exposure to the allergen.

An allergic response includes but is not limited to an allergic reaction, hypersensitivity, an inflammatory response or inflammation. In certain embodiments allergic response may involve one or more of cell infiltration, production of antibodies, production of cytokines, lymphokines, chemokines, interferons and interleukins, cell growth and maturation factors (e.g., differentiation factors), cell proliferation, cell differentiation, cell accumulation or migration (chemotaxis) and cell, tissue or organ damage or remodeling. In particular aspects, an allergic response may include Allergic rhinitis; Onchocercal dermatitis; Atopic dermatitis; allergic conjunctivitis; Drug reactions; Nodules, eosinophilia, rheumatism, dermatitis, rashes, hives, and swelling (NERDS); esophageal and a gastrointestinal allergy.

Allergic responses can occur systemically, or locally in any region, organ, tissue, or cell. In particular aspects, an allergic response occurs in the skin, the upper respiratory tract, the lower respiratory tract, pancreas, thymus, kidney, liver, spleen, muscle, nervous system, skeletal joints, eye, mucosal tissue, gut or bowel.

Methods and uses herein include treating a subject for an allergic response, allergic disorder or allergic disease, as well as one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. Such methods and uses include administering to a subject an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof described herein (e.g., any amino acid sequence set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) sufficient to treat the subject for the allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the allergen.

As will be understood by a person skilled in the art, treating an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen may include decreasing, reducing, inhibiting, suppressing, limiting, controlling or clearing an allergic response, an allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the allergen. Thus in certain embodiments, a method or use of treating a subject for a an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen comprises elimination of the allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the allergen from a subject. In other embodiments, a method or use of treating a subject for an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen includes reducing occurrence, frequency, severity, progression, or duration of the allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the allergen in the subject. In yet another embodiment, a method or use of treating a subject for an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, includes stabilizing the allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with the allergen in a subject by preventing an increase in the occurrence, frequency, severity, progression, or duration of the allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with contact of the subject with an allergen.

Methods and use of the invention include treating or administering a subject previously exposed to an antigen or allergen. Thus, in certain embodiments, methods and uses are for treating or protecting a subject from an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with secondary or subsequent exposure to an antigen or allergen.

Physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen treatable in accordance with the invention methods and uses include but are not limited to asthma, allergic asthma, bronchiolitis and pleuritis, Allergic rhinitis; Onchocercal dermatitis; Atopic dermatitis; allergic conjunctivitis; Drug reactions; Nodules, eosinophilia, rheumatism, dermatitis, rashes, hives, and swelling (NERDS); esophageal and a gastrointestinal allergy, Airway Obstruction, Apnea, Asbestosis, Atelectasis, Berylliosis, Bronchiectasis, Bronchiolitis, Bronchiolitis Obliterans Organizing Pneumonia, Bronchitis, Bronchopulmonary Dysplasia, Empyema, Pleural Empyema, Pleural Epiglottitis, Hemoptysis, Hypertension, Kartagener Syndrome, Meconium Aspiration, Pleural Effusion, Pleurisy, Pneumonia, Pneumothorax, Respiratory Distress Syndrome, Respiratory Hypersensitivity, Rhinoscleroma, Scimitar Syndrome, Severe Acute Respiratory Syndrome, Silicosis, Tracheal Stenosis, eosinophilic pleural effusions, Histiocytosis; chronic eosinophilic pneumonia; hypersensitivity pneumonitis; Allergic bronchopulmonary aspergillosis; Sarcoidosis; Idiopathic pulmonary fibrosis; pulmonary edema; pulmonary embolism; pulmonary emphysema; Pulmonary Hyperventilation; Pulmonary Alveolar Proteinosis; Chronic Obstructive Pulmonary Disease (COPD); Interstitial Lung Disease; and Topical eosinophilia.

Methods and uses of the invention further include inducing immunological tolerance of a subject to an antigen or allergen. In one embodiment, a method or use includes administering to the subject an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof described herein (e.g., any amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411, respectively, in order of appearance), Table 6, Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs:1,907-2,008, respectively, in order of appearance)) sufficient to induce tolerance in the subject to the antigen or allergen.

In additional embodiments, a method or use of inducing immunological tolerance in a subject to an allergen includes a reduction in occurrence, frequency, severity, progression, or duration of physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated an allergic response to the allergen in the subject. Thus, in certain embodiments, inducing immunological tolerance can protect a subject against or treat a subject for an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen or allergen.

Methods and uses of inducing immunological tolerance described herein may include eliciting, stimulating, inducing, promoting, increasing or enhancing an immune response. In certain embodiments, inducing immunological tolerance may include eliciting, stimulating, inducing, promoting, increasing or enhancing an immune response that decreases, reduces, inhibits, suppresses, limits, controls or clears an allergic response. For example, in certain embodiments inducing immunological tolerance may include eliciting, stimulating, inducing, promoting, increasing or enhancing proliferation or activity of regulatory T cells. In other embodiments, inducing immunological tolerance may include eliciting, stimulating, inducing, promoting, increasing or enhancing an immune response that promotes an allergic response. As will be understood by a person of skill in the art, a method or use that elicits, stimulates, induces, promotes, increases or enhances an immune response that promotes an allergic response may still induce immunological tolerance by also eliciting, stimulating, inducing, promoting, increasing or enhancing an immune response that decreases, reduces, inhibits, suppresses, limits, controls or clears an allergic response. In particular embodiments, inducing immunological tolerance includes eliciting, stimulating, inducing, promoting, increasing or enhancing an immune responses that decreases, reduces, inhibits, suppresses, limits, controls or clears an allergic response that is stronger than the immune response that promotes an allergic response. In other embodiments, inducing immunological tolerance includes eliciting, stimulating, inducing, promoting, increasing or enhancing more immune responses that decrease, reduce, inhibit, suppress, limit, controls or clear an allergic response than immune responses that promote an allergic response.

Methods and uses of the invention include treating a subject via specific immunotherapy. In one embodiment, a method or use includes administering to the subject an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof described herein (e.g., any amino acid sequences set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008). In one aspect, an antigen (allergen) administered to a subject during specific immunotherapy to treat the subject is the same antigen (allergen) to which the subject has been sensitized or is hypersensitive (e.g., allergic). In another non-limiting aspect, an antigen (allergen) administered to a subject to treat the subject is a different antigen (allergen) to which the subject has been sensitized or is hypersensitive (e.g., allergic). Thus in different embodiments, the antigen administered and antigen (e.g., allergen) against which immunological tolerance is sought may be the same protein (antigen, allergen), may be proteins (antigens, allergens) of the same organism or may be proteins (antigens, allergens) of different organisms.

In accordance with the invention, methods and uses include therapeutic (following antigen/allergen exposure) and prophylactic (prior to antigen/allergen exposure) uses and methods. For example, therapeutic and prophylactic methods and uses of treating a subject for an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, include but are not limited to treatment of a subject having or at risk of having an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen; treating a subject with an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen; and methods and uses of protecting a subject from an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen (e.g., provide the subject with protection against an allergic reaction to an allergen), to decrease or reduce the probability of an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, in a subject and to decrease or reduce susceptibility of a subject to an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, to inhibit or prevent an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, in a subject. Accordingly, methods and uses can treat an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, or provide a subject with protection from an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen (e.g., prophylactic protection).

As described herein, proteins, peptides, subsequences, portions, homologues, variants and derivatives thereof include T cell epitopes. In Accordingly, methods and uses of the invention include administering an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof (e.g., a T cell epitope) to a subject sufficient to provide the subject with protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. In another embodiment, a method includes administering an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof (e.g., a T cell epitope) to a subject sufficient to treat, vaccinate or immunize the subject against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen.

In accordance with the invention, methods and uses of modulating anti-allergen activity of T cells, including but not limited to CD8⁺ T cells, CD4⁺ T cells, Th1 cells or Th2 cells, in a subject are provided. In one embodiment, a method or use includes administering to a subject an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, such as a T cell epitope, sufficient to modulate Th2 cell activity in the subject.

In all methods and uses of the invention, any appropriate protein, peptide, subsequence, portion, homologue, variant or derivative thereof can be used or administered. In particular non-limiting examples, the protein, peptide, subsequence, portion, homologue, variant or derivative thereof comprises, consists of or consists essentially of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008), or a subsequence, portion, homologue, variant or derivative thereof.

In certain embodiments, two or more proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, may be administered to a subject. In particular embodiments, a protein, peptide, subsequence, portion, homologue, variant or derivative thereof consists of or consists essentially of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008), or subsequence, portion, homologue, variant or derivative thereof, and is administered with one or more other proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof. Two or more proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof may be administered as a combination composition, or administered separately, such as concurrently or in series or sequentially. Different proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, may be administered to a subject in the same amount, volume or concentration, or different amounts, volumes or concentrations. Thus, in certain embodiments, the subject may be administered the same amount of two or more different proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof; and in other embodiments, the subject may be administered one protein, peptide, subsequence, portion, homologue, variant or derivative thereof in an amount, volume or concentration greater than one or more other protein, peptide, subsequence, portion, homologue, variant or derivative thereof administered to the subject.

Methods and uses of the invention include a favorable response or an improvement in one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen. In particular embodiments, a favorable response or improvement includes but is not limited to reduce, decrease, suppress, limit, control or inhibit an allergic response including reducing, decreasing, suppressing, limiting, controlling or inhibiting immune cell proliferation, function or activity, or eliciting, stimulating, inducing, promoting, increasing or enhancing immune cell proliferation or activity (e.g. regulatory T cells); or reduce, decrease, suppress, limit, control or inhibit the amount of allergen. In additional particular embodiments, an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof is sufficient to elicit, stimulate, induce, promote, increase or enhance or augment immunological tolerance to an allergen; or decrease, reduce, inhibit, suppress, prevent, control, or limit an allergic reaction or response. In further particular embodiments, an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof is sufficient to protect a subject from an allergic response or reduce, decrease, limit, control or inhibit susceptibility to an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen.

Methods and uses of the invention therefore include any therapeutic or beneficial effect. In various methods embodiments, an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen is reduced, decreased, inhibited, limited, delayed or prevented. Physiological conditions, disorders, illnesses and diseases associated with an antigen/allergen include but are not limited to asthma, allergic asthma, bronchiolitis and pleuritis, Allergic rhinitis; Onchocercal dermatitis; Atopic dermatitis; allergic conjunctivitis; Drug reactions; Nodules, eosinophilia, rheumatism, dermatitis, rashes, hives, and swelling (NERDS); esophageal and a gastrointestinal allergy, Airway Obstruction, Apnea, Asbestosis, Atelectasis, Berylliosis, Bronchiectasis, Bronchiolitis, Bronchiolitis Obliterans Organizing Pneumonia, Bronchitis, Bronchopulmonary Dysplasia, Empyema, Pleural Empyema, Pleural Epiglottitis, Hemoptysis, Hypertension, Kartagener Syndrome, Meconium Aspiration, Pleural Effusion, Pleurisy, Pneumonia, Pneumothorax, Respiratory Distress Syndrome, Respiratory Hypersensitivity, Rhinoscleroma, Scimitar Syndrome, Severe Acute Respiratory Syndrome, Silicosis, Tracheal Stenosis, eosinophilic pleural effusions, Histiocytosis; chronic eosinophilic pneumonia; hypersensitivity pneumonitis; Allergic bronchopulmonary aspergillosis; Sarcoidosis; Idiopathic pulmonary fibrosis; pulmonary edema; pulmonary embolism; pulmonary emphysema; Pulmonary Hyperventilation; Pulmonary Alveolar Proteinosis; Chronic Obstructive Pulmonary Disease (COPD); Interstitial Lung Disease; and Topical eosinophilia. Symptoms and complications associated with an allergen include but are not limited to cell infiltration, production of antibodies, production of cytokines, lymphokines, chemokines, interferons and interleukins, cell growth and maturation factors (e.g., differentiation factors), cell proliferation, cell differentiation, cell accumulation or migration and cell, tissue or organ damage or remodelling, allergic rhinitis; Onchocercal dermatitis; Atopic dermatitis; allergic conjunctivitis; Drug reactions; Nodules, eosinophilia, rheumatism, dermatitis, rashes, hives, and swelling (NERDS); esophageal and a gastrointestinal allergy. Additional symptoms of antigen/allergen exposure are known to one of skill in the art and treatment thereof in accordance with the invention is provided.

Methods and uses of the invention moreover include reducing, decreasing, inhibiting, delaying or preventing onset, progression, frequency, duration, severity, probability or susceptibility of one or more adverse symptoms, disorders, illnesses, diseases or complications caused by or associated with an antigen/allergen. In further various particular embodiments, methods and uses include improving, accelerating, facilitating, enhancing, augmenting, or hastening recovery of a subject from an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen. In yet additional various embodiments, methods and uses include stabilizing an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen.

A therapeutic or beneficial effect is therefore any objective or subjective measurable or detectable improvement or benefit provided to a particular subject. A therapeutic or beneficial effect can but need not be complete ablation of all or any allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. Thus, a satisfactory clinical endpoint is achieved when there is an incremental improvement or a partial reduction in an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, or an inhibition, decrease, reduction, suppression, prevention, limit or control of worsening or progression of an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, over a short or long duration (hours, days, weeks, months, etc.).

A therapeutic or beneficial effect also includes reducing or eliminating the need, dosage frequency or amount of a second therapeutic protocol or active such as another drug or other agent (e.g., anti-inflammatory) used for treating a subject having or at risk of having an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. For example, reducing an amount of an adjunct therapy, such as a reduction or decrease of a treatment for an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, or a specific immunotherapy, vaccination or immunization protocol is considered a beneficial effect. In addition, reducing or decreasing an amount of protein, peptide, subsequence, portion, homologue, variant or derivative thereof, used for specific immunotherapy, vaccination or immunization of a subject to provide protection to the subject is considered a beneficial effect.

As disclosed herein, invention proteins, peptides, subsequences, etc., can be used in methods of providing specific immunotherapy to a subject, such as a subject with or at risk of an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. In one embodiment, a method or use includes administering to a subject an amount of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof sufficient to elicit, stimulate, induce, promote, increase, enhance or augment immunological tolerance in the subject to an antigen/allergen. In another embodiment, a method includes administering to a subject an amount of a nucleic acid encoding all or a portion (e.g., a T cell epitope) of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof sufficient to elicit, stimulate, induce, promote, increase, enhance or augment immunological tolerance of the subject to an allergen.

When an antigen(s) or allergen(s) is administered to induce tolerance, an amount or dose of the antigen or allergen to be administered, and the period of time required to achieve a desired outcome or result (e.g., to desensitize or develop tolerance to the antigen or allergen) can be determined by one skilled in the art. The antigen or allergen may be administered to the patient through any route known in the art, including, but not limited to oral, inhalation, sublingual, epicutaneous, intranasal, and/or parenteral routes (intravenous, intramuscular, subcutaneously, and intraperitoneal).

Methods and uses of the invention include administration of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof to a subject prior to contact by or exposure to an allergen; administration prior to, substantially contemporaneously with or after a subject has been contacted by or exposed to an allergen; and administration prior to, substantially contemporaneously with or after an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. A subject contacted by or exposed to an allergen may have contact or exposure over a period of 1-5, 5-10, 10-20, 20-30, 30-50, 50-100 hours, days, months, or years.

Invention compositions (e.g., proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, including T cell epitopes, for example, of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008), methods and uses can be combined with any compound, agent, drug, treatment or other therapeutic regimen or protocol having a desired therapeutic, beneficial, additive, synergistic or complementary activity or effect. Exemplary combination compositions and treatments include multiple proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof such as T cell epitopes as described herein (e.g., of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)), and second actives, such as anti-allergen compounds, agents, drugs, treatments and therapies, including but not limited to anti-histamines, anti-inflammatories, decongestants and corticosteroids as well as agents that assist, promote, stimulate or enhance efficacy. Such anti-allergen drugs, agents, treatments and therapies can be administered or performed prior to, substantially contemporaneously with or following any method or use described herein, for example, a therapeutic use or method of treating a subject for an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, or a method or use of providing specific immunotherapy to a subject.

Accordingly, methods and uses include combinations of proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof and second actives, and administering as a combination with a second active, or administered separately, such as concurrently or in series or sequentially (prior to or following) to administering a second active to a subject. The invention therefore provides combinations of one or more proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, in combination with a second active, including but not limited to any compound, agent, drug, therapeutic regimen, treatment protocol, process, remedy or composition, such as anti-histamine, anti-inflammatory, decongestant and corticosteroid, or immune tolerance stimulating, enhancing or augmenting protocol, or specific immunotherapy protocol set forth herein or known in the art. The compound, agent, drug, therapeutic regimen, treatment protocol, process, remedy or composition can be administered or performed prior to, substantially contemporaneously with or following administration of one or more proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, or a nucleic acid encoding all or a portion (e.g., a T cell epitope) of a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, to a subject. Specific non-limiting examples of combination embodiments therefore include the foregoing or other compound, agent, drug, therapeutic regimen, treatment protocol, process, remedy or composition.

An exemplary combination is a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, and a different protein, peptide, or subsequence, portion, homologue, variant or derivative thereof, of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008). Another exemplary combination is a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, and an immunological tolerance inducing molecule.

In invention methods and uses in which there is a desired outcome or effect, such as a therapeutic or prophylactic method or use that provides a benefit from treatment, protection, inducing immunological tolerance, vaccination or specific immunotherapy, a protein, peptide, subsequence, portion, homologue, variant or derivative thereof can be administered in a sufficient or effective amount. As used herein, a “sufficient amount” or “effective amount” or an “amount sufficient” or an “amount effective” refers to an amount that provides, in single (e.g., primary) or multiple (e.g., booster) doses, alone or in combination with one or more other compounds, treatments, therapeutic regimens or agents (e.g., a drug), a long term or a short term detectable or measurable improvement in a given subject or any objective or subjective benefit to a given subject of any degree or for any time period or duration (e.g., for minutes, hours, days, months, years, or cured).

An amount sufficient or an amount effective can but need not be provided in a single administration and can but need not be achieved by a particular protein, peptide, subsequence, portion, homologue, variant or derivative thereof, alone, optionally in a combination composition or method or use that includes a second active. In addition, an amount sufficient or an amount effective need not be sufficient or effective if given in single or multiple doses without a second or additional administration or dosage, since additional doses, amounts or duration above and beyond such doses, or additional antigens, compounds, drugs, agents, treatment or therapeutic regimens may be included in order to provide a given subject with a detectable or measurable improvement or benefit to the subject. For example, to increase, enhance, improve or optimize specific immunotherapy, after an initial or primary administration of one or more proteins, peptides, subsequences, portions, homologues, variants or derivative thereof, the subject can be administered one or more additional “boosters” of one or more proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof. Such subsequent “booster” administrations can be of the same or a different type, formulation, dose, concentration, route, etc.

An amount sufficient or an amount effective need not be therapeutically or prophylactically effective in each and every subject treated, nor a majority of subjects treated in a given group or population. An amount sufficient or an amount effective means sufficiency or effectiveness in a particular subject, not a group of subjects or the general population. As is typical for such methods, different subjects will exhibit varied responses to a method of the invention, such as immunization, vaccination, specific immunotherapy and therapeutic treatments.

The term “subject” includes but is not limited to a subject at risk of allergen contact or exposure as well as a subject that has been contacted by or exposed to an allergen. A subject also includes those having or at risk of having or developing an immune response to an antigen or an allergen. Such subjects include mammalian animals (mammals), such as a non-human primate (apes, gibbons, gorillas, chimpanzees, orangutans, macaques), a domestic animal (dogs and cats), a farm animal (poultry such as chickens and ducks, horses, cows, goats, sheep, pigs), experimental animal (mouse, rat, rabbit, guinea pig) and humans. Subjects include animal disease models, for example, mouse and other animal models of allergic response known in the art.

Accordingly, subjects appropriate for treatment include those having or at risk of exposure to an antigen or allergen, also referred to as subjects in need of treatment. Subjects in need of treatment therefore include subjects that have been exposed to or contacted with an antigen or allergen, or that have an ongoing contact or exposure or have developed one or more adverse symptoms caused by or associated with an antigen or allergen, regardless of the type, timing or degree of onset, progression, severity, frequency, duration of the symptoms.

Target subjects and subjects in need of treatment also include those at risk of allergen exposure or contact or at risk of having exposure or contact to an allergen. Accordingly, subjects include those at increased or elevated (high) risk of an allergic reaction; has, or has previously had or is at risk of developing hypersensitivity to an allergen; and those that have or have previously had or is at risk of developing asthma.

The invention compositions, methods and uses are therefore applicable to treating a subject who is at risk of allergen exposure or contact but has not yet been exposed to or contacted with the allergen. Prophylactic uses and methods are therefore included. Target subjects for prophylaxis may be at increased risk (probability or susceptibility) of allergen exposure or contact as set forth herein. Such subjects are considered in need of treatment due to being at risk.

Subjects for prophylaxis need not be at increased risk but may be from the general population in which it is desired to protect a subject against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen or to provide specific immunotherapy, for example. Such a subject that is desired to be protected against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen or to be provided specific immunotherapy can be administered a protein, peptide, subsequence, portion, homologue, variant or derivative thereof. In another non-limiting example, a subject that is not specifically at risk of exposure to or contact by an allergen, but nevertheless desires protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, can be administered a protein, peptide, subsequence, portion, homologue, variant or derivative thereof. Such subjects are also considered in need of treatment.

“Prophylaxis” and grammatical variations thereof mean a method or use in which contact, administration or in vivo delivery to a subject is prior to contact with or exposure to an allergen. In certain situations it may not be known that a subject has been contacted with or exposed to an allergen, but administration or in vivo delivery to a subject can be performed prior to manifestation of an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. For example, a subject can be provided protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen or provided specific immunotherapy with a protein, peptide, subsequence, portion, homologue, variant or derivative thereof. In such case, a method or use can eliminate, prevent, inhibit, suppress, limit, decrease or reduce the probability of or susceptibility towards an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen.

“Prophylaxis” can also refer to a method or use in which contact, administration or in vivo delivery to a subject is prior to a secondary or subsequent exposure to an antigen/allergen. In such a situation, a subject may have had a prior contact or exposure to an allergen. In such subjects, an acute allergic reaction may but need not be resolved. Such a subject typically may have developed anti-allergen antibodies due to the prior exposure. Immunization or vaccination, by administration or in vivo delivery to such a subject, can be performed prior to a secondary or subsequent allergen exposure. Such a method or use can eliminate, prevent, inhibit, suppress, limit, decrease or reduce the probability of or susceptibility towards a secondary or subsequent allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. In certain embodiments, such a method or use includes providing specific immunotherapy to the subject to eliminate, prevent, inhibit, suppress, limit, decrease or reduce the probability of or susceptibility towards a secondary or subsequent allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen.

Treatment of an allergic reaction or response can be at any time during the reaction or response. A protein, peptide, subsequence, portion, homologue, variant or derivative thereof, can be administered as a combination (e.g., with a second active), or separately concurrently or in sequence (sequentially) in accordance with the methods and uses described herein as a single or multiple dose e.g., one or more times hourly, daily, weekly, monthly or annually or between about 1 to 10 weeks, or for as long as appropriate, for example, to achieve a reduction in the onset, progression, severity, frequency, duration of one or more symptoms or complications associated with or caused by an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen.

Accordingly, methods and uses of the invention can be practiced one or more times (e.g., 1-10, 1-5 or 1-3 times) an hour, day, week, month, or year. The skilled artisan will know when it is appropriate to delay or discontinue administration. A non-limiting dosage schedule is 1-7 times per week, for 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20 or more weeks.

Doses can be based upon current existing protocols, empirically determined, using animal disease models or optionally in human clinical trials. Initial study doses can be based upon animal studies, e.g. a mouse, and the amount of protein, peptide, subsequence, portion, homologue, variant or derivative thereof, administered that is determined to be effective. Exemplary non-limiting amounts (doses) are in a range of about 0.1 mg/kg to about 100 mg/kg, and any numerical value or range or value within such ranges. Greater or lesser amounts (doses) can be administered, for example, 0.01-500 mg/kg, and any numerical value or range or value within such ranges. The dose can be adjusted according to the mass of a subject, and will generally be in a range from about 1-10 ug/kg, 10-25 ug/kg, 25-50 ug/kg, 50-100 ug/kg, 100-500 ug/kg, 500-1,000 ug/kg, 1-5 mg/kg, 5-10 mg/kg, 10-20 mg/kg, 20-50 mg/kg, 50-100 mg/kg, 100-250 mg/kg, 250-500 mg/kg, or more, two, three, four, or more times per hour, day, week, month or annually. A typical range will be from about 0.3 mg/kg to about 50 mg/kg, 0-25 mg/kg, or 1.0-10 mg/kg, or any numerical value or range or value within such ranges.

Doses can vary and depend upon whether the treatment is prophylactic or therapeutic, whether a subject has been previously exposed to the antigen/allergen, the onset, progression, severity, frequency, duration, probability of or susceptibility of the symptom, condition, pathology or complication, or vaccination or specific immunotherapy to which treatment is directed, the clinical endpoint desired, previous or simultaneous treatments, the general health, age, gender, race or immunological competency of the subject and other factors that will be appreciated by the skilled artisan. The skilled artisan will appreciate the factors that may influence the dosage and timing required to provide an amount sufficient for providing a therapeutic or prophylactic benefit.

Typically, for treatment, a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, will be administered as soon as practical, typically within 1-2, 2-4, 4-12, 12-24 or 24-72 hours after a subject is exposed to or contacted with an allergen, or within 1-2, 2-4, 4-12, 12-24 or 24-48 hours after onset or development of one or more of an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an antigen/allergen.

For prophylactic treatment in connection with vaccination or specific immunotherapy, proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, can be administered for a duration of 0-4 weeks, e.g., 2-3 weeks, prior to exposure to or contact by an allergen or at least within 1-2, 2-4, 4-12, 12-24, 24-48 or 48-72 hours prior to exposure to or contact by an allergen. For an acute allergic reaction, proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof may be administered at any appropriate time.

The dose amount, number, frequency or duration may be proportionally increased or reduced, as indicated by the status of the subject. For example, whether the subject has an allergic response, whether the subject has been exposed to or contacted by an allergen or is merely at risk of allergen contact or exposure, whether the subject is a candidate for or will be vaccinated or provided specific immunotherapy. The dose amount, number, frequency or duration may be proportionally increased or reduced, as indicated by any adverse side effects, complications or other risk factors of the treatment or therapy.

In methods and uses of the invention, the route, dose, number and frequency of administrations, treatments, vaccinations and specific immunotherapy, and timing/intervals between treatment, vaccination and specific immunotherapy, and allergen exposure can be modified. Although rapid induction of immune responses or immunological tolerance is desired for developing protective emergency vaccines against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, in certain embodiments, a desirable treatment will elicit robust, long-lasting protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. Thus, in certain embodiments, invention compositions, methods and uses provide long-lasting protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen. Specific immunotherapy strategies can provide long-lived protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen depending on the level of induced immunological tolerance or a T cell response or activity.

Compositions, methods and uses include pharmaceutical compositions and formulations. In certain embodiments, a pharmaceutical composition includes one or more proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof described herein (e.g., an amino acid sequence of a protein or peptide set forth in Table 5, Table 6, Table 7 or Table 8). In particular, aspects, such compositions and formulations may be a vaccine, including but not limited to a vaccine to protect against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen.

As used herein the term “pharmaceutically acceptable” and “physiologically acceptable” mean a biologically acceptable formulation, gaseous, liquid or solid, or mixture thereof, which is suitable for one or more routes of administration, in vivo delivery or contact. Such formulations include solvents (aqueous or non-aqueous), solutions (aqueous or non-aqueous), emulsions (e.g., oil-in-water or water-in-oil), suspensions, syrups, elixirs, dispersion and suspension media, coatings, isotonic and absorption promoting or delaying agents, compatible with pharmaceutical administration or in vivo contact or delivery. Aqueous and non-aqueous solvents, solutions and suspensions may include suspending agents and thickening agents. Such pharmaceutically acceptable carriers include tablets (coated or uncoated), capsules (hard or soft), microbeads, powder, granules and crystals. Supplementary active compounds (e.g., preservatives, antibacterial, antiviral and antifungal agents) can also be incorporated into the compositions.

To increase an immune response, immunological tolerance or protection against an allergic response, allergic disorder or allergic disease, or one or more physiological conditions, disorders, illnesses, diseases, symptoms or complications caused by or associated with an allergen, proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof, can be coupled to another protein such as ovalbumin or keyhole limpet hemocyanin (KLH), thyroglobulin or a toxin such as tetanus or cholera toxin. Proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof can also be mixed with adjuvants.

Adjuvants include, for example: oil (mineral or organic) emulsion adjuvants such as Freund's complete (CFA) and incomplete adjuvant (IFA) (WO 95/17210; WO 98/56414; WO 99/12565; WO 99/11241; and U.S. Pat. No. 5,422,109); metal and metallic salts, such as aluminum and aluminum salts, such as aluminum phosphate or aluminum hydroxide, alum (hydrated potassium aluminum sulfate); bacterially derived compounds, such as Monophosphoryl lipid A and derivatives thereof (e.g., 3 De-O-acylated monophosphoryl lipid A, aka 3D-MPL or d3-MPL, to indicate that position 3 of the reducing end glucosamine is de-O-acylated, 3D-MPL consisting of the tri and tetra acyl congeners), and enterobacterial lipopolysaccharides (LPS); plant derived saponins and derivatives thereof, for example Quil A (isolated from the Quilaj a Saponaria Molina tree, see, e.g., “Saponin adjuvants”, Archiv. fur die gesamte Virusforschung, Vol. 44, Springer Verlag, Berlin, p 243-254; U.S. Pat. No. 5,057,540), and fragments of Quil A which retain adjuvant activity without associated toxicity, for example QS7 and QS21 (also known as QA7 and QA21), as described in WO96/33739, for example; surfactants such as, soya lecithin and oleic acid; sorbitan esters such as sorbitan trioleate; and polyvinylpyrrolidone; oligonucleotides such as CpG (WO 96/02555, and WO 98/16247), polyriboA and polyriboU; block copolymers; and immunostimulatory cytokines such as GM-CSF and IL-1, and Muramyl tripeptide (MTP). Additional examples of adjuvants are described, for example, in “Vaccine Design—the subunit and adjuvant approach” (Edited by Powell, M. F. and Newman, M. J.; 1995, Pharmaceutical Biotechnology (Plenum Press, New York and London, ISBN 0-306-44867-X) entitled “Compendium of vaccine adjuvants and excipients” by Powell, M. F. and Newman M.

Cosolvents may be added to a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, composition or formulation. Non-limiting examples of cosolvents contain hydroxyl groups or other polar groups, for example, alcohols, such as isopropyl alcohol; glycols, such as propylene glycol, polyethyleneglycol, polypropylene glycol, glycol ether; glycerol; polyoxyethylene alcohols and polyoxyethylene fatty acid esters. Non-limiting examples of cosolvents contain hydroxyl groups or other polar groups, for example, alcohols, such as isopropyl alcohol; glycols, such as propylene glycol, polyethyleneglycol, polypropylene glycol, glycol ether; glycerol; polyoxyethylene alcohols and polyoxyethylene fatty acid esters.

Supplementary compounds (e.g., preservatives, antioxidants, antimicrobial agents including biocides and biostats such as antibacterial, antiviral and antifungal agents) can also be incorporated into the compositions. Pharmaceutical compositions may therefore include preservatives, anti-oxidants and antimicrobial agents.

Preservatives can be used to inhibit microbial growth or increase stability of ingredients thereby prolonging the shelf life of the pharmaceutical formulation. Suitable preservatives are known in the art and include, for example, EDTA, EGTA, benzalkonium chloride or benzoic acid or benzoates, such as sodium benzoate. Antioxidants include, for example, ascorbic acid, vitamin A, vitamin E, tocopherols, and similar vitamins or provitamins.

An antimicrobial agent or compound directly or indirectly inhibits, reduces, delays, halts, eliminates, arrests, suppresses or prevents contamination by or growth, infectivity, replication, proliferation, reproduction, of a pathogenic or non-pathogenic microbial organism. Classes of antimicrobials include antibacterial, antiviral, antifungal and antiparasitics. Antimicrobials include agents and compounds that kill or destroy (-cidal) or inhibit (-static) contamination by or growth, infectivity, replication, proliferation, reproduction of the microbial organism.

Exemplary antibacterials (antibiotics) include penicillins (e.g., penicillin G, ampicillin, methicillin, oxacillin, and amoxicillin), cephalosporins (e.g., cefadroxil, ceforanid, cefotaxime, and ceftriaxone), tetracyclines (e.g., doxycycline, chlortetracycline, minocycline, and tetracycline), aminoglycosides (e.g., amikacin, gentamycin, kanamycin, neomycin, streptomycin, netilmicin, paromomycin and tobramycin), macrolides (e.g., azithromycin, clarithromycin, and erythromycin), fluoroquinolones (e.g., ciprofloxacin, lomefloxacin, and norfloxacin), and other antibiotics including chloramphenicol, clindamycin, cycloserine, isoniazid, rifampin, vancomycin, aztreonam, clavulanic acid, imipenem, polymyxin, bacitracin, amphotericin and nystatin.

Particular non-limiting classes of anti-virals include reverse transcriptase inhibitors; protease inhibitors; thymidine kinase inhibitors; sugar or glycoprotein synthesis inhibitors; structural protein synthesis inhibitors; nucleoside analogues; and viral maturation inhibitors. Specific non-limiting examples of anti-virals include nevirapine, delavirdine, efavirenz, saquinavir, ritonavir, indinavir, nelfinavir, amprenavir, zidovudine (AZT), stavudine (d4T), larnivudine (3TC), didanosine (DDI), zalcitabine (ddC), abacavir, acyclovir, penciclovir, ribavirin, valacyclovir, ganciclovir, 1,-D-ribofuranosyl-1,2,4-triazole-3 carboxamide, 9->2-hydroxy-ethoxy methylguanine, adamantanamine, 5-iodo-2′-deoxyuridine, trifluorothymidine, interferon and adenine arabinoside.

Pharmaceutical formulations and delivery systems appropriate for the compositions, methods and uses of the invention are known in the art (see, e.g., Remington: The Science and Practice of Pharmacy (2003) 20^(th) ed., Mack Publishing Co., Easton, Pa.; Remington's Pharmaceutical Sciences (1990) 18^(th) ed., Mack Publishing Co., Easton, Pa.; The Merck Index (1996) 12^(th) ed., Merck Publishing Group, Whitehouse, N.J.; Pharmaceutical Principles of Solid Dosage Forms (1993), Technonic Publishing Co., Inc., Lancaster, Pa.; Ansel ad Soklosa, Pharmaceutical Calculations (2001) 11^(th) ed., Lippincott Williams & Wilkins, Baltimore, Md.; and Poznansky et al., Drug Delivery Systems (1980), R. L. Juliano, ed., Oxford, N.Y., pp. 253-315).

Pharmaceutical compositions can be formulated to be compatible with a particular route of administration. Thus, pharmaceutical compositions include carriers, diluents, or excipients suitable for administration by various routes. Exemplary routes of administration for contact or in vivo delivery which a composition can optionally be formulated include inhalation, respiration, intranasal, intubation, intrapulmonary instillation, oral, buccal, intrapulmonary, intradermal, topical, dermal, parenteral, sublingual, subcutaneous, intravascular, intrathecal, intraarticular, intracavity, transdermal, iontophoretic, intraocular, ophthalmic, optical, intravenous (i.v.), intramuscular, intraglandular, intraorgan, or intralymphatic.

Formulations suitable for parenteral administration include aqueous and non-aqueous solutions, suspensions or emulsions of the active compound, which preparations are typically sterile and can be isotonic with the blood of the intended recipient. Non-limiting illustrative examples include water, saline, dextrose, fructose, ethanol, animal, vegetable or synthetic oils.

Methods and uses of the invention may be practiced by any mode of administration or delivery, or by any route, systemic, regional and local administration or delivery. Exemplary administration and delivery routes include intravenous (i.v.), intraperitoneal (i.p.), intraarterial, intramuscular, parenteral, subcutaneous, intra-pleural, topical, dermal, intradermal, transdermal, transmucosal, intra-cranial, intra-spinal, rectal, oral (alimentary), mucosal, inhalation, respiration, intranasal, intubation, intrapulmonary, intrapulmonary instillation, buccal, sublingual, intravascular, intrathecal, intracavity, iontophoretic, intraocular, ophthalmic, optical, intraglandular, intraorgan, or intralymphatic.

For oral administration, a composition can take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (for example, pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (for example, lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (for example, magnesium stearate, talc or silica); disintegrants (for example, potato starch or sodium starch glycolate); or wetting agents (for example, sodium lauryl sulphate). The tablets can be coated by methods known in the art. Liquid preparations for oral administration can take the form of, for example, solutions, syrups or suspensions, or they can be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations can be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (for example, sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (for example, lecithin or acacia); non-aqueous vehicles (for example, almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (for example, methyl or propyl-p-hydroxybenzoates or sorbic acid). The preparations can also contain buffer salts, flavoring, coloring, and sweetening agents as appropriate.

For administration by inhalation, a composition can be delivered in the form of an aerosol spray presentation from pressurized packs or a nebulizer, with the use of a suitable propellant, for example, dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit can be determined by providing a valve to deliver a metered amount. Capsules and cartridges for use in an inhaler or insufflator can be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.

Invention proteins, peptides, subsequences, portions, homologues, variants or derivatives thereof optionally along with any adjunct agent, compound, drug, composition, whether active or inactive, etc., can be packaged in unit dosage form (capsules, tablets, troches, cachets, lozenges) for ease of administration and uniformity of dosage. A “unit dosage form” as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active ingredient optionally in association with a pharmaceutical carrier (excipient, diluent, vehicle or filling agent) which, when administered in one or more doses, is calculated to produce a desired effect (e.g., prophylactic or therapeutic effect). Unit dosage forms also include, for example, ampules and vials, which may include a composition in a freeze-dried or lyophilized state; a sterile liquid carrier, for example, can be added prior to administration or delivery in vivo. Unit dosage forms additionally include, for example, ampules and vials with liquid compositions disposed therein. Individual unit dosage forms can be included in multi-dose kits or containers. Pharmaceutical formulations can be packaged in single or multiple unit dosage form for ease of administration and uniformity of dosage.

The invention also provides methods of diagnosing and detecting an allergic response or allergy in a subject. The methods can be performed in solution, in solid phase, in silica, in vitro, in a cell, and in vivo. In one embodiment, a method includes contacting a cell (e.g., T cell) from the subject with a protein, peptide, subsequence, portion, homologue, variant or derivative thereof, as described herein (e.g., of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)); and determining if the protein or peptide modulates an immune response or activity of the contacted cell (e.g., T cell). A determination that the protein or peptide modulates an immune response or immune activity of the contacted cell indicates that the subject has an allergic response or an allergy, in particular, an allergy to the protein, peptide, subsequence, portion, homologue, variant or derivative thereof (e.g., of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)). In a particular aspect, the immune activity determined is Th2 cell reactivity. In another particular aspect, immune response or activity is determined by assaying for a cutaneous immunological hypersensitive reaction.

The terms “determining,” “assaying” and “measuring” and grammatical variations thereof are used interchangeably herein and refer to either qualitative or quantitative determinations, or both qualitative and quantitative determinations, that involve manipulation or processing. When the terms are used in reference to measurement or detection, any means of assessing the relative amount, including the various methods set forth herein and known in the art, performed by the hand of man, is contemplated.

The invention provides kits including protein, peptide, subsequence, portion, homologue, variant or derivative thereof (e.g., of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)), combination compositions and pharmaceutical formulations thereof, packaged into suitable packaging material. Kits can be used in various in vitro, ex vivo and in vivo methods and uses, for example a treatment method or use as disclosed herein.

A kit typically includes a label or packaging insert including a description of the components or instructions for use in vitro, in vivo, or ex vivo, of the components therein. A kit can contain a collection of such components, e.g., a protein, peptide, subsequence, portion, homologue, variant or derivative thereof (e.g., of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)), alone, or in combination with another therapeutically useful composition (e.g., an immune modulatory drug).

The term “packaging material” refers to a physical structure housing the components of the kit. The packaging material can maintain the components sterilely, and can be made of material commonly used for such purposes (e.g., paper, corrugated fiber, glass, plastic, foil, ampules, vials, tubes, etc.).

Kits of the invention can include labels or inserts. Labels or inserts include “printed matter,” e.g., paper or cardboard, or separate or affixed to a component, a kit or packing material (e.g., a box), or attached to an ampule, tube or vial containing a kit component. Labels or inserts can additionally include a computer readable medium, such as a disk (e.g., hard disk), optical disk such as CD- or DVD-ROM/RAM, DVD, MP3, magnetic tape, or an electrical storage media such as RAM and ROM or hybrids of these such as magnetic/optical storage media, FLASH media or memory type cards.

Labels or inserts can include identifying information of one or more components therein, dose amounts, clinical pharmacology of the active ingredient(s) including mechanism of action, pharmacokinetics and pharmacodynamics. Labels or inserts can include information identifying manufacturer information, lot numbers, manufacturer location and date.

Labels or inserts can include information on a condition, disorder, disease or symptom for which a kit component may be used. Labels or inserts can include instructions for the clinician or for a subject for using one or more of the kit components in a method, use, treatment protocol or therapeutic regimen. Instructions can include dosage amounts, frequency or duration, and instructions for practicing any of the methods and uses, treatment protocols or therapeutic regimes set forth herein. Exemplary instructions include, instructions for modulating an immune response or activity of a cell against an allergen; modulating an immune response against an allergen in a subject; desensitizing, or inducing, eliciting, increasing or improving immunological tolerance to a protein or peptide allergen; reducing risk or providing a subject protection against an allergic reaction, allergic response, allergic disorder or allergic disease; treating an allergic reaction, allergic response, allergic disorder or allergic disease; or detecting an allergic response or diagnosing an allergy in a subject.

Labels or inserts can include information on any benefit that a component may provide, such as a prophylactic or therapeutic benefit. Labels or inserts can include information on potential adverse side effects, such as warnings to the subject or clinician regarding situations where it would not be appropriate to use a particular composition. Adverse side effects could also occur when the subject has, will be or is currently taking one or more other medications that may be incompatible with the composition, or the subject has, will be or is currently undergoing another treatment protocol or therapeutic regimen which would be incompatible with the composition and, therefore, instructions could include information regarding such incompatibilities.

Invention kits can additionally include other components. Each component of the kit can be enclosed within an individual container and all of the various containers can be within a single package. Invention kits can be designed for cold storage. Invention kits can further be designed to contain to the protein, peptide, subsequence, portion, homologue, variant or derivative thereof (e.g., of an amino acid sequence of a protein or peptide set forth in Table 5 (SEQ ID NOs:1-1,411), Table 6, Table 7 (SEQ ID NOs:1,412-1,906) or Table 8 (SEQ ID NOs:1,907-2,008)), or combination compositions or pharmaceutical compositions.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described herein.

All publications and patent applications cited in this specification are herein incorporated by reference as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the invention is not entitled to antedate such publication by virtue of prior invention.

As used in this specification and the appended claims, the use of an indefinite article or the singular forms “a,” “an” and “the” include plural reference unless the context clearly dictates otherwise. In addition, it should be understood that the individual peptides, proteins, antigens, allergens (referred to collectively as compositions), or groups of compositions, modeled or derived from the various components or combinations of the compositions, and substituents described herein, are disclosed by the application to the same extent as if each composition or group of compositions was set forth individually. Thus, selection of particular peptides, proteins, antigens, allergens, etc. is clearly within the scope of the invention.

As used in this specification and the appended claims, the terms “comprise”, “comprising”, “comprises” and other forms of these terms are intended in the non-limiting inclusive sense, that is, to include particular recited elements or components without excluding any other element or component. Unless defined otherwise all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this invention belongs. As used herein, “about” means + or −5%. The use of the alternative (e.g., “or”) should be understood to mean one, both, or any combination thereof of the alternatives, i.e., “or” can also refer to “and.”

As used in this specification and the appended claims, any concentration range, percentage range, ratio range or other integer range is to be understood to include the value of any integer within the recited range and, when appropriate, fractions thereof (such as one tenth and one hundredth of an integer), unless otherwise indicated. For example, although numerical values are often presented in a range format throughout this document, a range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the use of a range expressly includes all possible subranges, all individual numerical values within that range, and all numerical values or numerical ranges including integers within such ranges and fractions of the values or the integers within ranges unless the context clearly indicates otherwise. This construction applies regardless of the breadth of the range and in all contexts throughout this patent document. Thus, to illustrate, reference to a range of 90-100% includes 91-99%, 92-98%, 93-95%, 91-98%, 91-97%, 91-96%, 91-95%, 91-94%, 91-93%, and so forth. Reference to a range of 90-100%, includes 91%, 92%, 93%, 94%, 95%, 95%, 97%, etc., as well as 91.1%, 91.2%, 91.3%, 91.4%, 91.5%, etc., 92.1%, 92.2%, 92.3%, 92.4%, 92.5%, etc., and so forth. Reference to a range of 5-10, 10-20, 20-30, 30-40, 40-50, 50-75, 75-100, 100-150, and 150-175, includes ranges such as 5-20, 5-30, 5-40, 5-50, 5-75, 5-100, 5-150, 5-171, and 10-30, 10-40, 10-50, 10-75, 10-100, 10-150, 10-175, and 20-40, 20-50, 20-75, 20-100, 20-150, 20-175, and so forth. Further, for example, reference to a series of ranges of 2-72 hours, 2-48 hours, 4-24 hours, 4-18 hours and 6-12 hours, includes ranges of 2-6 hours, 2, 12 hours, 2-18 hours, 2-24 hours, etc., and 4-27 hours, 4-48 hours, 4-6 hours, etc.

Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it is readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims. The invention is further exemplified by way of the following non-limited examples.

EXAMPLES Example 1

This example includes a description of various materials and methods.

Patient Donor Population:

Patient recruitment for this study was performed at the University of California, San Diego (UCSD), and at National Jewish Health (NJH) in Denver, Colo., under each local IRB approved protocol, as well as LIAI Institutional Review Board IRB approved protocol VD1-059-0311 (Federal Wide Assurance #00000032). Informed consent, study ID numbers, clinical case histories and other information were collected and recorded by clinical investigators. Immediate hypersensitivity skin test reactivity to a panel of extracts from 28 common allergens, as well as positive (histamine), and negative controls (diluent), was determined by standard methods. Both wheal (mm) and flare (mm) were measured at 15 minutes. All volunteers were asked to provide a 5 ml serum sample and a unit of peripheral blood.

An allergic donor was defined based on a history of allergic rhinitis and a positive skin test (a wheal of at least 3 mm in diameter greater than the diluent negative control) to one or more of the allergens tested. A total of 87 allergic donors were investigated. The donor cohort included 45 females and 42 males, and ranged between 20-63 years of age. Of the 87 donors, 52 had rhinitis and another 29 were categorized as having rhinitis and asthma (6 were not classified).

The main goal was to identify a majority of the epitopes that are frequently recognized in the human population. By analyzing at least ten donors for each allergen of interest, the sample size is sufficient such that 80% of all epitopes that are recognized in 15% or more allergic individuals in the general population are expected to give a positive response in one or more of the ten individuals in our study (assuming a binomial distribution). Similarly, 95% of all epitopes recognized in 40% of the allergic population are expected to give responses in two or more individuals in the study. Based on these considerations, studying ten donors per allergen is sufficient to identify targets of frequent responses.

Bioinformatic Analyses:

Uniprot accession IDs for each of a panel of 28 common allergens were collected from the IUIS Allergen Nomenclature database, then used to retrieve sequences from the Uniprot database (Table 5, SEQ ID NOs:1-1,411). For those allergens without Uniprot IDs, we used the sequences provided by IUIS. If an allergen (e.g. Alt al) had multiple sequences, a representative sequence for each allergen was selected. The representative sequence should have the longest length and greatest sequence coverage (or sequence identity >90%) compared to other sequences. If the sequence identity between two sequences that belong to the same allergen is <40%, both sequences were selected. As a result, 169 non-redundant sequences were identified.

Sequences, including isoforms, were then scanned for unique 15-mer peptides overlapping by 10 residues. Each peptide was then predicted for its capacity to bind to a panel of 20 of the most common HLA class II alleles (DPA1*0103/DPB1*0201, DPA1*0201/DPB1*0101, DPA1*0201/DPB1*0501, DPA1*0301/DPB1*0402, DQA1*0101/DQB1*0501, DQA1*0301/DQB1*0302, DQA1*0401/DQB1*0402, DQA1*0501/DQB1*0301, DRB1*0101, DRB1*0301, DRB1*0401, DRB1*0405, DRB1*0701, DRB1*0802, DRB1*1101, DRB1*1302, DRB1*1501, DRB3*0101, DRB4*0101, DRB5*0101) using the consensus prediction described in (19). Peptides with predicted binding scores in the top 20% for a given allele were considered potential binders, and the number of HLA molecules each peptide was predicted to bind was enumerated. All peptides predicted to bind 10 or more HLA molecules were selected for synthesis and further study.

To make sure each allergen is adequately represented in the peptide set, at least 2 peptides were included for each allergen. It was further stipulated that these 2 peptides should not be located in the first 20 residues (which likely contain signal sequences), and that the 2 peptides should not directly overlap (e.g. peptide 56-70 and 61-75). These additional requirements could be met for all but 4 proteins (Sec c 1, Sec c 20, Ant o 1, Dac g 2) for which only fragmentary sequence information was available. The complete list of Peptides is shown in Table 5.

Peptide Synthesis:

Peptides for screening studies were purchased from Mimotopes (Clayton, Victoria, Australia) and/or A and A (San Diego, Calif.) as crude material on a small (1 mg) scale. Peptides utilized as radiolabeled ligands were synthesized on larger scale, and purified (>95%) by reversed phase HPLC.

HLA Binding Assays:

Assays to quantitatively measure peptide binding to MHC class II molecules, based on the inhibition of binding of a high affinity radiolabeled peptide to purified MHC molecules, have been described in detail elsewhere (20). Briefly, MHC molecules were purified from EBV transformed homozygous cell lines by monoclonal Ab-based affinity chromatography. HLA-DR, DQ and DP molecules were captured by repeated passage of lysates over LB3.1 (anti-HLA-DR), SPV-L3 (anti-HLA-DQ) and B7/21 (anti-HLA-DP) columns.

For inhibition experiments, 0.1-1 nM of radiolabeled peptide was co-incubated at room temperature or 37° C. with 1 μM to 1 nM of purified MHC in the presence of a cocktail of protease inhibitors and various amounts of inhibitor peptide. Following a 2 to 4 day incubation, the percent of MHC bound radioactivity was determined by capturing MHC/peptide complexes on LB3.1 (DR), L243 (DR), HB180 (DR/DQ/DP), SPV-L3 (DQ) or B7/21 (DP) Ab coated Optiplates (Packard Instrument Co., Meriden, Conn.), and bound cpm measured using the TopCount (Packard Instrument Co.) microscintillation counter. Inhibitor peptides were tested in at least three or more independent assays at six different concentrations covering a 100,00-fold dose range. Under the conditions utilized, where [label]<[MHC] and IC50≧[MHC], the measured IC50 values are reasonable approximations of the true Kd values (21, 22).

PBMC Isolation and HLA Typing:

PBMC were obtained by density gradient centrifugation (Ficoll-Hypaque, Amerhsam Biosiences, Uppsala, Sweden) from one unit of blood (450 ml), according to manufacturer's instructions, and cryo-preserved for further analysis. An aliquot of serum was obtained for RAST IgE and IgG analyses (performed at NJMRC, Denver, Colo. & Phadia).

HLA typing was performed according to standard methods. Briefly, genomic DNA isolated from PBMC of the study subjects by standard techniques (QIAmp, Qiagen, Valencia, Calif.) was used for HLA typing. High resolution Luminex-based Sequence-Specific Oligonucleotide (SSO) typing for HLA Class I and Class II was utilized according the manufacturer's instructions (One Lambda, Canoga Park, Calif.). Where needed, PCR based Sequence-Specific Primer (SSP) typing methods were used to provide high resolution sub-typing (One Lambda, Canoga Park, Calif.).

In Vitro Expansion of Allergen-Specific T Cells:

PBMCs were cultured in RPMI 1640 (V Scientific, Tarzana, Calif.) supplemented with 5% human serum (Cellgro, Herndon, Va.) at a density of 2×106 cells/ml in 24-well plates (BD Biosciences, San Jose, Calif.) and stimulated with 2 to 50 μg/ml of allergen extract (Greer, Lenoir, N.C.) depending on the allergen (Table 6). Cells were kept at 37° C. in 5% CO2 and additional IL-2 (10 U/ml; eBioscience, San Diego, Calif.) was added every 3 days after initial antigenic stimulation. On day 14, cells were harvested and screened for reactivity against the allergen-specific peptide pools or individual peptides. LPS content of the various extracts was measured by Indoor Biotechnologies (Charlottesville, Va.) using standard Limulus Amebocyte Lysate (LAL) methodology.

ELISPOT Assays:

The production of IL-5 and IFN-γ was analyzed in ELISPOT assays. Flat-bottom 96-well nitrocellulose plates (Millipore, Bedford, Mass.) were prepared according to manufacturer's instructions and coated with 10 μg/ml anti-human IL-5 (Clone TRFK5; Mabtech, Cinncinati, Ohio) and anti-human IFN-γ (Clone 1-D1K; Mabtech, Cincinnati, Ohio). Cells were then incubated at a density of 1×105/well either with peptide pools or individual peptides (10 μg/ml), extract (2-50 μg/ml), PHA (10 μg/ml), or medium containing 0.1% DMSO (corresponding to the percentage of DMSO in the pools/peptides) as a control. After 24 hours, cells were removed, and plates were incubated with either 2 μg/ml biotinylated anti-human IL-5 Ab (Mabtech) and 1:200 HRP-conjugated antihuman IFN-γ Ab (Mabtech) at 37° C. After 2 hours, spots corresponding to the biotinylated Abs (IL-5) were developed by incubation with Alkaline Phosphatase-Complex (Vector Laboratories, Burlingame, Calif.) followed by incubation with Vector Blue Alkaline Phosphatase Substrate Kit III (Vector Laboratories) according to the manufacturer's instructions. Spots corresponding to the HRP-conjugated Ab (IFN-γ) were developed with 3-amino-9-ethylcarbazole solution (Sigma-Aldrich, St. Louis, Mo.). Spots were counted by computer-assisted image analysis (Zeiss, KS-ELISPOT reader, Munich, Germany).

Each assay was performed in triplicate. The level of statistical significance was determined with a Student's t-test using the mean of triplicate values of the response against relevant pools or individual peptides versus the response against the DMSO control. Criteria for peptide pool positivity were 100 spot-forming cells (SFCs)/106 PBMC, p≦0.05 and a stimulation index (SI)≧2, while criteria for individual peptide positivity were ≧20 SFC/106 PBMC, p≦0.05, and a SI≧2. The SFC/10̂6 criteria utilized (in conjunction with also passing a T test with p<0.05, and a SI>2) have been used in several recent studies from our group (see, e.g., 16-19, 31-36). In particular, in the context of allergen epitope identification, a recent study analyzing responses to Timothy Grass allergens validated much of the methodology applied in the present study. Together, in these studies it was also noted that, in general, epitope pools yielding significant but relatively weaker responses (in the 20 to 100 SFC range) did not lead to the identification of significant and consistent responses at the level of individual peptides. For this reason, and because cells are often limiting, and pool deconvolution is the most demanding step in terms of cell requirement, in those studies, as well as in the present study, only pools yielding 100 SFC/10̂6 were deconvoluted.

HLA Restriction:

To determine the HLA locus restriction of identified epitopes, mAb inhibition assays were performed as described previously (11, 37). Preliminary determinations were made on control T cell clones of known specificity to determine optimal antibody doses leading to complete inhibition of the specific clones, and not associated with inhibition of clones known to be restricted by a different HLA allele or locus. This antibody concentration was then utilized in experiments where a dose response of antigenic peptide was tested in the presence or absence of the specific antibodies. Experimental determinations were performed utilizing ELISPOT assays specific for the particular lymphokine utilized to originally identify the particular epitope mapped.

For each antigenic region/donor combination short-term T cell lines were derived by extract stimulation of triplicate cultures of 2-3 million cells. IL-2 was added 5-8 days following stimulation. After 14 days of stimulation with the corresponding allergen extract (2-50 μg/ml), the HLA locus that restricted the response to the specific lymphokine was determined by measuring the capacity of mAbs specific for HLA-DR, DP or DQ to inhibit (block) the response. For this, PBMCs were incubated with 10 μg/ml of mAbs (Strategic Biosolutions, Windham, Me.) against HLA-DR (LB3.1), DP (B7/21) or DQ (SVPL3) 30 minutes prior to addition of 10 μg/ml of peptide. Cytokine production induced by positive peptides was then measured in ELISPOT assays as described above. The pan MHC class I Ab (W6/32) was used as a control. The decrease (inhibition) in cytokine production in the presence of an HLA locus specific mAb, relative to production in the absence of mAb, was determined. A response was considered as restricted by HLA alleles at a specific locus when ≧50% inhibition of the response was observed in the presence of the corresponding mAb.

Example 2

This example includes a description of the identification of 257 different antigenic regions from common allergen sources.

T cell responses to complex allergens in humans are very heterogeneous and involve recognition of a large number of epitopes (3-18). Recent work demonstrated that the most dominant and prevalent responses could be predicted on the basis of their capacity to bind multiple HLA class II molecules. Here, this approach was used to investigate a large panel of allergen proteins derived from 28 different allergen sources.

This study broadly addressed inhalants and contact allergens, including allergens derived from fungi (Alternaria, Aspergillus, Cladosporuim and Penicillium), trees (Alder, Ash, Birch, Black Walnut, Cypress, Juniper, Oak and Palm), grasses (Bermuda, Canary, Kentucky Blue, Orchard, Rye and Sweet Vernal), weeds (English Plantain, Giant Ragweed, Mugwort, Russian Thistle, and Western Ragweed) and various indoor allergens (American Cockroach, Cat dander, Dog dander and Dust Mites) (Table 1). These allergen sources were selected because of their common diagnosis by extract reactivity in allergic patients at the two participating clinical sites, and availability in the IUIS database of at least some allergen protein sequences (a complete list of these allergen proteins is provided in Table 5 (SEQ ID NOs:1-1,411). The strategy was utilized to predict potential allergen derived T cell epitopes.

In the studies, In vitro stimulation for a 14-17 day period was utilized. As shown in FIG. 1, additional data from three individual donors for two different allergen sources (Alternaria and Rye Grass) demonstrate that both IFN-γ and IL-5 responses are optimally detected in the 14-17 day window. The doses of individual extracts used for In vitro stimulation were chosen on the basis of initial dose titration experiments. For each extract the magnitude of responses, as well as cell viability at the end of the In vitro stimulation step, were noted. For certain extracts toxicity (loss of viability) was observed. In all studies highest dose not associated with noticeable toxicity was used. The exact dose utilized for each extract source is shown in Table 6.

As described in the Materials and Methods, peptides ranking in the top 20% of predicted affinities for 10 or more of 20 common HLA class II alleles were selected for synthesis and further analysis. In total (see Table 1), 133 different proteins were analyzed, and a total of 1411 predicted promiscuous binders were synthesized, corresponding to an average of 10.6 peptides per protein. On average, about 50 peptides were made for each allergen source, with a range of 3 to 229 (a list of the peptides synthesized is also provided in Table 5 (SEQ ID NOs:1-1,411).

Short-term lines stimulated with extract, as described above, were tested with pools of 15-20 peptides from the proteins of the corresponding allergen, and then individual epitopes were identified by deconvolution of positive pools (11). The dose of peptide utilized in the experiments was selected based on the fact that for pools of 20 peptides, in which the initial individual peptide stocks are 40 mg/ml in 100% DMSO, the highest final concentration of each individual peptide that can be achieved, without reaching a total pool DMSO concentration that is toxic in the assay (0.25-0.5%), is 4-5 μg/ml. A dose of 10 μg/ml was used for experiments with single peptide stimulations. This is consistent with our experience in other systems (see, e.g., 11, 17, 21, 31, 33, 38), and is also a dose routinely used in the literature relating to stimulation of human class II restricted T cells.

An additional issue to be addressed is whether different epitopes may be recognized on day 6 compared to day 14 of cultures. To address this issue, the pattern of epitopes identified was compared for two representative donors (D00095 and D00107) in the Alternaria system. The data, shown in FIG. 2, illustrate how the epitopes identified on day 6 were also identified on day 14. In addition, because of the stronger signal obtained on day 14, additional peptides that would have been missed on day 6 are revealed at the day 14 time-point.

Different lymphokines are differentially produced and regulated (39). However, in our experience, the epitopes that are recognized by IL-5 producing cells are essentially the same ones recognized by IL-4/IL-13 producing cells. Furthermore, IL-5 and IL-4/IL-13 producing cells are largely overlapping. Accordingly, the present study focused on IL-5 as a representative lymphokine of the Th2 lineage. To illustrate the rationale, FIG. 3 summarizes an analysis of IL-4, IL-5 and IL-13 producing cells in representative donors in response to Alternaria and Rye Grass peptides. These data demonstrate that, indeed, the epitopes recognized by IL-5 producing cells are the same ones recognized by IL-4/IL-13 producing cells. Furthermore, the data show that IL-5 and IL-4/IL-13 producing cells are largely overlapping and that there is little to gain by measuring all three lymphokines. Thus, while measuring all three lymphokines separately would be of obvious interest, it essentially doubles the number of cells required, and thus would not be easily compatible with the large number of allergens studied, and the relatively high-throughput nature of the assay strategy utilized in the present study.

PBMC from donors with positive skin tests to the allergen in question were restimulated In vitro with the various corresponding allergen extracts. Allergen extract stimulation was effective in most of the donors (94% overall, and on average across all sources; range 67 to 100% for the different extracts; Table 6). These short-term, extract-stimulated, lines were tested with pools of 15-20 peptides from the proteins of the corresponding allergen, and then individual epitopes were identified by deconvolution of positive pools (11). For each allergen source, we tested PBMCs from at least ten different allergic donors (range 10-15, average 11), as determined by skin test to the corresponding allergen. in standard dual ELISPOT assays detecting IFN-γ and IL-5.

Although this may be considered to be a limited number of donors, this number was chosen to enable identification of epitopes that are most frequently recognized in the donor population, consistent with the promiscuous HLA binding principle used to select the candidate epitopes. According to power calculations, by studying the response in 10 allergic donors, 80% of all epitopes that are recognized in 15% or more allergic individuals in the general population would be identified (assuming a binomial distribution). Similarly, 95% of all epitopes recognized in 40% of the allergic population are expected to give responses in two or more individuals in our study. This is consistent with the scope of the current investigation, which was to survey a large number of allergen sources, side-by-side, utilizing the same experimental design, and characterizing the most dominant responses.

In total, 322 peptides were positive in at least one donor. ELISPOT results from each individual peptide are presented in Table 7 (SEQ ID NOs:1,412-1,906). The high rate at which predicted peptides were found to be antigenic (322/1405=22.9%) demonstrates the power of the approach based on prediction of promiscuous HLA class II binding. Some epitopes were highly homologous because they were derived from allergen isoforms, or from the same allergen protein and covered largely overlapping regions. After removal of redundancies and consolidation of largely overlapping regions, a total of 289 unique epitopes, corresponding to 257 distinct antigenic regions, were identified (Table 1).

Example 3

This example includes a description of the allergen epitopes identified.

The sequences of the 257 antigenic regions identified were compared to the known human Class II/CD4 epitopes curated in the IEDB (www.iedb.org; (23)). To obtain a data set with characteristics comparable to the one obtained in the screening described herein, the IEDB was queried for all epitopes defined in human hosts, either ex vivo or by utilizing short-term lines, and for which CD4/class II restriction could either be demonstrated or inferred on the basis of the assay methodology. This analysis determined the extent to which the identified epitopes were novel or previously cited.

Overall, epitopes were identified for 25 of the 28 allergen sources studied. For 14 of the allergen sources, no epitope could be found in the IEDB that satisfied the criteria defined above for any of the epitopes identified for the allergen (Table 2A). By contrast, and as expected, in the cases of 11 other allergen sources which have been previously and more extensively studied, many of the epitopes identified mapped to regions reported as being antigenic in allergic patients (Table 2B). Nonetheless, in those cases, 84% of the epitopes identified had not been previously reported.

Conversely, only 38% of the epitopes already curated by the IEDB were re-identified by the present study. This is consistent with the results obtained in a Timothy Grass study, which estimated that the predictive approach would identify the most dominant and prevalently recognized epitopes, corresponding to about 50% of the total T cell response (3). Thus, the analysis presented in this section underscores the novel nature of a large number of the epitopes identified in the current study.

Example 4

This example includes an analysis of the distribution of epitopes identified as a function of the various allergen sources.

As mentioned above, allergen extract stimulation was effective in 95% of the donors. However, in some cases a relatively large number of epitopes were identified, while in other cases the screen revealed no, or only a few, epitopes. To quantitatively express these variations, for each allergen source the ratio of the total epitope specific IL-5 and IFNγ response to the total response observed against the corresponding crude allergen extract was calculated (Table 3).

In 16 cases the peptide specific responses corresponded to 15% or more of the response detected against the extract, while in 12 cases the peptide responses totaled less than this arbitrary threshold. Indeed, the 16 allergen sources associated with the larger total peptide responses accounted for 230 of the 257 (89%) epitope regions identified, and 74 of the 81 (91%) regions recognized by 2 or more donors (see also Table 7, SEQ ID NOs:1,412-1,906).

When the number of known allergenic proteins described in the IUIS database, and utilized for the present analysis, was scrutinized (Table 3), a correlation between the fraction of the extract response that could be attributed to the peptides studied, and the number of representative protein sequences available, became apparent. In other words, the sources for which the set of T cell epitopes identified accounted for only a minor fraction of the extract response were represented by fewer protein sequences (median 1.5) as compared to the sources where the epitopes accounted for a larger fraction of the extract response (median 5 protein sequences, RS=0.49; p=0.01). These results suggest that, in the cases of the “low epitope coverage” allergen sources, the number of antigenic T cell targets was too limited to allow for capture of the complexity of responses. Thus, it is likely that for these sources additional allergens exist.

Exceptions to this trend were noted, however. For example, in the case of White Oak, only one protein was represented in the IUIS. However, peptides from that one protein accounted for almost 50% of the extract response. Other instances where an appreciable fraction (21-47%) of the extract response was associated with just a few proteins were also noted. Indeed, as shown in Table 3, for 5 of the 16 allergens for which >15% of the extract response was identified with antigen specific peptide pools, only one or two proteins were available for study. These data suggest that for these allergen sources the major T cell epitopes are derived from a limited number of proteins. Conversely, it is remarkable that for some organisms, such as Aspergillus, where many proteins were scanned (n=23), only a few (n=1) were found to have epitopes. Finally, in some cases, such as American Cockroach, the paucity of epitopes might be reflective of the weak level of sensitization of the particular patient population investigated in this study, as judged by low skin test reactivity.

Example 5

This example includes a description of promiscuous restriction of dominant epitopes.

The next series of studies was focused on 74 epitope regions that were recognized in two or more donors and derived from the 16 allergen sources in which peptide specific responses could account for 15% or more of the responses detected with the extract. These more prominent epitopic regions, listed in Table 4, accounted for 70% of the total SFC response detected against all of the epitope regions identified from these 16 different allergen sources.

Each of the individual 15-mer peptides associated with these regions was tested for its capacity to bind a panel of 35 different DR, DP and DQ molecules representative of the most common allelic variants worldwide (24-26), including all 20 molecules comprising the prediction panel. It was found that 73% of the epitopes bound 50% or more of the molecules tested with an affinity of 1000 nM, or better (FIG. 4; binding data for each peptide is presented in Table 8, SEQ ID NOs:1,907-2,008). By contrast, in a control set of a panel of 425 unbiased, non-redundant, peptides representing 15-mers, overlapping by 10 residues, spanning the entire sequences of the P. pratense 1, 2, 3, 4, 5, 6, 7, 11, 12, and 13 pollen antigens, only 17% bound 50% or more of the same molecules. As the allergen-derived peptides tested herein were selected on the basis of predicted promiscuous HLA class II binding capacity, overall, these data support the validity of the predictive approach for identification of promiscuous binding peptides.

Based on the predictive approach taken and the data presented above, it was expected that a diverse set of HLA allelic variants would be restricting the T cell responses to the epitopes identified. To address this issue experimentally, the HLA locus restriction of the more frequently recognized epitopes was determined by inhibition experiments utilizing DR-, DP- and DQspecific antibodies. Locus restriction could be determined for a total of 65 antigenic regions (Table 4). In the remaining combinations (about a third of the cases), locus restriction could not be determined, either due to a scarcity of cells, low responses, or because 50% inhibition by locus specific mAb could not be achieved, perhaps reflective of promiscuous locus restriction at the level of individual donors.

Multiple restricting loci were observed in about a third of the cases (21/65; 32.3%). Furthermore, in 10 of the 17 (59%) cases where a single locus was indicated by the antibody inhibition experiments as restricting a specific epitope in multiple donors, no single allelic variant capable of binding the epitope In vitro was shared by all of the responding donors, thus implying intra-locus promiscuous restriction as well. Overall, the data presented in this section highlights the promiscuous restriction of the more prevalent and dominant epitopes identified.

Example 6

This example includes a description of studies showing that T cell responses to different allergen sources are differentially polarized.

As described herein, the epitope specific responses for the various allergen sources were determined by utilizing both IL-5 and IFN-γ ELISPOT assays. These lymphokines were chosen as prototype Th2 and Th1 responses, respectively. For each of the allergen sources, next the relative proportion of the response attributed to each of these two lymphokines was inspected. As expected, overall IL-5 production exceeded IFN-g production by a ratio of approximately 2:1. Interestingly, however, different patterns were noted for individual allergen sources (FIG. 5). In some cases (e.g., Orchard grass, Alternaria, Cypress, and Russian Thistle), IL-5 production exceeded IFN-g production by 5-10-fold. In other cases (e.g., Aspergillus, Penicillum, and Alder), the converse was noted, as production of IFN-g exceed IL-5 production by more than 3-10-fold. These results suggest that different allergen sources are associated with different degrees of polarization of the responding T cell subsets. It was hypothesized that differential polarization may correlate with the skin test response, such that a late phase skin response may associate more frequently with the Th1-type allergen/epitope. However, examination of the data revealed no discernable trend (FIG. 5).

Similarly, it was hypothesized that the polarization observed was due to the different allergenic extracts having different levels of LPS content. When the LPS content of the various extracts was measured (see the table embedded in FIG. 5) there was no correlation (r=0.14, −0.16 and −0.16 for IFNγ, IL-5 and the IFNγ/IL-5 ratio, respectively) between the LPS content the Th1/Th2 skewing we observed.

Strikingly, even within an individual donor, responses to different allergen sources could be differentially polarized, with responses to one allergen dominated by Th1 responses, and to a different allergen dominated by Th2 responses. An example of this type of situation is shown in FIG. 6, which depicts the T cell responses observed in a donor who responded to epitopes from Alder and Alternaria rot fungus, allergens associated with Th1 and Th2 polarization, respectively. As shown, the response by this donor to Alder (Aln g) was completely Th1, while the response to Alternaria (Alt a) was completely Th2.

Example 7

This example includes a description of methods of determining in vivo efficacy of proteins and peptides of the invention, in particular, for treatment of allergy.

Proteins and peptides of the invention are evaluated for efficacy in treatment of allergy in a mouse model. Six groups of BALB/cJ or HLA-transgenic mice are sensitized with repeat dosing of 1.5 micrograms of each whole allergen intranasally (in 25 uL) for 5×2 days over 2 weeks. This serves as a model system for investigation of allergic asthma caused by each whole allergen.

The sensitized mice are then left for one week before treatment with peptides of the invention. The treatment comprises intranasal delivery of allergen peptides followed 30 minutes later by intranasal delivery of allergen peptides daily for 5 days. Approximately 4 weeks later the mice are challenged with each whole allergen for 2 days (2×15 ug/25 uL intranasally) and outcomes are measured 48 hours later. 5 doses of allergen peptides are evaluated (10 μl, 0.1, 0.01 & 0.001 ug per peptide). Appropriate control experiments are conducted.

The outcomes measured are bronchial airway resistance following methacholine lung challenge (cm H20/mL/s), a measure of respiratory function, and a quantitation of inflammatory cells in the bronchioalveolar lavage (BAL) fluid.

For measurement of airway resistance, 48 hours after intranasal challenge over 2 days (2×15 ug) with each whole allergen, total respiratory system resistance (Rrs) is measured in response to intranasal saline and increasing doses of intravenous methacholine (MCh) using the Flexivent rodent ventilator. Using the resulting Rrs-MCh dose-response curves, indices of airway reactivity (Slope Rrs) and maximal degree of bronchoconstriction at 25 MCh mg/mL (Max Rrs @ 25 mg/mL) are measured. Values are means+/−SE.

For quantitation of inflammatory cells, bronchioalveolar lavage fluid (BALF) is assessed for total and differential inflammatory cell counts. Sections of lung tissue are stained with hematoxylin and eosin (H&E) and morphometrically quantified using a custom computerized analysis system (Northern Eclipse).

Example 8

This example includes a description of a clinical trial protocol of proteins and peptides of the invention for treatment of allergy.

Proteins and peptides of the invention are analyzed in a randomized, placebo-controlled, blind clinical trial for efficacy in reducing allergic symptoms. The study design of the clinical trial is in accordance with good clinical practice guidelines.

Baseline skin responses to each allergen for all subjects are established using a Baseline Challenge between 6 and 8 days prior to study medication administration. Two intradermal injections of 0.010 HEP (histamine equivalent prick) units of commercially available standard allergen is administered, separated by a 30 minute time interval, into the volar surface of the left and right forearms respectively. Subjects are assessed to ensure that they experience a Late-Phase Skin Response (LPSR) to each whole allergen, and the magnitude of the baseline reaction is recorded as follows:

Eight hours after each injection the outline of any late-phase response is drawn onto the skin with a ballpoint pen. The longest and orthogonal diameters are measured and recorded for each response, and the area of the response in each arm is calculated. The average area of response in both arms of each subject is then calculated to provide the baseline reaction. Subjects who produced a suitable baseline reaction are assigned to dosing groups, randomized and entered into the Treatment Phase.

The Treatment Phase consists of a period of 21 days for each subject. During this period one group of subjects receives a single intradermal injection of either peptides of the invention (0.03, 0.3, 3, 12 nmol of each peptide per dose) or diluent placebo at Treatment Phase Visit 1 on day one. A cohort of 8 subjects receives treatment at each dose level (6 receives the peptides of the invention and 2 placebo). The first cohort of the intradermal group receives 0.03 nmol of each peptide in the mixture and each subsequent cohort in the group receives the next higher dose level.

Intradermal injections are made into the flexor surface of the left forearm. The total volume of the injection is 60 μL for all injections. After treatment, subjects have their skin response to whole allergen retested at Treatment Phase Visit 2 on day 21 (±3 days). Skin responses to whole allergen are assessed by measurement of the late-phase responses 8 hours following intradermal administration of 0.010 HEP (histamine equivalent prick) units of commercially available standard allergen as described above. The average area of response for both arms of each subject is then calculated as described above.

This average LPSR area after treatment is then compared to the baseline LPSR area for each subject. The overall change in LPSR area for all eight patients in each cohort is then evaluated.

Example 9

This example includes a discussion of the data.

Disclosed herein are results of a systematic survey of T cell epitopes derived from common allergens. This is believed to be the first such study simultaneously investigating 28 different common allergen sources with the same controlled and uniform technical approach. This approach is designed to allow relatively high throughput analysis, while still capturing a large fraction of the total class II restricted allergen specific T cell response, and has been validated in Timothy Grass (3) and German Cockroach systems. The approach is based on prediction of the peptides most likely to bind a panel of HLA molecules chosen to be representative of the most common HLA class II allelic variants at the DR, DP and DQ loci.

Each peptide was tested in at least ten donors specifically allergic to each allergen source. It was reasoned that this number of donors would be suitable to identify the more dominant, and more frequently recognized, epitopes, while at the same time allowing screening for a broad and diverse set of allergen sources. Nearly a quarter (322/1405=22.9%) of the predicted peptides were positive in at least one of the donors tested, thus further validating the approach, and illustrating its broad applicability to human inhalant and contact allergens, including fungus, tree, grass, weed and animal allergens.

The significance of the observations is highlighted by the comparison of these newly identified epitopes with those previously identified and described in the scientific literature and curated in the IEDB (23, 27). For 14 of the allergen sources, no human class II/CD4 epitope could be found in the IEDB that overlapped with any of the epitopes described herein. For 11 other allergen sources, which have been more extensively studied, no human class II/CD4 epitope could be found in the IEDB that overlapped with 84% of the epitopes identified. Conversely, when the data was analyzed to pinpoint the fraction of IEDB-contained epitopes that were re-identified in the course of the present study, it was found that, on average, 38% of the known epitopes were re-identified. This is consistent with the Timothy Grass and German Cockroach studies noted above, which estimated that the predictive approach would identify the most dominant and prevalently recognized epitopes, corresponding to about 50% of the total T cell response.

The epitopes most frequently recognized were characterized in terms of their HLA binding capacity to a panel of 35 different HLA class II molecules. This panel was chosen to be representative of the most common allelic variants in the general population at the HLA class II DR, DP and DQ loci (24-26). This data is of relevance, as the quantitative binding data to the various common HLA class II variants can be used to project the potential coverage by the various epitopes in patient populations of different ethnicities (26, 28, 29). The issue of restriction was addressed in experiments where the HLA class II restricting locus was determined by antibody inhibition experiments. It was found that about a third of the epitopes were restricted by multiple loci. Furthermore, it is estimated that at least 60% of the remaining epitopes are restricted by multiple allelic variants at a given locus. These results are not unexpected, given the fact that predicted promiscuous binding was utilized as a selection criterion for identifying candidate epitopes. Nonetheless, these observations underline the relevance of promiscuous epitope recognition in the context of HLA class II allergen-specific T cell responses.

A notable result of the side-by-side survey is that the identified epitopes account for a variable fraction of the response to a given allergen source, and that this fraction further correlates with how many different allergenic proteins have been described for that source. Thus, this data suggests that, at the T cell level, allergic responses target a relatively large number of antigens. Without being limited to any particular theory, future experiments may address whether this observation reflects the existence, for the allergen sources where only one or few specific sequences have been reported, of additional allergens recognized by IgE, and not yet identified. Alternatively, this may also reflect an incomplete overlap between the targets of T cell and IgE responses.

While some studies show a correlation between IL-5 levels and IgE (47), other studies do not (48). Thus, IgE or wheal responses may or may not be related to Th2 (i.e. IL-5) responses, and in our study these appear to depend upon the allergen studied. It was also observed that different allergen sources appear to elicit patterns of responses that are differentially polarized in terms of their Th1/Th2 balance, at least as judged by IL-5 (Th2) or IFN-γ (Th1) production. Strikingly, even within an individual donor, responses to different allergens could be differentially polarized, with responses to one allergen dominated by Th1 responses, and to a different allergen dominated by Th2 responses.

A similar phenomenon was observed with different allergenic proteins in the Timothy Grass system (11) following restimulation with pollen extract. A comparison of the LPS content of the various extracts used with the associated Th1/Th2 balances revealed no correlation, suggesting that the differential polarization observed is antigen specific, and not due to differential LPS content in the various extracts. The molecular basis for this effect is presently unclear and without being limited to any particular theory, might reflect differences in the relative concentrations and accessibility of the different allergens in the pollen and extract, their processing and presentation, and potentially the presence of distinct co-stimulatory signals associated with each allergen. The study of this mechanism might suggest avenues to influence or alter the lymphokine balance of Th responses, and thus potentially the outcome of responses in terms of IgE titers.

The epitopes identified herein are of use in several respects. First, the epitopes of the invention may be used to characterize T cell responses associated with allergic reactions, including characterization of changes in responding T cell phenotype/T cell plasticity as a function of seasonality, as a result of SIT treatment, or as a function of varying disease severity (asthma versus rhinitis). Secondly, T cell epitopes of the invention can be used to develop immunotherapeutic SIT treatments that could target T cell responses without the risks connected to administration of whole allergens capable of binding IgE, and that thus pose potential safety risks.

TABLE 1 Identification of 257 epitope regions from common allergen sources Number of Anti- Allergen Number of peptides Unique genic category Allergen Source allergens tested epitopes regions Fungi Alternaria Rot 10 88 29 25 Fungus A. fumigatus 23 229 11 11 C. herbarum 6 53 15 15 P. chrysogenum 2 38 18 18 Grasses Bermuda Grass 5 21 3 3 Canary Grass 2 20 9 7 Kentucky Blue 2 31 12 9 Grass Orchard Grass 4 15 5 4 Rye Grass 6 70 34 30 Sweet Vernal Grass 5 3 3 2 Indoor American 5 79 3 3 allergens Cockroach Cat epithelia 4 55 10 8 D. farinae 11 160 23 22 D. pteronyssinus 14 224 40 35 Dog epithelia 4 44 5 5 Trees Alder 2 11 4 3 Ash 1 6 3 3 Birch 6 47 14 13 Black Walnut 2 22 0 0 Cypress 5 65 16 12 Date Palm 1 5 3 3 Prickly Juniper 1 3 3 3 White Oak 1 5 4 2 Weeds English Plantain 1 3 2 2 Giant Ragweed 1 5 4 3 Mugwort 4 22 0 0 Russian Thistle 4 83 16 16 Western Ragweed 1 0 0 Total 133 1411 289 257

TABLE 2 Novelty of the epitopes identified A. Novel sources Regions identified Source This study IEDB Novel Alternaria Rot 25 0 25 Fungus P. chrysogenum 18 0 18 Russian Thistle 16 0 16 C. herbarum 15 0 15 Canary Grass 7 0 7 Orchard Grass 4 0 4 American 3 0 3 Cockroach Ash 3 0 3 Prickly Juniper 3 0 3 Date Palm 3 0 3 Giant Ragweed 3 0 3 White Oak 2 0 2 Sweet Vernal 2 0 2 Grass English Plantain 2 0 2 Total 106 0 106 B. Sources for which epitopes were previously described % Regions identified IEDB This Over- % reiden- Source study IEDB lap Novel Novel tified Alder 3 3 2 1 33.3 66.7 D. farina 22 7 2 20 90.9 28.6 A. fumigatus 11 3 1 10 90.9 33.3 Bermuda Grass 3 5 0 3 100.0 0.0 Birch 13 7 3 10 76.9 42.9 Cat epithelia 8 4 4 4 50.0 100.0 Dog epithelia 5 5 0 5 100.0 0.0 D. pteronyssinus 35 8 5 30 85.7 62.5 Japanese Cypress 12 10 3 9 75.0 30.0 Kentucky 9 7 2 7 77.8 28.6 Blue Grass Rye Grass 30 4 2 28 93.3 50.0 Total 151 63 24 127 84.1 38.1

TABLE 3 Heterogeneity in epitope coverage of the different allergen sources Response (SFC) % Peptide/ IUIS Allergen Source Regions Peptide Extract Extract Proteins Rye Grass 30 29722 19437 152.9 4 D. pteronyssinus 35 28129 22505 125.0 14 D. farinae 22 9402 9707 96.9 11 Alternaria Rot Fungus 25 31162 35207 88.5 10 Cypress 12 14310 18160 78.8 5 Cat epithelia 8 20068 28607 70.2 5 Birch 13 9690 17110 56.6 6 White Oak 2 8520 18067 47.2 1 Canary Grass 7 8217 22054 37.3 2 P. chrysogenum 18 9723 26643 36.5 2 Russian Thistle 16 11848 32723 36.2 5 C. herbarum 15 9200 31020 29.7 6 Kentucky Blue Grass 9 11007 39232 28.1 7 Alder 3 3877 14384 27.0 2 Orchard Grass 4 5474 25990 21.1 2 A. fumigatus 11 3953 23180 17.1 23 Median 12.5 9707 22843 42.2 5.0 Juniper 3 1033 17540 5.9 1 Sweet Vernal Grass 2 1667 35263 4.7 5 Ash 3 767 17073 4.5 1 Bermuda Grass 3 903 21083 4.3 2 English Plantain 2 688 16963 4.1 1 Giant Ragweed 3 533 13960 3.8 1 American Cockroach 3 690 27854 2.5 5 Dog epithelia 5 353 14616 2.4 4 Date Palm 3 233 23417 1.0 1 Black Walnut 0 0 28913 0.0 2 Mugwort 0 0 21793 0.0 4 Western Ragweed 0 0 16733 0.0 1 Median 3 611 19312 3.1 1.5

TABLE 4 HLA restriction of prevalently recognized epitope regions Donors % Total Region restriction(s) Organism Source responding responding SFC DR DQ DP Alt. Rot Fungus Alt a 1 (6-25) 4 33.3 5572 4 0 0 Alt a 1 (116-135) 7 58.3 7450 3 3 0 Alt a 1 (141-157) 5 41.7 5768 5 0 0 Alt a 5 (51-65) 2 16.7 3450 1 0 0 Alt a 6 (161-175) 2 16.7 1238 0 0 1 Alt a 7 (6-20) 2 16.7 727 1 0 0 Alt a 7 (190-204) 2 16.7 397 0 0 0 A. fumigatus Asp f 17 (91-105) 2 18.2 813 1 0 0 C. herbarum Cla h 5 (6-20) 2 20.0 247 0 0 0 Cla h 6 (161-175) 3 30.0 2082 1 0 1 Cla h 8 (101-115) 2 20.0 317 0 0 0 Cla h 8 (151-165) 2 20.0 470 1 0 0 Cla h 8 (181-195) 2 20.0 817 0 0 0 Cla h 8 (236-250) 2 20.0 4067 1 1 0 P. chrysogenum Pen ch 13 (101-115) 2 20.0 677 1 0 0 Pen ch 13 (271-285) 2 20.0 1463 1 0 0 Pen ch 18 (226-240) 2 20.0 607 1 0 0 Pen ch 18 (291-305) 3 30.0 2480 2 0 0 Cat epithelia Fel d 1 (41-60) 3 27.3 6065 3 0 0 Fel d 1 (78-92) 3 27.3 3453 3 0 0 Fel d 1 (21-40) 6 54.5 7128 2 0 2 Fel d 1 (36-50) 2 18.2 2837 1 0 0 D. farinae Der f 1 (131-150) 2 20.0 1448 2 0 0 D. pteronyssinus Der p 1 (131-150) 2 18.2 2417 2 1 0 Der p 1 (176-190) 2 18.2 890 2 0 0 Der p 1 (226-240) 2 18.2 2783 1 1 0 Der p 4 (96-120) 3 27.3 3877 2 0 0 Der p 4 (251-265) 2 18.2 763 0 0 1 Der p 4 (321-335) 2 18.2 1570 0 1 0 Der p 4 (381-400) 4 36.4 2573 2 0 0 Der p 4 (411-430) 2 18.2 1117 0 0 1 Der p 4 (482-502) 2 18.2 1160 1 0 0 Alder Aln g 1 (11-30) 2 16.7 2036 0 1 1 Aln g 1 (111-125) 2 16.7 1244 1 1 0 Birch Bet v 1 (96-110) 2 20.0 462 0 0 0 Bet v 1 (111-125) 3 30.0 1132 2 0 0 Bet v 6 (211-230) 2 20.0 807 0 1 0 Cypress Cha o 1 (86-110) 3 30.0 1922 1 0 0 Cha o 1 (211-225) 2 20.0 835 0 0 0 Cup a 1 (71-90) 3 30.0 3067 1 0 0 Cup a 1 (316-330) 3 30.0 740 1 1 0 Cup s 1 (91-115) 3 30.0 3627 1 0 0 Cup s 1 (211-225) 2 20.0 1650 1 0 0 White Oak Que a 1 (11-35) 8 61.5 7183 1 0 3 Que a 1 (141-155) 4 30.8 1337 1 0 1 Orchard Grass Dac g 1 (181-195) 4 36.4 2795 2 0 0 Dac g 2 (11-30) 2 18.2 1917 1 0 0 Dac g 4 (1-15) 3 27.3 482 0 0 0 Rye Grass Lol p 1 (176-190) 3 27.3 1180 1 0 0 Lol p 2 (51-70) 4 36.4 1163 3 0 0 Lol p 2 (76-90) 3 27.3 1253 0 2 0 Lol p 3 (11-25) 3 27.3 2070 2 0 0 Lol p 3 (51-65) 2 18.2 1803 1 0 0 Lol p 3 (76-90) 2 18.2 3342 0 0 0 Lol p 5 (71-85) 3 27.3 3325 0 2 1 Lol p 5 (156-175) 3 27.3 3437 0 2 0 Lol p 5 (236-250) 2 18.2 460 0 0 0 Lol p 5 (306-320) 2 18.2 1695 0 1 0 Lol p 5 (181-195) 3 27.3 2260 1 1 1 Lol p 11 (116-135) 2 18.2 2393 0 0 1 Canary Grass Pha a 1 (186-200) 4 40.0 2095 3 1 0 Pha a 5 (121-140) 3 30.0 1680 1 1 0 Pha a 5 (166-180) 2 20.0 2222 0 1 1 Pha a 5 (181-195) 2 20.0 493 2 0 0 Pha a 5 (201-220) 4 40.0 1502 3 1 0 Ken. Blue Grass Poa p 1 (121-135) 3 25.0 1357 1 0 0 Poa p 5 (51-75) 7 58.3 3528 0 1 1 Poa p 5 (121-135) 3 25.0 435 1 0 0 Poa p 5 (131-145) 2 16.7 243 1 0 0 Poa p 5 (176-190) 6 50.0 1862 3 0 1 Poa p 5 (211-230) 4 33.3 1738 3 0 0 Poa p 5 (221-235) 2 16.7 1370 1 0 0 Russian Thistle Sal k 1 1 (191-205) 2 20.0 2060 0 1 1 Sal k 1 1 (321-335) 2 20.0 1947 1 0 1

TABLE 5 Complete list of peptides synthesized. (SEQ. ID. NOs.: 1-1,411, in Taxonomy Source SwissProt GI Posi- order of Organism ID protein ID number tion appearance) Len. Peptide ID Alder 3517 Aln g 1 P38948 730050 1 MGVFNYEAETPSVIP 15 3324.0008 Alder 3517 Aln g 1 P38948 730050 11 PSVIPAARLFKAFIL 15 3324.0004 Alder 3517 Aln g 1 P38948 730050 16 AARLFKAFILDGDKL 15 3324.0003 Alder 3517 Aln g 1 P38948 730050 76 DRVNFKYSFSVIEGG 15 3324.0002 Alder 3517 Aln g 1 P38948 730050 111 GGSILKISNKFHTKG 15 3324.0007 Alder 3517 Aln g 1 P38948 730050 136 IEKEKAVGLLKAVES 15 3324.0006 Alder 3517 Aln g 1 P38948 730050 141 AVGLLKAVESYLLAH 15 3324.0001 Alder 3517 Aln g 1 P38948 730050 146 KAVESYLLAHSDAYN 15 3324.0005 Alder 3517 Aln g 4 O81701 14423845 56 TDGDGFISFQEFTNF 15 3324.0010 Alder 3517 Aln g 4 O81701 14423845 61 FISFQEFTNFARANR 15 3324.0009 Alder 3517 Aln g 4 O81701 14423845 71 ARANRGLVKDVAKIF 15 3337.0001 Alternaria rot fungus 5599 Alt a 1 P79085 14423645 1 MQFTTIASLFAAAGL 15 3324.0012 Alternaria rot fungus 5599 Alt a 1 P79085 14423645 6 IASLFAAAGLAAAAP 15 3324.0015 Alternaria rot fungus 5599 Alt a 1 P79085 14423645 11 AAAGLAAAAPLESRQ 15 3324.0017 Alternaria rot fungus 5599 Alt a 1 P79085 14423645 111 KVSDDITYVATATLP 15 3324.0013 Alternaria rot fungus 5599 Alt a 1 P79085 14423645 116 ITYVATATLPNYCRA 15 3324.0014 Alternaria rot fungus 5599 Alt a 1 P79085 14423645 141 QGVADAYITLVTLPK 15 3324.0011 Alternaria rot fungus 5599 Alt a 1 P79085 14423645 143 VADAYITLVTLPKSS 15 3324.0016 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 16 QPTGLFINNEFVKAV 15 3324.0023 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 56 DVDIAVAAARKAFNG 15 3324.0032 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 81 GKLLNKLADLFEKNA 15 3324.0022 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 141 DTAPDSFNYIRKEPI 15 3324.0021 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 146 SFNYIRKEPIGVCGQ 15 3324.0033 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 161 IIPWNFPILMWSWKI 15 3324.0019 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 301 GSRIYVQEEIYDKFI 15 3324.0025 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 306 VQEEIYDKFIQRFKE 15 3324.0024 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 311 YDKFIQRFKERAAQN 15 3324.0028 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 341 VSQLQFDRIMGYIEE 15 3324.0034 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 371 GDKGYFIEPTIFSNV 15 3324.0018 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 421 YGLAAAVHTSNLTTA 15 3324.0031 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 431 NLTTAIEVANALRAG 15 3324.0027 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 436 IEVANALRAGTVWVN 15 3324.0030 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 446 TVWVNSYNTLHWQLP 15 3324.0020 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 476 AALDNYIQTKTVSIR 15 3324.0026 Alternaria rot fungus 5599 Alt a 10 P42041 76666767 481 YIQTKTVSIRLGDVL 15 3324.0029 Alternaria rot fungus 5599 Alt a 12 P49148 1350779 1 MSTSELATSYAALIL 15 3324.0036 Alternaria rot fungus 5599 Alt a 12 P49148 1350779 6 LATSYAALILADDGV 15 3324.0035 Alternaria rot fungus 5599 Alt a 12 P49148 1350779 26 KLQSLIKAAKIEEVE 15 3324.0037 Alternaria rot fungus 5599 Alt a 12 P49148 1350779 41 PIWTTLFAKALEGKD 15 3324.0039 Alternaria rot fungus 5599 Alt a 12 P49148 1350779 76 LLLRWRAADAAPAAE 15 3324.0038 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 6 SELAVQKLVLFAVKG 15 3324.0041 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 11 QKLVLFAVKGTATST 15 3324.0044 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 26 HNTVRPLILLDELGV 15 3324.0049 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 76 DTLRAWESTSTLMYI 15 3324.0048 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 111 INNWLTLHTAALGPT 15 3324.0043 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 126 AKYWLYFYKLHPEKL 15 3324.0040 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 141 PKTIEKLRSNITVQY 15 3324.0051 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 146 KLRSNITVQYDILER 15 3324.0050 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 166 GQQYLAWLNEKFKRS 15 3324.0045 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 171 AWLNEKFKRSSYNRR 15 3324.0042 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 191 LCYEKYRRVVRAGVK 15 3324.0046 Alternaria rot fungus 5599 Alt a 13 Q6R4B4 41057621 201 RAGVKVAQTARVVCP 15 3324.0047 Alternaria rot fungus 5599 Alt a 3 P78983 1850544 41 SAKNALESYAYSLRN 15 3324.0053 Alternaria rot fungus 5599 Alt a 3 P78983 1850544 46 LESYAYSLRNTLSDS 15 3324.0052 Alternaria rot fungus 5599 Alt a 3 P78983 1850544 91 ATKDEYESQQKELEG 15 3337.0002 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 26 ADKVVLVAYFAADDK 15 3324.0054 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 56 NFLFGATNDAALAKA 15 3324.0061 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 96 MRTYPRLRKVASTPL 15 3324.0060 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 101 RLRKVASTPLIGEVG 15 3324.0059 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 116 PETYAGYMAAGIPLA 15 3324.0057 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 121 GYMAAGIPLAYIFAE 15 3324.0056 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 126 GIPLAYIFAETPEER 15 3324.0058 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 156 GEINFATIDAKSFGQ 15 3324.0062 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 286 DELSKLVTIAKVDAT 15 3324.0063 Alternaria rot fungus 5599 Alt a 4 Q00002 1006624 306 DEIQGFLPSSLFPLA 15 3324.0055 Alternaria rot fungus 5599 Alt a 5 P42037 467617 1 MKHLAAYLLLGLGGN 15 3324.0064 Alternaria rot fungus 5599 Alt a 5 P42037 467617 21 ADVKAVLESVGIEAD 15 3337.0003 Alternaria rot fungus 5599 Alt a 5 P42037 467617 51 INELIASGSEKLASV 15 3324.0065 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 1 MTITKIHARSVYDSR 15 3324.0075 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 106 LGANAILGVSMAIAK 15 3324.0069 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 111 ILGVSMAIAKAAAAE 15 3324.0070 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 116 MAIAKAAAAEKGVPL 15 3324.0074 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 161 GGRLAFQEFMIVPCE 15 3324.0073 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 186 GAEVYQKLKALAKKT 15 3324.0072 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 241 IKIAMDVASSEFYKA 15 3324.0077 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 271 KSKWLTYEQLAEMYK 15 3324.0068 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 306 EAWSYFFKTYDGQIV 15 3324.0067 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 336 IELKSCNALLLKVNQ 15 3324.0066 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 341 CNALLLKVNQIGTIT 15 3324.0071 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 366 GAGWGVMVSHRSGET 15 3324.0078 Alternaria rot fungus 5599 Alt a 6 Q9HDT3 14423684 406 ERLAKLNQILRIEEE 15 3324.0076 Alternaria rot fungus 5599 Alt a 7 P42058 467619 6 AIVYYSMYGHIKKMA 15 3324.0085 Alternaria rot fungus 5599 Alt a 7 P42058 467619 31 GGDAKLFQVAETLPQ 15 3324.0082 Alternaria rot fungus 5599 Alt a 7 P42058 467619 36 LFQVAETLPQEVLDK 15 3324.0084 Alternaria rot fungus 5599 Alt a 7 P42058 467619 66 PAVLEEFDGILFGIP 15 3324.0087 Alternaria rot fungus 5599 Alt a 7 P42058 467619 106 FWGKYAGVFVSTGTL 15 3324.0086 Alternaria rot fungus 5599 Alt a 7 P42058 467619 141 IYVPLGYKTAFSMLA 15 3324.0079 Alternaria rot fungus 5599 Alt a 7 P42058 467619 146 GYKTAFSMLANLDEV 15 3324.0081 Alternaria rot fungus 5599 Alt a 7 P42058 467619 181 SELELNIAQAQGKAF 15 3324.0083 Alternaria rot fungus 5599 Alt a 7 P42058 467619 190 AQGKAFYEAVAKAHQ 15 3324.0080 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 6 PQATELKDLFSLKGK 15 3324.0090 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 11 LKDLFSLKGKVVIVT 15 3324.0091 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 46 ADLAITYNSRAEGAE 15 3324.0094 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 151 TGSLVITSSMSGHIA 15 3324.0093 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 176 VAKAGCIHLAKSLAN 15 3324.0096 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 181 CIHLAKSLANEWRDF 15 3324.0095 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 216 QDIQKLWHSMIPMGR 15 3324.0092 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 236 ELKGAYVYFASDASS 15 3324.0088 Alternaria rot fungus 5599 Alt a 8 P0C0Y4 23344707 241 YVYFASDASSYCTGS 15 3324.0089 American cockroach 6978 Per a 1 O18527 2231297 1 INEIHSIIGLPPFVP 15 3324.1371 American cockroach 6978 Per a 1 O18527 2231297 41 DELKALFQEKLETSP 15 3324.1356 American cockroach 6978 Per a 1 O18527 2231297 51 LETSPDFKALYDAIR 15 3324.1378 American cockroach 6978 Per a 1 O18527 2231297 56 DFKALYDAIRSPEFQ 15 3324.1375 American cockroach 6978 Per a 1 O18527 2231297 66 SPEFQSIISTLNAMQ 15 3324.1359 American cockroach 6978 Per a 1 O18527 2231297 71 SIISTLNAMQRSEHH 15 3324.1357 American cockroach 6978 Per a 1 O18527 2231297 91 KGVDVDHFIQLIRAL 15 3324.1363 American cockroach 6978 Per a 1 O18527 2231297 96 DHFIQLIRALFGLSR 15 3324.1347 American cockroach 6978 Per a 1 O18527 2231297 101 LIRALFGLSRAARNL 15 3324.1364 American cockroach 6978 Per a 1 O18527 2231297 106 FGLSRAARNLQDDLN 15 3324.1369 American cockroach 6978 Per a 1 O18527 2231297 116 QDDLNDFLHSLEPIS 15 3324.1373 American cockroach 6978 Per a 1 O18527 2231297 151 LHADDFHKIITTIEA 15 3324.1379 American cockroach 6978 Per a 1 O18527 2231297 156 FHKIITTIEALPEFA 15 3324.1351 American cockroach 6978 Per a 1 O18527 2231297 166 LPEFANFYNFLKEHG 15 3324.1355 American cockroach 6978 Per a 1 O18527 2231297 171 NFYNFLKEHGLDVVD 15 3324.1372 American cockroach 6978 Per a 1 O18527 2231297 181 LDVVDYINEIHSIIG 15 3324.1361 American cockroach 6978 Per a 1 O18527 2231297 186 YINEIHSIIGLPPFV 15 3324.1365 American cockroach 6978 Per a 1 O18527 2231297 211 VGINGLIDDVIAILP 15 3324.1368 American cockroach 6978 Per a 1 O18527 2231297 216 LIDDVIAILPVDELK 15 3324.1362 American cockroach 6978 Per a 1 O18527 2231297 221 IAILPVDELKALFQE 15 3324.1370 American cockroach 6978 Per a 1 O18527 2231297 226 VDELKALFQEKLETS 15 3324.1360 American cockroach 6978 Per a 1 O18527 2231297 241 PDFKALYDAIRSPEF 15 3324.1380 American cockroach 6978 Per a 1 O18527 2231297 251 RSPEFQSIISTLNAM 15 3324.1381 American cockroach 6978 Per a 1 O18527 2231297 306 DLNDFLALIPTDQIL 15 3324.1348 American cockroach 6978 Per a 1 O18527 2231297 311 LALIPTDQILAIAMD 15 3324.1377 American cockroach 6978 Per a 1 O18527 2231297 316 TDQILAIAMDYLAND 15 3324.1354 American cockroach 6978 Per a 1 O18527 2231297 321 AIAMDYLANDAEVQE 15 3324.1374 American cockroach 6978 Per a 1 O18527 2231297 331 AEVQELVAYLQSDDF 15 3324.1350 American cockroach 6978 Per a 1 O18527 2231297 341 QSDDFHKIITTIEAL 15 3324.1353 American cockroach 6978 Per a 1 O18527 2231297 346 HKIITTIEALPEFAN 15 3324.1352 American cockroach 6978 Per a 1 O18527 2231297 356 PEFANFYNFLKEHGL 15 3324.1349 American cockroach 6978 Per a 1 O18527 2231297 361 FYNFLKEHGLDVVDY 15 3324.1376 American cockroach 6978 Per a 1 O18527 2231297 371 DVVDYINEIHSIIGL 15 3324.1358 American cockroach 6978 Per a 1 O18527 2231297 431 DFKALYDAIDLRSSR 15 3324.1366 American cockroach 6978 Per a 1 O18527 2231297 432 FKALYDAIDLRSSRA 15 3324.1367 American cockroach 6978 Per a 10 Q1M0X9 60678799 1 MLRYLVLASLIACSL 15 3324.1382 American cockroach 6978 Per a 10 Q1M0X9 60678799 6 VLASLIACSLSAVPK 15 3324.1386 American cockroach 6978 Per a 10 Q1M0X9 60678799 41 YPYQLSFEYYGSHMC 15 3324.1383 American cockroach 6978 Per a 10 Q1M0X9 60678799 96 HQATQLIANPNYDYY 15 3324.1393 American cockroach 6978 Per a 10 Q1M0X9 60678799 106 NYDYYTIDFDVAVAR 15 3324.1388 American cockroach 6978 Per a 10 Q1M0X9 60678799 111 TIDFDVAVARVSPAF 15 3324.1385 American cockroach 6978 Per a 10 Q1M0X9 60678799 116 VAVARVSPAFSYGTG 15 3324.1389 American cockroach 6978 Per a 10 Q1M0X9 60678799 161 TLPSQLQVVSVPIVS 15 3324.1387 American cockroach 6978 Per a 10 Q1M0X9 60678799 166 LQVVSVPIVSRSECN 15 3324.1390 American cockroach 6978 Per a 10 Q1M0X9 60678799 236 PGVYSNVASLKGFIT 15 3324.1392 American cockroach 6978 Per a 10 Q1M0X9 60678799 241 NVASLKGFITEQTGV 15 3324.1391 American cockroach 6978 Per a 10 Q1M0X9 60678799 242 VASLKGFITEQTGVN 15 3324.1384 American cockroach 6978 Per a 3 Q25640 1580794 1 LNAFNMYFRYIYPTW 15 3324.1401 American cockroach 6978 Per a 3 Q25640 1580794 6 MYFRYIYPTWFNTTL 15 3324.1397 American cockroach 6978 Per a 3 Q25640 1580794 31 QFYYTYHQIYARYFL 15 3324.1399 American cockroach 6978 Per a 3 Q25640 1580794 36 YHQIYARYFLERLSN 15 3324.1398 American cockroach 6978 Per a 3 Q25640 1580794 41 ARYFLERLSNSLPDV 15 3324.1395 American cockroach 6978 Per a 3 Q25640 1580794 56 KPFQYSKPLKTGYNP 15 3324.1409 American cockroach 6978 Per a 3 Q25640 1580794 91 NIDLFYVSDIKNYES 15 3324.1411 American cockroach 6978 Per a 3 Q25640 1580794 141 GTSNSPYQYFYGSIF 15 3324.1408 American cockroach 6978 Per a 3 Q25640 1580794 146 PYQYFYGSIFHFYRL 15 3324.1402 American cockroach 6978 Per a 3 Q25640 1580794 151 YGSIFHFYRLLVGHV 15 3324.1394 American cockroach 6978 Per a 3 Q25640 1580794 156 HFYRLLVGHVVDPYH 15 3324.1412 American cockroach 6978 Per a 3 Q25640 1580794 191 FYQLWKRIDHIVQKY 15 3324.1404 American cockroach 6978 Per a 3 Q25640 1580794 196 KRIDHIVQKYKNRLP 15 3324.1410 American cockroach 6978 Per a 3 Q25640 1580794 231 KLYTYFEHFEHSLGN 15 3324.1405 American cockroach 6978 Per a 3 Q25640 1580794 236 FEHFEHSLGNAMYLG 15 3324.1413 American cockroach 6978 Per a 3 Q25640 1580794 271 PFTYNIEVSSDKAQD 15 3324.1414 American cockroach 6978 Per a 3 Q25640 1580794 281 DKAQDVYVRIFLGPK 15 3324.1407 American cockroach 6978 Per a 3 Q25640 1580794 346 YRNLFKKVSDALEGK 15 3324.1403 American cockroach 6978 Per a 3 Q25640 1580794 386 GGQTFTFYVIVTPYV 15 3324.1396 American cockroach 6978 Per a 3 Q25640 1580794 391 TFYVIVTPYVKQDEH 15 3324.1400 American cockroach 6978 Per a 3 Q25640 1580794 446 YTPNMYFKDVVIFHK 15 3324.1415 American cockroach 6978 Per a 3 Q25640 1580794 451 YFKDVVIFHKKYDEV 15 3324.1406 American cockroach 6978 Per a 6 Q1M0Y3 60678791 6 DEQIQLLKKAFDAFD 15 3324.1420 American cockroach 6978 Per a 6 Q1M0Y3 60678791 31 MVGTILEMLGHPLDD 15 3324.1418 American cockroach 6978 Per a 6 Q1M0Y3 60678791 61 GELEFQEFVTLAARF 15 3324.1417 American cockroach 6978 Per a 6 Q1M0Y3 60678791 66 QEFVTLAARFLVEED 15 3324.1416 American cockroach 6978 Per a 6 Q1M0Y3 60678791 101 GYITTTVLREILKEL 15 3324.1419 American cockroach 6978 Per a 6 Q1M0Y3 60678791 106 TVLREILKELDDKLT 15 3324.1421 American cockroach 6978 Per a 7 Q9UB83 4468639 101 RSEERLATATAKLAE 15 3324.1423 American cockroach 6978 Per a 7 Q9UB83 4468639 191 VELEEELRVVGNNLK 15 3324.1425 American cockroach 6978 Per a 7 Q9UB83 4468639 216 LREEEYKQQIKTLTT 15 3324.1424 American cockroach 6978 Per a 7 Q9UB83 4468639 221 YKQQIKTLTTRLKEA 15 3324.1422 Ash 38873 Fra e 1 Q6U740 34978692 21 DTCRARFITKLSEFI 15 3324.1102 Ash 38873 Fra e 1 Q6U740 34978692 26 RFITKLSEFITGASV 15 3324.1101 Ash 38873 Fra e 1 Q6U740 34978692 31 LSEFITGASVRLQCR 15 3324.1105 Ash 38873 Fra e 1 Q6U740 34978692 76 NEFCEITLLSSGRKD 15 3324.1106 Ash 38873 Fra e 1 Q6U740 34978692 101 KPSLKFILNTVNGTT 15 3324.1103 Ash 38873 Fra e 1 Q6U740 34978692 121 LGFFKKEALPQCAQV 15 3324.1104 Aspergillus fumigatus 330879 Asp f 1 P67875 66845737 1 MVAIKNLFLLAATAV 15 3324.0121 Aspergillus fumigatus 330879 Asp f 1 P67875 66845737 6 NLFLLAATAVSVLAA 15 3324.0122 Aspergillus fumigatus 330879 Asp f 1 P67875 66845737 46 DKRLLYSQAKAESNS 15 3324.0123 Aspergillus fumigatus 330879 Asp f 1 P67875 66845737 116 DDHYLLEFPTFPDGH 15 3337.0014 Aspergillus fumigatus 330879 Asp f 1 P67875 66845737 146 ARVIYTYPNKVFCGI 15 3324.0124 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 1 MVVFSKVTAVVVGLS 15 3324.0128 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 6 KVTAVVVGLSTIVSA 15 3324.0129 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 11 VVGLSTIVSAVPVVQ 15 3324.0126 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 16 TIVSAVPVVQPRKGF 15 3324.0134 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 26 PRKGFTINQVARPVT 15 3324.0136 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 41 NKKTVNLPAVYANAL 15 3324.0132 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 46 NLPAVYANALTKYGG 15 3324.0131 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 61 TVPDSVKAAASSGSA 15 3324.0137 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 106 ADLWVFSSELSASQS 15 3324.0127 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 171 VEAASHISSQFVQDK 15 3324.0125 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 186 DNDGLLGLAFSSINT 15 3324.0133 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 191 LGLAFSSINTVSPRP 15 3324.0135 Aspergillus fumigatus 330879 Asp f 10 Q12547(IUIS) 85541049 221 LFAVTLKYHAPGTYD 15 3324.0130 Aspergillus fumigatus 746128 Asp f 11 Q9Y7F6 5019414 11 VFFDVEYAPVGTAET 15 3324.0143 Aspergillus fumigatus 746128 Asp f 11 Q9Y7F6 5019414 26 KVGRIVFNLFDKDVP 15 3324.0144 Aspergillus fumigatus 746128 Asp f 11 Q9Y7F6 5019414 61 STFHRIIPNFMIQGG 15 3324.0140 Aspergillus fumigatus 746128 Asp f 11 Q9Y7F6 5019414 101 KHDKKGILSMANAGP 15 3324.0141 Aspergillus fumigatus 746128 Asp f 11 Q9Y7F6 5019414 116 NTNGSQFFITTAVTS 15 3324.0138 Aspergillus fumigatus 746128 Asp f 11 Q9Y7F6 5019414 121 QFFITTAVTSWLDGK 15 3324.0139 Aspergillus fumigatus 746128 Asp f 11 Q9Y7F6 5019414 146 KSYSVVKEIEALGSS 15 3324.0142 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 1 MSSETFEFQAEISQL 15 3324.0177 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 6 FEFQAEISQLLSLII 15 3324.0146 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 11 EISQLLSLIINTVYS 15 3324.0151 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 16 LSLIINTVYSNKEIF 15 3324.0165 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 26 NKEIFLRELISNASD 15 3324.0147 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 31 LRELISNASDALDKI 15 3324.0156 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 41 ALDKIRYQSLSDPTK 15 3324.0172 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 96 IARSGTKQFMEALSA 15 3324.0173 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 101 TKQFMEALSAGADIS 15 3324.0180 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 116 MIGQFGVGFYSAYLV 15 3324.0166 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 121 GVGFYSAYLVADRVT 15 3324.0149 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 126 SAYLVADRVTVVSKN 15 3324.0171 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 171 TKIILHLKDEQTDYL 15 3324.0179 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 191 KEVVRKHSEFISYPI 15 3324.0161 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 196 KHSEFISYPIYLHVL 15 3324.0155 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 281 ITQEEYASFYKSLSN 15 3324.0152 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 301 LAVKHFSVEGQLEFR 15 3324.0170 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 311 QLEFRAILYVPKRAP 15 3324.0150 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 336 NNIKLYVRRVFITDD 15 3324.0157 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 341 YVRRVFITDDATDLI 15 3324.0158 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 391 KNIVKKTLELFNEIA 15 3324.0175 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 406 EDREQFDKFYSAFSK 15 3324.0159 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 411 FDKFYSAFSKNIKLG 15 3324.0154 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 436 LAKLLRYQSTKSGDE 15 3324.0176 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 466 KQIYYITGESIKAVA 15 3324.0164 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 476 IKAVAKSPFLDSLKQ 15 3324.0174 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 491 KNFEVLFLVDPIDEY 15 3324.0163 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 541 EKEYENLAKSLKNIL 15 3324.0160 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 546 NLAKSLKNILGDKVE 15 3324.0178 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 581 GWSANMERIMKAQAL 15 3324.0167 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 586 MERIMKAQALRDTSM 15 3324.0168 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 631 ENDRTVKSITQLLFE 15 3324.0153 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 636 VKSITQLLFETSLLV 15 3324.0145 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 641 QLLFETSLLVSGFTI 15 3324.0148 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 661 FAERIHKLVSLGLNI 15 3324.0162 Aspergillus fumigatus 330879 Asp f 12 P40292 1930153 666 HKLVSLGLNIDEEAE 15 3324.0169 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 1 MLSIKRTLLLLGAVL 15 3324.0183 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 6 RTLLLLGAVLPAVFG 15 3324.0181 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 81 KSYKIKDFAAYAGSF 15 3324.0189 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 111 HVEEDQIWYLDALTT 15 3324.0182 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 116 QIWYLDALTTQKGAP 15 3324.0186 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 141 QASTDYIYDTSAGAG 15 3324.0187 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 146 YIYDTSAGAGTYAYV 15 3324.0195 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 211 KTNLLSVKVFQGESS 15 3324.0185 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 216 SVKVFQGESSSTSII 15 3324.0193 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 231 LDGFNWAVNDIVSKG 15 3324.0192 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 256 LGGGYSYAFNNAVEN 15 3324.0190 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 296 SAPNALTVAAINKSN 15 3324.0194 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 351 ATPHIVGLSVYLMGL 15 3324.0184 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 356 VGLSVYLMGLENLSG 15 3324.0188 Aspergillus fumigatus 330879 Asp f 13 P28296 83305202 361 YLMGLENLSGPAAVT 15 3324.0191 Aspergillus fumigatus 330879 Asp f 15 O60022 66853233 1 MKFTTPISLISLFVS 15 3324.0197 Aspergillus fumigatus 330879 Asp f 15 O60022 66853233 6 PISLISLFVSSALAA 15 3324.0196 Aspergillus fumigatus 330879 Asp f 15 O60022 66853233 11 SLFVSSALAAPTPEN 15 3324.0198 Aspergillus fumigatus 330879 Asp f 15 O60022 66853233 91 GKCYKLQYEQNTIYV 15 3324.0202 Aspergillus fumigatus 330879 Asp f 15 O60022 66853233 96 LQYEQNTIYVTAIDA 15 3324.0201 Aspergillus fumigatus 330879 Asp f 15 O60022 66853233 101 NTIYVTAIDAAPGGF 15 3324.0200 Aspergillus fumigatus 330879 Asp f 15 O60022 66853233 111 APGGFNIATSAMDQL 15 3324.0199 Aspergillus fumigatus 746128 Asp f 16 O74682(IUIS) 3643812 1 MYFKYTAAALAAVLP 15 3324.0204 Aspergillus fumigatus 746128 Asp f 16 O74682(IUIS) 3643812 6 TAAALAAVLPLCSAQ 15 3324.0205 Aspergillus fumigatus 746128 Asp f 16 O74682(IUIS) 3643812 81 PTIDTDFYFFFGKAE 15 3324.0206 Aspergillus fumigatus 746128 Asp f 16 O74682(IUIS) 3643812 86 DFYFFFGKAEVVMKA 15 3324.0203 Aspergillus fumigatus 746128 Asp f 16 O74682(IUIS) 3643812 196 PQTPMRLRLAAGPAA 15 3324.0207 Aspergillus fumigatus 746128 Asp f 17 O60025 2980819 11 VGVISDISAQTSALA 15 3324.0208 Aspergillus fumigatus 746128 Asp f 17 O60025 2980819 91 SKKDKFVAANAGGTV 15 3324.0212 Aspergillus fumigatus 746128 Asp f 17 O60025 2980819 101 AGGTVYEDLKAQYTA 15 3324.0213 Aspergillus fumigatus 746128 Asp f 17 O60025 2980819 111 AQYTAADSLAKAISA 15 3324.0214 Aspergillus fumigatus 746128 Asp f 17 O60025 2980819 116 ADSLAKAISAKVPES 15 3324.0209 Aspergillus fumigatus 746128 Asp f 17 O60025 2980819 131 LSDIAAQLSAGITAA 15 3324.0211 Aspergillus fumigatus 746128 Asp f 17 O60025 2980819 136 AQLSAGITAAIQKGI 15 3324.0210 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 1 MKGYLSLSILPLLVA 15 3324.0217 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 6 SLSILPLLVAASPVV 15 3324.0218 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 11 PLLVAASPVVVDSIH 15 3324.0221 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 26 NGAAPILSSMNAKEV 15 3324.0231 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 46 VVFKKHVNAESAAAH 15 3324.0224 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 61 HSWVQDIHSAQNERV 15 3324.0220 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 91 LGLKNTFDIAGSLVG 15 3324.0227 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 96 TFDIAGSLVGYSGHF 15 3324.0233 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 236 YAVKVLRSSGSGTMS 15 3324.0225 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 276 KGFKGSVANMSLGGG 15 3324.0229 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 291 KSRTLEAAVNAGVEA 15 3324.0230 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 301 AGVEAGLHFAVAAGN 15 3324.0222 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 381 MASPHIAGLLAYFVS 15 3324.0219 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 386 IAGLLAYFVSLQPSK 15 3324.0215 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 411 PKKLKKDIIAIATQG 15 3324.0232 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 416 KDIIAIATQGALTDI 15 3324.0216 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 451 IIASGGYKVNASVKD 15 3324.0226 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 476 KLLTEELGAIYSEIH 15 3324.0223 Aspergillus fumigatus 330879 Asp f 18 P87184 66851354 481 ELGAIYSEIHDAAVA 15 3324.0228 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 1 MAALLRLAVLLPLAA 15 3324.0236 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 6 RLAVLLPLAAPLVAT 15 3324.0235 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 16 PLVATLPTSPVPIAA 15 3324.0242 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 36 EPVFFSWDAGAVTSF 15 3324.0238 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 41 SWDAGAVTSFPIHSS 15 3324.0239 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 156 TTRRWLVSMCSQGYT 15 3324.0243 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 206 YDEVIALAKSNGTES 15 3324.0241 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 221 THDSEALQYFALEAY 15 3324.0240 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 226 ALQYFALEAYAFDIA 15 3324.0234 Aspergillus fumigatus 330879 Asp f 2 P79017 66849502 231 ALEAYAFDIAAPGVG 15 3324.0237 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 1 MPISKIHARSVYDSR 15 3324.0256 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 106 LGANAILGVSLAVAK 15 3324.0245 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 111 ILGVSLAVAKAGAAE 15 3324.0248 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 161 GGRLAFQEFMIVPDS 15 3324.0252 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 166 FQEFMIVPDSAPSFS 15 3324.0251 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 186 GAEVYQKLKALAKKK 15 3324.0254 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 241 IKIAMDVASSEFYKA 15 3324.0255 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 271 PSKWLTYEQLADLYK 15 3324.0247 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 281 ADLYKSLAAKYPIVS 15 3324.0244 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 306 EAWSYFYKTSDFQIV 15 3324.0250 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 336 IELKSCNALLLKVNQ 15 3324.0246 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 341 CNALLLKVNQIGTLT 15 3324.0249 Aspergillus fumigatus 330879 Asp f 22 Q96X30 13925873 406 ERLAKLNQILRIEEE 15 3324.0253 Aspergillus fumigatus 330879 Asp f 23 Q8NKF4 66853155 71 EIVEAVTIIETPPLV 15 3324.0259 Aspergillus fumigatus 330879 Asp f 23 Q8NKF4 66853155 96 PRGLRSLTTVWAEHL 15 3324.0257 Aspergillus fumigatus 330879 Asp f 23 Q8NKF4 66853155 151 RIKKYCTVVRVLAHT 15 3324.0260 Aspergillus fumigatus 330879 Asp f 23 Q8NKF4 66853155 156 CTVVRVLAHTQIRKT 15 3324.0258 Aspergillus fumigatus 330879 Asp f 27 Q4WWX5 71000343 1 MVVKTFFDITIDGQP 15 3324.0265 Aspergillus fumigatus 330879 Asp f 27 Q4WWX5 71000343 21 FKLFDEVVPKTVENF 15 3324.0266 Aspergillus fumigatus 330879 Asp f 27 Q4WWX5 71000343 46 GYKGSSFHRIIPQFM 15 3324.0261 Aspergillus fumigatus 330879 Asp f 27 Q4WWX5 71000343 51 SFHRIIPQFMLQGGD 15 3324.0263 Aspergillus fumigatus 330879 Asp f 27 Q4WWX5 71000343 91 HDKPGLLSMANAGKN 15 3324.0267 Aspergillus fumigatus 330879 Asp f 27 Q4WWX5 71000343 106 TNGSQFFITTVVTSW 15 3324.0262 Aspergillus fumigatus 330879 Asp f 27 Q4WWX5 71000343 111 FFITTVVTSWLDGAH 15 3324.0264 Aspergillus fumigatus 746128 Asp f 28 Q1RQJ1 91680607 11 NPIIYKALTSSGPVV 15 3324.0270 Aspergillus fumigatus 746128 Asp f 28 Q1RQJ1 91680607 46 LSEKYSNVRFIQVDV 15 3324.0271 Aspergillus fumigatus 746128 Asp f 28 Q1RQJ1 91680607 66 VAHEMNIRAMPTFVL 15 3324.0268 Aspergillus fumigatus 746128 Asp f 28 Q1RQJ1 91680607 71 NIRAMPTFVLYKDGQ 15 3324.0269 Aspergillus fumigatus 330879 Asp f 29 Q4WV97 70997545 41 SPVFQRLSTSEEFKN 15 3324.0273 Aspergillus fumigatus 330879 Asp f 29 Q4WV97 70997545 56 AKFYEIDVDELSEVA 15 3324.0272 Aspergillus fumigatus 330879 Asp f 29 Q4WV97 70997545 96 ANPPALEAAIKAHVA 15 3337.0015 Aspergillus fumigatus 330879 Asp f 3 O43099 2769700 46 KKVILFALPGAFTPV 15 3324.0274 Aspergillus fumigatus 330879 Asp f 3 O43099 2769700 81 VDVVAVLAYNDAYVM 15 3324.0275 Aspergillus fumigatus 330879 Asp f 3 O43099 2769700 86 VLAYNDAYVMSAWGK 15 3324.0277 Aspergillus fumigatus 330879 Asp f 3 O43099 2769700 106 GDDILFLSDPDARFS 15 3324.0279 Aspergillus fumigatus 330879 Asp f 3 O43099 2769700 141 HGKITYAALEPAKNH 15 3324.0278 Aspergillus fumigatus 330879 Asp f 3 O43099 2769700 154 NHLEFSSAETVLKHL 15 3324.0276 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 1 MQIKSFVLAASAAAT 15 3324.0280 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 6 FVLAASAAATASAAA 15 3324.0281 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 26 NKYFGIVAIHSGSAV 15 3324.0283 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 31 IVAIHSGSAVQYQPF 15 3324.0282 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 36 SGSAVQYQPFSAAKS 15 3324.0287 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 41 QYQPFSAAKSSIFAG 15 3324.0286 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 71 ATFYIQDGSLYLYAA 15 3324.0285 Aspergillus fumigatus 746128 Asp f 34 A4FSH5 133920236 76 QDGSLYLYAASATPQ 15 3324.0284 Aspergillus fumigatus 330879 Asp f 4 O60024 70989331 1 MQLKNSMLLLTALAA 15 3324.0289 Aspergillus fumigatus 330879 Asp f 4 O60024 70989331 6 SMLLLTALAAGSSVA 15 3324.0290 Aspergillus fumigatus 330879 Asp f 4 O60024 70989331 41 DTVYATINGVLVSWI 15 3324.0288 Aspergillus fumigatus 330879 Asp f 4 O60024 70989331 186 TGWYGNSALTLHLEA 15 3324.0291 Aspergillus fumigatus 330879 Asp f 4 O60024 70989331 276 GELCSIISHGLSKVI 15 3324.0293 Aspergillus fumigatus 330879 Asp f 4 O60024 70989331 286 LSKVIDAYTADLAGV 15 3324.0292 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 1 MRGLLLAGALALPAS 15 3324.0297 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 6 LAGALALPASVFAHP 15 3324.0311 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 31 TVDLNAFRLKSLAKY 15 3324.0299 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 36 AFRLKSLAKYVNATE 15 3324.0300 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 41 SLAKYVNATETVIEA 15 3324.0313 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 61 PFKPQSYVEVATQHV 15 3324.0317 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 121 GKDGKVFSYGNSFYT 15 3324.0306 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 181 ESYVFKGVSGTVSDP 15 3324.0314 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 196 KAKLVYFVKDDGTLA 15 3324.0308 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 221 SNWLLTYIDAKSGEE 15 3324.0298 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 306 SYLNNYRPSSSSLSF 15 3324.0318 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 316 SSLSFKYPYSVSSSP 15 3324.0309 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 331 PSSYIDASIIQLFYT 15 3324.0294 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 336 DASIIQLFYTANIYH 15 3324.0296 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 341 QLFYTANIYHDLLYT 15 3324.0304 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 346 ANIYHDLLYTLGFTE 15 3324.0310 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 376 GNDYVILNAQDGSGT 15 3324.0315 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 426 IVIHEYTHGLSNRLT 15 3324.0307 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 461 WSDFMATAIRLKPGD 15 3324.0319 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 491 AGGIRQYPYSTSLST 15 3324.0302 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 506 NPLTYTSVNSLNAVH 15 3324.0316 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 516 LNAVHAIGTVWASML 15 3324.0312 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 521 AIGTVWASMLYEVLW 15 3324.0305 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 526 WASMLYEVLWNLIDK 15 3324.0295 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 556 PTDGKYLAMKLVMDG 15 3324.0303 Aspergillus fumigatus 330879 Asp f 5 P46075 3776613 576 CNPNFVQARDAILDA 15 3324.0301 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 11 PYPYDALQPYISQQI 15 3324.0322 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 16 ALQPYISQQIMELHH 15 3324.0321 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 31 KKHHQTYVNGLNAAL 15 3324.0326 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 36 TYVNGLNAALEAQKK 15 3324.0323 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 56 DVPKLVSVQQAIKFN 15 3324.0325 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 61 VSVQQAIKFNGGGHI 15 3324.0328 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 111 FDKFKDAFNTTLLGI 15 3324.0324 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 166 EHAYYLQYLNDKASY 15 3324.0320 Aspergillus fumigatus 330879 Asp f 6 Q92450 18158811 171 LQYLNDKASYAKGIW 15 3324.0327 Aspergillus fumigatus 330879 Asp f 7 O42799 2879888 1 MAPIFKSLALVSALF 15 3324.0332 Aspergillus fumigatus 330879 Asp f 7 O42799 2879888 6 KSLALVSALFAAISS 15 3324.0330 Aspergillus fumigatus 330879 Asp f 7 O42799 2879888 11 VSALFAAISSAAPVN 15 3324.0331 Aspergillus fumigatus 330879 Asp f 7 O42799 2879888 96 TQPSVATFIPVAAAA 15 3324.0334 Aspergillus fumigatus 330879 Asp f 7 O42799 2879888 101 ATFIPVAAAAAAADS 15 3324.0333 Aspergillus fumigatus 330879 Asp f 7 O42799 2879888 176 LTYYDTATSASAPSS 15 3324.0335 Aspergillus fumigatus 330879 Asp f 7 O42799 2879888 196 DGFSENVVALPVGIM 15 3324.0329 Aspergillus fumigatus 330879 Asp f 8 Q9UUZ6 9887212 1 MKHLAAYLLLALAGN 15 3324.0336 Aspergillus fumigatus 330879 Asp f 8 Q9UUZ6 9887212 6 AYLLLALAGNTSPSS 15 3324.0337 Aspergillus fumigatus 330879 Asp f 8 Q9UUZ6 9887212 21 EDVKAVLSSVGIDAD 15 3324.0338 Aspergillus fumigatus 330879 Asp f 8 Q9UUZ6 9887212 36 EERLNKLIAELEGKD 15 3337.0016 Aspergillus fumigatus 330879 Asp f 8 Q9UUZ6 9887212 51 LQELIAEGSTKLASV 15 3324.0339 Aspergillus fumigatus 330879 Asp f 9 O42800(IUIS) 85540942 1 KRSFILRSADMYFKY 15 3324.0342 Aspergillus fumigatus 330879 Asp f 9 O42800(IUIS) 85540942 6 LRSADMYFKYTAAAL 15 3324.0343 Aspergillus fumigatus 330879 Asp f 9 O42800(IUIS) 85540942 11 MYFKYTAAALAAVLP 15 3324.0341 Aspergillus fumigatus 330879 Asp f 9 O42800(IUIS) 85540942 16 TAAALAAVLPLCSAQ 15 3324.0344 Aspergillus fumigatus 330879 Asp f 9 O42800(IUIS) 85540942 51 STYTADFTSASALDQ 15 3324.0346 Aspergillus fumigatus 330879 Asp f 9 O42800(IUIS) 85540942 91 PTIDTDFYFFFGKAE 15 3324.0345 Aspergillus fumigatus 330879 Asp f 9 O42800(IUIS) 85540942 96 DFYFFFGKAEVVMKA 15 3324.0340 Bermuda grass 28909 Cyn d 1 Q947S4 16076697 1 MLAVVAVVLASMVGG 15 3324.0633 Bermuda grass 28909 Cyn d 1 Q947S4 16076697 111 ITDKNYEHIAAYHFD 15 3324.0637 Bermuda grass 28909 Cyn d 1 Q947S4 16076697 116 YEHIAAYHFDLSGKA 15 3324.0634 Bermuda grass 28909 Cyn d 1 Q947S4 16076697 141 DKLRKAGELMLQFRR 15 3324.0635 Bermuda grass 28909 Cyn d 1 Q947S4 16076697 176 PNYLALLVKYAAGDG 15 3324.0636 Bermuda grass 28909 Cyn d 12 O04725 2154728 31 TVWAQSAAFPAFKPE 15 3324.0639 Bermuda grass 28909 Cyn d 12 O04725 2154728 66 FLGPTKYMVIQGEPG 15 3324.0640 Bermuda grass 28909 Cyn d 12 O04725 2154728 71 KYMVIQGEPGAVIRG 15 3324.0638 Bermuda grass 28909 Cyn d 15 Q7XYF2 32344781 1 MATLTFPVLLATMVG 15 3324.0641 Bermuda grass 28909 Cyn d 15 Q7XYF2 32344781 6 FPVLLATMVGHAWCV 15 3324.0642 Bermuda grass 28909 Cyn d 15 Q7XYF2 32344781 21 NIIFHVEESSPKFAL 15 3324.0643 Bermuda grass 28909 Cyn d 15 Q7XYF2 32344781 71 KPLKGPLNIRLRAEG 15 3337.0021 Bermuda grass 28909 Cyn d 23 Q7XYF3 32344779 1 MAKVIAIILVATMVT 15 3324.0644 Bermuda grass 28909 Cyn d 23 Q7XYF3 32344779 6 AIILVATMVTAALVP 15 3324.0645 Bermuda grass 28909 Cyn d 23 Q7XYF3 32344779 11 ATMVTAALVPIECAT 15 3324.0648 Bermuda grass 28909 Cyn d 23 Q7XYF3 32344779 56 DAAVNLMAMSFICIG 15 3324.0646 Bermuda grass 28909 Cyn d 23 Q7XYF3 32344779 61 LMAMSFICIGWAKKA 15 3324.0647 Bermuda grass 28909 Cyn d 23 Q7XYF3 32344779 91 AADQVLAAAPAHKYK 15 3324.0649 Bermuda grass 28909 Cyn d 7 P94092 14423848 26 ELTDALRTLGSTSAD 15 3324.0651 Bermuda grass 28909 Cyn d 7 P94092 14423848 31 LRTLGSTSADEVQRM 15 3324.0652 Bermuda grass 28909 Cyn d 7 P94092 14423848 56 FIDFDEFISFCNANP 15 3324.0650 Birch 3505 Bet v 1 O23754 2414158 6 YETETTSVIPAARLF 15 3324.0351 Birch 3505 Bet v 1 O23754 2414158 11 TSVIPAARLFKAFFL 15 3324.0349 Birch 3505 Bet v 1 O23754 2414158 76 DHTNFKYSYSVIEGG 15 3324.0350 Birch 3505 Bet v 1 O23754 2414158 96 LEKISNEIKIVATPD 15 3324.0353 Birch 3505 Bet v 1 O23754 2414158 111 GGSILKISNKYHTKG 15 3324.0352 Birch 3505 Bet v 1 O23754 2414158 141 GETLLRAVESYLLAH 15 3324.0347 Birch 3505 Bet v 1 O23754 2414158 146 RAVESYLLAHSDAYN 15 3324.0348 Birch 3505 Bet v 2 P25816 157830684 31 DGSVWAQSSSFPQFK 15 3324.0355 Birch 3505 Bet v 2 P25816 157830684 66 GLHLGGIKYMVIQGE 15 3324.0356 Birch 3505 Bet v 2 P25816 157830684 71 GIKYMVIQGEAGAVI 15 3324.0354 Birch 3505 Bet v 3 P43187 257675827 26 NSSFRLRSESLNTLR 15 3324.0358 Birch 3505 Bet v 3 P43187 257675827 31 LRSESLNTLRLRRIF 15 3324.0360 Birch 3505 Bet v 3 P43187 257675827 36 LNTLRLRRIFDLFDK 15 3324.0361 Birch 3505 Bet v 3 P43187 257675827 56 ITVDELSRALNLLGL 15 3324.0359 Birch 3505 Bet v 3 P43187 257675827 61 LSRALNLLGLETDLS 15 3324.0364 Birch 3505 Bet v 3 P43187 257675827 91 GLQFEDFISLHQSLN 15 3324.0357 Birch 3505 Bet v 3 P43187 257675827 96 DFISLHQSLNDSYFA 15 3324.0365 Birch 3505 Bet v 3 P43187 257675827 146 GDGYISARELQMVLG 15 3324.0366 Birch 3505 Bet v 3 P43187 257675827 171 DRVEKMIVSVDSNRD 15 3324.0362 Birch 3505 Bet v 3 P43187 257675827 191 FEFKDMMRSVLVRSS 15 3324.0363 Birch 3505 Bet v 4 Q39419 2051993 31 ELGEALKTLGSITPD 15 3324.0367 Birch 3505 Bet v 4 Q39419 2051993 61 FISFQEFTDFGRANR 15 3324.0368 Birch 3505 Bet v 4 Q39419 2051993 71 GRANRGLLKDVAKIF 15 3337.0017 Birch 3505 Bet v 6 Q9FUW6 10764491 16 IGKFIVEASAKSGHP 15 3324.0375 Birch 3505 Bet v 6 Q9FUW6 10764491 46 GKLVEKFKGLGVTLL 15 3324.0374 Birch 3505 Bet v 6 Q9FUW6 10764491 51 KFKGLGVTLLHGDLY 15 3324.0376 Birch 3505 Bet v 6 Q9FUW6 10764491 71 VKAFKQVDVVISTVG 15 3324.0384 Birch 3505 Bet v 6 Q9FUW6 10764491 76 QVDVVISTVGHLQLA 15 3324.0389 Birch 3505 Bet v 6 Q9FUW6 10764491 86 HLQLADQVKIIAAIK 15 3324.0381 Birch 3505 Bet v 6 Q9FUW6 10764491 91 DQVKIIAAIKEAGNI 15 3324.0377 Birch 3505 Bet v 6 Q9FUW6 10764491 101 EAGNIKRFFPSEFGN 15 3324.0385 Birch 3505 Bet v 6 Q9FUW6 10764491 141 EAEGIPYTYVSSNFF 15 3324.0370 Birch 3505 Bet v 6 Q9FUW6 10764491 146 PYTYVSSNFFAGYFL 15 3324.0383 Birch 3505 Bet v 6 Q9FUW6 10764491 151 SSNFFAGYFLPTLAQ 15 3324.0373 Birch 3505 Bet v 6 Q9FUW6 10764491 156 AGYFLPTLAQPGLTS 15 3324.0388 Birch 3505 Bet v 6 Q9FUW6 10764491 206 RTLNKIVYIKPAKNI 15 3324.0387 Birch 3505 Bet v 6 Q9FUW6 10764491 211 IVYIKPAKNIYSFNE 15 3324.0386 Birch 3505 Bet v 6 Q9FUW6 10764491 216 PAKNIYSFNEIVALW 15 3324.0372 Birch 3505 Bet v 6 Q9FUW6 10764491 221 YSFNEIVALWEKKIG 15 3324.0371 Birch 3505 Bet v 6 Q9FUW6 10764491 256 PIPINVILAINHSVF 15 3324.0369 Birch 3505 Bet v 6 Q9FUW6 10764491 261 VILAINHSVFVKGDH 15 3324.0380 Birch 3505 Bet v 6 Q9FUW6 10764491 276 TNFEIEASFGVEASE 15 3324.0379 Birch 3505 Bet v 6 Q9FUW6 10764491 291 LYPDVKYTTVEEYLQ 15 3324.0382 Birch 3505 Bet v 6 Q9FUW6 10764491 294 DVKYTTVEEYLQQFV 15 3324.0378 Birch 3505 Bet v 7 P81531(IUIS) 21431524 51 GKPLHYKKSSFHRVI 15 3324.0390 Birch 3505 Bet v 7 P81531(IUIS) 21431524 101 TGPGILSMANAGPGT 15 3337.0018 Birch 3505 Bet v 7 P81531(IUIS) 21431524 116 NGSQFFICTAKTEWL 15 3324.0391 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 6 KCIFHTFSLTMARLA 15 3324.1111 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 11 TFSLTMARLATLAAL 15 3324.1110 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 16 MARLATLAALLVALL 15 3324.1108 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 21 TLAALLVALLFVANA 15 3324.1109 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 26 LVALLFVANAAAFRT 15 3324.1107 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 31 FVANAAAFRTTITTM 15 3324.1112 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 36 AAFRTTITTMEIDED 15 3324.1114 Black walnut 16719 Jug n 1 Q7Y1C2 31321942 71 LNHCQYYLRQQSRSG 15 3324.1113 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 66 RHNPYYFHSQSIRSR 15 3324.1119 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 86 GEVKYLERFAERTEL 15 3324.1122 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 96 ERTELLRGIENYRVV 15 3324.1121 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 101 LRGIENYRVVILDAN 15 3324.1118 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 106 NYRVVILDANPNTFM 15 3324.1125 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 191 GQVREYYAAGAKSPD 15 3324.1126 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 206 QSYLRVFSNDILVAA 15 3324.1115 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 211 VFSNDILVAALNTPR 15 3324.1116 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 301 LQEMDVLVNYAEIKR 15 3324.1123 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 366 TGRFQKVTARLARGD 15 3324.1124 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 381 IFVIPAGHPIAITAS 15 3324.1128 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 411 NNQRNFLAGQNSIIN 15 3324.1117 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 416 FLAGQNSIINQLERE 15 3324.1120 Black walnut 16719 Jug n 2 Q7Y1C1 31321944 446 IFESQMESYFVPTER 15 3324.1127 Canary grass 28479 Pha a 1 Q41260 2498576 1 MMKMVCSSSSSSLLV 15 3324.1430 Canary grass 28479 Pha a 1 Q41260 2498576 11 SSLLVVAALLAVFVG 15 3324.1426 Canary grass 28479 Pha a 1 Q41260 2498576 16 VAALLAVFVGSAQGI 15 3324.1427 Canary grass 28479 Pha a 1 Q41260 2498576 126 EEPIAPYHFDLSGHA 15 3324.1429 Canary grass 28479 Pha a 1 Q41260 2498576 186 PNYLALLVKYVDGDG 15 3324.1428 Canary grass 28479 Pha a 1 Q41260 2498576 221 SWGAIWRIDTPDKLT 15 3337.0039 Canary grass 28479 Pha a 5 P56164 1246117 1 MAVQKYTMALFLAVA 15 3324.1434 Canary grass 28479 Pha a 5 P56164 1246117 6 YTMALFLAVALVAGP 15 3324.1431 Canary grass 28479 Pha a 5 P56164 1246117 11 FLAVALVAGPAAPTP 15 3324.1438 Canary grass 28479 Pha a 5 P56164 1246117 101 DAAYRVAYEAAEGST 15 3324.1443 Canary grass 28479 Pha a 5 P56164 1246117 116 PEAKYDAFIAALTEA 15 3324.1435 Canary grass 28479 Pha a 5 P56164 1246117 121 DAFIAALTEALRVIA 15 3324.1432 Canary grass 28479 Pha a 5 P56164 1246117 126 ALTEALRVIAGAFEV 15 3324.1437 Canary grass 28479 Pha a 5 P56164 1246117 161 VDKIDAAFKIAATAA 15 3324.1440 Canary grass 28479 Pha a 5 P56164 1246117 166 AAFKIAATAANSAPA 15 3324.1441 Canary grass 28479 Pha a 5 P56164 1246117 181 NDKFTVFEGAFNKAI 15 3324.1442 Canary grass 28479 Pha a 5 P56164 1246117 201 GAYETYKFIPSLEAA 15 3324.1433 Canary grass 28479 Pha a 5 P56164 1246117 206 YKFIPSLEAAVKQAY 15 3324.1436 Canary grass 28479 Pha a 5 P56164 1246117 216 VKQAYGATVARAPEV 15 3324.1444 Canary grass 28479 Pha a 5 P56164 1246117 226 RAPEVKYAVFEAGLT 15 3324.1439 Cat epithelia 9685 Fel d 1 1 P30438 146387241 1 MKGACVLVLLWAALL 15 3324.1047 Cat epithelia 9685 Fel d 1 1 P30438 146387241 6 VLVLLWAALLLISGG 15 3324.1046 Cat epithelia 9685 Fel d 1 1 P30438 146387241 41 DEYVEQVAQYKALPV 15 3324.1049 Cat epithelia 9685 Fel d 1 1 P30438 146387241 46 QVAQYKALPVVLENA 15 3324.1051 Cat epithelia 9685 Fel d 1 1 P30438 146387241 51 KALPVVLENARILKN 15 3324.1048 Cat epithelia 9685 Fel d 1 1 P30438 146387241 78 NALSVLDKIYTSPLC 15 3324.1050 Cat epithelia 9685 Fel d 1 2 P30440 146387241 1 MRGALLVLALLVTQA 15 3324.1054 Cat epithelia 9685 Fel d 1 2 P30440 146387241 6 LVLALLVTQALGVKM 15 3324.1053 Cat epithelia 9685 Fel d 1 2 P30440 146387241 21 AETCPIFYDVFFAVA 15 3324.1056 Cat epithelia 9685 Fel d 1 2 P30440 146387241 26 IFYDVFFAVANGNEL 15 3324.1052 Cat epithelia 9685 Fel d 1 2 P30440 146387241 36 NGNELLLDLSLTKVN 15 3324.1055 Cat epithelia 9685 Fel d 1 2 P30440 146387241 66 YVENGLISRVLDGLV 15 3324.1057 Cat epithelia 9685 Fel d 2 P49064 30962111 1 MKWVTFISLLLLFSS 15 3324.1058 Cat epithelia 9685 Fel d 2 P49064 30962111 6 FISLLLLFSSAYSRG 15 3324.1063 Cat epithelia 9685 Fel d 2 P49064 30962111 41 EHFRGLVLVAFSQYL 15 3324.1060 Cat epithelia 9685 Fel d 2 P49064 30962111 46 LVLVAFSQYLQQCPF 15 3324.1061 Cat epithelia 9685 Fel d 2 P49064 30962111 61 EDHVKLVNEVTEFAK 15 3324.1075 Cat epithelia 9685 Fel d 2 P49064 30962111 156 QRFLGKYLYEIARRH 15 3324.1069 Cat epithelia 9685 Fel d 2 P49064 30962111 161 KYLYEIARRHPYFYA 15 3324.1068 Cat epithelia 9685 Fel d 2 P49064 30962111 166 IARRHPYFYAPELLY 15 3324.1070 Cat epithelia 9685 Fel d 2 P49064 30962111 171 PYFYAPELLYYAEEY 15 3324.1078 Cat epithelia 9685 Fel d 2 P49064 30962111 231 GERAFKAWSVARLSQ 15 3324.1059 Cat epithelia 9685 Fel d 2 P49064 30962111 246 KFPKAEFAEISKLVT 15 3324.1077 Cat epithelia 9685 Fel d 2 P49064 30962111 346 AKDVFLGTFLYEYSR 15 3324.1074 Cat epithelia 9685 Fel d 2 P49064 30962111 351 LGTFLYEYSRRHPEY 15 3324.1076 Cat epithelia 9685 Fel d 2 P49064 30962111 361 RHPEYSVSLLLRLAK 15 3324.1062 Cat epithelia 9685 Fel d 2 P49064 30962111 366 SVSLLLRLAKEYEAT 15 3324.1071 Cat epithelia 9685 Fel d 2 P49064 30962111 396 HVFDEFKPLVEEPHN 15 3324.1072 Cat epithelia 9685 Fel d 2 P49064 30962111 421 KLGEYGFQNALLVRY 15 3324.1065 Cat epithelia 9685 Fel d 2 P49064 30962111 426 GFQNALLVRYTKKVP 15 3324.1080 Cat epithelia 9685 Fel d 2 P49064 30962111 471 SCAEDYLSVVLNRLC 15 3324.1064 Cat epithelia 9685 Fel d 2 P49064 30962111 476 YLSVVLNRLCVLHEK 15 3324.1066 Cat epithelia 9685 Fel d 2 P49064 30962111 506 VNRRPCFSALQVDET 15 3324.1081 Cat epithelia 9685 Fel d 2 P49064 30962111 531 FTFHADLCTLPEAEK 15 3324.1079 Cat epithelia 9685 Fel d 2 P49064 30962111 546 QIKKQSALVELLKHK 15 3324.1073 Cat epithelia 9685 Fel d 2 P49064 30962111 594 EEGPKLVAAAQAALA 15 3324.1067 Cat epithelia 9685 Fel d 3 Q8WNR9 17939981 31 TNETYQKFEAIEYKT 15 3324.1087 Cat epithelia 9685 Fel d 3 Q8WNR9 17939981 41 IEYKTQVVAGINYYI 15 3324.1083 Cat epithelia 9685 Fel d 3 Q8WNR9 17939981 46 QVVAGINYYIKVQVD 15 3324.1086 Cat epithelia 9685 Fel d 3 Q8WNR9 17939981 51 INYYIKVQVDDNRYI 15 3324.1084 Cat epithelia 9685 Fel d 3 Q8WNR9 17939981 61 DNRYIHIKVFKGLPV 15 3324.1082 Cat epithelia 9685 Fel d 3 Q8WNR9 17939981 66 HIKVFKGLPVQDSSL 15 3324.1085 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 1 MKLLLLCLGLILVCA 15 3324.1096 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 31 ISGEWYSILLASDVK 15 3324.1088 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 36 YSILLASDVKEKIEE 15 3324.1094 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 51 NGSMRVFVEHIKALD 15 3324.1098 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 56 VFVEHIKALDNSSLS 15 3324.1090 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 61 IKALDNSSLSFVFHT 15 3324.1091 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 96 YTVVYDGYNVFSIVE 15 3324.1100 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 101 DGYNVFSIVETVYDE 15 3324.1097 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 106 FSIVETVYDEYILLH 15 3324.1095 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 111 TVYDEYILLHLLNFD 15 3324.1089 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 116 YILLHLLNFDKTRPF 15 3324.1093 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 121 LLNFDKTRPFQLVEF 15 3324.1092 Cat epithelia 9685 Fel d 4 Q5VFH6 45775300 126 KTRPFQLVEFYAREP 15 3324.1099 Cladosporium 29918 Cla h 10 P40108 291047712 16 QPTGLFINNEFVKGQ 15 3324.0559 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 56 DVDIAVAAARKAFEG 15 3324.0561 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 81 GKLLNNLANLFEKNI 15 3324.0552 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 86 NLANLFEKNIDLLAA 15 3324.0565 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 91 FEKNIDLLAAVESLD 15 3324.0555 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 166 PLLMWAWKIGPAIAC 15 3324.0558 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 191 QTPLGGLVAASLVKE 15 3324.0557 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 246 RTILKAAASSNLKKV 15 3324.0566 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 281 AISWVNFGIFFNHGQ 15 3324.0553 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 341 SKVQFDRIMEYIQAG 15 3324.0560 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 371 DKGYFIEPTIFSNVT 15 3324.0554 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 386 EDMKIVKEEIFGPVC 15 3324.0556 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 416 NASTYGLAAAVHTKN 15 3324.0564 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 431 LNTAIEVSNALKAGT 15 3324.0563 herbarum Cladosporium 29918 Cla h 10 P40108 291047712 441 LKAGTVWVNTYNTLH 15 3324.0562 herbarum Cladosporium 29918 Cla h 12 P50344 1710589 1 MSAAELASSYAALIL 15 3324.0569 herbarum Cladosporium 29918 Cla h 12 P50344 1710589 6 LASSYAALILADEGL 15 3324.0567 herbarum Cladosporium 29918 Cla h 12 P50344 1710589 21 EITADKLQALISAAK 15 3324.0568 herbarum Cladosporium 29918 Cla h 12 P50344 1710589 26 KLQALISAAKVPEIE 15 3324.0570 herbarum Cladosporium 29918 Cla h 12 P50344 1710589 36 VPEIEPIWTSLFAKA 15 3324.0571 herbarum Cladosporium 29918 Cla h 12 P50344 1710589 41 PIWTSLFAKALEGKD 15 3324.0572 herbarum Cladosporium 29918 Cla h 5 P42039 5777795 1 MKYLAAFLLLGLAGN 15 3324.0573 herbarum Cladosporium 29918 Cla h 5 P42039 5777795 6 AFLLLGLAGNSSPSA 15 3324.0576 herbarum Cladosporium 29918 Cla h 5 P42039 5777795 21 EDIKTVLSSVGIDAD 15 3324.0574 herbarum Cladosporium 29918 Cla h 5 P42039 5777795 46 LEGKDINELISSGSE 15 3324.0577 herbarum Cladosporium 29918 Cla h 5 P42039 5777795 51 INELISSGSEKLASV 15 3324.0575 herbarum Cladosporium 29918 Cla h 6 P42040 467660 1 MPISKIHSRYVYDSR 15 3324.0587 herbarum Cladosporium 29918 Cla h 6 P42040 467660 66 VANVNEIIAPALIKE 15 3324.0584 herbarum Cladosporium 29918 Cla h 6 P42040 467660 106 IGANAILGVSMAVAK 15 3324.0579 herbarum Cladosporium 29918 Cla h 6 P42040 467660 111 ILGVSMAVAKAAAAE 15 3324.0586 herbarum Cladosporium 29918 Cla h 6 P42040 467660 126 KRVPLYAHISDLSGT 15 3324.0590 herbarum Cladosporium 29918 Cla h 6 P42040 467660 161 GGRLAFQEFMIVPSG 15 3324.0588 herbarum Cladosporium 29918 Cla h 6 P42040 467660 166 FQEFMIVPSGAPSFT 15 3324.0581 herbarum Cladosporium 29918 Cla h 6 P42040 467660 186 GAEVYQKLKSLTKKR 15 3324.0585 herbarum Cladosporium 29918 Cla h 6 P42040 467660 241 IKIAMDVASSEFYKA 15 3324.0589 herbarum Cladosporium 29918 Cla h 6 P42040 467660 271 KSKWITYEQLADQYK 15 3324.0594 herbarum Cladosporium 29918 Cla h 6 P42040 467660 281 ADQYKQLAAKYPIVS 15 3324.0593 herbarum Cladosporium 29918 Cla h 6 P42040 467660 306 EAWSYFYKTSGSDFQ 15 3324.0580 herbarum Cladosporium 29918 Cla h 6 P42040 467660 341 KACNALLLKVNQIGT 15 3324.0578 herbarum Cladosporium 29918 Cla h 6 P42040 467660 346 LLLKVNQIGTITEAI 15 3324.0591 herbarum Cladosporium 29918 Cla h 6 P42040 467660 366 SFAAGWGVMVSHRSG 15 3324.0583 herbarum Cladosporium 29918 Cla h 6 P42040 467660 386 TIADIVVGLRAGQIK 15 3324.0592 herbarum Cladosporium 29918 Cla h 6 P42040 467660 411 AKLNQILRIEEELGD 15 3324.0582 herbarum Cladosporium 29918 Cla h 7 P42059 1168970 1 MAPKIAIIFYSTWGH 15 3324.0597 herbarum Cladosporium 29918 Cla h 7 P42059 1168970 131 AMSTLSHHGIIYVPL 15 3337.0019 herbarum Cladosporium 29918 Cla h 7 P42059 1168970 141 IYVPLGYKTTFHLLG 15 3324.0595 herbarum Cladosporium 29918 Cla h 7 P42059 1168970 190 QGKAFYEAVAKVNFQ 15 3324.0596 herbarum Cladosporium 29918 Cla h 8 P0C0Y5 296863443 46 GAAVAITYASRAQGA 15 3324.0600 herbarum Cladosporium 29918 Cla h 8 P0C0Y5 296863443 101 QIDAFIANAGATADS 15 3324.0601 herbarum Cladosporium 29918 Cla h 8 P0C0Y5 296863443 151 GTGSLVITASMSGHI 15 3324.0603 herbarum Cladosporium 29918 Cla h 8 P0C0Y5 296863443 181 GCIHMARSLANEWRD 15 3324.0602 herbarum Cladosporium 29918 Cla h 8 P0C0Y5 296863443 236 KELKGAYVYFASDAS 15 3324.0598 herbarum Cladosporium 29918 Cla h 8 P0C0Y5 296863443 241 AYVYFASDASTYTTG 15 3324.0599 herbarum Cypress 13415 Cha o 1 Q96385 219928905 1 MASCTLLAVLVFLCA 15 3324.0519 Cypress 13415 Cha o 1 Q96385 219928905 6 LLAVLVFLCAIVSCF 15 3324.0518 Cypress 13415 Cha o 1 Q96385 219928905 86 ERSLWIIFSKNLNIK 15 3324.0517 Cypress 13415 Cha o 1 Q96385 219928905 91 IIFSKNLNIKLNMPL 15 3324.0522 Cypress 13415 Cha o 1 Q96385 219928905 96 NLNIKLNMPLYIAGN 15 3324.0523 Cypress 13415 Cha o 1 Q96385 219928905 126 GPCLFMRTVSHVILH 15 3324.0520 Cypress 13415 Cha o 1 Q96385 219928905 131 MRTVSHVILHGLNIH 15 3324.0528 Cypress 13415 Cha o 1 Q96385 219928905 151 VSGNVLISEASGVVP 15 3324.0526 Cypress 13415 Cha o 1 Q96385 219928905 196 LVDVTLASTGVTISN 15 3324.0524 Cypress 13415 Cha o 1 Q96385 219928905 211 NHFFNHHKVMLLGHS 15 3324.0521 Cypress 13415 Cha o 1 Q96385 219928905 231 DKSMKVTVAFNQFGP 15 3324.0525 Cypress 13415 Cha o 1 Q96385 219928905 256 YGLIHVANNNYDPWS 15 3324.0527 Cypress 13415 Cha o 2 Q7M1E7 47606004 1 MGMKFMAAVAFLALQ 15 3324.0531 Cypress 13415 Cha o 2 Q7M1E7 47606004 6 MAAVAFLALQLIVMA 15 3324.0529 Cypress 13415 Cha o 2 Q7M1E7 47606004 11 FLALQLIVMAAAEDQ 15 3324.0532 Cypress 13415 Cha o 2 Q7M1E7 47606004 31 LDSDIEQYLRSNRSL 15 3324.0544 Cypress 13415 Cha o 2 Q7M1E7 47606004 36 EQYLRSNRSLKKLVH 15 3324.0538 Cypress 13415 Cha o 2 Q7M1E7 47606004 76 EAFATTWNAACKKAS 15 3324.0551 Cypress 13415 Cha o 2 Q7M1E7 47606004 81 TWNAACKKASAVLLV 15 3324.0547 Cypress 13415 Cha o 2 Q7M1E7 47606004 86 CKKASAVLLVPANKK 15 3324.0534 Cypress 13415 Cha o 2 Q7M1E7 47606004 96 PANKKFFVNNLVFRG 15 3324.0533 Cypress 13415 Cha o 2 Q7M1E7 47606004 131 ARWKNSKIWLQFAQL 15 3324.0542 Cypress 13415 Cha o 2 Q7M1E7 47606004 136 SKIWLQFAQLTDFNL 15 3324.0530 Cypress 13415 Cha o 2 Q7M1E7 47606004 181 NRPTAIKIDYSKSVT 15 3324.0545 Cypress 13415 Cha o 2 Q7M1E7 47606004 186 IKIDYSKSVTVKELT 15 3324.0535 Cypress 13415 Cha o 2 Q7M1E7 47606004 196 VKELTLMNSPEFHLV 15 3324.0550 Cypress 13415 Cha o 2 Q7M1E7 47606004 236 IDIFASKRFHIEKCV 15 3324.0539 Cypress 13415 Cha o 2 Q7M1E7 47606004 296 HVHVNRAKFIDTQNG 15 3324.0543 Cypress 13415 Cha o 2 Q7M1E7 47606004 321 GLASYITYENVEMIN 15 3324.0537 Cypress 13415 Cha o 2 Q7M1E7 47606004 331 VEMINSENPILINQF 15 3324.0549 Cypress 13415 Cha o 2 Q7M1E7 47606004 336 SENPILINQFYCTSA 15 3324.0540 Cypress 13415 Cha o 2 Q7M1E7 47606004 366 TYKNIHGTSATAAAI 15 3324.0548 Cypress 13415 Cha o 2 Q7M1E7 47606004 376 TAAAIQLMCSDSVPC 15 3324.0536 Cypress 13415 Cha o 2 Q7M1E7 47606004 391 TGIQLSNVSLKLTSG 15 3324.0541 Cypress 13415 Cha o 2 Q7M1E7 47606004 481 QPCKPKLIIVHPNKP 15 3324.0546 Cypress 49011 Cup a 1 Q9SCG9 9087167 66 KALWIIFSQNMNIKL 15 3324.0604 Cypress 49011 Cup a 1 Q9SCG9 9087167 71 IFSQNMNIKLQMPLY 15 3324.0607 Cypress 49011 Cup a 1 Q9SCG9 9087167 76 MNIKLQMPLYVAGYK 15 3324.0613 Cypress 49011 Cup a 1 Q9SCG9 9087167 106 PCLFMRKASHVILHG 15 3324.0608 Cypress 49011 Cup a 1 Q9SCG9 9087167 131 LGDVLVSESIGVEPV 15 3324.0610 Cypress 49011 Cup a 1 Q9SCG9 9087167 186 TISNNHFFNHHKVML 15 3324.0612 Cypress 49011 Cup a 1 Q9SCG9 9087167 191 HFFNHHKVMLLGHDD 15 3324.0605 Cypress 49011 Cup a 1 Q9SCG9 9087167 211 KSMKVTVAFNQFGPN 15 3324.0609 Cypress 49011 Cup a 1 Q9SCG9 9087167 231 PRARYGLVHVANNNY 15 3324.0611 Cypress 49011 Cup a 1 Q9SCG9 9087167 316 EDTNIYNSNEAFKVE 15 3324.0606 Cypress 13469 Cup s 1 Q9M4S2 257321241 1 MDSPCLIAVLVFLCA 15 3324.0617 Cypress 13469 Cup s 1 Q9M4S2 257321241 6 LIAVLVFLCAIVSCY 15 3324.0614 Cypress 13469 Cup s 1 Q9M4S2 257321241 11 VFLCAIVSCYSDNPI 15 3324.0625 Cypress 13469 Cup s 1 Q9M4S2 257321241 86 EKALWIIFSQNMNIK 15 3324.0615 Cypress 13469 Cup s 1 Q9M4S2 257321241 91 IIFSQNMNIKLKMPL 15 3324.0618 Cypress 13469 Cup s 1 Q9M4S2 257321241 96 NMNIKLKMPLYVAGH 15 3324.0624 Cypress 13469 Cup s 1 Q9M4S2 257321241 126 GPCLFMRKVSHVILH 15 3324.0619 Cypress 13469 Cup s 1 Q9M4S2 257321241 131 MRKVSHVILHGLHIH 15 3324.0621 Cypress 13469 Cup s 1 Q9M4S2 257321241 151 VLGNVLVSESIGVEP 15 3324.0627 Cypress 13469 Cup s 1 Q9M4S2 257321241 211 NHFFNHHKVMLLGHD 15 3324.0616 Cypress 13469 Cup s 1 Q9M4S2 257321241 231 DKSMKVTVAFNQFGP 15 3324.0622 Cypress 13469 Cup s 1 Q9M4S2 257321241 256 YGLVHVANNNYDQWN 15 3324.0626 Cypress 13469 Cup s 1 Q9M4S2 257321241 336 TEETNIYTSNEAFKV 15 3324.0620 Cypress 13469 Cup s 1 Q9M4S2 257321241 346 EAFKVENGNLAPQLT 15 3324.0623 Cypress 13469 Cup s 3 Q69CS2 38456226 1 MARVSELALLLVATS 15 3324.0629 Cypress 13469 Cup s 3 Q69CS2 38456226 6 ELALLLVATSAISLH 15 3324.0628 Cypress 13469 Cup s 3 Q69CS2 38456226 11 LVATSAISLHMQEAG 15 3324.0630 Cypress 13469 Cup s 3 Q69CS2 38456226 56 TWTVNLAAGTASARF 15 3324.0632 Cypress 13469 Cup s 3 Q69CS2 38456226 116 DYYDVSLVDGFNIPL 15 3337.0020 Cypress 13469 Cup s 3 Q69CS2 38456226 121 SLVDGFNIPLAINPT 15 3324.0631 Date palm 42345 Pho d 2 Q8L5D8 21322677 36 SSSFPQFKSEEITNI 15 3324.1549 Date palm 42345 Pho d 2 Q8L5D8 21322677 61 APTGLYLGSTKYMVI 15 3324.1548 Date palm 42345 Pho d 2 Q8L5D8 21322677 66 YLGSTKYMVIQGEPG 15 3324.1546 Date palm 42345 Pho d 2 Q8L5D8 21322677 71 KYMVIQGEPGAVIRG 15 3324.1547 Date palm 42345 Pho d 2 Q8L5D8 21322677 91 GVTVKKTNQALIFGI 15 3324.1550 Dermatophagoides 6954 Der f 1 A1YW11 119633260 1 MKFVLAIASLLVLST 15 3324.0664 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 6 AIASLLVLSTVYARP 15 3324.0665 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 11 LVLSTVYARPASIKT 15 3324.0677 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 26 FEEFKKAFNKNYATV 15 3324.0681 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 46 ARKNFLESLKYVEAN 15 3324.0668 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 61 KGAINHLSDLSLDEF 15 3324.0683 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 71 SLDEFKNRYLMSAEA 15 3324.0672 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 76 KNRYLMSAEAFEQLK 15 3324.0667 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 81 MSAEAFEQLKTQFDL 15 3324.0684 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 86 FEQLKTQFDLNAETS 15 3324.0682 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 131 GSCWAFSGVAATESA 15 3324.0670 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 136 FSGVAATESAYLAYR 15 3324.0666 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 141 ATESAYLAYRNTSLD 15 3324.0674 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 146 YLAYRNTSLDLSEQE 15 3324.0673 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 176 PRGIEYIQQNGVVEE 15 3324.0679 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 221 PDVKQIREALTQTHT 15 3324.0685 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 231 TQTHTAIAVIIGIKD 15 3324.0680 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 241 IGIKDLRAFQHYDGR 15 3324.0676 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 296 GDSGYGYFQAGNNLM 15 3324.0669 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 301 GYFQAGNNLMMIEQY 15 3324.0671 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 306 GNNLMMIEQYPYVVI 15 3324.0675 farinae Dermatophagoides 6954 Der f 1 A1YW11 119633260 307 NNLMMIEQYPYVVIM 15 3324.0678 farinae Dermatophagoides 6954 Der f 10 Q23939 42559584 101 RSEERLKIATAKLEE 15 3324.0686 farinae Dermatophagoides 6954 Der f 10 Q23939 42559584 106 LKIATAKLEEASQSA 15 3324.0689 farinae Dermatophagoides 6954 Der f 10 Q23939 42559584 191 VELEEELRVVGNNLK 15 3324.0690 farinae Dermatophagoides 6954 Der f 10 Q23939 42559584 216 QREEAYEQQIRIMTA 15 3324.0688 farinae Dermatophagoides 6954 Der f 10 Q23939 42559584 221 YEQQIRIMTAKLKEA 15 3324.0687 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 11 KLRKLLEDVHIESEE 15 3324.0719 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 61 KFQAEVFELLAQLET 15 3324.0692 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 66 VFELLAQLETANKEK 15 3324.0696 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 96 ELNIKIEEINRTVIE 15 3324.0713 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 101 IEEINRTVIELTSHK 15 3324.0718 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 131 EVKLQLDNANHLKTQ 15 3324.0716 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 136 LDNANHLKTQIAQQL 15 3324.0699 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 141 HLKTQIAQQLEDTRH 15 3324.0717 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 171 AHTLEVELESLKVQL 15 3324.0709 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 191 ARLELERQLTKANGD 15 3324.0711 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 226 ELRRKMAQKISEYEE 15 3324.0714 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 236 SEYEEQLEALLNKCS 15 3324.0710 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 256 KSRLQSEVEVLIMDL 15 3324.0720 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 261 SEVEVLIMDLEKATR 15 3324.0706 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 281 EKRVAQLEKINLDLK 15 3324.0697 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 311 ELRVKIAELQKLQHE 15 3324.0715 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 316 IAELQKLQHEYEKLR 15 3324.0704 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 376 DELSAAYKEAETLRK 15 3324.0703 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 396 NQRLIAELAQVRHDY 15 3324.0698 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 421 IEALRKQYQIEIEQL 15 3324.0693 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 426 KQYQIEIEQLNMRLA 15 3324.0695 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 431 EIEQLNMRLAEAEAK 15 3324.0712 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 451 ARLKKKYQAQITELE 15 3324.0691 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 456 KYQAQITELELSLDA 15 3324.0721 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 481 TIKKQALQITELQAH 15 3324.0702 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 521 QAELEEMRIALEQAN 15 3324.0701 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 531 LEQANRAKRQAEQLH 15 3324.0722 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 556 TTINVNLASAKSKLE 15 3324.0708 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 566 KSKLESEFSALQADY 15 3324.0705 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 596 KLTIELKSTKDLLIE 15 3324.0694 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 601 LKSTKDLLIEEQERL 15 3324.0700 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 616 VKLETVKKSLEQEVR 15 3324.0723 farinae Dermatophagoides 6954 Der f 11 Q967Z0 42559514 631 TLHVRIEEVEANALA 15 3324.0707 farinae Dermatophagoides 6954 Der f 13 Q1M2P5 37958167 6 GKYKLEKSEKFDEFL 15 3324.0727 farinae Dermatophagoides 6954 Der f 13 Q1M2P5 37958167 26 GFMVKTAAKTLKPTF 15 3324.0725 farinae Dermatophagoides 6954 Der f 13 Q1M2P5 37958167 46 NDQYIFRSLSTFKNT 15 3324.0724 farinae Dermatophagoides 6954 Der f 13 Q1M2P5 37958167 51 FRSLSTFKNTEAKFK 15 3324.0726 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 16 TRRYVAELTAVGSPS 15 3324.0735 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 51 KSPEHEFNTEFTIHA 15 3324.0731 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 91 DKENNVRKNQLNLQY 15 3324.0737 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 96 VRKNQLNLQYKFAGD 15 3324.0734 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 116 VDYENEFSFNLKRSS 15 3324.0738 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 141 AKYMSSHFPILNHKV 15 3324.0736 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 146 SHFPILNHKVNIQFK 15 3324.0733 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 151 LNHKVNIQFKYRPFK 15 3324.0730 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 156 NIQFKYRPFKVNELN 15 3324.0732 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 181 HKFQLMRNSQIEVEE 15 3324.0728 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 196 VRPFKMHGNSDIKLM 15 3324.0739 farinae Dermatophagoides 6954 Der f 14 Q94507 1545803 311 KIFINHKSEMTKPTN 15 3324.0729 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 1 MKTIYAILSIMACIG 15 3324.0740 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 6 AILSIMACIGLMNAS 15 3324.0744 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 11 MACIGLMNASIKRDH 15 3324.0746 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 91 SWEKRGYERFNNLRL 15 3324.0747 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 96 GYERFNNLRLKNPEL 15 3324.0743 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 106 KNPELTTMISLGGWY 15 3324.0753 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 131 ANPTYRQQFIQSVLD 15 3324.0755 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 136 RQQFIQSVLDFLQEY 15 3324.0741 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 171 IDKQNYLALVRELKD 15 3324.0745 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 176 YLALVRELKDAFEPH 15 3324.0756 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 186 AFEPHGYLLTAAVSP 15 3324.0754 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 231 GWENFYGHNAPLYKR 15 3324.0748 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 251 ELHTYFNVNYTMHYY 15 3324.0742 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 256 FNVNYTMHYYLNNGA 15 3324.0750 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 316 LSYIELCQLFQKEEW 15 3324.0749 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 361 KLAFLKELGVSGVMV 15 3324.0751 farinae Dermatophagoides 6954 Der f 15 Q9U6R7 21444331 391 NPLLNKVHNMINGDE 15 3324.0752 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 6 KNFDVIPIGHTFFFI 15 3324.0777 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 11 IPIGHTFFFIWRIKQ 15 3324.0772 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 16 TFFFIWRIKQFELVP 15 3324.0761 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 21 WRIKQFELVPVPKED 15 3324.0759 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 111 EIEEFESRQFSSYFK 15 3324.0774 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 121 SSYFKNGIIYLKGGY 15 3324.0760 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 171 VMNNGDVFILLVPNF 15 3324.0768 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 176 DVFILLVPNFVFVWT 15 3324.0757 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 181 LVPNFVFVWTGKHSN 15 3324.0773 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 211 SELNRFKLSSVILED 15 3324.0758 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 216 FKLSSVILEDGKEVE 15 3324.0767 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 236 EYDAFNKALSLDKKD 15 3324.0775 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 291 ISFVKNGPLSRADLD 15 3324.0776 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 336 IKYAMELINKKKYPN 15 3324.0771 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 361 DESVEFKSLFESWQM 15 3324.0770 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 366 FKSLFESWQMSEQEK 15 3324.0763 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 376 SEQEKITSARLFRVS 15 3324.0769 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 381 ITSARLFRVSRNGIF 15 3324.0764 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 416 VMDKIYVWIGNQFAE 15 3324.0766 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 421 YVWIGNQFAERIADE 15 3324.0762 farinae Dermatophagoides 6954 Der f 16 Q8MVU3 21591547 451 SGRKFQPNQIIKLKQ 15 3324.0765 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 1 MTRFSLTVLAVLAAC 15 3324.0778 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 6 LTVLAVLAACFGSNI 15 3324.0779 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 56 SLCTHIVYSYFGIDA 15 3324.0786 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 61 IVYSYFGIDAATHEI 15 3324.0780 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 71 ATHEIKLLDEYLMKD 15 3324.0783 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 121 EHYRETFVVSTVDLM 15 3324.0787 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 126 TFVVSTVDLMTRYGF 15 3324.0790 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 171 HTSFVMGVTLPATIA 15 3324.0789 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 196 SNYVDFMNVLSLDYT 15 3324.0781 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 241 HKMVMAVPFYARTWI 15 3324.0792 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 246 AVPFYARTWILEKMN 15 3324.0785 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 281 DGFLSYNELCVQIQA 15 3324.0782 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 306 HDNTAIYAVYVHSNH 15 3324.0791 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 311 IYAVYVHSNHAEWIS 15 3324.0788 farinae Dermatophagoides 6954 Der f 18 Q86R84 27550039 366 PLLHAIQSNYYHGVV 15 3324.0784 farinae Dermatophagoides 6954 Der f 2 Q00855 83755018 1 MISKILCLSLLVAAV 15 3324.0794 farinae Dermatophagoides 6954 Der f 2 Q00855 83755018 6 LCLSLLVAAVVADQV 15 3324.0793 farinae Dermatophagoides 6954 Der f 2 Q00855 83755018 51 PFTLEALFDANQNTK 15 3324.0795 farinae Dermatophagoides 6954 Der f 2 Q00855 83755018 101 QQYDIKYTWNVPKIA 15 3324.0796 farinae Dermatophagoides 6954 Der f 2 Q00855 83755018 121 VVVTVKLIGDNGVLA 15 3337.0026 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 1 MMILTIVVLLAANIL 15 3324.0798 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 6 IVVLLAANILATPIL 15 3324.0797 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 11 AANILATPILPSSPN 15 3324.0803 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 36 AGDCPYQISLQSSSH 15 3324.0804 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 41 YQISLQSSSHFCGGS 15 3324.0802 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 56 ILDEYWILTAAHCVN 15 3324.0800 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 76 KLSIRYNTLKHASGG 15 3324.0799 farinae Dermatophagoides 6954 Der f 3 P49275 1311457 111 NDVALIKLKTPMTLD 15 3324.0801 farinae Dermatophagoides 6954 Der f 6 P49276 14424450 1 MIKIFLVTILIVITV 15 3324.0805 farinae Dermatophagoides 6954 Der f 6 P49276 14424450 6 LVTILIVITVTVDAR 15 3324.0807 farinae Dermatophagoides 6954 Der f 6 P49276 14424450 26 QPKWAYLDSNEFPRS 15 3324.0810 farinae Dermatophagoides 6954 Der f 6 P49276 14424450 61 APFQISLLKDYLIMK 15 3324.0806 farinae Dermatophagoides 6954 Der f 6 P49276 14424450 66 SLLKDYLIMKRHMCG 15 3324.0811 farinae Dermatophagoides 6954 Der f 6 P49276 14424450 141 KTIIILPNPVVPSTN 15 3324.0809 farinae Dermatophagoides 6954 Der f 6 P49276 14424450 265 TRPKYYLDWITKNIV 15 3324.0808 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 1 MMKFLLIAAVAFVAV 15 3324.0812 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 6 LIAAVAFVAVSADPI 15 3324.0813 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 11 AFVAVSADPIHYDKI 15 3324.0818 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 111 IVSMEYDLAYKLGDL 15 3324.0817 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 131 VISDIQDFVVALSLE 15 3324.0819 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 136 QDFVVALSLEISDEG 15 3324.0814 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 156 SFEVRQFANVVNHIG 15 3324.0815 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 161 QFANVVNHIGGLSIL 15 3324.0822 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 166 VNHIGGLSILDPIFG 15 3324.0821 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 171 GLSILDPIFGVLSDV 15 3324.0820 farinae Dermatophagoides 6954 Der f 7 Q26456 1311689 176 DPIFGVLSDVLTAIF 15 3324.0816 farinae Dermatophagoides 6956 Der p 1 P08176 83754033 1 MKIVLAIASLLALSA 15 3324.0823 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 6 AIASLLALSAVYARP 15 3324.0826 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 11 LALSAVYARPSSIKT 15 3324.0828 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 31 KAFNKSYATFEDEEA 15 3324.0834 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 46 ARKNFLESVKYVQSN 15 3324.0839 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 51 LESVKYVQSNGGAIN 15 3324.0838 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 61 GGAINHLSDLSLDEF 15 3324.0842 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 71 SLDEFKNRFLMSAEA 15 3324.0830 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 76 KNRFLMSAEAFEHLK 15 3324.0824 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 81 MSAEAFEHLKTQFDL 15 3324.0844 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 86 FEHLKTQFDLNAETN 15 3324.0841 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 111 EIDLRQMRTVTPIRM 15 3324.0836 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 131 SCWAFSGVAATESAY 15 3324.0832 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 136 SGVAATESAYLAYRN 15 3324.0829 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 141 TESAYLAYRNQSLDL 15 3324.0825 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 176 RGIEYIQHNGVVQES 15 3324.0831 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 186 VVQESYYRYVAREQS 15 3324.0833 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 211 ISNYCQIYPPNVNKI 15 3324.0837 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 226 REALAQTHSAIAVII 15 3324.0840 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 231 QTHSAIAVIIGIKDL 15 3324.0835 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 296 DNGYGYFAANIDLMM 15 3324.0827 pteronyssinus Dermatophagoides 6956 Der p 1 P08176 83754033 306 IDLMMIEEYPYVVIL 15 3324.0843 pteronyssinus Dermatophagoides 6956 Der p 10 O18416 2440053 101 RSEERLKIATAKLEE 15 3324.0845 pteronyssinus Dermatophagoides 6956 Der p 10 O18416 2440053 106 LKIATAKLEEASQSA 15 3324.0847 pteronyssinus Dermatophagoides 6956 Der p 10 O18416 2440053 191 VELEEELRVVGNNLK 15 3324.0848 pteronyssinus Dermatophagoides 6956 Der p 10 O18416 2440053 221 HEQQIRIMTTKLKEA 15 3324.0846 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 61 AELQIQVMSLSERLE 15 3324.0856 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 146 QKFQAEVFELLSQLE 15 3324.0855 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 151 EVFELLSQLETANKE 15 3324.0850 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 176 LEYTVHELNIKIEEI 15 3324.0873 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 181 HELNIKIEEINRTVI 15 3324.0869 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 216 HEVKLQLDNANHLKQ 15 3324.0859 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 226 NHLKQQIAQQLEDTR 15 3324.0853 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 276 EARLELERQLTKANG 15 3324.0866 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 311 EELRRKMAQKISEYE 15 3324.0867 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 346 QSEVEVLIMDLEKAA 15 3324.0874 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 351 VLIMDLEKAAAHAQQ 15 3324.0876 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 481 KNQRLIAELAQVRHD 15 3324.0851 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 506 EIEALRKQYQIEIEQ 15 3324.0852 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 511 RKQYQIEIEQLNMRL 15 3324.0854 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 516 IEIEQLNMRLAEAEA 15 3324.0871 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 536 IARLKKKYQAQITEL 15 3324.0870 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 541 KKYQAQITELELSLD 15 3324.0860 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 566 KTIKKQALQITELQA 15 3324.0872 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 611 EEMRIALEQASRAKR 15 3324.0858 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 636 VRVNELTTINVNLAS 15 3324.0864 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 641 LTTINVNLASAKSKL 15 3324.0861 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 646 VNLASAKSKLESEFS 15 3324.0877 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 651 AKSKLESEFSALQAD 15 3324.0857 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 681 QKLTIELKSTKDLLI 15 3324.0849 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 686 ELKSTKDLLIEEQER 15 3324.0868 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 701 LVKLETVKKSLEQEV 15 3324.0862 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 716 RTLHVRIEEVEANAL 15 3324.0875 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 731 AGGKRVIAKLESRIR 15 3324.0863 pteronyssinus Dermatophagoides 6956 Der p 11 Q6Y2F9 37778944 836 DQAESNLSFIRAKHR 15 3324.0865 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 31 AQSTYVYSLDAKTVL 15 3324.0886 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 36 VYSLDAKTVLTPRDS 15 3324.0936 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 61 VAFVSDCEAVLRLQN 15 3324.0923 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 66 DCEAVLRLQNVAIDG 15 3324.0912 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 96 FAFGYFNGRILGVCP 15 3324.0914 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 116 DWSLNVKKAIVSSLQ 15 3324.0888 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 121 VKKAIVSSLQALSDG 15 3324.0880 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 236 KPVHMSYVKMMLKQN 15 3324.0926 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 286 EVLKKLCSEITEPQA 15 3324.0913 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 306 FTFQKLVDKLRYLSA 15 3324.0915 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 311 LVDKLRYLSAEETAS 15 3324.0909 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 341 RLRELFLDASAFAAS 15 3324.0879 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 346 FLDASAFAASDGSIR 15 3324.0907 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 371 LSITRSTALFTVAAI 15 3324.0882 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 376 STALFTVAAIKAAPN 15 3324.0878 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 391 KETVQVLLPVIASEK 15 3324.0900 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 406 TIRPMLLGFSVLVRR 15 3324.0902 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 436 DARDAYLARLAVARD 15 3324.0904 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 441 YLARLAVARDASERM 15 3324.0897 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 451 ASERMTIVRALENLN 15 3324.0903 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 456 TIVRALENLNVNTDG 15 3324.0933 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 476 NAMDEIIKSTDAEPA 15 3324.0918 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 486 DAEPAMRAAAVNALP 15 3324.0924 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 536 MSHIKDLFAVKGECM 15 3324.0917 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 551 KNYVLTYVDNLKKSK 15 3324.0891 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 631 LKELVEFQVTQSGFD 15 3324.0883 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 646 RELNNAMSLLEKKSF 15 3324.0932 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 651 AMSLLEKKSFQSVMQ 15 3324.0911 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 656 EKKSFQSVMQFLRDM 15 3324.0905 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 661 QSVMQFLRDMLKMLS 15 3324.0895 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 666 FLRDMLKMLSQIRKN 15 3324.0889 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 671 LKMLSQIRKNADDNH 15 3324.0927 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 736 LDSKLVLPTITGLPL 15 3324.0908 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 746 TGLPLMYKFGDNLVV 15 3324.0922 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 811 MIDMNVQKQEHSLLV 15 3324.0928 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 816 VQKQEHSLLVRFNMK 15 3324.0935 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 836 TVMRFKQSLREKRAT 15 3324.0920 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 901 KEVTALELMLKSETQ 15 3324.0901 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 916 DKTRRYIAEMTAVGS 15 3324.0921 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 971 NLKMHMDLPNVLQAD 15 3324.0929 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 996 NNVRKNRLNLQYKFA 15 3324.0930 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1021 ENEFLFNLKRSSKEK 15 3324.0925 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1041 YRAKYMSSHFPILNH 15 3324.0910 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1051 PILNHKVNVQFKYRP 15 3324.0919 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1061 FKYRPFKVNELNLEG 15 3324.0906 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1081 LQHKFRLMRNSQMEV 15 3324.0892 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1211 DKKIFITHKTEMTKP 15 3324.0887 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1271 LFYENYLTVHKGGKL 15 3324.0885 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1291 RNDRKILLDLDNALS 15 3324.0896 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1296 ILLDLDNALSPREGT 15 3324.0890 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1346 NGKLHLSLIDPSTLS 15 3324.0894 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1351 LSLIDPSTLSLVTKA 15 3324.0893 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1511 GKLEGVLSRKVPSHL 15 3324.0916 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1516 VLSRKVPSHLTLETP 15 3324.0934 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1566 PGVQYKIIGNGKIKD 15 3324.0931 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1636 FDPHRAYYINWISSI 15 3324.0899 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1641 AYYINWISSIRKYIQ 15 3324.0898 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1646 WISSIRKYIQNFIVE 15 3324.0884 pteronyssinus Dermatophagoides 6956 Der p 14 Q8N0N0 20385544 1648 SSIRKYIQNFIVEDH 15 3324.0881 pteronyssinus Dermatophagoides 6956 Der p 2 P49278 21465916 1 MMYKILCLSLLVAAV 15 3324.0937 pteronyssinus Dermatophagoides 6956 Der p 2 P49278 21465916 6 LCLSLLVAAVARDQV 15 3324.0938 pteronyssinus Dermatophagoides 6956 Der p 2 P49278 21465916 46 IHRGKPFQLEAVFEA 15 3324.0939 pteronyssinus Dermatophagoides 6956 Der p 2 P49278 21465916 51 PFQLEAVFEANQNTK 15 3324.0941 pteronyssinus Dermatophagoides 6956 Der p 2 P49278 21465916 101 QQYDIKYTWNVPKIA 15 3324.0940 pteronyssinus Dermatophagoides 6956 Der p 20 DERP20SEQ 188485735 26 LLKKYLTRDVFDQLK 15 3324.0943 pteronyssinus Dermatophagoides 6956 Der p 20 DERP20SEQ 188485735 31 LTRDVFDQLKNKKTD 15 3324.0944 pteronyssinus Dermatophagoides 6956 Der p 20 DERP20SEQ 188485735 71 DAQSYKTFAALFDPI 15 3324.0942 pteronyssinus Dermatophagoides 6956 Der p 20 DERP20SEQ 188485735 76 KTFAALFDPIIDDYH 15 3324.0945 pteronyssinus Dermatophagoides 6956 Der p 20 DERP20SEQ 188485735 131 LNGYPFNPMLTEAQY 15 3324.0946 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 1 MKFIITLFAAIVMAA 15 3324.0947 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 6 TLFAAIVMAAAVSGF 15 3324.0949 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 11 IVMAAAVSGFIVGDK 15 3324.0951 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 26 KEDEWRMAFDRLMME 15 3324.0954 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 31 RMAFDRLMMEELETK 15 3324.0956 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 51 KGLLHLSEQYKELEK 15 3324.0955 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 86 MKGALKFFEMEAKRT 15 3324.0950 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 106 ERYNYEFALESIKLL 15 3324.0948 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 111 EFALESIKLLIKKLD 15 3324.0952 pteronyssinus Dermatophagoides 6956 Der p 21 Q2L7C5 85687540 116 SIKLLIKKLDELAKK 15 3324.0953 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 1 MIIYNILIVLLLAIN 15 3324.0957 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 6 ILIVLLLAINTLANP 15 3324.0958 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 11 LLAINTLANPILPAS 15 3324.0962 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 16 TLANPILPASPNATI 15 3324.0969 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 41 CPYQISLQSSSHFCG 15 3324.0961 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 56 GTILDEYWILTAAHC 15 3324.0959 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 61 EYWILTAAHCVAGQT 15 3324.0966 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 76 ASKLSIRYNSLKHSL 15 3324.0963 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 81 IRYNSLKHSLGGEKI 15 3324.0968 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 91 GGEKISVAKIFAHEK 15 3324.0964 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 111 IDNDIALIKLKSPMK 15 3324.0967 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 116 ALIKLKSPMKLNQKN 15 3324.0965 pteronyssinus Dermatophagoides 6956 Der p 3 P39675 729315 166 SELRRVDIAVVSRKE 15 3324.0960 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 1 KYHNPHFIGNRSVIT 15 3324.0983 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 6 HFIGNRSVITHLMEW 15 3324.0982 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 86 NKAGVRIYVDIVLNH 15 3324.0981 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 91 RIYVDIVLNHMTGAQ 15 3324.0988 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 96 IVLNHMTGAQSGKGT 15 3324.0987 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 176 QVDFLNHLIDIGVAG 15 3324.0984 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 201 PDDLRSIYSRLHNLN 15 3324.0979 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 206 SIYSRLHNLNKEFFP 15 3324.0989 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 221 ENSQPFIYHETIYYG 15 3324.0990 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 251 IEFRFYKEITNVFRG 15 3324.0976 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 286 DALVMIDSHDLRVGH 15 3324.0992 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 311 FEGRLLKAATAFMLA 15 3324.0973 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 316 LKAATAFMLAWNYGV 15 3324.0972 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 321 AFMLAWNYGVPRVMS 15 3324.0977 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 331 PRVMSSYFWNQIIKD 15 3324.0991 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 381 EHRWREIYNMVKFRM 15 3324.0986 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 386 EIYNMVKFRMIAGQE 15 3324.0978 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 391 VKFRMIAGQEPVHNW 15 3324.0993 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 411 YQIAFSRGNRAFIAI 15 3324.0980 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 416 SRGNRAFIAINLQKN 15 3324.0974 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 421 AFIAINLQKNQQNLQ 15 3324.0985 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 476 YVGHDEFDAFVAYHI 15 3324.0975 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 481 EFDAFVAYHIGARIV 15 3324.0970 pteronyssinus Dermatophagoides 6956 Der p 4 Q9Y197 5059162 482 FDAFVAYHIGARIVS 15 3324.0971 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 6 KDPKPLKKISIMKFI 15 3324.0999 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 11 LKKISIMKFIIAFFV 15 3324.0996 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 16 IMKFIIAFFVATLAV 15 3324.0994 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 21 IAFFVATLAVMTVSG 15 3324.0995 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 46 FDFLLMERIHEQIKK 15 3324.0998 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 61 GELALFYLQEQINHF 15 3324.0997 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 66 FYLQEQINHFEEKPT 15 3324.1000 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 106 DRLMQRKDLDIFEQY 15 3324.1002 pteronyssinus Dermatophagoides 6956 Der p 5 P14004V 1352238 111 RKDLDIFEQYNLEMA 15 3324.1001 pteronyssinus Dermatophagoides 6956 Der p 6 P49277(IUIS) 1352239 1 MIKIITTIILIITVV 15 3324.1003 pteronyssinus Dermatophagoides 6956 Der p 6 P49277(IUIS) 1352239 6 TTIILIITVVVDCRF 15 3324.1004 pteronyssinus Dermatophagoides 6956 Der p 6 P49277(IUIS) 1352239 26 PKWSYLDSLPASSSM 15 3324.1005 pteronyssinus Dermatophagoides 6956 Der p 6 P49277(IUIS) 1352239 36 ASSSMMNDNSSPIAG 15 3337.0027 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 1 MMKLLLIAAAAFVAV 15 3324.1006 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 6 LIAAAAFVAVSADPI 15 3324.1007 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 11 AFVAVSADPIHYDKI 15 3324.1012 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 111 VVSMEYDLAYKLGDL 15 3324.1015 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 131 VISDIQDFVVELSLE 15 3324.1019 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 136 QDFVVELSLEVSEEG 15 3324.1009 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 151 NMTLTSFEVRQFANV 15 3324.1014 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 156 SFEVRQFANVVNHIG 15 3324.1010 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 161 QFANVVNHIGGLSIL 15 3324.1016 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 166 VNHIGGLSILDPIFA 15 3324.1013 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 171 GLSILDPIFAVLSDV 15 3324.1011 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 176 DPIFAVLSDVLTAIF 15 3324.1008 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 186 LTAIFQDTVRAEMTK 15 3324.1018 pteronyssinus Dermatophagoides 6956 Der p 7 P49273 292659601 196 AEMTKVLAPAFKKEL 15 3324.1017 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 16 QPIRLLLTYSGVDFV 15 3324.1025 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 46 EWLNEKFNLGLDFPN 15 3324.1029 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 66 DGDMKMTQTFAILRY 15 3324.1027 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 71 MTQTFAILRYLGRKY 15 3324.1022 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 136 LKLMSKFVGEHAFIA 15 3324.1023 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 141 KFVGEHAFIAGANIS 15 3324.1021 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 146 HAFIAGANISYVDFN 15 3324.1020 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 156 YVDFNLYEYLCHVKV 15 3324.1026 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 171 MVPEVFGQFENLKRY 15 3324.1024 pteronyssinus Dermatophagoides 6956 Der p 8 P46419 1170095 181 NLKRYVERMESLPRV 15 3324.1028 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 1 MKFMILFALIAIGTS 15 3324.1030 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 6 LFALIAIGTSVAIGE 15 3324.1038 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 31 ITEKFPWMINEPLND 15 3324.1034 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 56 ASPGDAVYQIALFRK 15 3324.1041 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 61 AVYQIALFRKDSFTC 15 3324.1044 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 76 GGSLISSRTVLTAAH 15 3324.1042 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 96 EATPSYFKIRYNTLD 15 3324.1031 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 101 YFKIRYNTLDRTNGP 15 3324.1032 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 121 KIYRHNLYSSSPIDY 15 3324.1045 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 131 SPIDYDVATLILSQP 15 3324.1033 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 136 DVATLILSQPFTPSA 15 3324.1037 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 181 TLPTILQIASVTKMS 15 3324.1036 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 201 STWGSVNAITNRMLC 15 3324.1035 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 251 STKYPTIYSNVANLR 15 3324.1039 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 256 TIYSNVANLRNWIIS 15 3324.1040 pteronyssinus Dermatophagoides 6956 Der p 9 Q7Z163 31745576 259 SNVANLRNWIISNTV 15 3324.1043 pteronyssinus Dog epithelia 9615 Can f 1 O18873 29292148 1 MKTLLLTIGFSLIAI 15 3324.0474 Dog epithelia 9615 Can f 1 O18873 29292148 6 LTIGFSLIAILQAQD 15 3324.0473 Dog epithelia 9615 Can f 1 O18873 29292148 11 SLIAILQAQDTPALG 15 3324.0475 Dog epithelia 9615 Can f 1 O18873 29292148 31 VSGKWYLKAMTADQE 15 3324.0478 Dog epithelia 9615 Can f 1 O18873 29292148 36 YLKAMTADQEVPEKP 15 3324.0480 Dog epithelia 9615 Can f 1 O18873 29292148 91 PGKYTAYEGQRVVFI 15 3324.0479 Dog epithelia 9615 Can f 1 O18873 29292148 96 AYEGQRVVFIQPSPV 15 3324.0476 Dog epithelia 9615 Can f 1 O18873 29292148 101 RVVFIQPSPVRDHYI 15 3324.0477 Dog epithelia 9615 Can f 2 O18874 3121746 1 MQLLLLTVGLALICG 15 3324.0482 Dog epithelia 9615 Can f 2 O18874 3121746 36 RWHSVALASNKSDLI 15 3324.0484 Dog epithelia 9615 Can f 2 O18874 3121746 51 KPWGHFRVFIHSMSA 15 3324.0483 Dog epithelia 9615 Can f 2 O18874 3121746 56 FRVFIHSMSAKDGNL 15 3324.0485 Dog epithelia 9615 Can f 2 O18874 3121746 81 QCEKVSLTAFKTATS 15 3324.0486 Dog epithelia 9615 Can f 2 O18874 3121746 86 SLTAFKTATSNKFDL 15 3324.0489 Dog epithelia 9615 Can f 2 O18874 3121746 116 KSYLILYMINQYNDD 15 3324.0481 Dog epithelia 9615 Can f 2 O18874 3121746 121 LYMINQYNDDTSLVA 15 3324.0488 Dog epithelia 9615 Can f 2 O18874 3121746 131 TSLVAHLMVRDLSRQ 15 3324.0487 Dog epithelia 9615 Can f 3 P49822 56757408 1 MKWVTFISLFFLFSS 15 3324.0490 Dog epithelia 9615 Can f 3 P49822 56757408 6 FISLFFLFSSAYSRG 15 3324.0491 Dog epithelia 9615 Can f 3 P49822 56757408 21 LVRREAYKSEIAHRY 15 3324.0511 Dog epithelia 9615 Can f 3 P49822 56757408 41 EHFRGLVLVAFSQYL 15 3324.0493 Dog epithelia 9615 Can f 3 P49822 56757408 46 LVLVAFSQYLQQCPF 15 3324.0496 Dog epithelia 9615 Can f 3 P49822 56757408 156 QLFLGKYLYEIARRH 15 3324.0499 Dog epithelia 9615 Can f 3 P49822 56757408 161 KYLYEIARRHPYFYA 15 3324.0501 Dog epithelia 9615 Can f 3 P49822 56757408 166 IARRHPYFYAPELLY 15 3324.0502 Dog epithelia 9615 Can f 3 P49822 56757408 171 PYFYAPELLYYAQQY 15 3324.0506 Dog epithelia 9615 Can f 3 P49822 56757408 176 PELLYYAQQYKGVFA 15 3324.0508 Dog epithelia 9615 Can f 3 P49822 56757408 206 IEALREKVLLSSAKE 15 3324.0510 Dog epithelia 9615 Can f 3 P49822 56757408 231 GDRAFKAWSVARLSQ 15 3324.0495 Dog epithelia 9615 Can f 3 P49822 56757408 346 AKDVFLGTFLYEYAR 15 3324.0507 Dog epithelia 9615 Can f 3 P49822 56757408 351 LGTFLYEYARRHPEY 15 3324.0504 Dog epithelia 9615 Can f 3 P49822 56757408 361 RHPEYSVSLLLRLAK 15 3324.0497 Dog epithelia 9615 Can f 3 P49822 56757408 366 SVSLLLRLAKEYEAT 15 3324.0505 Dog epithelia 9615 Can f 3 P49822 56757408 396 KVLDEFKPLVDEPQN 15 3324.0503 Dog epithelia 9615 Can f 3 P49822 56757408 421 KLGEYGFQNALLVRY 15 3324.0498 Dog epithelia 9615 Can f 3 P49822 56757408 471 SCAEDFLSVVLNRLC 15 3324.0494 Dog epithelia 9615 Can f 3 P49822 56757408 476 FLSVVLNRLCVLHEK 15 3324.0500 Dog epithelia 9615 Can f 3 P49822 56757408 531 FTFHADLCTLPEAEK 15 3324.0509 Dog epithelia 9615 Can f 3 P49822 56757408 594 EEGPKLVAAAQAALV 15 3324.0492 Dog epithelia 9615 Can f 5 P09582 50979094 1 MWFLALCLAMSLGWT 15 3324.0513 Dog epithelia 9615 Can f 5 P09582 50979094 86 EGQLVQVRKSFIHPL 15 3324.0514 Dog epithelia 9615 Can f 5 P09582 50979094 91 QVRKSFIHPLYKTKV 15 3324.0512 Dog epithelia 9615 Can f 5 P09582 50979094 116 RSHDLMLLHLEEPAK 15 3324.0515 Dog epithelia 9615 Can f 5 P09582 50979094 191 VTKFMLCAGVLEGKK 15 3324.0516 English plantain 39414 Pla l 1 P82242 14422363 21 HSRNLINELSERMAG 15 3324.1552 English plantain 39414 Pla l 1 P82242 14422363 71 HEDCEIKLVKSSRPD 15 3324.1551 English plantain 39414 Pla l 1 P82242 14422363 76 IKLVKSSRPDCSEIP 15 3337.0044 Giant ragweed 4214 Amb t 5 P10414 1184267 1 MKNIFMLTLFILIIT 15 3324.0099 Giant ragweed 4214 Amb t 5 P10414 1184267 6 MLTLFILIITSTIKA 15 3324.0100 Giant ragweed 4214 Amb t 5 P10414 1184267 11 ILIITSTIKAIGSTN 15 3324.0101 Giant ragweed 4214 Amb t 5 P10414 1184267 31 KQEDDGLCYEGTNCG 15 3337.0006 Giant ragweed 4214 Amb t 5 P10414 1184267 56 GKYCVCYDSKAICNK 15 3337.0007 Kentucky blue grass 4545 Poa p 1 Q9ZP03 4090265 1 MASSSSVLLVVALFA 15 3324.1554 Kentucky blue grass 4545 Poa p 1 Q9ZP03 4090265 6 SVLLVVALFAVFLGT 15 3324.1553 Kentucky blue grass 4545 Poa p 1 Q9ZP03 4090265 11 VALFAVFLGTAHGIA 15 3324.1555 Kentucky blue grass 4545 Poa p 1 Q9ZP03 4090265 121 EPIAAYHFDLSGKAF 15 3324.1556 Kentucky blue grass 4545 Poa p 1 Q9ZP03 4090265 176 KGSNPNYLALLVKYV 15 3324.1557 Kentucky blue grass 4545 Poa p 1 Q9ZP03 4090265 181 NYLALLVKYVTGDGD 15 3324.1558 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 1 MAVQKYTVALFLTVA 15 3324.1565 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 6 YTVALFLTVALVAGP 15 3324.1561 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 11 FLTVALVAGPAASYA 15 3324.1572 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 51 QKLMEDINVGFKAAV 15 3324.1580 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 56 DINVGFKAAVAAAAG 15 3324.1571 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 61 FKAAVAAAAGAPPAD 15 3324.1575 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 71 APPADKFKTFQAAFS 15 3324.1568 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 76 KFKTFQAAFSASVEA 15 3324.1560 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 101 PGFVSHVAATSDATY 15 3324.1573 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 121 ATPEAKFDSFVAAFT 15 3324.1570 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 126 KFDSFVAAFTEALRI 15 3324.1559 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 131 VAAFTEALRIIAGVL 15 3324.1576 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 136 EALRIIAGVLKVHAV 15 3324.1566 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 141 IAGVLKVHAVKPITE 15 3324.1581 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 171 DKIDAAFKVAATAAN 15 3324.1569 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 176 AFKVAATAANAAPAN 15 3324.1578 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 191 DKFTVFEAAFNNAIK 15 3324.1562 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 196 FEAAFNNAIKESTGG 15 3324.1574 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 206 ESTGGAYDTYKSIPS 15 3324.1583 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 211 AYDTYKSIPSLEAAV 15 3324.1579 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 221 LEAAVKQAYAATIAA 15 3324.1564 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 226 KQAYAATIAAAPEVK 15 3324.1582 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 236 APEVKFAVFKAALTK 15 3324.1567 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 241 FAVFKAALTKAITAM 15 3324.1563 Kentucky blue grass 4545 Poa p 5 Q9FPR0 11991227 246 AALTKAITAMAEVQK 15 3324.1577 Mugwort 4220 Art v 1 Q84ZX5 27818335 1 MAKCSYVFCAVLLIF 15 3324.0106 Mugwort 4220 Art v 1 Q84ZX5 27818335 6 YVFCAVLLIFIVAIG 15 3324.0104 Mugwort 4220 Art v 1 Q84ZX5 27818335 11 VLLIFIVAIGEMEAA 15 3324.0105 Mugwort 4220 Art v 1 Q84ZX5 27818335 31 EKTSKTYSGKCDNKK 15 3337.0009 Mugwort 4220 Art v 1 Q84ZX5 27818335 71 CFCYFDCSKSPPGAT 15 3337.0010 Mugwort 4220 Art v 2 A6GVD5 148887203 1 MGHLGNFWLVLAISF 15 3324.0109 Mugwort 4220 Art v 2 A6GVD5 148887203 6 NFWLVLAISFAILHL 15 3324.0107 Mugwort 4220 Art v 2 A6GVD5 148887203 11 LAISFAILHLSHAHE 15 3324.0108 Mugwort 4220 Art v 2 A6GVD5 148887203 86 AQGAINGSMAVQLWL 15 3324.0111 Mugwort 4220 Art v 2 A6GVD5 148887203 121 TQIVWANSERVGCGR 15 3337.0011 Mugwort 4220 Art v 2 A6GVD5 148887203 141 GWAYIIVCNYDPPGN 15 3324.0110 Mugwort 4220 Art v 3 C4MGH1 189544590 1 MAIKMMKVFCIMVVC 15 3324.0112 Mugwort 4220 Art v 3 C4MGH1 189544590 6 MKVFCIMVVCMVVST 15 3324.0116 Mugwort 4220 Art v 3 C4MGH1 189544590 11 IMVVCMVVSTSYAES 15 3324.0114 Mugwort 4220 Art v 3 C4MGH1 189544590 16 MVVSTSYAESALTCS 15 3324.0117 Mugwort 4220 Art v 3 C4MGH1 189544590 76 CLKASFKSNKDLKSD 15 3337.0012 Mugwort 4220 Art v 3 C4MGH1 189544590 81 FKSNKDLKSDFAVPL 15 3324.0115 Mugwort 4220 Art v 3 C4MGH1 189544590 86 DLKSDFAVPLPSKCG 15 3324.0113 Mugwort 4220 Art v 4 Q8H2C8 73621416 16 EGTGQHLTAAAILGL 15 3324.0119 Mugwort 4220 Art v 4 Q8H2C8 73621416 51 GIINEFNEVGTLAPT 15 3337.0013 Mugwort 4220 Art v 4 Q8H2C8 73621416 66 GLFLGGAKYMVLQGE 15 3324.0120 Mugwort 4220 Art v 4 Q8H2C8 73621416 71 GAKYMVLQGEAGAVI 15 3324.0118 Orchard grass 4509 Dac g 1 Q7XAX7 18093991 6 SSVLLVVALFAVFLG 15 3324.0653 Orchard grass 4509 Dac g 1 Q7XAX7 18093991 11 VVALFAVFLGSAHGI 15 3324.0654 Orchard grass 4509 Dac g 1 Q7XAX7 18093991 121 EEPIAPYHFDLSGHA 15 3324.0656 Orchard grass 4509 Dac g 1 Q7XAX7 18093991 181 PNYLALLVKYVDGDG 15 3324.0655 Orchard grass 4509 Dac g 1 Q7XAX7 18093991 211 IALKESWGAIWRVDT 15 3337.0022 Orchard grass 4509 Dac g 2 Q41183 255657 11 GSDEKNLALSIKYNK 15 3324.0657 Orchard grass 4509 Dac g 2 Q41183 255657 16 NLALSIKYNKEGDSM 15 3324.0658 Orchard grass 4509 Dac g 2 Q41183 255657 21 IKYNKEGDSMAEVEL 15 3337.0023 Orchard grass 4509 Dac g 3 P93124 14423759 51 LWEVKSSKPLTGPFN 15 3337.0024 Orchard grass 4509 Dac g 3 P93124 14423759 76 NVFDEVIPTAFKIGT 15 3324.0659 Orchard grass 4509 Dac g 3 P93124 14423759 82 IPTAFKIGTTYTPEE 15 3324.0660 Orchard grass 4509 Dac g 4 P82946 32363463 1 DIYNYMEPYVSKVDP 15 3324.0663 Orchard grass 4509 Dac g 4 P82946 32363463 26 TAWVDSGAQLGELSY 15 3337.0025 Orchard grass 4509 Dac g 4 P82946 32363463 36 GELSYGVLFNIQYVN 15 3324.0662 Orchard grass 4509 Dac g 4 P82946 32363463 41 GVLFNIQYVNYWFAP 15 3324.0661 Penicillium 5076 Pen ch 13 Q9URR2 6684758 1 MGFLKVLATSLATLA 15 3324.1295 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 6 VLATSLATLAVVDAG 15 3324.1300 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 26 SNTDAVIPSSYIVVM 15 3324.1309 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 31 VIPSSYIVVMNDDVS 15 3324.1310 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 36 YIVVMNDDVSTAEFS 15 3324.1311 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 101 PAVKYIEPDMIVNAT 15 3324.1304 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 106 IEPDMIVNATANVVQ 15 3324.1298 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 111 IVNATANVVQSNVPS 15 3324.1306 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 126 WGLARISSKRTGTTS 15 3324.1312 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 201 YGVAKKATLVAVKVL 15 3324.1305 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 206 KATLVAVKVLGADGS 15 3324.1307 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 261 NDAAANVVKSGIFLS 15 3324.1308 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 266 NVVKSGIFLSVAAGN 15 3324.1299 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 271 GIFLSVAAGNEAENA 15 3324.1303 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 346 APHVAGVAAYLMALE 15 3324.1296 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 351 GVAAYLMALEGVSAG 15 3324.1297 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 366 NACARIVQLATSSIS 15 3324.1301 chrysogenum Penicillium 5076 Pen ch 13 Q9URR2 6684758 371 IVQLATSSISRAPSG 15 3324.1302 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 1 MKGFLSLTLLPLLVA 15 3324.1314 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 6 SLTLLPLLVAASPVA 15 3324.1315 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 11 PLLVAASPVAVNSIH 15 3324.1319 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 26 NDAAPILSSMTSKDI 15 3324.1332 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 81 SLFGFDFEAFMGLKH 15 3324.1316 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 91 MGLKHTFHIAGSLLG 15 3324.1318 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 96 TFHIAGSLLGYAGHF 15 3324.1323 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 176 VDAYVIDTGANVKHV 15 3324.1327 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 226 KFGVAKKANVYAVKV 15 3324.1330 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 236 YAVKVLRSNGSGTMS 15 3324.1320 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 271 DKKFKGSVANMSLGG 15 3324.1329 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 291 LDLAVNAAVDAGIHF 15 3324.1331 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 301 AGIHFAVAAGNDNAD 15 3324.1324 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 351 IFAPGLNILSTWVGS 15 3324.1325 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 376 SMASPHIAGLLAYYV 15 3324.1321 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 381 HIAGLLAYYVSLAPA 15 3324.1313 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 406 TPKQLKAALISVATE 15 3324.1317 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 411 KAALISVATEGTLTD 15 3324.1326 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 466 IGIIIDSAEKAFHKE 15 3324.1322 chrysogenum Penicillium 5076 Pen ch 18 Q9P8G3 7963902 476 AFHKELGAIYSEIKD 15 3324.1328 chrysogenum Prickly juniper 69008 Jun o 4 O64943 5391446 46 ELADILRSLGSDVGE 15 3324.1129 Prickly juniper 69008 Jun o 4 O64943 5391446 76 GYVSLQEFVDLNNKG 15 3324.1131 Prickly juniper 69008 Jun o 4 O64943 5391446 146 LISVEEFQTMMTSEM 15 3324.1130 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 81 EKVKIERLHPYITLY 15 3324.1599 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 86 ERLHPYITLYGIDPK 15 3324.1597 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 101 NRPTITFAGTAAEFG 15 3324.1595 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 111 AAEFGTVDSATLIVE 15 3324.1596 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 126 SDYFVGANLIVSNSA 15 3324.1594 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 131 GANLIVSNSAPRPDG 15 3324.1601 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 191 EGTVDFIFGEARSLY 15 3324.1590 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 196 FIFGEARSLYLNTEL 15 3324.1591 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 251 LGRAWFEAARVVFSY 15 3324.1592 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 256 FEAARVVFSYCNLSD 15 3324.1593 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 316 ADAKTFTSLEYIEAA 15 3324.1598 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 321 FTSLEYIEAAKWLLP 15 3324.1589 Russian thistle 151250 Sal k 1 1 Q17ST3 59895730 325 EYIEAAKWLLPPPKV 15 3324.1600 Russian thistle 151250 Sal k 1 2 P83181 25090947 11 RTIFFDAYLGTSYVI 15 3324.1602 Russian thistle 151250 Sal k 1 2 P83181 25090947 16 DAYLGTSYVIVIKEP 15 3324.1603 Russian thistle 151250 Sal k 1 2 P83181 25090947 21 TSYVIVIKEPAEEFT 15 3324.1604 Russian thistle 151250 Sal k 1_2 P83181 25090947 28 KEPAEEFTTISDAVK 15 3337.0045 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 56 ISYRVYTLKEIEVGT 15 3324.1617 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 66 IEVGTDYFSSSLKIG 15 3324.1616 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 86 PVYRAMLQHTPVAIK 15 3324.1610 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 96 PVAIKVLRPNVSQGL 15 3324.1615 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 141 LVYEYMENGSLEDRL 15 3324.1614 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 151 LEDRLFRKNNSPPIP 15 3324.1620 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 166 WKLRFKIAAEIAIAL 15 3324.1607 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 171 KIAAEIAIALLFLRD 15 3324.1606 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 176 IAIALLFLRDAKPEP 15 3324.1608 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 201 ILLDGNYISKIADVG 15 3324.1612 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 211 IADVGLARLVPPTVA 15 3324.1619 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 256 KSDIYSFGIILLQLL 15 3324.1605 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 261 SFGIILLQLLTARPP 15 3324.1609 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 266 LLQLLTARPPMALSY 15 3324.1613 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 301 DWPVQEALSLAQLAL 15 3324.1611 Russian thistle 151250 Sal k 2 Q8L5K9 22726221 306 EALSLAQLALKCCEG 15 3324.1618 Russian thistle 151250 Sal k 3 C1KEU0 225810599 51 DAGIKYIPSNTFAYY 15 3324.1622 Russian thistle 151250 Sal k 3 C1KEU0 225810599 56 YIPSNTFAYYDQVLD 15 3324.1667 Russian thistle 151250 Sal k 3 C1KEU0 225810599 86 GGEIGFDLYFSMARG 15 3324.1642 Russian thistle 151250 Sal k 3 C1KEU0 225810599 91 FDLYFSMARGNASLP 15 3324.1653 Russian thistle 151250 Sal k 3 C1KEU0 225810599 111 KWFDTNYHYIVPELG 15 3324.1654 Russian thistle 151250 Sal k 3 C1KEU0 225810599 126 PEVKFAYSSHKAVDE 15 3324.1644 Russian thistle 151250 Sal k 3 C1KEU0 225810599 136 KAVDEYKEAKALGVD 15 3324.1662 Russian thistle 151250 Sal k 3 C1KEU0 225810599 156 VGPVSYLLLSKAAKG 15 3324.1651 Russian thistle 151250 Sal k 3 C1KEU0 225810599 161 YLLLSKAAKGVEKSF 15 3324.1659 Russian thistle 151250 Sal k 3 C1KEU0 225810599 166 KAAKGVEKSFPLLSL 15 3324.1643 Russian thistle 151250 Sal k 3 C1KEU0 225810599 171 VEKSFPLLSLLPKIL 15 3324.1631 Russian thistle 151250 Sal k 3 C1KEU0 225810599 176 PLLSLLPKILPVYKE 15 3324.1645 Russian thistle 151250 Sal k 3 C1KEU0 225810599 186 PVYKEVIAELKAAGA 15 3324.1634 Russian thistle 151250 Sal k 3 C1KEU0 225810599 191 VIAELKAAGASTIQF 15 3324.1632 Russian thistle 151250 Sal k 3 C1KEU0 225810599 231 STLSGLNVLVETYFA 15 3324.1664 Russian thistle 151250 Sal k 3 C1KEU0 225810599 236 LNVLVETYFADLTPE 15 3324.1655 Russian thistle 151250 Sal k 3 C1KEU0 225810599 241 ETYFADLTPEAYKTL 15 3324.1641 Russian thistle 151250 Sal k 3 C1KEU0 225810599 251 AYKTLVSLNGVTAFG 15 3324.1652 Russian thistle 151250 Sal k 3 C1KEU0 225810599 261 VTAFGFDLVRGTKTL 15 3324.1640 Russian thistle 151250 Sal k 3 C1KEU0 225810599 281 GFPSGKYLFAGVVDG 15 3324.1648 Russian thistle 151250 Sal k 3 C1KEU0 225810599 296 RNIWANDLAASLATL 15 3324.1638 Russian thistle 151250 Sal k 3 C1KEU0 225810599 301 NDLAASLATLQSLES 15 3324.1628 Russian thistle 151250 Sal k 3 C1KEU0 225810599 306 SLATLQSLESIVGKD 15 3324.1647 Russian thistle 151250 Sal k 3 C1KEU0 225810599 341 TKLDDEIKSWLAFAA 15 3324.1630 Russian thistle 151250 Sal k 3 C1KEU0 225810599 346 EIKSWLAFAAQKVLE 15 3324.1621 Russian thistle 151250 Sal k 3 C1KEU0 225810599 351 LAFAAQKVLEVNALA 15 3324.1623 Russian thistle 151250 Sal k 3 C1KEU0 225810599 356 QKVLEVNALAKALAG 15 3324.1657 Russian thistle 151250 Sal k 3 C1KEU0 225810599 371 QKDEAFFSANAAALA 15 3324.1624 Russian thistle 151250 Sal k 3 C1KEU0 225810599 376 FFSANAAALASRKSS 15 3324.1636 Russian thistle 151250 Sal k 3 C1KEU0 225810599 461 EEYVKAIKEEISKVV 15 3324.1627 Russian thistle 151250 Sal k 3 C1KEU0 225810599 471 ISKVVKLQEELDIDV 15 3324.1661 Russian thistle 151250 Sal k 3 C1KEU0 225810599 496 MVEYFGEQLSGFAFT 15 3324.1639 Russian thistle 151250 Sal k 3 C1KEU0 225810599 536 KAMTVFWSSLAQSMT 15 3324.1633 Russian thistle 151250 Sal k 3 C1KEU0 225810599 551 SRPMKGMLTGPVTIL 15 3324.1663 Russian thistle 151250 Sal k 3 C1KEU0 225810599 561 PVTILNWSFVRNDQP 15 3324.1646 Russian thistle 151250 Sal k 3 C1KEU0 225810599 576 RHETCYQIALAIEDE 15 3324.1629 Russian thistle 151250 Sal k 3 C1KEU0 225810599 596 KAGINVIQIDEAALR 15 3324.1650 Russian thistle 151250 Sal k 3 C1KEU0 225810599 601 VIQIDEAALREGLPL 15 3324.1665 Russian thistle 151250 Sal k 3 C1KEU0 225810599 621 GFYLQWAVHSFRITN 15 3324.1625 Russian thistle 151250 Sal k 3 C1KEU0 225810599 626 WAVHSFRITNVGIQD 15 3324.1635 Russian thistle 151250 Sal k 3 C1KEU0 225810599 651 SNFNDIIHSIIDMDA 15 3324.1658 Russian thistle 151250 Sal k 3 C1KEU0 225810599 661 IDMDADVITIENSRS 15 3324.1649 Russian thistle 151250 Sal k 3 C1KEU0 225810599 666 DVITIENSRSDEKLL 15 3324.1660 Russian thistle 151250 Sal k 3 C1KEU0 225810599 711 LADRIRKMLAVLESN 15 3324.1637 Russian thistle 151250 Sal k 3 C1KEU0 225810599 716 RKMLAVLESNVLWVN 15 3324.1626 Russian thistle 151250 Sal k 3 C1KEU0 225810599 741 NEVNPALSNMVYAAK 15 3324.1656 Russian thistle 151250 Sal k 3 C1KEU0 225810599 743 VNPALSNMVYAAKPI 15 3324.1666 Russian thistle 151250 Sal k 4 C6JWH0 239916566 16 EGTNNHLTAAAILGV 15 3324.1669 Russian thistle 151250 Sal k 4 C6JWH0 239916566 71 GTKYMVIQGEAGQVI 15 3324.1668 Russian thistle 151250 Sal k 4 C6JWH0 239916566 101 QALIFGIYDEPVTPG 15 3337.0046 Rye grass 4522 Lol p 1 P14946 126385 1 MASSSSVLLVVALFA 15 3324.1133 Rye grass 4522 Lol p 1 P14946 126385 6 SVLLVVALFAVFLGS 15 3324.1132 Rye grass 4522 Lol p 1 P14946 126385 11 VALFAVFLGSAHGIA 15 3324.1134 Rye grass 4522 Lol p 1 P14946 126385 121 EPIAPYHFDLSGHAF 15 3337.0028 Rye grass 4522 Lol p 1 P14946 126385 176 KASNPNYLAILVKYV 15 3324.1135 Rye grass 4522 Lol p 1 P14946 126385 181 NYLAILVKYVDGDGD 15 3324.1136 Rye grass 4522 Lol p 11 Q7M1X5 47605808 106 KQQGIRYANPIAFFR 15 3324.1137 Rye grass 4522 Lol p 11 Q7M1X5 47605808 111 RYANPIAFFRKEPLK 15 3324.1138 Rye grass 4522 Lol p 11 Q7M1X5 47605808 116 IAFFRKEPLKECGGI 15 3337.0029 Rye grass 4522 Lol p 2 P14947 126386 51 DGVWEIKSDKPLKGP 15 3324.1139 Rye grass 4522 Lol p 2 P14947 126386 56 IKSDKPLKGPFNFRF 15 3324.1140 Rye grass 4522 Lol p 2 P14947 126386 76 MRNVFDDVVPADFKV 15 3337.0030 Rye grass 4522 Lol p 3 P14948 126387 11 SDAKTLVLNIKYTRP 15 3324.1142 Rye grass 4522 Lol p 3 P14948 126387 51 LWEVKSAKPLTGPMN 15 3337.0031 Rye grass 4522 Lol p 3 P14948 126387 76 NVFDEVIPTAFTVGK 15 3324.1141 Rye grass 4522 Lol p 4 Q5TIW3 55859464 21 YEGLSYRSLQPENFA 15 3324.1161 Rye grass 4522 Lol p 4 Q5TIW3 55859464 31 PENFAVVDLNQMRAV 15 3324.1159 Rye grass 4522 Lol p 4 Q5TIW3 55859464 36 VVDLNQMRAVLVDGK 15 3324.1160 Rye grass 4522 Lol p 4 Q5TIW3 55859464 61 QLGELYYAISKYSRT 15 3324.1146 Rye grass 4522 Lol p 4 Q5TIW3 55859464 66 YYAISKYSRTLAFPA 15 3324.1147 Rye grass 4522 Lol p 4 Q5TIW3 55859464 71 KYSRTLAFPAGVCPT 15 3324.1164 Rye grass 4522 Lol p 4 Q5TIW3 55859464 96 GFGMLLRKYGIAAEN 15 3324.1157 Rye grass 4522 Lol p 4 Q5TIW3 55859464 101 LRKYGIAAENVIDVK 15 3324.1145 Rye grass 4522 Lol p 4 Q5TIW3 55859464 141 GGESFGIVVSWQVKL 15 3324.1153 Rye grass 4522 Lol p 4 Q5TIW3 55859464 146 GIVVSWQVKLLPVPP 15 3324.1156 Rye grass 4522 Lol p 4 Q5TIW3 55859464 176 VDIINKWQLVAPQLP 15 3324.1143 Rye grass 4522 Lol p 4 Q5TIW3 55859464 191 ADLMIRIIAMGPKAT 15 3324.1155 Rye grass 4522 Lol p 4 Q5TIW3 55859464 236 NEMSWIESIPFVHLG 15 3324.1151 Rye grass 4522 Lol p 4 Q5TIW3 55859464 261 NRNNTFKPFAEYKSD 15 3324.1158 Rye grass 4522 Lol p 4 Q5TIW3 55859464 266 FKPFAEYKSDYVYEP 15 3324.1148 Rye grass 4522 Lol p 4 Q5TIW3 55859464 271 EYKSDYVYEPFPKSV 15 3324.1162 Rye grass 4522 Lol p 4 Q5TIW3 55859464 301 IMIFDPYGATISATP 15 3324.1149 Rye grass 4522 Lol p 4 Q5TIW3 55859464 321 FPHRKGVLFNIQYVN 15 3324.1152 Rye grass 4522 Lol p 4 Q5TIW3 55859464 326 GVLFNIQYVNYWFAP 15 3324.1144 Rye grass 4522 Lol p 4 Q5TIW3 55859464 331 IQYVNYWFAPGAGAA 15 3324.1150 Rye grass 4522 Lol p 4 Q5TIW3 55859464 351 KEIYNYMEPYVSKNP 15 3324.1154 Rye grass 4522 Lol p 4 Q5TIW3 55859464 396 GQKYFKGNFERLAIT 15 3324.1163 Rye grass 4522 Lol p 5 1 Q40237 2498582 1 MAVQKHTVALFLAVA 15 3324.1172 Rye grass 4522 Lol p 5 1 Q40237 2498582 6 HTVALFLAVALVAGP 15 3324.1166 Rye grass 4522 Lol p 5 1 Q40237 2498582 11 FLAVALVAGPAASYA 15 3324.1170 Rye grass 4522 Lol p 5 1 Q40237 2498582 71 LIEKINAGFKAAVAA 15 3324.1175 Rye grass 4522 Lol p 5 1 Q40237 2498582 76 NAGFKAAVAAAAVVP 15 3324.1168 Rye grass 4522 Lol p 5 1 Q40237 2498582 96 KTFVETFGTATNKAF 15 3324.1171 Rye grass 4522 Lol p 5 1 Q40237 2498582 106 TNKAFVEGLASGYAD 15 3324.1186 Rye grass 4522 Lol p 5 1 Q40237 2498582 126 LTSKLDAALKLAYEA 15 3324.1187 Rye grass 4522 Lol p 5 1 Q40237 2498582 131 DAALKLAYEAAQGAT 15 3324.1169 Rye grass 4522 Lol p 5 1 Q40237 2498582 146 PEAKYDAYVATLTEA 15 3324.1180 Rye grass 4522 Lol p 5 1 Q40237 2498582 151 DAYVATLTEALRVIA 15 3324.1167 Rye grass 4522 Lol p 5 1 Q40237 2498582 156 TLTEALRVIAGTLEV 15 3324.1185 Rye grass 4522 Lol p 5 1 Q40237 2498582 196 VDAAYRTAATAANAA 15 3324.1181 Rye grass 4522 Lol p 5 1 Q40237 2498582 216 FTVFENTFNNAIKVS 15 3324.1179 Rye grass 4522 Lol p 5 1 Q40237 2498582 221 NTFNNAIKVSLGAAY 15 3324.1176 Rye grass 4522 Lol p 5 1 Q40237 2498582 226 AIKVSLGAAYDSYKF 15 3324.1173 Rye grass 4522 Lol p 5 1 Q40237 2498582 231 LGAAYDSYKFIPTLV 15 3324.1174 Rye grass 4522 Lol p 5 1 Q40237 2498582 236 DSYKFIPTLVAAVKQ 15 3324.1165 Rye grass 4522 Lol p 5 1 Q40237 2498582 241 IPTLVAAVKQAYAAK 15 3324.1182 Rye grass 4522 Lol p 5 1 Q40237 2498582 246 AAVKQAYAAKQATAP 15 3324.1177 Rye grass 4522 Lol p 5 1 Q40237 2498582 261 EVKYTVSETALKKAV 15 3324.1178 Rye grass 4522 Lol p 5 1 Q40237 2498582 271 LKKAVTAMSEAEKEA 15 3324.1183 Rye grass 4522 Lol p 5 1 Q40237 2498582 306 PAAAYATATPAAATA 15 3324.1184 Rye grass 4522 Lol p 5 2 Q40240 485371 1 MAVQKYTVALFLRRG 15 3324.1195 Rye grass 4522 Lol p 5 2 Q40240 485371 141 RSLRVIAGALEVHAV 15 3324.1190 Rye grass 4522 Lol p 5 2 Q40240 485371 176 DKIDAAFKIAATAAN 15 3324.1192 Rye grass 4522 Lol p 5 2 Q40240 485371 181 AFKIAATAANAAPTN 15 3324.1197 Rye grass 4522 Lol p 5 2 Q40240 485371 196 DKFTVFESAFNKALN 15 3324.1191 Rye grass 4522 Lol p 5 2 Q40240 485371 241 APEVKYAVFEAALTK 15 3324.1188 Rye grass 4522 Lol p 5 2 Q40240 485371 246 YAVFEAALTKAITAM 15 3324.1194 Rye grass 4522 Lol p 5 2 Q40240 485371 251 AALTKAITAMTQAQK 15 3324.1196 Rye grass 4522 Lol p 5 2 Q40240 485371 291 LPPPLLVVQSLISLL 15 3324.1193 Rye grass 4522 Lol p 5 2 Q40240 485371 294 PLLVVQSLISLLIYY 15 3324.1189 Sweet vernal grass 29661 Ant o 1 Q7M1X6 75139986 1 IAKVPPGPNITATYG 15 3324.0103 Sweet vernal grass 29661 Ant o 1 Q7M1X6 75139986 16 DKWLDAKSTWYGKPT 15 3324.0102 Sweet vernal grass 29661 Ant o 1 Q7M1X6 75139986 18 WLDAKSTWYGKPTGA 15 3337.0008 Western ragweed 29715 Amb p 5 P43174 1171001 1 MNNEKNVSFEFIGST 15 3324.0097 Western ragweed 29715 Amb p 5 P43174 1171001 6 NVSFEFIGSTDEVDE 15 3324.0098 Western ragweed 29715 Amb p 5 P43174 1171001 21 IKLLPCAWAGNVCGE 15 3337.0004 Western ragweed 29715 Amb p 5 P43174 1171001 63 QKCGKMRMNVTKNTI 15 3337.0005 White oak 3513 Que a 1 B6RQS2 167472849 11 ASVIPPARLFKAFVL 15 3324.1587 White oak 3513 Que a 1 B6RQS2 167472849 16 PARLFKAFVLDSDNL 15 3324.1585 White oak 3513 Que a 1 B6RQS2 167472849 21 KAFVLDSDNLIPKVV 15 3324.1588 White oak 3513 Que a 1 B6RQS2 167472849 141 ASEVFKAVEAYLVAH 15 3324.1584 White oak 3513 Que a 1 B6RQS2 167472849 146 KAVEAYLVAHPDLYK 15 3324.1586

TABLE 6 Summary of responses to allergen extracts Concentration Wheal Response to extract (SFC) Summary Allergen (μg/ml) Donor (mm) IFNg IL-5 Total Evaluation Tested Positive % Positive Alder 50 D00069 12 3407 667 4073 + 12 11 91.7 U00110 10 220 3 223 + U00122 10 597 1860 2457 + Alder U00047 10 70 297 367 + Alternaria U00046 10 1323 197 1520 + American cockroach U00050 10 987 100 1087 + Ash U00054 10 1123 830 1953 + Aspergillus fumigatus U00064 10 200 30 230 + Bermuda U00075 3 227 10 237 + Birch U00039 10 33 0 33 − Black walnut U00032 10 997 198 1194 + Canary grass U00115 10 1010 0 1010 + Cat epithelia Alternaria rot 2 D00069 15 33 2460 2493 + 12 12 100.0 Cladosporium fungus U00119 3 953 3370 4323 + Cypress U00122 10 2573 1310 3883 + D. farinae U00064 7 677 2280 2957 + D. pteronyssinus U00102 7 1197 1580 2777 + Dog epithelia D00095 9 1157 2400 3557 + English plantain U00032 8 173 203 377 + German cockroach U00129 3 207 3183 3390 + Giant ragweed D00003 8 1760 2913 4673 + Juniper U00075 8 540 2460 3000 + Kentucky blue U00097 5 2827 593 3420 + Mugwort D00107 14 43 313 357 + Oak Ash 50 D00069 15 170 0 170 + 14 11 78.6 Olive U00089 12 600 310 910 + Orchard D00052 9 567 143 710 + Palm U00058 3 0 0 0 − Penicillium U00109 20 1860 1180 3040 + Russian thistle U00122 12 1473 1180 2653 + Rye U00047 10 210 887 1097 + Sweet vernal grass U00046 10 170 0 170 + Timothy U00102 3 7 3 10 − Western ragweed D00026 25 2177 480 2657 + Wheat U00115 10 107 40 147 − D00007 9 567 33 600 + D00047 20 1833 2313 4147 + D00027 12 763 0 763 + A. fumigatus 5 U00109 3 2853 217 3070 + 11 11 100.0 U00122 3 2610 2250 4860 + D00045 8 1383 47 1430 + U00047 3 593 30 623 + U00050 3 1997 530 2527 + U00129 3 720 600 1320 + U00075 3 1407 57 1463 + U00085 3 1260 57 1317 + U00103 3 1250 63 1313 + U00113 3 3393 10 3403 + U00121 3 1500 353 1853 + American 25 U00109 3 2853 217 3070 + 11 11 100.0 cockroach U00122 3 2610 2250 4860 + D00045 8 1383 47 1430 + U00047 3 593 30 623 + U00050 3 1997 530 2527 + U00129 3 720 600 1320 + U00075 3 1407 57 1463 + U00085 3 1260 57 1317 + U00103 3 1250 63 1313 + U00113 3 3393 10 3403 + U00121 3 1500 353 1853 + Bermuda grass 50 D00070 11 2010 97 2107 + 11 11 100.0 U00089 15 2450 510 2960 + D00004 20 1833 547 2380 + D00039 17 2020 507 2527 + D00094 20 33 2100 2133 + D00102 13 213 1073 1287 + D00067 14 1580 23 1603 + U00054 10 1457 0 1457 + U00099 10 1380 603 1983 + D00042 17 973 23 997 + D00104 11 1620 30 1650 + Birch 50 U00110 20 307 207 513 + 10 10 100.0 U00047 10 380 1840 2220 + D00026 25 2603 407 3010 + U00115 10 617 87 703 + U00103 7 2547 1190 3737 + D00073 19 680 33 713 + D00067 16 2690 387 3077 + D00065 19 1083 97 1180 + D00094 15 350 2710 3060 + U00053 10 1580 327 1907 + Black walnut 50 U00110 10 1410 1910 3320 + 10 10 100.0 U00080 5 2233 2193 4427 + D00094 17 793 1557 2350 + U00115 5 1810 2183 3993 + D00003 6 903 843 1747 + U00075 8 163 2177 2340 + U00092 5 2110 967 3077 + U00097 6 2670 0 2670 + U00072 0 1463 1463 + U00137 4 1330 2197 3527 + Canary grass 50 D00070 21 2770 1183 3953 + 10 10 100.0 U00089 10 1813 910 2723 + U00110 20 163 67 230 + U00099 20 2267 340 2607 + U00106 18 677 1297 1973 + D00004 35 1253 2520 3773 + U00117 7 1573 53 1627 + D00052 17 1670 1803 3473 + U00036 20 787 487 1273 + U00029 20 421 0 421 + Cat epithelia 50 D00069 10 2037 2353 4390 + 11 11 100.0 U00106 12 653 2063 2717 + D00004 8 785 1005 1790 + U00079 8 1177 2100 3277 + D00073 15 700 1113 1813 + U00047 15 307 1933 2240 + U00046 10 737 2620 3357 + U00029 25 1380 1363 2743 + U00128 10 2220 2073 4293 + U00126 8 440 320 760 + U00107 10 820 407 1227 + C. herbarum 5 U00079 3 1703 3917 5620 + 10 10 100.0 U00110 3 640 2040 2680 + U00122 4 2060 2640 4700 + D00073 16 1297 990 2287 + U00083 8 2270 53 2323 + U00102 16 2207 387 2593 + U00101 3 1760 1963 3723 + U00128 3 2933 0 2933 + U00129 3 1397 1287 2683 + U00107 3 1213 263 1477 + Cypress 50 D00052 5 977 797 1773 + 10 8 80.0 U00110 3 97 0 97 − D00026 20 2207 1653 3860 + D00102 12 250 1283 1533 + U00092 3 937 240 1177 + D00008 9 30 2297 2327 + D00107 10 1223 1473 2697 + U00075 3 230 1340 1570 + U00125 3 43 10 53 − U00034 3 2827 247 3073 + D. farinae 50 D00069 16 1303 30 1333 + 10 9 90.0 U00099 10 767 270 1037 + U00117 5 823 237 1060 + U00110 10 207 0 207 + U00093 8 817 217 1033 + U00112 8 343 0 343 + U00131 10 553 0 553 + U00102 3 130 10 140 − U00050 10 540 240 780 + U00124 8 907 1640 2547 + D. 50 U00117 6 2397 2883 5280 + 11 11 100.0 pteronyssinus U00093 10 1167 0 1167 + U00112 8 217 0 217 + U00131 8 1283 303 1587 + D00002 15 663 137 800 + U00050 12 1580 357 1937 + U00102 5 267 40 307 + U00128 20 163 330 493 + U00023 10 860 2173 3033 + U00124 8 2323 2100 4423 + U00108 15 1343 1917 3260 + Dog epithelia 50 U00079 3 283 47 330 + 10 10 100.0 U00117 3 1843 223 2067 + U00110 3 190 33 223 + U00108 3 1150 400 1550 + U00023 4 1460 817 2277 + U00069 5 453 113 567 + U00101 3 903 703 1607 + U00129 3 2110 0 2110 + D00107 10 297 1447 1743 + U00075 3 1453 390 1843 + English plantain 50 D00069 10 2547 2513 5060 + 10 8 80.0 D00004 9 1650 1230 2880 + U00110 8 60 13 73 − D00073 21 240 20 260 + D00094 19 1167 1650 2817 + U00054 10 987 743 1730 + U00075 3 657 530 1187 + U00069 5 83 50 133 − U00102 8 380 243 623 + U00072 5 1467 733 2200 + Giant ragweed 50 D00002 13 1760 120 1880 + 10 10 100.0 D00094 20 173 793 967 + U00047 10 647 1297 1943 + D00095 12 1407 160 1567 + D00007 8 1160 43 1203 + D00100 10 1060 457 1517 + D00045 14 1870 130 2000 + D00084 10 843 1050 1893 + D00096 13 287 0 287 + U00054 8 687 17 703 + Juniper 20 D00052 8 37 0 37 − 10 8 80.0 D00060 9 1530 2413 3943 + U00032 10 153 123 277 + D00073 21 0 0 0 − U00099 8 190 2147 2337 + D00027 6 153 687 840 + D00068 15 540 2717 3257 + U00034 3 637 1450 2087 + D00084 13 183 2227 2410 + U00098 3 2023 330 2353 + Kentucky blue 50 D00070 27 1543 683 2227 + 12 12 100.0 grass U00089 12 2750 2060 4810 + U00106 10 2215 2927 5142 + D00004 22 2417 1813 4230 + U00036 10 1553 2097 3650 + U00102 20 773 2290 3063 + U00032 25 1730 1813 3543 + D00054 10 3133 2353 5487 + U00115 8 1490 713 2203 + U00101 8 1083 2297 3380 + U00110 20 800 543 1343 + U00034 10 423 1837 2260 + Mugwort 50 U00122 12 850 523 1373 + 10 10 100.0 D00061 17 2283 1503 3787 + D00095 8 843 423 1267 + D00007 17 1887 1277 3163 + D00041 12 2670 1603 4273 + D00042 11 933 230 1163 + D00047 16 1213 1273 2487 + U00103 8 533 53 587 + U00118 6 2083 1410 3493 + D00104 17 160 40 200 + White Oak 25 D00069 13 483 1873 2357 + 12 8 66.7 D00004 13 540 587 1127 + D00052 11 110 10 120 − D00026 21 760 340 1100 + D00078 15 153 320 473 + U00047 10 0 2933 2933 + U00103 8 40 0 40 − U00115 8 23 0 23 − D00094 19 23 3583 3607 + U00110 8 77 47 123 − U00072 5 1113 1390 2503 + D00067 11 1273 1183 2457 + Orchard grass 50 D00070 29 2107 350 2457 + 11 11 100.0 U00089 5 2570 2253 4823 + U00110 20 357 930 1287 + U00099 10 37 2433 2470 + U00106 12 510 463 973 + D00004 25 1690 2263 3953 + U00036 10 1310 280 1590 + U00058 8 760 687 1447 + D00042 18 1260 1160 2420 + U00102 20 957 1543 2500 + U00101 12 1050 1020 2070 + Date Palm 50 D00069 9 1927 2517 4443 + 10 9 90.0 U00075 3 873 1347 2220 + U00046 10 2437 1597 4033 + U00050 10 1040 1863 2903 + U00054 10 1010 940 1950 + D00045 24 2223 110 2333 + U00110 10 43 20 63 U00036 8 1127 23 1150 + D00035 5 2223 100 2323 + D00084 13 1963 33 1997 + P. chrysogenum 5 D00052 11 570 890 1460 + 10 10 100.0 U00036 3 2363 237 2600 + U00109 3 837 633 1470 + U00122 3 3870 2423 6293 + U00050 3 460 973 1433 + U00047 3 1570 263 1833 + U00118 6 1710 1363 3073 + U00129 3 2183 1330 3513 + U00132 3 2167 837 3003 + U00137 3 1587 377 1963 + Russian thistle 50 U00089 12 1610 1750 3360 + 10 9 90.0 D00060 11 2170 1803 3973 + U00106 10 2907 2493 5400 + U00109 20 1310 3383 4693 + U00047 10 220 1153 1373 + D00047 24 1720 2623 4343 + D00079 19 1480 1280 2760 + U00054 10 1490 927 2417 + U00110 10 13 3 17 − U00118 14 1563 2823 4387 + Rye grass 50 D00070 25 2580 1523 4103 + 11 11 100.0 U00089 12 1153 410 1563 + U00110 18 183 67 250 + U00099 20 0 2307 2307 + U00106 15 618 1786 2404 + D00004 16 1107 1607 2713 + U00117 4 253 87 340 + U00036 10 363 153 517 + U00054 15 943 403 1347 + U00125 25 403 1007 1410 + D00041 15 2060 423 2483 + Sweet vernal 50 U00099 10 1943 2960 4903 + 15 14 93.3 grass U00106 12 2443 1570 4013 + D00004 26 1907 830 2737 + U00079 7 30 23 53 − D00052 19 1543 1673 3217 + U00036 15 1313 1177 2490 + U00058 12 1087 1143 2230 + U00110 13 103 110 213 + U00102 10 143 407 550 + U00050 10 1397 860 2257 + U00054 15 273 53 327 + U00016 10 2413 1483 3897 + D00042 14 2090 1943 4033 + U00101 12 1740 63 1803 + U00075 20 1787 753 2540 + Western 50 D00070 23 1820 1950 3770 + 10 10 100.0 ragweed D00094 24 83 707 790 + D00007 11 1137 493 1630 + D00079 19 320 713 1033 + D00102 19 413 1740 2153 + D00100 16 1690 443 2133 + U00092 5 267 2020 2287 + D00045 10 563 380 943 + D00084 20 83 80 163 + U00098 7 1707 123 1830 + Total 304 286 94.1 Criteria of Average: 94.3 positivity: Total SFC ≧ 150

TABLE 7 Positive donor/peptide responses Sequence (SEQ ID NOs: 1,412-1,906, Posi- in order of Peptide Region Response (SFC/10{circumflex over ( )}6) Category Organism Antigen tion appearance) ID ID Donor IFNg IL-5 Total Fungi Alternaria Alt a 1 6 IASLFAAAGLAAAAP 3324.0015 1.01 U00129 1830 1830 rot fungus Alt a 1 6 IASLFAAAGLAAAAP 3324.0015 1.01 U00064 93 93 Alt a 1 11 AAAGLAAAAPLESRQ 3324.0017 1.01 D00003 87 87 Alt a 1 11 AAAGLAAAAPLESRQ 3324.0017 1.01 D00095 1298 1298 Alt a 1 11 AAAGLAAAAPLESRQ 3324.0017 1.01 U00064 2357 2357 Alt a 1 111 KVSDDITYVATATLP 3324.0013 1.02 U00122 197 197 Alt a 1 111 KVSDDITYVATATLP 3324.0013 1.02 D00069 53 53 Alt a 1 111 KVSDDITYVATATLP 3324.0013 1.02 D00095 1468 1468 Alt a 1 111 KVSDDITYVATATLP 3324.0013 1.02 U00064 123 123 Alt a 1 111 KVSDDITYVATATLP 3324.0013 1.02 U00075 1310 1310 Alt a 1 111 KVSDDITYVATATLP 3324.0013 1.02 U00097 712 712 Alt a 1 116 ITYVATATLPNYCRA 3324.0014 1.02 D00095 2563 2563 Alt a 1 116 ITYVATATLPNYCRA 3324.0014 1.02 D00069 327 327 Alt a 1 116 ITYVATATLPNYCRA 3324.0014 1.02 D00107 157 1123 1280 Alt a 1 116 ITYVATATLPNYCRA 3324.0014 1.02 U00064 797 797 Alt a 1 116 ITYVATATLPNYCRA 3324.0014 1.02 U00075 1405 1405 Alt a 1 116 ITYVATATLPNYCRA 3324.0014 1.02 U00097 882 882 Alt a 1 116 ITYVATATLPNYCRA 3324.0014 1.02 U00122 133 133 Alt a 1 141 QGVADAYITLVTLPK 3324.0011 1.03 D00003 623 623 Alt a 1 141 QGVADAYITLVTLPK 3324.0011 1.03 D00095 397 1768 2165 Alt a 1 141 QGVADAYITLVTLPK 3324.0011 1.03 U00064 1777 1777 Alt a 1 141 QGVADAYITLVTLPK 3324.0011 1.03 U00129 670 670 Alt a 1 141 QGVADAYITLVTLPK 3324.0011 1.03 D00107 298 298 Alt a 1 143 VADAYITLVTLPKSS 3324.0016 1.03 D00107 533 533 Alt a 1 143 VADAYITLVTLPKSS 3324.0016 1.03 D00095 1728 1728 Alt a 1 143 VADAYITLVTLPKSS 3324.0016 1.03 D00003 353 353 Alt a 1 143 VADAYITLVTLPKSS 3324.0016 1.03 U00064 1323 1323 Alt a 12 1 MSTSELATSYAALIL 3324.0036 1.04 U00075 370 1513 1883 Alt a 12 6 LATSYAALILADDGV 3324.0035 1.04 U00075 540 703 1243 Alt a 12 26 KLQSLIKAAKIEEVE 3324.0037 1.05 U00075 80 80 Alt a 13 11 QKLVLFAVKGTATST 3324.0044 1.06 U00075 70 70 Alt a 13 166 GQQYLAWLNEKFKRS 3324.0045 1.07 U00075 63 63 Alt a 5 51 INELIASGSEKLASV 3324.0065 1.08 U00075 337 337 Alt a 5 51 INELIASGSEKLASV 3324.0065 1.08 U00102 3113 3113 Alt a 6 1 MTITKIHARSVYDSR 3324.0075 1.09 U00064 47 47 Alt a 6 106 LGANAILGVSMAIAK 3324.0069 1.10 D00095 418 418 Alt a 6 116 MAIAKAAAAEKGVPL 3324.0074 1.11 U00129 63 63 Alt a 6 161 GGRLAFQEFMIVPCE 3324.0073 1.12 D00069 328 328 Alt a 6 161 GGRLAFQEFMIVPCE 3324.0073 1.12 D00107 807 103 910 Alt a 6 186 GAEVYQKLKALAKKT 3324.0072 1.13 D00069 173 173 Alt a 6 271 KSKWLTYEQLAEMYK 3324.0068 1.14 U00064 47 47 Alt a 6 306 EAWSYFFKTYDGQIV 3324.0067 1.15 U00129 90 90 Alt a 6 341 CNALLLKVNQIGTIT 3324.0071 1.16 U00129 1223 1223 Alt a 6 366 GAGWGVMVSHRSGET 3324.0078 1.17 D00003 63 63 Alt a 6 406 ERLAKLNQILRIEEE 3324.0076 1.18 U00075 1080 1080 Alt a 7 141 IYVPLGYKTAFSMLA 3324.0079 1.19 U00122 103 103 Alt a 7 190 AQGKAFYEAVAKAHQ 3324.0080 1.20 D00003 290 290 Alt a 7 190 AQGKAFYEAVAKAHQ 3324.0080 1.20 U00122 107 107 Alt a 7 36 LFQVAETLPQEVLDK 3324.0084 1.21 D00003 170 170 Alt a 7 6 AIVYYSMYGHIKKMA 3324.0085 1.22 U00075 137 137 Alt a 7 6 AIVYYSMYGHIKKMA 3324.0085 1.22 D00003 590 590 Alt a 8 236 ELKGAYVYFASDASS 3324.0088 1.23 U00075 247 247 Alt a 8 216 QDIQKLWHSMIPMGR 3324.0092 1.24 D00003 190 190 Alt a 8 151 TGSLVITSSMSGHIA 3324.0093 1.25 U00097 378 378 Aspergillus Asp f 1 146 ARVIYTYPNKVFCGI 3324.0124 1.26 U00109 318 87 405 fumigatus Asp f 12 391 KNIVKKTLELFNEIA 3324.0175 1.27 U00109 83 83 Asp f 13 256 LGGGYSYAFNNAVEN 3324.0190 1.28 U00122 442 442 Asp f 13 231 LDGFNWAVNDIVSKG 3324.0192 1.29 U00122 507 277 783 Asp f 17 116 ADSLAKAISAKVPES 3324.0209 1.30 U00050 477 477 Asp f 17 136 AQLSAGITAAIQKGI 3324.0210 1.31 U00121 171 171 Asp f 17 91 SKKDKFVAANAGGTV 3324.0212 1.32 U00113 157 157 Asp f 17 91 SKKDKFVAANAGGTV 3324.0212 1.32 U00109 657 657 Asp f 17 101 AGGTVYEDLKAQYTA 3324.0213 1.33 U00113 200 200 Asp f 22 161 GGRLAFQEFMIVPDS 3324.0252 1.34 U00050 380 380 Asp f 27 106 TNGSQFFITTVVTSW 3324.0262 1.35 U00109 87 87 Aspf 4 41 DTVYATINGVLVSWI 3324.0288 1.36 U00109 112 112 Cladosporium Cla h 10 341 SKVQFDRIMEYIQAG 3324.0560 1.37 U00110 113 113 herbarum Cla h 5 1 MKYLAAFLLLGLAGN 3324.0573 1.38 U00107 43 43 Cla h 5 6 AFLLLGLAGNSSPSA 3324.0576 1.38 U00107 103 103 Cla h 5 6 AFLLLGLAGNSSPSA 3324.0576 1.38 U00122 143 143 Cla h 6 111 ILGVSMAVAKAAAAE 3324.0586 1.39 U00128 177 177 Cla h 6 126 KRVPLYAHISDLSGT 3324.0590 1.40 U00129 67 67 Cla h 6 161 GGRLAFQEFMIVPSG 3324.0588 1.41 U00101 130 130 Cla h 6 161 GGRLAFQEFMIVPSG 3324.0588 1.41 U00110 833 157 990 Cla h 6 161 GGRLAFQEFMIVPSG 3324.0588 1.41 U00128 962 962 Cla h 6 166 FQEFMIVPSGAPSFT 3324.0581 1.41 U00128 277 277 Cla h 6 186 GAEVYQKLKSLTKKR 3324.0585 1.42 U00128 343 343 Cla h 6 281 ADQYKQLAAKYPIVS 3324.0593 1.43 U00110 53 53 Cla h 6 306 EAWSYFYKTSGSDFQ 3324.0580 1.44 D00073 70 70 Cla h 7 1 MAPKIAIIFYSTWGH 3324.0597 1.45 U00128 112 112 Cla h 7 131 AMSTLSHHGIIYVPL 3337.0019 1.46 U00128 117 117 Cla h 8 46 GAAVAITYASRAQGA 3324.0600 1.47 D00073 150 150 Cla h 8 101 QIDAFIANAGATADS 3324.0601 1.48 D00073 230 230 Cla h 8 101 QIDAFIANAGATADS 3324.0601 1.48 U00107 87 87 Cla h 8 151 GTGSLVITASMSGHI 3324.0603 1.49 U00079 420 420 Cla h 8 151 GTGSLVITASMSGHI 3324.0603 1.49 U00107 50 50 Cla h 8 181 GCIHMARSLANEWRD 3324.0602 1.50 U00079 130 130 Cla h 8 181 GCIHMARSLANEWRD 3324.0602 1.50 U00107 500 187 687 Cla h 8 236 KELKGAYVYFASDAS 3324.0598 1.51 U00079 3540 3540 Cla h 8 236 KELKGAYVYFASDAS 3324.0598 1.51 U00128 527 527 Cla h 8 241 AYVYFASDASTYTTG 3324.0599 1.51 U00079 2003 2003 Penicillium Pen ch 13 6 VLATSLATLAVVDAG 3324.1300 1.52 U00050 170 170 chrysogenum Pen ch 13 36 YIVVMNDDVSTAEFS 3324.1311 1.53 U00050 140 140 Pen ch 13 101 PAVKYIEPDMIVNAT 3324.1304 1.54 U00050 263 263 Pen ch 13 101 PAVKYIEPDMIVNAT 3324.1304 1.54 U00137 413 413 Pen ch 13 111 IVNATANVVQSNVPS 3324.1306 1.55 U00137 353 353 Pen ch 13 201 YGVAKKATLVAVKVL 3324.1305 1.56 U00137 320 320 Pen ch 13 266 NVVKSGIFLSVAAGN 3324.1299 1.57 U00050 173 173 Pen ch 13 266 NVVKSGIFLSVAAGN 3324.1299 1.57 U00137 400 400 Pen ch 13 271 GIFLSVAAGNEAENA 3324.1303 1.57 U00050 213 213 Pen ch 13 271 GIFLSVAAGNEAENA 3324.1303 1.57 U00137 1250 1250 Pen ch 13 346 APHVAGVAAYLMALE 3324.1296 1.58 U00137 333 333 Pen ch 13 371 IVQLATSSISRAPSG 3324.1302 1.59 U00137 310 310 Pen ch 18 1 MKGFLSLTLLPLLVA 3324.1314 1.60 U00109 193 193 Pen ch 18 26 NDAAPILSSMTSKDI 3324.1332 1.61 U00047 200 200 Pen ch 18 96 TFHIAGSLLGYAGHF 3324.1323 1.62 U00047 240 240 Pen ch 18 226 KFGVAKKANVYAVKV 3324.1330 1.63 U00047 360 360 Pen ch 18 226 KFGVAKKANVYAVKV 3324.1330 1.63 U00122 247 247 Pen ch 18 291 LDLAVNAAVDAGIHF 3324.1331 1.64 U00047 280 280 Pen ch 18 291 LDLAVNAAVDAGIHF 3324.1331 1.64 U00109 1237 427 1663 Pen ch 18 291 LDLAVNAAVDAGIHF 3324.1331 1.64 U00122 537 537 Pen ch 18 301 AGIHFAVAAGNDNAD 3324.1324 1.65 U00109 207 207 Pen ch 18 381 HIAGLLAYYVSLAPA 3324.1313 1.66 U00047 250 250 Pen ch 18 411 KAALISVATEGTLTD 3324.1326 1.67 U00047 320 320 Pen ch 18 476 AFHKELGAIYSEIKD 3324.1328 1.68 U00109 343 343 Pen ch 18 176 VDAYVIDTGANVKHV 3324.1327 1.69 U00132 1117 1117 Indoor American Per a 3 151 YGSIFHFYRLLVGHV 3324.1394 2.01 D00054 160 160 allergens cockroach Per a 3 191 FYQLWKRIDHIVQKY 3324.1404 2.02 D00054 350 350 Per a 3 446 YTPNMYFKDVVIFHK 3324.1415 2.03 D00054 180 180 Cat Fel d 1 1 41 DEYVEQVAQYKALPV 3324.1049 2.15 D00073 120 120 Fel d 1 1 41 DEYVEQVAQYKALPV 3324.1049 2.15 U00046 270 270 Fel d 1 1 46 QVAQYKALPVVLENA 3324.1051 2.15 U00046 2622 2622 Fel d 1 1 46 QVAQYKALPVVLENA 3324.1051 2.15 U00079 3323 3323 Fel d 1 1 51 KALPVVLENARILKN 3324.1048 2.15 U00046 660 660 Fel d 1 1 78 NALSVLDKIYTSPLC 3324.1050 2.16 D00073 587 980 1567 Fel d 1 1 78 NALSVLDKIYTSPLC 3324.1050 2.16 U00029 823 213 1037 Fel d 1 1 78 NALSVLDKIYTSPLC 3324.1050 2.16 U00079 850 850 Fel d 1 2 21 AETCPIFYDVFFAVA 3324.1056 2.17 D00073 1477 997 2473 Fel d 1 2 21 AETCPIFYDVFFAVA 3324.1056 2.17 D00069 503 503 Fel d 1 2 21 AETCPIFYDVFFAVA 3324.1056 2.17 U00046 1797 1797 Fel d 1 2 21 AETCPIFYDVFFAVA 3324.1056 2.17 U00106 557 557 Fel d 1 2 21 AETCPIFYDVFFAVA 3324.1056 2.17 U00128 570 570 Fel d 1 2 26 IFYDVFFAVANGNEL 3324.1052 2.17 U00079 903 903 Fel d 1 2 26 IFYDVFFAVANGNEL 3324.1052 2.17 U00128 115 115 Fel d 1 2 26 IFYDVFFAVANGNEL 3324.1052 2.17 U00106 1337 1337 Fel d 1 2 26 IFYDVFFAVANGNEL 3324.1052 2.17 D00069 237 237 Fel d 1 2 26 IFYDVFFAVANGNEL 3324.1052 2.17 U00046 1530 1530 Fel d 1 2 36 NGNELLLDLSLTKVN 3324.1055 2.18 U00106 100 100 Fel d 1 2 36 NGNELLLDLSLTKVN 3324.1055 2.18 D00073 1187 1550 2737 Fel d 1 2 66 YVENGLISRVLDGLV 3324.1057 2.19 U00079 183 183 Fel d 4 56 VFVEHIKALDNSSLS 3324.1090 2.20 U00029 58 58 Fel d 4 96 YTVVYDGYNVFSIVE 3324.1100 2.21 U00029 270 270 Fel d 4 116 YILLHLLNFDKTRPF 3324.1093 2.22 U00029 73 73 Dog Can f 3 156 QLFLGKYLYEIARRH 3324.0499 2.23 U00117 77 77 Can f 3 176 PELLYYAQQYKGVFA 3324.0508 2.24 U00117 53 53 Can f 3 421 KLGEYGFQNALLVRY 3324.0498 2.25 U00117 87 87 Can f 3 476 FLSVVLNRLCVLHEK 3324.0500 2.26 U00117 77 77 Can f 3 531 FTFHADLCTLPEAEK 3324.0509 2.27 U00117 60 60 D. farinae Der f 1 46 ARKNFLESLKYVEAN 3324.0668 2.28 U00117 67 67 Der f 1 86 FEQLKTQFDLNAETS 3324.0682 2.29 U00117 70 70 Der f 1 131 GSCWAFSGVAATESA 3324.0670 2.30 U00050 297 542 838 Der f 1 131 GSCWAFSGVAATESA 3324.0670 2.30 U00117 470 470 Der f 1 136 FSGVAATESAYLAYR 3324.0666 2.30 U00117 610 610 Der f 1 136 FSGVAATESAYLAYR 3324.0666 2.30 U00050 343 77 420 Der f 1 141 ATESAYLAYRNTSLD 3324.0674 2.30 U00050 217 217 Der f 1 141 ATESAYLAYRNTSLD 3324.0674 2.30 U00117 80 80 Der f 10 106 LKIATAKLEEASQSA 3324.0689 2.31 D00069 67 67 Der f 10 191 VELEEELRVVGNNLK 3324.0690 2.32 D00069 70 70 Der f 10 221 YEQQIRIMTAKLKEA 3324.0687 2.33 D00069 60 60 Der f 11 311 ELRVKIAELQKLQHE 3324.0715 2.34 U00131 433 433 Der f 13 6 GKYKLEKSEKFDEFL 3324.0727 2.35 U00124 240 240 Der f 13 26 GFMVKTAAKTLKPTF 3324.0725 2.36 U00124 2600 2600 Der f 13 46 NDQYIFRSLSTFKNT 3324.0724 2.37 U00124 487 487 Der f 13 51 FRSLSTFKNTEAKFK 3324.0726 2.37 U00124 570 570 Der f 14 196 VRPFKMHGNSDIKLM 3324.0739 2.38 U00050 647 647 Der f 16 11 IPIGHTFFFIWRIKQ 3324.0772 2.39 U00099 160 160 Der f 16 216 FKLSSVILEDGKEVE 3324.0767 2.40 U00099 177 177 Der f 16 236 EYDAFNKALSLDKKD 3324.0775 2.41 U00099 230 230 Der f 16 291 ISFVKNGPLSRADLD 3324.0776 2.42 U00099 183 183 Der f 16 366 FKSLFESWQMSEQEK 3324.0763 2.43 U00099 230 230 Der f 16 381 ITSARLFRVSRNGIF 3324.0764 2.44 U00099 223 223 Der f 16 416 VMDKIYVWIGNQFAE 3324.0766 2.45 U00099 160 160 Der f 16 451 SGRKFQPNQIIKLKQ 3324.0765 2.46 U00093 1560 1560 Der f 18 126 TFVVSTVDLMTRYGF 3324.0790 2.47 D00069 63 63 Der f 18 171 HTSFVMGVTLPATIA 3324.0789 2.48 D00069 143 143 Der f 2 121 VVVTVKLIGDNGVLA 3337.0026 2.49 D00069 63 63 D. Der p 1 11 LALSAVYARPSSIKT 3324.0828 2.50 U00050 267 77 343 pteronyssinus Der p 1 31 KAFNKSYATFEDEEA 3324.0834 2.51 U00050 1563 703 2267 Der p 1 46 ARKNFLESVKYVQSN 3324.0839 2.52 U00108 487 487 Der p 1 51 LESVKYVQSNGGAIN 3324.0838 2.53 U00117 467 467 Der p 1 61 GGAINHLSDLSLDEF 3324.0842 2.54 U00050 170 603 773 Der p 1 111 EIDLRQMRTVTPIRM 3324.0836 2.55 U00117 120 613 733 Der p 1 131 SCWAFSGVAATESAY 3324.0832 2.56 U00050 843 460 1303 Der p 1 131 SCWAFSGVAATESAY 3324.0832 2.56 U00117 233 773 1007 Der p 1 136 SGVAATESAYLAYRN 3324.0829 2.56 U00117 340 347 687 Der p 1 136 SGVAATESAYLAYRN 3324.0829 2.56 U00050 237 167 403 Der p 1 176 RGIEYIQHNGVVQES 3324.0831 2.57 U00117 567 567 Der p 1 176 RGIEYIQHNGVVQES 3324.0831 2.57 U00050 220 103 323 Der p 1 226 REALAQTHSAIAVII 3324.0840 2.58 U00050 1407 477 1883 Der p 1 226 REALAQTHSAIAVII 3324.0840 2.58 U00117 900 900 Der p 1 231 QTHSAIAVIIGIKDL 3324.0835 2.58 U00117 317 317 Der p 1 296 DNGYGYFAANIDLMM 3324.0827 2.59 U00117 53 53 Der p 14 96 FAFGYFNGRILGVCP 3324.0914 2.60 U00093 133 133 Der p 14 476 NAMDEIIKSTDAEPA 3324.0918 2.61 U00117 260 260 Der p 14 746 TGLPLMYKFGDNLVV 3324.0922 2.62 U00131 247 247 Der p 14 1346 NGKLHLSLIDPSTLS 3324.0894 2.63 U00102 460 460 Der p 2 101 QQYDIKYTWNVPKIA 3324.0940 2.64 U00117 1333 1333 Der p 3 16 TLANPILPASPNATI 3324.0969 2.65 U00108 167 167 Der p 3 61 EYWILTAAHCVAGQT 3324.0966 2.66 U00108 203 203 Der p 3 81 IRYNSLKHSLGGEKI 3324.0968 2.67 U00108 137 137 Der p 3 91 GGEKISVAKIFAHEK 3324.0964 2.68 U00108 137 137 Der p 3 111 IDNDIALIKLKSPMK 3324.0967 2.69 U00108 187 187 Der p 3 166 SELRRVDIAVVSRKE 3324.0960 2.70 U00108 120 120 Der p 4 46 FDFLLMERIHEQIKK 3324.0998 2.71 U00108 143 143 Der p 4 61 GELALFYLQEQINHF 3324.0997 2.72 U00108 1113 1113 Der p 4 86 NKAGVRIYVDIVLNH 3324.0981 2.73 U00108 93 2017 2110 Der p 4 86 NKAGVRIYVDIVLNH 3324.0981 2.73 U00128 1260 1260 Der p 4 91 RIYVDIVLNHMTGAQ 3324.0988 2.73 U00108 117 117 Der p 4 96 IVLNHMTGAQSGKGT 3324.0987 2.73 U00117 507 507 Der p 4 201 PDDLRSIYSRLHNLN 3324.0979 2.74 U00108 177 177 Der p 4 206 SIYSRLHNLNKEFFP 3324.0989 2.74 U00108 117 117 Der p 4 251 IEFRFYKEITNVFRG 3324.0976 2.75 U00108 153 153 Der p 4 251 IEFRFYKEITNVFRG 3324.0976 2.75 U00117 610 610 Der p 4 286 DALVMIDSHDLRVGH 3324.0992 2.76 U00128 290 290 Der p 4 311 FEGRLLKAATAFMLA 3324.0973 2.77 U00108 113 113 Der p 4 311 FEGRLLKAATAFMLA 3324.0973 2.77 U00117 953 953 Der p 4 316 LKAATAFMLAWNYGV 3324.0972 2.77 U00117 757 757 Der p 4 321 AFMLAWNYGVPRVMS 3324.0977 2.77 U00117 1413 1413 Der p 4 321 AFMLAWNYGVPRVMS 3324.0977 2.77 U00108 157 157 Der p 4 331 PRVMSSYFWNQIIKD 3324.0991 2.78 U00117 152 152 Der p 4 381 EHRWREIYNMVKFRM 3324.0986 2.79 U00128 300 300 Der p 4 381 EHRWREIYNMVKFRM 3324.0986 2.79 U00093 1007 1007 Der p 4 381 EHRWREIYNMVKFRM 3324.0986 2.79 U00108 80 80 Der p 4 381 EHRWREIYNMVKFRM 3324.0986 2.79 U00117 402 402 Der p 4 386 EIYNMVKFRMIAGQE 3324.0978 2.79 U00108 167 167 Der p 4 386 EIYNMVKFRMIAGQE 3324.0978 2.79 U00117 1100 1100 Der p 4 386 EIYNMVKFRMIAGQE 3324.0978 2.79 U00093 667 667 Der p 4 391 VKFRMIAGQEPVHNW 3324.0993 2.79 U00117 92 92 Der p 4 411 YQIAFSRGNRAFIAI 3324.0980 2.80 U00108 177 177 Der p 4 411 YQIAFSRGNRAFIAI 3324.0980 2.80 U00117 380 380 Der p 4 416 SRGNRAFIAINLQKN 3324.0974 2.80 U00117 940 940 Der p 4 476 YVGHDEFDAFVAYHI 3324.0975 2.81 U00117 973 973 Der p 4 481 EFDAFVAYHIGARIV 3324.0970 2.81 U00108 123 123 Der p 4 481 EFDAFVAYHIGARIV 3324.0970 2.81 U00117 763 763 Der p 4 482 FDAFVAYHIGARIVS 3324.0971 2.81 U00108 187 187 Der p 5 111 RKDLDIFEQYNLEMA 3324.1001 8.82 U00108 117 117 Der p 9 181 TLPTILQIASVTKMS 3324.1036 2.83 U00108 120 120 Der p 9 256 TIYSNVANLRNWIIS 3324.1040 2.84 U00108 297 297 Der p 9 259 SNVANLRNWIISNTV 3324.1043 2.84 U00108 177 177 Trees Alder Aln g 1 11 PSVIPAARLFKAFIL 3324.0004 3.01 D00069 683 683 Aln g 1 16 AARLFKAFILDGDKL 3324.0003 3.01 U00110 800 553 1353 Aln g 1 111 GGSILKISNKFHTKG 3324.0007 3.02 U00032 887 887 Aln g 1 111 GGSILKISNKFHTKG 3324.0007 3.02 U00110 357 357 Aln g 1 141 AVGLLKAVESYLLAH 3324.0001 3.03 D00069 597 597 Birch Bet v 1 6 YETETTSVIPAARLF 3324.0351 3.04 D00094 403 403 Bet v 1 11 TSVIPAARLFKAFFL 3324.0349 3.04 D00094 427 427 Bet v 1 76 DHTNFKYSYSVIEGG 3324.0350 3.05 D00094 413 413 Bet v 1 96 LEKISNEIKIVATPD 3324.0353 3.06 D00067 92 92 Bet v 1 96 LEKISNEIKIVATPD 3324.0353 3.06 D00094 370 370 Bet v 1 111 GGSILKISNKYHTKG 3324.0352 3.07 D00094 280 280 Bet v 1 111 GGSILKISNKYHTKG 3324.0352 3.07 D00067 612 612 Bet v 1 111 GGSILKISNKYHTKG 3324.0352 3.07 D00073 240 240 Bet v 1 146 RAVESYLLAHSDAYN 3324.0348 3.08 D00094 293 293 Bet v 2 31 DGSVWAQSSSFPQFK 3324.0355 3.09 D00094 1953 1953 Bet v 2 66 GLHLGGIKYMVIQGE 3324.0356 3.10 D00094 440 440 Bet v 2 71 GIKYMVIQGEAGAVI 3324.0354 3.10 D00094 350 350 Bet v 3 61 LSRALNLLGLETDLS 3324.0364 3.11 D00094 167 167 Bet v 6 71 VKAFKQVDVVISTVG 3324.0384 3.12 U00053 1007 1007 Bet v 6 206 RTLNKIVYIKPAKNI 3324.0387 3.13 U00053 580 580 Bet v 6 206 RTLNKIVYIKPAKNI 3324.0387 3.13 U00103 140 140 Bet v 6 211 IVYIKPAKNIYSFNE 3324.0386 3.13 U00103 227 227 Bet v 6 211 IVYIKPAKNIYSFNE 3324.0386 3.13 U00053 487 487 Bet v 6 216 PAKNIYSFNEIVALW 3324.0372 3.13 U00103 67 67 Bet v 6 261 VILAINHSVFVKGDH 3324.0380 3.14 U00103 1410 1410 Bet v 6 276 TNFEIEASFGVEASE 3324.0379 3.15 U00103 1113 1113 Bet v 6 291 LYPDVKYTTVEEYLQ 3324.0382 3.16 U00103 67 67 Cypress Cha o 1 86 ERSLWIIFSKNLNIK 3324.0517 3.17 000075 117 117 Cha o 1 86 ERSLWIIFSKNLNIK 3324.0517 3.17 D00052 97 97 Cha o 1 91 IIFSKNLNIKLNMPL 3324.0522 3.17 D00008 863 863 Cha o 1 96 NLNIKLNMPLYIAGN 3324.0523 3.17 D00008 1708 1708 Cha o 1 211 NHFFNHHKVMLLGHS 3324.0521 3.18 D00008 778 778 Cha o 1 211 NHFFNHHKVMLLGHS 3324.0521 3.18 D00052 57 57 Cha o 1 231 DKSMKVTVAFNQFGP 3324.0525 3.19 D00008 323 323 Cha o 2 6 MAAVAFLALQLIVMA 3324.0529 3.20 D00008 283 283 Cup a 1 66 KALWIIFSQNMNIKL 3324.0604 3.21 U00034 390 390 Cup a 1 71 IFSQNMNIKLQMPLY 3324.0607 3.21 D00008 2607 2607 Cup a 1 71 IFSQNMNIKLQMPLY 3324.0607 3.21 D00102 70 70 Cup a 1 76 MNIKLQMPLYVAGYK 3324.0613 3.21 D00008 652 652 Cup a 1 76 MNIKLQMPLYVAGYK 3324.0613 3.21 U00034 120 120 Cup a 1 186 TISNNHFFNHHKVML 3324.0612 3.22 D00008 542 542 Cup a 1 191 HFFNHHKVMLLGHDD 3324.0605 3.22 D00008 992 992 Cup a 1 316 EDTNIYNSNEAFKVE 3324.0606 3.23 D00008 427 427 Cup a 1 316 EDTNIYNSNEAFKVE 3324.0606 3.23 D00052 240 240 Cup a 1 316 EDTNIYNSNEAFKVE 3324.0606 3.23 D00102 73 73 Cup s 1 86 EKALWIIFSQNMNIK 3324.0615 3.24 D00052 333 67 400 Cup s 1 91 IIFSQNMNIKLKMPL 3324.0618 3.24 D00102 80 80 Cup s 1 91 IIFSQNMNIKLKMPL 3324.0618 3.24 D00008 3067 3067 Cup s 1 91 IIFSQNMNIKLKMPL 3324.0618 3.24 D00052 73 73 Cup s 1 96 NMNIKLKMPLYVAGH 3324.0624 3.24 D00008 3147 3147 Cup s 1 126 GPCLFMRKVSHVILH 3324.0619 3.25 D00008 687 687 Cup s 1 131 MRKVSHVILHGLHIH 3324.0621 3.25 D00008 642 642 Cup s 1 211 NHFFNHHKVMLLGHD 3324.0616 3.26 D00008 1427 1427 Cup s 1 211 NHFFNHHKVMLLGHD 3324.0616 3.26 D00052 173 50 223 Cup s 1 231 DKSMKVTVAFNQFGP 3324.0622 3.27 D00008 102 102 Cup s 3 11 LVATSAISLHMQEAG 3324.0630 3.28 D00102 83 83 Ash Fra e 1 21 DTCRARFITKLSEFI 3324.1102 3.29 U00047 67 67 Fra e 1 31 LSEFITGASVRLQCR 3324.1105 3.30 U00058 73 73 Fra e 1 121 LGFFKKEALPQCAQV 3324.1104 3.31 U00047 53 573 627 Prickly Jun o 4 46 ELADILRSLGSDVGE 3324.1129 3.32 U00099 380 380 juniper Jun o 4 76 GYVSLQEFVDLNNKG 3324.1131 3.33 D00068 80 80 Jun o 4 76 GYVSLQEFVDLNNKG 3324.1131 3.33 U00099 420 420 Jun o 4 146 LISVEEFQTMMTSEM 3324.1130 3.34 U00099 153 153 Date palm Pho d 2 36 SSSFPQFKSEEITNI 3324.1549 3.37 U00050 77 77 Pho d 2 71 KYMVIQGEPGAVIRG 3324.1547 3.38 U00050 63 63 Pho d 2 91 GVTVKKTNQALIFGI 3324.1550 3.39 U00050 93 93 White oak Que a 1 11 ASVIPPARLFKAFVL 3324.1587 3.40 D00026 273 273 Que a 1 11 ASVIPPARLFKAFVL 3324.1587 3.40 D00067 127 203 330 Que a 1 11 ASVIPPARLFKAFVL 3324.1587 3.40 D00078 97 97 Que a 1 11 ASVIPPARLFKAFVL 3324.1587 3.40 U00047 100 100 Que a 1 16 PARLFKAFVLDSDNL 3324.1585 3.40 U00047 170 170 Que a1 16 PARLFKAFVLDSDNL 3324.1585 3.40 U00110 1877 890 2767 Que a1 16 PARLFKAFVLDSDNL 3324.1585 3.40 U00115 1790 1790 Que a 1 16 PARLFKAFVLDSDNL 3324.1585 3.40 D00078 393 190 583 Que a1 16 PARLFKAFVLDSDNL 3324.1585 3.40 U00072 180 520 700 Que a 1 16 PARLFKAFVLDSDNL 3324.1585 3.40 D00004 70 70 Que a 1 21 KAFVLDSDNLIPKVV 3324.1588 3.40 D00004 123 123 Que a 1 21 KAFVLDSDNLIPKVV 3324.1588 3.40 U00072 200 947 1147 Que a 1 21 KAFVLDSDNLIPKVV 3324.1588 3.40 D00078 57 57 Que a1 141 ASEVFKAVEAYLVAH 3324.1584 3.41 U00047 207 207 Que a1 141 ASEVFKAVEAYLVAH 3324.1584 3.41 U00072 143 890 1033 Que a1 141 ASEVFKAVEAYLVAH 3324.1584 3.41 D00004 97 97 Que a 1 146 KAVEAYLVAHPDLYK 3324.1586 3.41 D00004 83 83 Que a 1 146 KAVEAYLVAHPDLYK 3324.1586 3.41 D00067 53 53 Que a 1 146 KAVEAYLVAHPDLYK 3324.1586 3.41 U00047 143 143 Que a 1 146 KAVEAYLVAHPDLYK 3324.1586 3.41 U00072 110 443 553 Grasses Sweet vernal Ant o 1 1 IAKVPPGPNITATYG 3324.0103 4.01 U00054 153 153 grass Ant o 1 1 IAKVPPGPNITATYG 3324.0103 4.01 U00058 223 223 Ant o 1 1 IAKVPPGPNITATYG 3324.0103 4.01 U00106 267 267 Ant o 1 16 DKWLDAKSTWYGKPT 3324.0102 4.02 U00050 447 147 593 Ant o 1 16 DKWLDAKSTWYGKPT 3324.0102 4.02 U00106 233 233 Ant o 1 16 DKWLDAKSTWYGKPT 3324.0102 4.02 U00054 123 123 Ant o 1 18 WLDAKSTWYGKPTGA 3337.0008 4.02 U00054 140 140 Ant o 1 18 WLDAKSTWYGKPTGA 3337.0008 4.02 U00075 57 57 Ant o 1 18 WLDAKSTWYGKPTGA 3337.0008 4.02 U00050 283 283 Ant o 1 18 WLDAKSTWYGKPTGA 3337.0008 4.02 U00106 163 163 Bermuda grass Cyn d 1 1 MLAVVAVVLASMVGG 3324.0633 4.03 U00089 227 227 Cyn d 12 31 TVWAQSAAFPAFKPE 3324.0639 4.04 D00104 463 463 Cyn d 12 31 TVWAQSAAFPAFKPE 3324.0639 4.04 U00054 100 100 Cyn d 7 31 LRTLGSTSADEVQRM 3324.0652 4.05 U00054 113 113 Orchard grass Dac g 1 181 PNYLALLVKYVDGDG 3324.0655 4.06 U00099 957 957 Dac g 1 181 PNYLALLVKYVDGDG 3324.0655 4.06 U00102 110 353 463 Dac g 1 181 PNYLALLVKYVDGDG 3324.0655 4.06 U00036 83 83 Dac g 1 181 PNYLALLVKYVDGDG 3324.0655 4.06 D00042 1292 1292 Dac g 2 11 GSDEKNLALSIKYNK 3324.0657 4.07 U00110 1000 1000 Dac g 2 11 GSDEKNLALSIKYNK 3324.0657 4.07 U00058 693 693 Dac g 2 16 NLALSIKYNKEGDSM 3324.0658 4.07 U00058 917 917 Dac g 2 16 NLALSIKYNKEGDSM 3324.0658 4.07 U00110 853 853 Dac g 3 82 IPTAFKIGTTYTPEE 3324.0660 4.08 U00036 110 170 280 Dac g 4 1 DIYNYMEPYVSKVDP 3324.0663 4.09 U00036 73 73 Dac g 4 1 DIYNYMEPYVSKVDP 3324.0663 4.09 U00089 302 302 Dac g 4 1 DIYNYMEPYVSKVDP 3324.0663 4.09 U00102 107 107 Rye grass Lol p 1 1 MASSSSVLLVVALFA 3324.1133 4.10 U00125 53 53 Lol p 1 11 VALFAVFLGSAHGIA 3324.1134 4.11 U00099 208 208 Lol p 1 121 EPIAPYHFDLSGHAF 3337.0028 4.12 U00106 157 97 253 Lol p 1 176 KASNPNYLAILVKYV 3324.1135 4.13 U00099 837 837 Lol p 1 176 KASNPNYLAILVKYV 3324.1135 4.13 U00106 157 100 257 Lol p 1 176 KASNPNYLAILVKYV 3324.1135 4.13 U00125 87 87 Lol p 11 106 KQQGIRYANPIAFFR 3324.1137 4.14 U00125 123 123 Lol p 11 111 RYANPIAFFRKEPLK 3324.1138 4.14 U00125 440 1697 2137 Lol p 11 116 IAFFRKEPLKECGGI 3337.0029 4.14 U00106 77 180 257 Lol p 2 51 DGVWEIKSDKPLKGP 3324.1139 4.15 U00106 103 103 Lol p 2 51 DGVWEIKSDKPLKGP 3324.1139 4.15 U00125 83 83 167 Lol p 2 51 DGVWEIKSDKPLKGP 3324.1139 4.15 D00004 577 577 Lol p 2 51 DGVWEIKSDKPLKGP 3324.1139 4.15 U00110 287 287 Lol p 2 56 IKSDKPLKGPFNFRF 3324.1140 4.15 U00110 317 317 Lol p 2 76 MRNVFDDVVPADFKV 3337.0030 4.16 U00106 103 110 213 Lol p 2 76 MRNVFDDVVPADFKV 3337.0030 4.16 D00070 133 133 Lol p 2 76 MRNVFDDVVPADFKV 3337.0030 4.16 U00125 460 447 907 Lol p 3 11 SDAKTLVLNIKYTRP 3324.1142 4.17 U00036 227 227 Lol p 3 11 SDAKTLVLNIKYTRP 3324.1142 4.17 D00004 147 147 Lol p 3 11 SDAKTLVLNIKYTRP 3324.1142 4.17 U00110 150 1547 1697 Lol p 3 51 LWEVKSAKPLTGPMN 3337.0031 4.18 U00106 90 90 Lol p 3 51 LWEVKSAKPLTGPMN 3337.0031 4.18 U00125 703 1010 1713 Lol p 3 76 NVFDEVIPTAFTVGK 3324.1141 4.19 U00099 2258 2258 Lol p 3 76 NVFDEVIPTAFTVGK 3324.1141 4.19 U00125 263 820 1083 Lol p 4 31 PENFAVVDLNQMRAV 3324.1159 4.20 U00117 75 75 Lol p 4 101 LRKYGIAAENVIDVK 3324.1145 4.21 U00117 50 50 Lol p 4 236 NEMSWIESIPFVHLG 3324.1151 4.22 U00117 1933 1933 Lol p 4 321 FPHRKGVLFNIQYVN 3324.1152 4.23 U00117 100 100 Lol p 5 1 6 HTVALFLAVALVAGP 3324.1166 4.24 U00106 53 53 Lol p 5 1 11 FLAVALVAGPAASYA 3324.1170 4.24 U00106 63 63 Lol p 5 1 71 LIEKINAGFKAAVAA 3324.1175 4.25 D00070 960 900 1860 Lol p 5 1 71 LIEKINAGFKAAVAA 3324.1175 4.25 U00099 1202 1202 Lol p 5 1 71 LIEKINAGFKAAVAA 3324.1175 4.25 U00106 263 263 Lol p 5 1 76 NAGFKAAVAAAAVVP 3324.1168 4.25 D00070 850 873 1723 Lol p 5 1 76 NAGFKAAVAAAAVVP 3324.1168 4.25 U00099 977 977 Lol p 5 1 76 NAGFKAAVAAAAVVP 3324.1168 4.25 U00106 248 248 Lol p 5 1 96 KTFVETFGTATNKAF 3324.1171 4.26 U00106 73 73 Lol p 5 1 106 TNKAFVEGLASGYAD 3324.1186 4.27 U00099 287 287 Lol p 5 1 131 DAALKLAYEAAQGAT 3324.1169 4.28 D00070 120 120 Lol p 5 1 146 PEAKYDAYVATLTEA 3324.1180 4.29 D00070 227 227 Lol p 5 1 151 DAYVATLTEALRVIA 3324.1167 4.29 D00070 1017 410 1427 Lol p 5 1 151 DAYVATLTEALRVIA 3324.1167 4.29 U00106 1743 1743 Lol p 5 1 156 TLTEALRVIAGTLEV 3324.1185 4.29 U00099 267 267 Lol p 5 1 196 VDAAYRTAATAANAA 3324.1181 4.30 U00106 383 383 Lol p 5 1 216 FTVFENTFNNAIKVS 3324.1179 4.31 U00099 87 87 Lol p 5 1 236 DSYKFIPTLVAAVKQ 3324.1165 4.32 U00099 382 382 Lol p 5 1 236 DSYKFIPTLVAAVKQ 3324.1165 4.32 U00106 78 78 Lol p 5 1 246 AAVKQAYAAKQATAP 3324.1177 4.33 U00106 43 43 Lol p 5 1 261 EVKYTVSETALKKAV 3324.1178 4.34 U00106 333 333 Lol p 5 1 271 LKKAVTAMSEAEKEA 3324.1183 4.35 U00106 128 128 Lol p 5 1 306 PAAAYATATPAAATA 3324.1184 4.36 U00106 58 58 Lol p 5 1 306 PAAAYATATPAAATA 3324.1184 4.36 U00099 1637 1637 Lol p 5 2 181 AFKIAATAANAAPTN 3324.1197 4.37 D00004 1200 1200 Lol p 5 2 181 AFKIAATAANAAPTN 3324.1197 4.37 U00106 887 887 Lol p 5 2 181 AFKIAATAANAAPTN 3324.1197 4.37 D00070 173 173 Lol p 5 2 196 DKFTVFESAFNKALN 3324.1191 4.38 U00106 97 97 Lol p 5 2 251 AALTKAITAMTQAQK 3324.1196 4.39 U00099 998 998 Canary grass Pha a 1 186 PNYLALLVKYVDGDG 3324.1428 4.40 U00099 153 132 285 Pha a 1 186 PNYLALLVKYVDGDG 3324.1428 4.40 D00052 323 323 Pha a 1 186 PNYLALLVKYVDGDG 3324.1428 4.40 D00070 147 147 Pha a 1 186 PNYLALLVKYVDGDG 3324.1428 4.40 U00029 1340 1340 Pha a 1 221 SWGAIWRIDTPDKLT 3337.0039 4.41 U00106 155 155 Pha a 5 101 DAAYRVAYEAAEGST 3324.1443 4.42 U00117 70 70 Pha a 5 121 DAFIAALTEALRVIA 3324.1432 4.43 U00029 713 713 Pha a 5 121 DAFIAALTEALRVIA 3324.1432 4.43 D00052 540 540 Pha a 5 126 ALTEALRVIAGAFEV 3324.1437 4.43 D00070 427 427 Pha a 5 166 AAFKIAATAANSAPA 3324.1441 4.44 U00029 2018 2018 Pha a 5 166 AAFKIAATAANSAPA 3324.1441 4.44 U00099 203 203 Pha a 5 181 NDKFTVFEGAFNKAI 3324.1442 4.45 D00052 400 400 Pha a 5 181 NDKFTVFEGAFNKAI 3324.1442 4.45 U00117 93 93 Pha a 5 201 GAYETYKFIPSLEAA 3324.1433 4.46 D00052 247 247 Pha a 5 201 GAYETYKFIPSLEAA 3324.1433 4.46 U00029 208 208 Pha a 5 201 GAYETYKFIPSLEAA 3324.1433 4.46 U00099 153 153 Pha a 5 206 YKFIPSLEAAVKQAY 3324.1436 4.46 D00070 513 290 803 Pha a 5 206 YKFIPSLEAAVKQAY 3324.1436 4.46 U00099 187 57 243 Pha a 5 206 YKFIPSLEAAVKQAY 3324.1436 4.46 U00029 193 193 Kentucky blue Poa p 1 121 EPIAAYHFDLSGKAF 3324.1556 4.79 D00004 297 297 grass Poa p 1 121 EPIAAYHFDLSGKAF 3324.1556 4.79 U00032 183 183 Poa p 1 121 EPIAAYHFDLSGKAF 3324.1556 4.79 U00102 877 877 Poa p 1 176 KGSNPNYLALLVKYV 3324.1557 4.80 U00102 290 290 Poa p 1 181 NYLALLVKYVTGDGD 3324.1558 4.80 U00102 80 80 Poa p 1 191 DKFTVFEAAFNNAIK 3324.1562 4.81 U00102 183 183 Poa p 5 51 QKLMEDINVGFKAAV 3324.1580 4.82 U00101 317 317 Poa p 5 51 QKLMEDINVGFKAAV 3324.1580 4.82 U00110 283 107 390 Poa p 5 56 DINVGFKAAVAAAAG 3324.1571 4.82 U00106 397 397 Poa p 5 56 DINVGFKAAVAAAAG 3324.1571 4.82 U00115 47 47 Poa p 5 56 DINVGFKAAVAAAAG 3324.1571 4.82 U00034 1057 1057 Poa p 5 56 DINVGFKAAVAAAAG 3324.1571 4.82 U00102 1183 1183 Poa p 5 61 FKAAVAAAAGAPPAD 3324.1575 4.82 U00032 138 138 Poa p 5 61 FKAAVAAAAGAPPAD 3324.1575 4.82 U00034 327 327 Poa p 5 61 FKAAVAAAAGAPPAD 3324.1575 4.82 U00102 230 230 Poa p 5 121 ATPEAKFDSFVAAFT 3324.1570 4.83 U00032 238 238 Poa p 5 121 ATPEAKFDSFVAAFT 3324.1570 4.83 U00115 80 80 Poa p 5 121 ATPEAKFDSFVAAFT 3324.1570 4.83 U00102 117 117 Poa p 5 131 VAAFTEALRIIAGVL 3324.1576 4.84 U00115 117 117 Poa p 5 131 VAAFTEALRIIAGVL 3324.1576 4.84 U00102 127 127 Poa p 5 176 AFKVAATAANAAPAN 3324.1578 4.85 U00032 127 548 675 Poa p 5 176 AFKVAATAANAAPAN 3324.1578 4.85 U00110 100 100 Poa p 5 176 AFKVAATAANAAPAN 3324.1578 4.85 U00034 107 107 Poa p 5 176 AFKVAATAANAAPAN 3324.1578 4.85 U00106 820 820 Poa p 5 176 AFKVAATAANAAPAN 3324.1578 4.85 U00102 83 83 Poa p 5 176 AFKVAATAANAAPAN 3324.1578 4.85 U00115 77 77 Poa p 5 206 ESTGGAYDTYKSIPS 3324.1583 4.86 U00115 157 157 Poa p 5 211 AYDTYKSIPSLEAAV 3324.1579 4.86 U00032 98 98 Poa p 5 211 AYDTYKSIPSLEAAV 3324.1579 4.86 U00101 163 163 Poa p 5 211 AYDTYKSIPSLEAAV 3324.1579 4.86 U00106 1320 1320 Poa p 5 221 LEAAVKQAYAATIAA 3324.1564 4.87 U00115 180 180 Poa p 5 221 LEAAVKQAYAATIAA 3324.1564 4.87 U00102 1190 1190 English Pla I 1 21 HSRNLINELSERMAG 3324.1552 5.01 D00004 118 118 plantain Pla I 1 21 HSRNLINELSERMAG 3324.1552 5.01 U00072 190 190 Pla I 1 21 HSRNLINELSERMAG 3324.1552 5.01 U00102 57 57 Pla I 1 76 IKLVKSSRPDCSEIP 3337.0044 5.02 D00004 90 90 Pla I 1 76 IKLVKSSRPDCSEIP 3337.0044 5.02 U00072 233 233 Giant Amb t 5 1 MKNIFMLTLFILIIT 3324.0099 5.03 D00002 57 57 ragweed Amb t 5 1 MKNIFMLTLFILIIT 3324.0099 5.03 D00095 77 77 Amb t 5 6 MLTLFILIITSTIKA 3324.0100 5.03 D00007 63 63 Amb t 5 6 MLTLFILIITSTIKA 3324.0100 5.03 D00096 167 167 Amb t 5 31 KQEDDGLCYEGTNCG 3337.0006 5.04 D00096 123 123 Amb t 5 56 GKYCVCYDSKAICNK 3337.0007 5.05 D00007 47 47 Russian Sal k 1 1 81 EKVKIERLHPYITLY 3324.1599 5.06 D00047 260 260 thistle Sal k 1 1 111 AAEFGTVDSATLIVE 3324.1596 5.07 D00047 3003 3003 Sal k 1 1 126 SDYFVGANLIVSNSA 3324.1594 5.08 D00047 920 920 Sal k 1 1 131 GANLIVSNSAPRPDG 3324.1601 5.08 D00047 147 147 Sal k 1 1 191 EGTVDFIFGEARSLY 3324.1590 5.09 D00047 397 1300 1697 Sal k 1 1 191 EGTVDFIFGEARSLY 3324.1590 5.09 U00106 363 363 Sal k 1 1 196 FIFGEARSLYLNTEL 3324.1591 5.09 D00047 500 500 Sal k 1 1 196 FIFGEARSLYLNTEL 3324.1591 5.09 U00106 133 133 Sal k 1 1 251 LGRAWFEAARVVFSY 3324.1592 5.10 D00047 120 1430 1550 Sal k 1 1 256 FEAARVVFSYCNLSD 3324.1593 5.10 D00047 363 363 Sal k 1 1 321 FTSLEYIEAAKWLLP 3324.1589 5.11 D00047 310 1433 1743 Sal k 1 1 321 FTSLEYIEAAKWLLP 3324.1589 5.11 U00106 203 203 Sal k 1 1 325 EYIEAAKWLLPPPKV 3324.1600 5.11 D00047 980 980 Sal k 1 2 11 RTIFFDAYLGTSYVI 3324.1602 5.12 D00047 433 433 Sal k 1 2 16 DAYLGTSYVIVIKEP 3324.1603 5.12 D00047 290 290 Sal k 2 171 KIAAEIAIALLFLRD 3324.1606 5.13 D00047 90 90 Sal k 2 176 IAIALLFLRDAKPEP 3324.1608 5.13 D00047 97 97 Sal k 2 261 SFGIILLQLLTARPP 3324.1609 5.14 D00047 103 103 Sal k 3 91 FDLYFSMARGNASLP 3324.1653 5.15 D00047 343 343 Sal k 3 191 VIAELKAAGASTIQF 3324.1632 5.16 D00047 313 313 Sal k 3 261 VTAFGFDLVRGTKTL 3324.1640 5.17 D00047 73 73 Sal k 3 306 SLATLQSLESIVGKD 3324.1647 5.18 D00047 228 228 Sal k 3 341 TKLDDEIKSWLAFAA 3324.1630 5.19 D00047 87 87 Sal k 3 376 FFSANAAALASRKSS 3324.1636 5.20 D00047 93 93 Sal k 3 621 GFYLQWAVHSFRITN 3324.1625 5.21 D00047 193 143 337

TABLE 8 HLA class II binding patterns of prevalently recognized epitope regions Region: peptide information Sequence (SEQ ID NOs: 1,907- Region Source 2,008, in order Peptide ID Organism protein Acc. no. Pos of appearance) ID 1.01 Alt. rot fungus Alt a 1 P79085 6 IASLFAAAGLAAAAP 3324.0015 1.01 Alt. rot fungus Alt a 1 P79085 11 AAAGLAAAAPLESRQ 3324.0017 1.02 Alt. rot fungus Alt a 1 P79085 111 KVSDDITYVATATLP 3324.0013 1.02 Alt. rot fungus Alt a 1 P79085 116 ITYVATATLPNYCRA 3324.0014 1.03 Alt. rot fungus Alt a 1 P7908S 141 QGVADAYITLVTLPK 3324.0011 1.03 Alt. rot fungus Alt a 1 P79085 143 VADAYITLVTLPKSS 3324.0016 1.08 Alt. rot fungus Alt a 5 P42037 51 INELIASGSEKLASV 3324.0065 1.12 Alt. rot fungus Alt a 6 Q9HDT3 161 GGRLAFQEFMIVPCE 3324.0073 1.20 Alt. rot fungus Alt a 7 P42058 190 AQGKAFYEAVAKAHQ 3324.0080 1.22 Alt. rot fungus Alt a 7 P42058 6 AIVYYSMYGHIKKMA 3324.0085 1.32 A. fumigatus Asp f 17 O60025 91 SKKDKFVAANAGGTV 3324.0212 1.38 C. herbarum Cla h 5 P42039 6 AFLLIGLAGNSSPSA 3324.0576 1.41 C. herbarum Cla h 6 P42040 161 GGRLAFQEFMIVPSG 3324.0588 1.48 C. herbarum Cla h 8 P0C0Y5 101 QIDAFIANAGATADS 3324.0601 1.49 C. herbarum Cla h 8 P0C0Y5 151 GTGSLVITASMSGHI 3324.0603 1.50 C. herbarum Cla h 8 P0C0Y5 181 GCIHMARSLANEWRD 3324.0602 1.51 C. herbarum Cla h 8 P0C0YS 236 KELKGAYVYFASDAS 3324.0598 1.54 P. chrysogenum Pen ch 13 Q9URR2 101 PAVKYIEPDMIVNAT 3324.1304 1.57 P. chrysogenum Pen ch 13 Q9URR2 271 GIFLSVAAGNEAENA 3324.1303 1.63 P. chrysogenum Pen ch 18 Q9P8G3 226 KFGVAKKANVYAVKV 3324.1330 1.64 P. chrysogenum Pen ch 18 Q9P8G3 291 LDLAVNAAVDAGIHF 3324.1331 2.15 Cat epithelia Fel d 1 1 P30438 41 DEYVEQVAQYKALPV 3324.1049 2.15 Cat epithelia Fel d 1 1 P30438 46 QVAQYKALPVVLENA 3324.1051 2.16 Cat epithelia Fel d 1 1 P30438 78 NALSVLDKIYTSPLC 3324.1050 2.17 Cat epithelia Fel d 1 2 P30440 21 AETCPIFYDVFFAVA 3324.1056 2.17 Cat epithelia Fel d 1 2 P30440 26 IFYDVFFAVANGNEL 3324.1052 2.18 Cat epithelia Fel d 1 2 P30440 36 NGNELLLDLSLTKVN 3324.1055 2.30 D. farinae Der f 1 A1YW11 131 GSCWAFSGVAATESA 3324.0670 2.30 D. farinae Der f 1 A1YW11 136 FSGVAATESAYLAYR 3324.0666 2.56 D. pteronyssinus Der p 1 P08176 131 SCWAFSGVAATESAY 3324.0832 2.56 D. pteronyssinus Der p 1 P08176 136 SGVAATESAYLAYRN 3324.0829 2.57 D. pteronyssinus Der p 1 P08176 176 RGIEYIQHNGVVQES 3324.0831 2.58 D. pteronyssinus Der p 1 P08176 226 REALAQTHSAIAVII 3324.0840 2.73 D. pteronyssinus Der p 4 Q9Y197 86 NKAGVRIYVDIVLNH 3324.0981 2.73 D. pteronyssinus Der p 4 Q9Y197 96 IVLNHMTGAQSGKGT 3324.0987 2.75 D. pteronyssinus Der p 4 Q9Y197 251 IEFRFYKEITNVFRG 3324.0976 2.77 D. pteronyssinus Der p 4 Q9Y197 321 AFMLAWNYGVPRVMS 3324.0977 2.79 D. pteronyssinus Der p 4 Q9Y197 381 EHRWREIYNMVKFRM 3324.0986 2.79 D. pteronyssinus Der p 4 Q9Y197 386 EIYNMVKFRMIAGQE 3324.0978 2.80 D. pteronyssinus Der p 4 Q9Y197 411 YQIAFSRGNRAFIAI 3324.0980 2.80 D. pteronyssinus Der p 4 Q9Y197 416 SRGNRAFIAINLQKN 3324.0974 2.81 D. pteronyssinus Der p 4 Q9Y197 476 YVGHDEFDAFVAYHI 3324.0975 2.81 D. pteronyssinus Der p 4 Q9Y197 482 FDAFVAYHIGARIVS 3324.0971 3.01 Alder Aln g 1 P38948 11 PSVIPAARLFKAFIL 3324.0004 3.01 Alder Aln g 1 P38948 16 AARLFKAFILDGDKL 3324.0003 3.02 Alder Aln g 1 P38948 111 GGSILKISNKFHTKG 3324.0007 3.06 Birch Bet v 1 O23754 96 LEKISNEIKIVATPD 3324.0353 3.07 Birch Bet v 1 O23754 111 GGSILKISNKYHTKG 3324.0352 3.13 Birch Bet v 6 Q9FUW6 206 RTLNKIVYIKPAKNI 3324.0387 3.13 Birch Bet v 6 Q9FUW6 211 IVYIKPAKNIYSFNE 3324.0386 3.17 Cypres Cha o 1 Q96385 86 ERSLWIIFSKNLNIK 3324.0517 3.17 Cypress Cha o 1 Q96385 96 NLNIKLNMPLYIAGN 3324.0523 3.18 Cypress Cha o 1 Q96385 211 NHFFNHHKVMLLGHS 3324.0521 3.21 Cypress Cup a 1 Q9SCG9 66 KALWIIFSQNMNIKL 3324.0604 3.21 Cypress Cup a 1 Q9SCG9 71 IFSQNMNIKLQMPLY 3324.0607 3.23 Cypress Cup a 1 Q9SCG9 316 EDTNIYNSNEAFKVE 3324.0606 3.24 Cypress Cup s 1 Q9M4S2 86 EKALWI1FSQNMNIK 3324.0615 3.24 Cypress Cup s 1 Q9M4S2 91 IIFSQNMNIKLKMPL 3324.0618 3.24 Cypress Cup s 1 Q9M452 96 NMNIKLKMPLYVAGH 3324.0624 3.26 Cypress Cup s 1 Q9M4S2 211 NHFFNHHKVMLLGHD 3324.0616 3.40 White oak Que a 1 B6RQS2 11 ASVIPPARLFKAFVL 3324.1587 3.40 White oak Que a 1 B6RQS2 16 PARLFKAFVLDSDNL 3324.1585 3.40 White oak Que a 1 B6RQS2 21 KAFVLDSDNLIPKVV 3324.1588 3.41 White oak Que a 1 B6RQS2 141 ASEVFKAVEAYLVAH 3324.1584 4.06 Orchard grass Dac g 1 Q7XAX7 181 PNYLALLVKYVDGDG 3324.0655 4.07 Orchard grass Dac g 2 Q41183 11 GSDEKNLALSIKYNK 3324.0657 4.07 Orchard grass Dac g 2 Q41183 16 NLALSIKYNKEGDSM 3324.0658 4.09 Orchard grass Dac g 4 P82946 1 DIYNYMEPYVSKVDP 3324.0663 4.13 Rye grass Lol p 1 P14946 176 KASNPNYLAILVKYV 3324.1135 4.14 Rye grass Lol p 11 Q7M1X5 111 RYANPIAFFRKEPLK 3324.1138 4.14 Rye grass Lol p 11 Q7M1X5 116 IAFFRKEPLKECGGI 3337.0029 4.15 Rye grass Lol p 2 P14947 51 DGVWEIKSDKPLKGP 3324.1139 4.15 Rye grass Lol p 2 P14947 56 IKSDKPLKGPFNFRF 3324.1140 4.16 Rye grass Lol p 2 P14947 76 MRNVFDDVVPADFKV 3337.0030 4.17 Rye grass Lol p 3 P14948 11 SDAKTLVLNIKYTRP 3324.1142 4.18 Rye grass Lol p 3 P14948 51 LWEVKSAKPLTGPMN 3337.0031 4.19 Rye grass Lol p 3 P14948 76 NVFDEVIPTAFTVGK 3324.1141 4.25 Rye grass Lol p 5 1 Q40237 71 LIEKINAGFKAAVAA 3324.1175 4.29 Rye grass Lol p 5 1 Q40237 151 DAYVATLTEALRVIA 3324.1167 4.29 Rye grass Lol p 5 1 Q40237 156 TLTEALRVIAGTLEV 3324.1185 4.32 Rye grass Lol p 5 1 040237 236 DSYKFIPTLVAAVKQ 3324.1165 4.36 Rye grass Lol p 5 1 Q40237 306 PAAAYATATPAAATA 3324.1184 4.37 Rye grass Lol p 5 2 Q40240 181 AFKIAATAANAAPIN 3324.1197 4.40 Canary grass Pha a 1 Q41260 186 PNYLALLVKYVDGDG 3324.1428 4.43 Canary grass Pha a 5 P56164 121 DAFIAALTEALRVIA 3324.1432 4.43 Canary grass Pha a 5 P56164 126 ALTEALRVIAGAFEV 3324.1437 4.44 Canary grass Pha a 5 P56164 166 AAFKIAATAANSAPA 3324.1441 4.45 Canary grass Pha a 5 P56164 181 NDKFTVFEGAFNKAI 3324.1442 4.46 Canary grass Pha a 5 P56164 201 GAYETYKFIPSLEAA 3324.1433 4.46 Canary grass Pha a 5 P56164 206 YKFIPSLEAAVIMAY 3324.1436 4.79 Kentucky blue Poa p 1 Q9ZP03 121 EPIAAYHFDLSGKAF 3324.1556 grass 4.82 Kentucky blue Poa p 5 Q9FPR0 51 QKLMEDINVGFKAAV 3324.1580 grass 4.82 Kentucky blue P a p 5 Q9FPR0 56 DINVGFKAAVAAAAG 3324.1571 grass 4.82 Kentucky blue Poa p 5 Q9FPR0 61 FKAAVAAAAGAPPAD 3324.1575 grass 4.83 Kentucky blue Pda p 5 Q9FPR0 121 ATPEAKFD5FVAAFT 3324.1570 grass 4.84 Kentucky blue Poa p 5 Q9FPR0 131 VAAFTEALRIIAGVL 3324.1576 grass 4.85 Kentucky blue Poa p 5 Q9FPR0 176 AFKVAATAANAAPAN 3324.1578 grass 4.86 Kentucky blue Paa p 5 Q9FPR0 206 ESTGGAYDTYKSIPS 3324.1583 grass 4.86 Kentucky blue Poa p 5 Q9FPR0 211 AYDTYKSIPSLEAAV 3324.1579 grass 4.87 Kentucky blue Poa p 5 Q9FPR0 221 LEAAVKQAYAATIAA 3324.1564 grass 5.09 Russian thistle Sal k 1 1 Q17ST3 191 EGTVDFIFGEARSLY 3324.1590 5.11 Russian thistle Sal k 1 1 Q17ST3 321 FTSLEYIEAAKWLLP 3324.1589 HLA DR binding capacity (IC₅₀ nM) Region DRB1* DRB1* DRB1* DRB1* DRB1* ID 0101 0301 0401 0404 0405 1.01 2.0 32079 9.1 5.6 1814 1.01 18 919 37 5.0 340 1.02 127 642 208 5875 234 1.02 21 5584 7.0 78 14654 1.03 151 8348 106 423 175 1.03 6.5 2797 6.3 5.0 34 1.08 6.7 32 78 71 110 1.12 238 2520 708 479 215 1.20 56 4114 60 102 200 1.22 5.2 16530 882 248 781 1.32 3.6 40660 206 1107 91 1.38 0.96 50000 7.5 683 900 1.41 486 5286 549 820 952 1.48 1.7 150 6716 6451 6807 1.49 49 3530 6.6 7.6 162 1.50 24 2757 33 7.8 128 1.51 4.8 2317 10 5806 6569 1.54 128 64 120 233 1.57 6.9 3592 28 63 942 1.63 18 9196 616 108 4648 1.64 58 780 521 78 1356 2.15 4.8 9240 41 1246 795 2.15 1.4 19151 59 6110 9334 2.16 89 320 169 97 445 2.17 766 71 442 271 556 2.17 14 5343 377 183 6079 2.18 663 19 451 102 926 2.30 42 38891 23 1616 238 2.30 8.8 5135 62 482 507 2.56 11 25798 49 267 2.56 9.6 2816 127 903 2.57 223 1145 84 341 2.58 11 4216 25 962 2.73 951 275 332 93 181 2.73 1.2 14905 6688 27 5537 2.75 7.6 516 14 583 75 2.77 1.6 49 82 628 66 2.79 131 706 170 252 331 2.79 27 665 317 84 79 2.80 1.7 117 14 27 2.80 24 615 92 415 160 2.81 19 8516 1172 1970 667 2.81 0.75 7274 248 1425 466 3.01 13 56 666 444 221 3.01 70 1069 525 706 19 3.02 19 32 84 12 126 3.06 182 1362 5260 269 1693 3.07 12 78 140 52 87 3.13 4.5 979 74 13 156 3.13 2.0 504 5.1 7.8 63 3.17 1.3 57 56 14 17 3.17 528 15393 27672 3563 3490 3.18 65 6873 208 277 157 3.21 831 4445 294 404 1484 3.21 106 86 212 245 433 3.23 25 263 617 3139 4994 3.24 64 878 312 196 427 3.24 115 568 6397 6342 8675 3.24 7.6 4211 4828 998 2537 3.26 24 22741 71 2257 573 3.40 29 778 384 43 92 3.40 98 92 16 15 28 3.40 24 7.6 4.9 49 39 3.41 0.95 778 49 7871 5866 4.06 7.8 11727 315 1084 72 4.07 110 98 2656 3379 3409 4.07 768 275 2078 2339 1425 4.09 13 50000 102 22853 5897 4.13 3.0 14228 221 3.0 124 4.14 19 1487 255 464 1961 4.14 71 1218 298 3198 413 4.15 1394 84 67 262 9793 4.15 666 2270 710 5959 8878 4.16 707 22 788 5700 1650 4.17 1552 283 9616 1376 14819 4.18 91 475 585 157 7244 4.19 292 1398 1374 4295 4254 4.25 36 190 484 341 3095 4.29 10 577 98 996 837 4.29 47 1223 110 103 6389 4.32 1.1 1654 3.9 6042 8523 4.36 0.60 11254 1.0 10 568 4.37 1.7 2824 0.75 2.1 586 4.40 6.1 12592 1032 650 42 4.43 1.5 21 7.9 4.5 2.4 4.43 19 3994 6712 101 666 4.44 0.62 1366 0.96 1.3 16 4.45 13 7870 47 71 96 4.46 1.5 2291 5.6 722 5901 4.46 1.3 532 4.2 54 6911 4.79 3.0 2447 4.7 460 6859 4.82 15 3.4 105 6410 6660 4.82 1.5 7971 13 53 615 4.82 2.2 50000 100 1254 11606 4.83 102 4730 59 1256 726 4.84 59 4940 328 348 1013 4.85 14 43392 10 6066 398 4.86 170 1197 54 4381 7674 4.86 1.7 4684 2.5 65 5999 4.87 4.1 5747 163 300 3110 5.09 1.5 11 419 21 2888 5.11 105 315 621 823 477 Peptide DRB1* DRB1* DRB1* DRB1* DRB1* DRB1* DRB1* DRB1* DRB1* ID 0701 0802 0901 1001 1101 1201 1302 1501 0162 3324.0015 1007 45 62 12 1262 10995 3251 11 871 3324.0017 532 15 128 15 924 17178 5638 148 956 3324.0013 66 151 178 291 883 7666 52 478 856 3324.0014 32 10 121 1.4 16 994 1012 1232 504 3324.0011 585 42 749 79 90 7907 3934 86 3403 3324.0016 4890 7.9 315 23 16 5300 5151 122 436 3324.0065 30 312 11 56 1312 8620 4.1 14 237 3324.0073 476 3199 782 568 6086 3836 178 344 3918 3324.0080 176 5.1 170 56 29 17697 3652 1230 2135 3324.0085 345 25 50 87 82 1639 191 1.1 491 3324.0212 35 50 12 18 716 50000 0.35 1167 350 3324.0576 21427 86 14 0.92 207 674 48 67 115 3324.0588 393 42 63 47 1050 7350 7.1 3552 380 3324.0601 1186 11 0.62 58 357 14150 0.79 146 14 3324.0603 6.3 498 202 74 262 1558 0.52 71 99 3324.0602 19 94 152 8.9 38 402 77 52 186 3324.0598 174 16 4.5 8.6 702 1169 106 71 36 3324.1304 69 6101 342 10 539 5431 23 64 515 3324.1303 90 54 57 4.0 663 26989 70 227 70 3324.1330 15 2.6 79 31 39 1160 45 61 399 3324.1331 510 135 336 830 5469 3112 1.2 1247 6275 3324.1049 62 208 107 5.8 779 76 644 8.3 99 3324.1051 77 90 37 10 499 114 1116 19 101 3324.1050 174 33 591 40 166 780 325 73 627 3324.1056 1112 1213 2567 265 7993 738 40 688 1173 3324.1052 25 114 56 81 5450 6429 35 284 555 3324.1055 945 425 408 415 6739 1806 208 840 2471 3324.0670 216 84 47 31 11595 32537 38812 178 501 3324.0666 17 78 20 34 4770 32626 958 68 65 3324.0832 70 8721 29 0.39 563 15767 50000 827 70 3324.0829 33 9532 66 7.7 647 12480 1342 52 110 3324.0831 123 783 246 61 810 654 71 178 384 3324.0840 1.8 963 4.2 61 453 1179 13 13 124 3324.0981 919 376 4205 73 2403 2559 231 70 94 3324.0987 208 94 44 14 365 5198 215 1386 4775 3324.0976 72 1.9 45 12 700 10622 79 3.9 14 3324.0977 12 5.6 5.7 54 155 903 3.8 6.5 9.2 3324.0986 295 41 1010 92 64 87 306 105 170 3324.0978 8.9 6.2 120 11 9.4 109 200 38 75 3324.0980 1.2 8491 2.5 1.7 55 881 0.15 1.7 24 3324.0974 118 69 38 4.1 1145 3589 6.1 29 114 3324.0975 0.81 3.7 11 15 68 1340 122 6.3 281 3324.0971 0.73 1.1 1.2 38 37 241 2.5 0.46 16 3324.0004 5.0 55 38 1.1 30 831 1054 1.5 150 3324.0003 9.6 224 272 3.3 3827 987 12310 4.2 354 3324.0007 113 5.3 302 42 11 1272 606 2.0 606 3324.0353 205 244 875 595 137 2320 48 318 425 3324.0352 558 6.2 92 393 15 1192 28 4.6 323 3324.0387 19 1.5 9.2 7.0 44 420 0.84 28 146 3324.0386 14 2.8 4.4 2.0 14 44 2.8 5.8 46 3324.0517 4.6 7.4 15 84 19 93 0.43 1.3 25 3324.0523 544 159 795 878 6105 5877 1.2 203 6265 3324.0521 12 22 105 124 215 2806 60 124 660 3324.0604 153 1898 222 88 3252 3488 13 582 378 3324.0607 536 30 284 175 226 52 5.9 654 1012 3324.0606 46 6524 48 580 11359 5992 0.84 100 2661 3324.0615 173 875 82 45 1519 820 15 50 195 3324.0618 115 65 958 593 143 654 15 127 1146 3324.0624 6151 21 243 125 98 206 225 10 559 3324.0616 3.4 5.7 66 14 237 2932 200 142 914 3324.1587 1.5 82 15 0.69 390 503 680 3.9 62 3324.1585 14 259 61 3.9 1005 1952 433 13 83 3324.1588 40 287 74 25 1174 1287 0.64 43 71 3324.1584 17 19 6.7 1.6 61 485 70 4.0 15 3324.0655 114 47 72 11 133 284 4777 82 177 3324.0657 439 25 851 149 452 1575 97 136 847 3324.0658 1853 37 2776 109 115 5675 116 487 1518 3324.0663 23453 311 344 9.4 748 8160 6384 70 2529 3324.1135 7.4 2.0 34 11 46 165 5.8 3.4 99 3324.1138 382 25 240 62 895 574 347 62 136 3337.0029 3495 6.8 953 280 383 8867 1666 122 472 3324.1139 30936 462 35386 8804 11353 50000 101 2247 326 3324.1140 1109 3400 2041 573 18417 8883 178 1162 961 3337.0030 443 535 9626 705 11119 36345 1132 3375 6726 3324.1142 3764 65 22025 4945 89 6223 410 2440 7796 3337.0031 360 57 836 99 2649 14985 347 664 8426 3324.1141 282 5203 2919 819 23345 11051 3895 3693 6185 3324.1175 557 343 60 26 1833 4177 57 48 247 3324.1167 13 246 72 8.5 915 1302 12 387 273 3324.1185 14 187 69 33 1724 1319 76 423 1068 3324.1165 16 6.1 6.1 4.7 23 8323 46 285 190 3324.1184 161 2.5 4.7 1.3 250 50000 3198 1529 162 3324.1197 11 3.4 8.4 0.45 84 8528 2.7 96 28 3324.1428 173 42 82 21 165 269 144 92 196 3324.1432 1.1 66 6.8 0.37 33 11 12 1.3 7.6 3324.1437 8.4 60 71 28 263 212 69 147 815 3324.1441 6.8 1.7 2.9 0.91 14 2382 1.8 29 23 3324.1442 8.4 166 24 1.6 723 9786 171 8.3 33 3324.1433 15 19 13 2.3 83 735 661 45 341 3324.1436 21 60 25 9.4 130 1030 704 685 71 3324.1556 28 65 7.8 30 588 704 1774 2.6 108 3324.1580 474 315 58 195 634 10790 5.1 44 156 3324.1571 37 4.8 8.9 7.3 619 7888 621 12 308 3324.1575 101 43 5.6 13 2286 7531 3458 519 334 3324.1570 98 240 65 31 13650 12428 109 67 875 3324.1576 41 42 101 83 73 1212 262 436 734 3324.1578 52 49 73 3.2 325 13571 108 852 577 3324.1583 519 189 514 56 5761 50000 815 573 335 3324.1579 57 17 39 4.1 29 19306 1894 61 5.7 3324.1564 1.8 317 5.3 147 3363 5474 0.27 50 2335 3324.1590 14 105 9.1 278 1641 772 258 18 1982 3324.1589 177 528 382 98 1307 6237 62 544 5053 Peptide DRB3* DRB3* DRB4* DRB5* ID 0101 0202 0101 0101 3324.0015 46138 4645 301 746 3324.0017 50000 1043 28 135 3324.0013 6000 5275 1159 1756 3324.0014 1325 8.7 4417 68 3324.0011 3672 3832 627 81 3324.0016 13047 324 58 8.3 3324.0065 3736 8.5 193 138 3324.0073 12846 2123 24 2781 3324.0080 32587 3230 548 38 3324.0085 21576 2686 186 4.3 3324.0212 203 783 6746 129 3324.0576 50000 29 98 363 3324.0588 8985 1087 4.0 3599 3324.0601 31 3.5 920 3843 3324.0603 5715 1554 594 3279 3324.0602 3902 61 270 122 3324.0598 90 696 8.3 904 3324.1304 1413 926 4.9 363 3324.1303 10285 96 107 75 3324.1330 19072 1165 36 38 3324.1331 56 10 4.6 19692 3324.1049 3223 425 2106 15 3324.1051 7529 1007 1739 902 3324.1050 287 2461 157 1112 3324.1056 240 5759 2410 17988 3324.1052 889 941 50000 506 3324.1055 103 1870 406 1633 3324.0670 50000 524 38259 3721 3324.0666 50000 13194 8441 823 3324.0832 50000 615 3875 503 3324.0829 50000 2256 454 611 3324.0831 50000 1020 223 3872 3324.0840 50000 3764 438 2201 3324.0981 116 4601 113 7898 3324.0987 50000 236 118 799 3324.0976 69 3489 463 15 3324.0977 855 7.2 57 25 3324.0986 5036 1745 48 5.4 3324.0978 6603 1665 163 285 3324.0980 1125 6.1 4.9 9.4 3324.0974 1497 6.0 3.7 52 3324.0975 1238 2680 107 39 3324.0971 5339 19 743 1.6 3324.0004 1657 2038 67 213 3324.0003 75 4320 30 57 3324.0007 13748 3140 66 15 3324.0353 4859 779 320 1637 3324.0352 4855 5846 202 36 3324.0387 530 16 4.6 24 3324.0386 268 2.4 1.1 18 3324.0517 1218 112 71 106 3324.0523 7305 378 718 9501 3324.0521 10863 557 24 2408 3324.0604 2626 275 128 582 3324.0607 503 3.0 0.23 1960 3324.0606 105 25 7518 1139 3324.0615 2736 706 12 2176 3324.0618 2187 5.6 626 200 3324.0624 18149 1152 152 639 3324.0616 7250 549 11 2201 3324.1587 1634 2477 30 218 3324.1585 6.2 1094 14 502 3324.1588 1.0 15 892 65 3324.1584 253 8.8 576 36 3324.0655 34528 674 415 587 3324.0657 14129 1034 1803 1268 3324.0658 6679 4791 4795 3590 3324.0663 26539 28096 17451 190 3324.1135 1513 9.5 83 139 3324.1138 3842 2250 562 52 3337.0029 915 11381 1770 109 3324.1139 719 18619 6923 1638 3324.1140 1470 1642 6585 33683 3337.0030 855 8286 7473 1392 3324.1142 22005 5168 2463 20103 3337.0031 24579 24793 31 792 3324.1141 9896 5681 148 37968 3324.1175 8694 126 907 10 3324.1167 223 52 342 200 3324.1185 4464 1511 429 3337 3324.1165 1680 8.8 1454 16 3324.1184 10938 183 320 75 3324.1197 17319 62 67 144 3324.1428 5040 876 98 1102 3324.1432 13 8.5 73 0.36 3324.1437 13812 27576 113 323 3324.1441 738 0.61 11 6.9 3324.1442 631 82 475 0.51 3324.1433 1088 125 992 74 3324.1436 1738 107 828 27 3324.1556 1.4 92 0.12 87 3324.1580 4.7 41 82 561 3324.1571 3192 864 796 111 3324.1575 14700 2418 3857 140 3324.1570 2253 3475 85 683 3324.1576 17847 1590 73 2246 3324.1578 50000 95 374 460 3324.1583 513 1381 11481 3460 3324.1579 7820 177 4373 104 3324.1564 356 1308 11 234 3324.1590 198 939 5972 114 3324.1589 122 744 2140 164 HLA DQ binding capacity (IC50 nM) Peptide DQB1* DQB1* DQB1* DQB1* DQB1* DQB1* DQB1 DQB1* DQB1* ID 0201 0202 0301 0302 0402 0501 0502 0503 0602 3324.0015 4120 1055 2.8 3010 2402 24056 7019 7261 103 3324.4017 1853 50000 1.2 393 118 50000 8005 223 21 3324.0013 123 8362 1144 2052 543 23106 25813 19392 2143 3324.0414 823 3845 59 764 170 17589 8971 14205 2232 3324.0011 1812 31720 1315 1045 459 8010 6014 9102 4947 3324.0416 570 7017 370 1743 1061 5103 7746 1117 70 3324.0065 5008 6578 72 4893 1877 34647 25918 7535 302 3324.0073 299 32061 710 564 1183 23 178 4943 1289 3324.0480 8948 50000 694 2674 1674 345 1365 157 2356 3324.0085 5789 6241 417 32021 50000 392 3255 1662 3352 3324.4212 5873 3328 16 2657 416 50000 1381 162 26891 3324.0576 3864 8356 12 11230 66 5484 718 557 792 3324.0588 1989 50000 2502 2619 2807 68 4947 2437 1515 3324.0601 482 3501 17 1024 57 11040 819 84 737 3324.0603 3922 114 31 11509 5287 6135 27045 13832 484 3324.0602 1048 301 41 1650 116 3078 673 649 174 3324.0598 306 1225 23 1472 781 1043 4672 518 12 3324.1304 47 283 822 1314 119 1961 1786 549 3324.1303 121 4717 33 52 102 3957 50000 50000 370 3324.1330 994 9490 114 12547 1199 28997 10107 8157 1583 3324.1331 87 361 19 138 12 128 583 1149 480 3324.1049 512 630 5346 1188 806 147 337 2523 140 3324.1051 661 21374 5072 150 46 434 552 610 1596 3324.1050 2628 1593 6450 3675 611 687 3312 5835 1000 3324.1056 132 2101 108 209 75 36 464 263 2273 3324.1052 198 28703 19 320 329 872 6652 8127 1134 3324.1055 2585 8017 3528 1981 3942 629 2865 9367 10904 3324.0670 97 5447 24 249 84 5340 9320 7931 1334 3324.0666 65 156 44 1137 76 3429 1209 4191 2016 3324.0832 92 21 61 34 6535 4402 8426 136 3324.0829 210 871 3429 2855 496 2997 5141 1136 3324.0831 263 32412 1188 723 8536 1139 12278 859 3324.0840 536 629 1377 1848 436 2459 32124 291 3324.0981 2215 15466 1201 4845 12990 54 4508 8099 5125 3324.0987 29771 50000 228 8351 3184 26479 50000 12133 65 3324.0976 227 6202 531 2740 2505 668 5519 4366 318 3324.0977 698 8364 8.6 2475 535 192 992 339 762 3324.0986 3886 21738 10587 7236 39494 346 8936 4244 4799 3324.0978 2104 29098 453 799 838 3705 26552 11155 368 3324.0980 164 8722 304 194 853 101 1406 12 3324.0974 364 7387 750 487 125 150 546 84 98 3324.0975 113 8141 986 198 51 151 5043 1188 1056 3324.0971 2735 4750 56 3358 1265 219 666 1079 54 3324.0004 2592 11232 13 9502 3892 1218 325 1451 198 3324.0003 123 4170 1739 916 426 60 63 827 910 3324.0007 13610 50000 557 31293 50000 6829 397 5806 8441 3324.0353 67 8672 1337 832 2035 3613 5095 8893 19 3324.0352 3537 5376 642 32210 18603 871 1033 1170 6058 3324.0387 1075 12465 1060 8820 1240 5585 1272 3461 58 3324.0386 1867 1491 121 6361 2791 1876 5835 650 19 3324.0517 3172 538 638 5593 27574 729 739 301 432 3324.0523 10464 39726 31919 50000 30440 6387 9115 10733 2864 3324.0521 7924 8395 3764 10232 17866 3978 5872 2358 48 3324.0604 8265 36682 883 20699 28918 5797 50000 29047 3187 3324.0607 684 918 710 827 1043 4578 4714 4899 262 3324.0606 111 7541 1212 711 163 12904 2416 11286 8286 3324.0615 2962 5556 7955 7270 6143 888 12985 1930 1344 3324.0618 37377 24912 38760 50000 6739 25974 32436 35955 1863 3324.0624 50000 50000 3057 50000 27874 33698 20832 11703 14453 3324.0616 7308 28117 2356 30130 35059 3323 4140 4158 179 3324.1587 2114 6727 38 4693 3295 1321 636 1948 267 3324.1565 12 310 1472 167 71 22 7.9 161 2603 3324.1588 116 194 4575 3941 900 302 162 2639 864 3324.1584 308 953 88 423 240 1252 5307 2036 183 3324.0655 1131 3444 382 904 1191 629 2011 6210 330 3324.0657 26713 7196 2419 9020 6027 30986 739 50000 130 3324.0658 1225 2121 6011 5470 2137 10389 3871 8965 258 3324.0663 4569 6212 9809 13492 3874 188 2016 981 12122 3324.1135 1405 5443 805 5850 1810 627 1244 1316 667 3324.1138 4460 25959 5721 32683 5739 215 793 1597 9744 3337.0029 27269 26168 8997 50000 1829 50000 1441 903 50000 3324.1139 1068 530 698 39629 9885 50000 9236 50000 26620 3324.1140 4976 2241 33901 50000 39533 8398 38172 50000 50000 3337.0030 646 148 20336 50000 622 50000 61 347 39533 3324.1142 19328 25717 26715 19079 10584 50000 50000 33924 28683 3337.0031 4656 2441 48 50000 846 50000 2336 11264 3946 3324.1141 1816 1696 10397 7083 1266 12367 17499 2899 50000 3324.1175 3075 50000 20 454 215 83 430 147 24 3324.1167 707 1800 1118 543 125 50000 50000 50000 4717 3324.1185 278 2248 132 854 40 6132 9812 4648 110 3324.1165 943 5863 73 448 180 21294 50000 4637 962 3324.1184 1766 679 3.8 700 102 11352 7446 1738 224 3324.1197 431 2314 4.2 277 138 16916 21605 6721 31 3324.1428 459 11971 1009 1243 2753 740 8618 50000 675 3324.1432 97 2038 2.5 2.3 230 610 24876 50000 70 3324.1437 132 4668 75 189 151 4412 3107 181 23 3324.1441 237 1172 7.1 457 83 3079 982 86 25 3324.1442 2630 1194 211 2614 1302 3407 966 2540 429 3324.1433 515 163 81 72 36 191 70 358 846 3324.1436 2114 2125 839 2370 640 9969 2647 1029 732 3324.1556 580 2173 143 4634 274 563 680 79 3426 3324.1580 9101 8072 1567 2884 785 1009 3189 5214 1291 3324.1571 2268 9550 5.5 651 137 631 5410 663 34 3324.1575 3690 25684 1.9 1153 322 11872 6782 1380 793 3324.1570 1026 5123 628 197 24 2818 8000 910 3192 3324.1576 856 18825 844 11612 10345 1862 50000 10912 471 3324.1578 1523 3198 32 2854 314 6808 50000 21644 144 3324.1583 7961 2754 938 7772 4639 6583 18032 5811 1029 3324.1579 1040 1496 407 183 44 5384 8336 5319 96 3324.1564 1660 5850 38 1230 475 3581 10001 890 146 3324.1590 734 3116 46 952 412 2582 6849 2356 1337 3324.1589 412 2977 1933 6628 2521 6188 19481 16501 1296 HLA DP binding capacity (IC50 nM) Peptide DPB1* DPB1* DPB1* DPB1* DPB1* DPB1* DPB1* DPB1* ID 0101 0201 0301 0401 0402 0501 1401 2001 3324.0015 6156 2492 61 28038 582 3718 234 2089 3324.4017 3370 357 71 40712 19838 463 125 184 3324.0013 1768 18050 685 1614 3389 1286 4372 12845 3324.0414 988 969 241 2994 2258 1867 1139 200 3324.0011 444 7728 16881 2009 509 72 3388 8828 3324.0416 40 11996 1124 2501 1065 7.1 1432 8647 3324.0065 527 2734 3549 2738 1177 329 50 7505 3324.0073 47 35 5073 51 69 477 3088 7642 3324.0480 39 104 36 412 685 45 938 77 3324.0085 33 32 107 72 145 47 398 1294 3324.4212 22256 2956 1824 11512 50000 5627 7611 2587 3324.0576 1437 112 406 1910 232 8188 4054 143 3324.0588 202 57 1091 37 268 83 9666 29599 3324.0601 6173 2559 8817 1880 19500 36023 1876 2378 3324.0603 12974 26959 129 7104 10715 8303 36 1562 3324.0602 5570 8054 695 21959 32474 6254 3885 1270 3324.0598 181 57 1578 60 537 1556 2384 1247 3324.1304 234 1013 91 389 170 503 3324.1303 549 108 5.0 204 3667 6490 70 146 3324.1330 1773 501 812 5008 4599 3553 50000 2723 3324.1331 6381 5052 273 9176 6632 8028 43033 5533 3324.1049 1670 1120 1036 533 26784 16357 1149 17743 3324.1051 3517 1808 14 14857 20869 3802 65 806 3324.1050 251 575 3147 349 32 152 3094 1360 3324.1056 3.1 8.1 1740 5.6 28 520 92 3965 3324.1052 3268 89 344 242 5175 5558 3247 1232 3324.1055 180 841 5014 590 844 57 1445 15456 3324.0670 9878 9311 18 14425 6224 17163 13775 396 3324.0666 8430 8262 276 1443 20587 8843 6182 1186 3324.0832 1362 20 3908 9552 652 7.7 3324.0829 1205 1601 20081 4927 9417 449 3324.0831 1625 4341 7397 35246 50000 726 3324.0840 232 1017 32 3999 1219 310 3324.0981 258 220 2.5 2722 1368 1602 1317 17 3324.0987 15476 2227 505 16086 25757 3452 15612 2929 3324.0976 419 35 123 3334 14 45 905 247 3324.0977 1638 185 988 5157 907 5066 2674 1004 3324.0986 324 109 92 549 3087 106 6551 585 3324.0978 583 5.3 4120 122 3322 2233 7868 9005 3324.0980 400 8.4 1514 1394 703 6.9 3324.0974 9.1 7.2 0.67 37 15 13 4280 3.5 3324.0975 323 177 24 273 83 76 32 379 3324.0971 173 12 286 71 66 778 11410 509 3324.0004 85 63 820 7275 1500 493 38 1824 3324.0003 388 36 1879 1810 8114 54 1021 2570 3324.0007 391 843 17488 18503 918 3.3 1042 50000 3324.0353 4094 7568 5614 6815 2700 336 5983 5918 3324.0352 279 4651 12307 10528 1391 2.2 7590 12980 3324.0387 128 70 0.039 41 92 157 3767 3.3 3324.0386 1612 1352 5.1 5907 6715 1054 2923 178 3324.0517 13 13 3.1 92 275 11 2884 83 3324.0523 1618 5002 96 11533 16167 7505 525 4965 3324.0521 334 235 24 760 1461 599 3308 895 3324.0604 6935 1199 1602 1734 12055 2370 29 23870 3324.0607 692 819 7136 4187 1207 748 3578 13550 3324.0606 8235 7270 39613 13781 18705 14225 155 25475 3324.0615 676 1062 1170 1538 4707 1147 3563 9177 3324.0618 806 304 50000 1594 75 179 6245 35360 3324.0624 2364 18 7024 2078 991 3307 6585 25735 3324.0616 104 92 6026 561 3137 633 2140 10277 3324.1587 114 28 326 917 1062 341 3333 1167 3324.1565 17 36 30 52 92 80 2136 274 3324.1588 141 171 2489 111 97 242 4441 3488 3324.1584 21 46 133 51 73 31 40 1305 3324.0655 1656 279 50000 5690 666 500 7748 12339 3324.0657 557 672 3068 2847 641 144 8095 6268 3324.0658 1351 2426 18010 3779 799 717 31957 21638 3324.0663 50000 50000 50000 23361 9581 1437 4557 50000 3324.1135 114 103 2899 383 569 65 1052 13026 3324.1138 205 110 764 2058 849 0.25 1171 223 3337.0029 158 80 1407 53 786 51 18120 8.1 3324.1139 18555 50000 1266 5209 50000 50000 1295 7941 3324.1140 469 9542 483 12300 19660 12418 560 5375 3337.0030 4060 1987 685 2929 50000 2212 24225 82 3324.1142 1420 5439 11767 7261 11975 19 21639 23992 3337.0031 12984 7712 6241 2618 26311 5538 18603 671 3324.1141 6266 3859 5633 4342 30123 9031 16711 13196 3324.1175 3337 251 339 18310 41527 258 2418 4991 3324.1167 7236 707 78 515 41 76 4399 597 3324.1185 121 3168 469 1615 1561 777 4125 4699 3324.1165 79 69 2.5 86 34 6.9 4652 12 3324.1184 5959 3491 81 21724 36457 12422 299 611 3324.1197 2717 5456 0.78 8768 10255 44 0.70 2.1 3324.1428 4818 119 8498 1760 1404 388 34437 50000 3324.1432 7.9 7.4 275 8.0 3.0 20 29689 31 3324.1437 872 5184 387 849 3350 4358 9.6 1139 3324.1441 675 181 0.84 3480 8061 103 1201 1.4 3324.1442 613 28 22 177 4312 141 444 97 3324.1433 51 113 15 76 92 306 7.2 1266 3324.1436 5958 272 38 1259 6874 22 285 1655 3324.1556 2125 68 329 1331 5002 435 183 1038 3324.1580 787 160 595 1078 2614 15 69 2183 3324.1571 5339 1467 67 20678 50000 436 2.6 295 3324.1575 24797 9228 39 28919 25866 2027 37 2935 3324.1570 3256 2266 882 4846 20193 7.4 2772 3237 3324.1576 1518 1560 945 982 995 800 12 5142 3324.1578 675 1386 6.8 854 1593 297 297 13 3324.1583 566 941 3829 370 8774 1112 75 4830 3324.1579 67 894 21 803 1132 496 485 352 3324.1564 3569 1147 7.6 1071 5202 891 2003 84 3324.1590 922 495 451 2043 2663 1311 1918 558 3324.1589 56 1782 438 568 1338 275 229 4884

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1. (canceled)
 2. A protein or peptide comprising, consisting of or consisting essentially of an amino acid sequence set forth in Table 5 (SEQ ID NOs: 1-1,411, respectively, in order of appearance), Table 7 (SEQ ID NOs: 1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs: 1,907-2,008, respectively, in order of appearance), or a variant or derivative thereof.
 3. The protein or peptide of claim 2, wherein the variant is: a. a peptide of up to 30 amino acids in length and which comprises said amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance); or b. a peptide from 7 to 30 amino acids in length, wherein at least 7 contiguous amino acids have at least 75% identity to at least 7 contiguous amino acids of said amino acid sequence set forth in Table 7 (SEQ ID NOs:1,412-1,906, respectively, in order of appearance).
 4. The protein or peptide of claim 2, wherein the protein or peptide does not consist of the sequence of Alt a 6 (SEQ ID NO:1455, Asp f l(SEQ ID NO:1466), Fel d 1 1 (SEQ ID NOs:1531, 1532, 1533, 1534, 1535 1536, 1537, 1538), Fel d 1 2 (SEQ ID NOs:1539, 1540, 1541, 1542, 1543, 1544, 1545, 1546, 1547, 1548, 1549, 1550, 1551), Can f 3 (SEQ ID NOs:1557), Der f 1 (SEQ ID NOs: 1562, 1563, 1564, 1565), Der f 2 (SEQ ID NO:1587), Der p1 (SEQ ID NOs: 1591, 1593, 1594, 1595, 1596, 1597, 1598, 1599,
 1600. 1601, 1602, 1603), Der p 2 (SEQ ID NO: 1608), Aln g 1 (SEQ ID NOs:1653, 1654, 1655), Bet v 1 (SEQ ID NOs: 1656, 1657, 1658, 1659, 1660, 1661, 1662, 1663, 1664), Bet v 2 (SEQ ID NO: 1665), Cha o 1 (SEQ ID NOs: 1678, 1679, 1680, 1681, 1682, 1683, 1684), Cup a 1 (SEQ ID NOs: 1689, 1691, 1692), Cup s 1 (SEQ ID NOs: 1700, 1701), Jun o 4 (SEQ ID NO: 1710), Ant o 1 (SEQ ID NO: 1743), Lol p 1 (SEQ ID NOs: 1765, 1766, 1767, 1768), Lol p 11 (SEQ ID NO: 1770), Lol p 5 1 (SEQ ID NOs: 1793, 1794, 1795, 1796, 1797, 1798, 1802, 1803, 1804, 1805, 1807, 1808, 1809, 1811, 1812), Lol p 5 2 (SEQ ID NOs: 1815, 1816, 1817, 1818, 1819), Pha a 1 (SEQ ID NO:1824), Pha a 5 (SEQ ID NOs:1831, 1833, 1836), Poa 1 (SEQ ID NOs: 1842, 1844), Poa p 5 (SEQ ID NOs: 1845, 1846, 1847, 1848, 1849, 1850, 1851, 1852, 1853, 1854, 1855, 1856, 1859, 1860, 1861, 1862, 1863, 1864, 1865, 1866, 1867, 1868, 1869, 1870) or Amb 1 5 (SEQ ID NO: 1881) as set forth in Table
 7. 5. The protein or peptide of claim 2, wherein the protein or peptide is derived from a house dust mite allergen.
 6. (canceled)
 7. The protein or peptide of claim 2, wherein the amino acid sequence, variant or derivative thereof i) elicits, stimulates, induces, promotes, increases or enhances an anti-allergen immune responses; decreases, inhibits, suppresses or reduces an anti-allergen immune response; ii) decreases, inhibits, suppresses or reduces an anti-allergen immune response; iii) elicits, stimulates, induces, promotes, increases or enhances a T cell response to production of Ige Antibody; iv) decreases, inhibits, suppresses or reduces a T cell response or production of IgE Antibody; v) modulates a Th2 immune response; vi) modulates production of a lymphokine or cytokine by a cell; vii) desensitizes, or improves, increases, or induces immunological tolerance of a subject to the allergen; or viii) modulates production of IL-5 (interleukin-5), IL-4 (interleukin-4), IL-10 (interleukin-10), m IL-13 (interleukin 13), IL-17 (interleukin-17, or IFN (interferon-gamma). 8-18. (canceled)
 19. The protein or peptide of claim 2, wherein the amino acid sequence, variant or derivative thereof is derived from an allergen or antigen produced by Alder, Alternaria rot fungus, American cockroach, Dust mite, Ash, Aspergillus fumigatus, Bermuda grass, Birch, Black Walnut, Canary grass, Cat, Cladosporium herbarum, Common cypress, Cypress, Palm, Dog, English plantain, Giant ragweed, Japanese cypress, Kentucky blue grass, Lolium perenne, Mugwort weed, Orchard grass, Penicillium chrysogenum, Prickly juniper, Russian thistle, Rye grass, Sweet vernal grass, Western Ragweed or White oak.
 20. The protein or peptide of claim 2, wherein the amino acid sequence has a length in a range of about 5-10, 10-15, 15-20, 20-25, 25-30, 30-35, 35-40, 45-50, 50-60, 60-70, 70-80, 90-100, 100-125, 125-150, 150-175, 175-200, 200-250, 250-300, or more amino acid residues. 21-22. (canceled)
 23. The protein or peptide of claim 2, wherein the amino acid sequence, variant or derivative thereof is immobilized on a substrate. 24-26. (canceled)
 27. A composition comprising the protein or peptide of claim
 2. 28. The composition of claim 27, wherein the composition is a pharmaceutical composition.
 29. (canceled)
 30. The composition of claim 27, wherein the composition is a vaccine composition. 31-36. (canceled)
 37. A method of modulating immune activity of a cell against an allergen, the method comprising contacting the cell with an amount of the protein or peptide of claim 2, sufficient to modulate the immune activity of the cell against the allergen.
 38. The method of claim 37, wherein the method comprises contacting the cell with the protein or peptide multiple times. 39-46. (canceled)
 47. A method of desensitizing or inducing, eliciting, increasing or improving immunological tolerance of a subject to a protein or peptide allergen comprising administering to the subject an amount of the protein or peptide of claim 2 sufficient to desensitize or induce, elicit, increase or improve tolerance of the protein or peptide allergen by the subject. 48-50. (canceled)
 51. The method of claim 47, wherein the method comprises administering the protein of peptide to the subject multiple times. 52-67. (canceled)
 68. A method of detecting an immune response in a subject, the method comprising: i. contacting a cell from the subject with a protein or peptide of claim 2; and ii. determining if the protein or peptide modulates an immune response or activity from the contacted cell; wherein determination that the protein or peptide modulates an immune response or activity from the contacted cell detects an allergic response or indicates that the subject has an allergic response or an allergy. 69-76. (canceled)
 77. A kit, comprising a compartment and instructions, a) wherein the compartment comprises: i) one or more amino acid sequences set forth in Table 5 (SEQ ID NOs: 1-1, 411, respectively, in order of appearance), Table (SEQ ID NOs: 1,412-1,906, respectively, in order of appearance) or Table 8 (SEQ ID NOs: 1,907-2,008, respectively, in order of appearance), or a variant or derivative thereof, or ii) one or more proteins or peptides of claim 2; and b) wherein the instructions are for use in any of: iii) modulating an immune response or activity of a cell against an allergen; iv) modulating an immune response against an allergen in a subject; v) desensitizing, or inducing, eliciting, increasing or improving immunological tolerance to a protein or peptide allergen; vi) reducing risk or providing a subject protection against an allergic reaction, allergic response, allergic disorder or allergic disease; vii) treating an allergic reaction, allergic response, allergic disorder or allergic disease; or viii) detecting an allergic response or diagnosing an allergy in a subject.
 78. The composition of claim 27, wherein the protein or peptide is isolated or purified.
 79. The composition of claim 27, further comprising a pharmaceutically acceptable carrier, excipient and/or adjuvant, optionally sterile.
 80. The composition of claim 27, wherein the composition is a powder, for example in freeze-dried form, optionally sterile. 